(Circular) (Six-base) MAPSORT of: pFN21AA1816.seq Check: 4132 from: 1 to: 8104 pFN21AA1816 8104 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5914 278 Size: 53 83 186 936 4378 2468 Fragments arranged by size: 4378 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5447 1937 Size: 3510 4594 Acc65I G'GTAC_C Cuts at: 2286 5425 2286 Size: 3139 4965 AclI AA'CG_TT Cuts at: 1563 6231 6604 1563 Size: 4668 373 3063 Fragments arranged by size: 4668 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2628 6167 7551 7819 1632 Size: 996 3539 1384 268 1917 Fragments arranged by size: 3539 1917 1384 996 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7907 AflIII A'CryG_T Cuts at: 1203 3106 3387 5262 7003 7980 1203 Size: 1903 281 1875 1741 977 1327 Fragments arranged by size: 1903 1875 1741 1327 977 281 AgeI A'CCGG_T Cuts at: 6935 6935 Size: 8104 AhdI GACnn_n'nnGTC Cuts at: 6833 6833 Size: 8104 AleI CACnn'nnGTG Cuts at: 1166 2222 1166 Size: 1056 7048 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2556 1184 Size: 1372 6732 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2695 2727 2695 Size: 32 8072 AlwNI CAG_nnn'CTG Cuts at: 2986 3041 3365 4267 5005 5847 7419 2986 Size: 55 324 902 738 842 1572 3671 Fragments arranged by size: 3671 1572 902 842 738 324 55 ApaI G_GGCC'C Cuts at: 2183 4237 2183 Size: 2054 6050 ApaLI G'TGCA_C Cuts at: 6161 7317 6161 Size: 1156 6948 ApoI r'AATT_y Cuts at: 1493 2554 2577 3023 5413 5639 5791 7900 1493 Size: 1061 23 446 2390 226 152 2109 1697 Fragments arranged by size: 2390 2109 1697 1061 446 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 2910 2939 6658 160 Size: 872 1878 29 3719 1606 Fragments arranged by size: 3719 1878 1606 872 29 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8104 AvaI C'yCGr_G Cuts at: 1870 1943 2407 5092 5429 1870 Size: 73 464 2685 337 4545 Fragments arranged by size: 4545 2685 464 337 73 AvrII C'CTAG_G Cuts at: 4811 4811 Size: 8104 BamHI G'GATC_C Cuts at: 5434 5801 5434 Size: 367 7737 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2262 2286 2381 2387 4943 Size: 325 217 627 475 24 95 6 2556 Cuts at: 4943 5105 5425 6880 618 Size: 162 320 1455 1842 Fragments arranged by size: 2556 1842 1455 627 475 325 320 217 162 95 24 6 BanII G_rGCy'C Cuts at: 1391 2183 2509 4237 5423 1391 Size: 792 326 1728 1186 4072 Fragments arranged by size: 4072 1728 1186 792 326 BbeI G_GCGC'C Cuts at: 2385 4947 2385 Size: 2562 5542 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3003 3686 3993 928 Size: 971 1104 683 307 5039 Fragments arranged by size: 5039 1104 971 683 307 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6295 6329 1903 Size: 34 4358 34 3678 Fragments arranged by size: 4358 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5996 7206 5996 Size: 1210 6894 BclI T'GATC_A Cuts at: 1548 2466 2920 3723 4225 1548 Size: 918 454 803 502 5427 Fragments arranged by size: 5427 918 803 502 454 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2187 3971 6715 Size: 107 122 71 631 605 515 1784 2744 Cuts at: 6715 136 Size: 1525 Fragments arranged by size: 2744 1784 1525 631 605 515 122 107 71 BglII A'GATC_T Cuts at: 2728 8099 2728 Size: 5371 2733 BlpI GC'TnA_GC Cuts at: 5521 5521 Size: 8104 Bme1580I G_kGCm'C Cuts at: 1790 2183 3378 3976 4128 4237 5039 6165 7321 Size: 393 1195 598 152 109 802 1126 1156 Cuts at: 7321 1790 Size: 2573 Fragments arranged by size: 2573 1195 1156 1126 802 598 393 152 109 BmgBI CAC'GTC Cuts at: 1318 2269 5263 1318 Size: 951 2994 4159 Fragments arranged by size: 4159 2994 951 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2619 4231 4373 Size: 416 53 83 399 24 1202 1612 142 Cuts at: 4373 5196 5835 6793 442 Size: 823 639 958 1753 Fragments arranged by size: 1753 1612 1202 958 823 639 416 399 142 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8104 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2455 2455 Size: 8104 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 2339 2378 2810 3267 3378 3944 4340 Size: 1094 6 39 432 457 111 566 396 Cuts at: 4340 4433 6764 1239 Size: 93 2331 2579 Fragments arranged by size: 2579 2331 1094 566 457 432 396 111 93 39 6 Bpu10I CC'TnA_GC Cuts at: 2298 3980 4135 5141 2298 Size: 1682 155 1006 5261 Fragments arranged by size: 5261 1682 1006 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6229 7094 7392 6229 Size: 865 298 6941 Fragments arranged by size: 6941 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2520 3038 3552 4153 6767 882 Size: 617 1021 518 514 601 2614 2219 Fragments arranged by size: 2614 2219 1021 617 601 518 514 BsaAI yAC'GTr Cuts at: 493 1204 5895 493 Size: 711 4691 2702 Fragments arranged by size: 4691 2702 711 BsaBI GATnn'nnATC Cuts at: 8098 8098 Size: 8104 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2382 4944 5911 6293 Size: 53 83 186 936 849 2562 967 382 Cuts at: 6293 6928 275 Size: 635 1451 Fragments arranged by size: 2562 1451 967 936 849 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6537 6935 7209 7356 7767 6537 Size: 398 274 147 411 6874 Fragments arranged by size: 6874 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2695 2725 4900 4930 1178 Size: 30 165 30 1292 30 2175 30 4352 Fragments arranged by size: 4352 2175 1292 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2643 4900 1186 Size: 1457 2257 4390 Fragments arranged by size: 4390 2257 1457 BseYI C'CCAG_C Cuts at: 2418 3377 4767 4964 5056 5182 5291 7307 7803 Size: 959 1390 197 92 126 109 2016 496 Cuts at: 7803 2418 Size: 2719 Fragments arranged by size: 2719 2016 1390 959 496 197 126 109 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3405 4035 4403 5343 1163 Size: 573 1669 630 368 940 3924 Fragments arranged by size: 3924 1669 940 630 573 368 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2202 5554 6315 7343 1540 Size: 275 186 201 3352 761 1028 2301 Fragments arranged by size: 3352 2301 1028 761 275 201 186 BsiHKAI G_wGCw'C Cuts at: 2509 4616 4870 6165 6250 7321 2509 Size: 2107 254 1295 85 1071 3292 Fragments arranged by size: 3292 2107 1295 1071 254 85 BsmI GAATG_Cn' Cuts at: 5254 5615 5708 5254 Size: 361 93 7650 Fragments arranged by size: 7650 361 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2183 2509 3378 3976 4128 4237 4616 Size: 399 393 326 869 598 152 109 379 Cuts at: 4616 4870 5039 5423 6165 6250 7321 1391 Size: 254 169 384 742 85 1071 2174 Fragments arranged by size: 2174 1071 869 742 598 399 393 384 379 326 254 169 152 109 85 BspEI T'CCGG_A Cuts at: 7767 7767 Size: 8104 BspHI T'CATG_A Cuts at: 4008 4206 5993 7723 4008 Size: 198 1787 1730 4389 Fragments arranged by size: 4389 1787 1730 198 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3681 4378 4705 5459 844 Size: 2837 697 327 754 3489 Fragments arranged by size: 3489 2837 754 697 327 BsrBI CCG'CTC Cuts at: 1394 1823 2230 5245 5991 1394 Size: 429 407 3015 746 3507 Fragments arranged by size: 3507 3015 746 429 407 BsrDI GCAATG_nn' Cuts at: 65 1248 6599 6773 65 Size: 1183 5351 174 1396 Fragments arranged by size: 5351 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2156 2384 2390 3347 6748 6935 1700 Size: 110 346 228 6 957 3401 187 2869 Fragments arranged by size: 3401 2869 957 346 228 187 110 6 BsrGI T'GTAC_A Cuts at: 96 4602 96 Size: 4506 3598 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8104 BssSI C'ACGA_G Cuts at: 6164 7176 6164 Size: 1012 7092 BstAPI GCAn_nnn'nTGC Cuts at: 794 3242 4900 5080 794 Size: 2448 1658 180 3818 Fragments arranged by size: 3818 2448 1658 180 BstBI TT'CG_AA Cuts at: 2581 6908 2581 Size: 4327 3777 BstEII G'GTnAC_C Cuts at: 1184 5396 1184 Size: 4212 3892 BstXI CCAn_nnnn'nTGG Cuts at: 2537 4520 2537 Size: 1983 6121 BstYI r'GATC_y Cuts at: 1972 2728 3119 5434 5801 6186 6203 7655 7741 Size: 756 391 2315 367 385 17 1452 86 Cuts at: 7741 7753 8099 1972 Size: 12 346 1977 Fragments arranged by size: 2315 1977 1452 756 391 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2967 3553 5256 2967 Size: 586 1703 5815 Fragments arranged by size: 5815 1703 586 BtgI C'CryG_G Cuts at: 513 1065 2004 2220 3114 3171 4038 4063 5064 Size: 552 939 216 894 57 867 25 1001 Cuts at: 5064 513 Size: 3553 Fragments arranged by size: 3553 1001 939 894 867 552 216 57 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5070 487 Size: 683 56 3844 3521 Fragments arranged by size: 3844 3521 683 56 BtsI GCAGTG_nn' Cuts at: 2636 2788 3342 5620 6414 6434 2636 Size: 152 554 2278 794 20 4306 Fragments arranged by size: 4306 2278 794 554 152 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8104 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5260 5295 529 Size: 35 4696 35 3338 Fragments arranged by size: 4696 3338 35 35 DraI TTT'AAA Cuts at: 5408 5408 Size: 8104 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7111 809 Size: 507 5795 1802 Fragments arranged by size: 5795 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2952 3174 Size: 54 1425 50 275 18 369 753 222 Cuts at: 3174 3345 3852 4530 5551 6440 8069 8 Size: 171 507 678 1021 889 1629 43 Fragments arranged by size: 1629 1425 1021 889 753 678 507 369 275 222 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2199 5551 1537 Size: 275 387 3352 4090 Fragments arranged by size: 4090 3352 387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2661 3000 3071 4962 6034 1353 Size: 1308 339 71 1891 1072 3423 Fragments arranged by size: 3423 1891 1308 1072 339 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2225 2459 4328 6691 7065 7211 Size: 1064 138 636 234 1869 2363 374 146 Cuts at: 7211 387 Size: 1280 Fragments arranged by size: 2363 1869 1280 1064 636 374 234 146 138 EcoICRI GAG'CTC Cuts at: 2507 2507 Size: 8104 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2339 2378 2628 2810 3267 3378 Size: 393 701 6 39 250 182 457 111 Cuts at: 3378 3944 4340 4433 6167 6764 7551 7819 1239 Size: 566 396 93 1734 597 787 268 1524 Fragments arranged by size: 1734 1524 787 701 597 566 457 396 393 268 250 182 111 93 39 6 EcoNI CCTnn'n_nnAGG Cuts at: 4810 5144 4810 Size: 334 7770 EcoO109I rG'GnC_Cy Cuts at: 2179 2180 2983 3362 3557 3964 4106 4169 4505 Size: 1 803 379 195 407 142 63 336 Cuts at: 4505 4815 5046 2179 Size: 310 231 5237 Fragments arranged by size: 5237 803 407 379 336 310 231 195 142 63 1 EcoRI G'AATT_C Cuts at: 2577 3023 5413 5791 2577 Size: 446 2390 378 4890 Fragments arranged by size: 4890 2390 446 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5008 5008 Size: 8104 FspI TGC'GCA Cuts at: 4545 6610 4545 Size: 2065 6039 HaeII r_GCGC'y Cuts at: 1678 2326 2385 4947 7251 1678 Size: 648 59 2562 2304 2531 Fragments arranged by size: 2562 2531 2304 648 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2128 2160 6745 6777 6819 Size: 32 135 32 556 32 4585 32 42 Cuts at: 6819 6851 1373 Size: 32 2626 Fragments arranged by size: 4585 2626 556 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2272 2773 5448 5694 669 Size: 988 281 334 501 2675 246 3079 Fragments arranged by size: 3079 2675 988 501 334 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8104 HpaI GTT'AAC Cuts at: 5694 5694 Size: 8104 KasI G'GCGC_C Cuts at: 2381 4943 2381 Size: 2562 5542 KpnI G_GTAC'C Cuts at: 2290 5429 2290 Size: 3139 4965 MfeI C'AATT_G Cuts at: 5703 5703 Size: 8104 MluI A'CGCG_T Cuts at: 7980 7980 Size: 8104 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2478 2588 4066 4530 4696 7218 7402 1305 Size: 1173 110 1478 464 166 2522 184 2007 Fragments arranged by size: 2522 2007 1478 1173 464 184 166 110 MscI TGG'CCA Cuts at: 10 64 1489 1832 2954 3854 4532 10 Size: 54 1425 343 1122 900 678 3582 Fragments arranged by size: 3582 1425 1122 900 678 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 3719 3816 4619 4748 6062 6421 Size: 648 1056 1497 97 803 129 1314 359 Cuts at: 6421 6580 518 Size: 159 2042 Fragments arranged by size: 2042 1497 1314 1056 803 648 359 159 129 97 MspA1I CmG'CkG Cuts at: 1606 2027 2129 2311 2350 4231 4366 4541 5060 Size: 421 102 182 39 1881 135 175 519 Cuts at: 5060 5520 6197 7345 7590 1606 Size: 460 677 1148 245 2120 Fragments arranged by size: 2120 1881 1148 677 519 460 421 245 182 175 135 102 39 NaeI GCC'GGC Cuts at: 2386 2386 Size: 8104 NarI GG'CG_CC Cuts at: 2382 4944 2382 Size: 2562 5542 NcoI C'CATG_G Cuts at: 513 1065 2004 3114 4038 5064 513 Size: 552 939 1110 924 1026 3553 Fragments arranged by size: 3553 1110 1026 939 924 552 NdeI CA'TA_TG Cuts at: 387 3574 387 Size: 3187 4917 NgoMIV G'CCGG_C Cuts at: 2384 2384 Size: 8104 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8104 NotI GC'GGCC_GC Cuts at: 5551 5551 Size: 8104 NspI r_CATG'y Cuts at: 1132 1229 2635 3110 3391 4459 4747 4903 5164 Size: 97 1406 475 281 1068 288 156 261 Cuts at: 5164 5462 7007 1132 Size: 298 1545 2229 Fragments arranged by size: 2229 1545 1406 1068 475 298 288 281 261 156 97 PasI CC'CwG_GG Cuts at: 1348 3969 4520 4681 1348 Size: 2621 551 161 4771 Fragments arranged by size: 4771 2621 551 161 PciI A'CATG_T Cuts at: 3106 3387 7003 3106 Size: 281 3616 4207 Fragments arranged by size: 4207 3616 281 PflMI CCAn_nnn'nTGG Cuts at: 1108 2522 1108 Size: 1414 6690 PfoI T'CCnGG_A Cuts at: 5313 5313 Size: 8104 PmeI GTTT'AAAC Cuts at: 5408 5408 Size: 8104 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2695 2727 6173 6205 7686 7718 2695 Size: 32 3446 32 1481 32 3081 Fragments arranged by size: 3446 3081 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2983 3557 4505 5046 2983 Size: 574 948 541 6041 Fragments arranged by size: 6041 948 574 541 PshAI GACnn'nnGTC Cuts at: 1613 3828 1613 Size: 2215 5889 PsiI TTA'TAA Cuts at: 5674 5674 Size: 8104 PspOMI G'GGCC_C Cuts at: 2179 4233 2179 Size: 2054 6050 PstI C_TGCA'G Cuts at: 830 3595 3678 4839 5456 830 Size: 2765 83 1161 617 3478 Fragments arranged by size: 3478 2765 1161 617 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8104 PvuII CAG'CTG Cuts at: 2311 4541 5060 2311 Size: 2230 519 5355 Fragments arranged by size: 5355 2230 519 SacI G_AGCT'C Cuts at: 2509 2509 Size: 8104 SalI G'TCGA_C Cuts at: 1936 5446 1936 Size: 3510 4594 SapI GCTCTTCn'nnn_ Cuts at: 3071 3071 Size: 8104 SbfI CC_TGCA'GG Cuts at: 5456 5456 Size: 8104 ScaI AGT'ACT Cuts at: 2122 6352 8064 2122 Size: 4230 1712 2162 Fragments arranged by size: 4230 2162 1712 SfcI C'TryA_G Cuts at: 826 1045 3591 3648 3674 4835 5452 6587 7268 Size: 219 2546 57 26 1161 617 1135 681 Cuts at: 7268 7459 826 Size: 191 1471 Fragments arranged by size: 2546 1471 1161 1135 681 617 219 191 57 26 SfoI GGC'GCC Cuts at: 2383 4945 2383 Size: 2562 5542 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8104 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8104 SmaI CCC'GGG Cuts at: 1872 5094 5431 1872 Size: 3222 337 4545 Fragments arranged by size: 4545 3222 337 SmlI C'TyrA_G Cuts at: 820 1017 1943 6208 7109 7371 820 Size: 197 926 4265 901 262 1553 Fragments arranged by size: 4265 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5895 493 Size: 5402 2702 SpeI A'CTAG_T Cuts at: 152 3179 4913 152 Size: 3027 1734 3343 Fragments arranged by size: 3343 3027 1734 SphI G_CATG'C Cuts at: 1132 4459 4747 4903 5164 5462 1132 Size: 3327 288 156 261 298 3774 Fragments arranged by size: 3774 3327 298 288 261 156 SspI AAT'ATT Cuts at: 5 52 3778 6028 5 Size: 47 3726 2250 2081 Fragments arranged by size: 3726 2250 2081 47 StuI AGG'CCT Cuts at: 1517 3136 4805 1517 Size: 1619 1669 4816 Fragments arranged by size: 4816 1669 1619 StyI C'CwwG_G Cuts at: 513 1065 2004 3114 4038 4811 4829 5064 5543 Size: 552 939 1110 924 773 18 235 479 Cuts at: 5543 513 Size: 3074 Fragments arranged by size: 3074 1110 939 924 773 552 479 235 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4702 5196 6131 6148 6301 6486 6931 Size: 1255 3122 494 935 17 153 185 445 Cuts at: 6931 325 Size: 1498 Fragments arranged by size: 3122 1498 1255 935 494 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2120 4602 6350 Size: 274 80 33 51 1443 143 2482 1748 Cuts at: 6350 8062 96 Size: 1712 138 Fragments arranged by size: 2482 1748 1712 1443 274 143 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4900 4932 8064 8096 4900 Size: 32 3132 32 4908 Fragments arranged by size: 4908 3132 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8104 XbaI T'CTAG_A Cuts at: 2722 5440 2722 Size: 2718 5386 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5364 5364 Size: 8104 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8104 XmaI C'CCGG_G Cuts at: 1870 5092 5429 1870 Size: 3222 337 4545 Fragments arranged by size: 4545 3222 337 XmnI GAAnn'nnTTC Cuts at: 6233 6233 Size: 8104 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5912 276 Size: 53 83 186 936 4378 2468 Fragments arranged by size: 4378 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI