Restriction map for
pFN21AA1816
(Linear) (Six-base) MAPSORT of: KIAA1816.seq Check: 6391 from: 1 to: 3399
KIAA1816.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
Acc65I G'GTAC_C
Cuts at: 0 281 3399
Size: 281 3118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 3399
Size: 623 2776
AflIII A'CryG_T
Cuts at: 0 1101 1382 3257 3399
Size: 1101 281 1875 142
Fragments arranged by size:
1875 1101 281 142
AleI CACnn'nnGTG
Cuts at: 0 217 3399
Size: 217 3182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 551 3399
Size: 551 2848
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 690 722 3399
Size: 690 32 2677
Fragments arranged by size:
2677 690 32
AlwNI CAG_nnn'CTG
Cuts at: 0 981 1036 1360 2262 3000 3399
Size: 981 55 324 902 738 399
Fragments arranged by size:
981 902 738 399 324 55
ApaI G_GGCC'C
Cuts at: 0 178 2232 3399
Size: 178 2054 1167
Fragments arranged by size:
2054 1167 178
ApoI r'AATT_y
Cuts at: 0 549 572 1018 3399
Size: 549 23 446 2381
Fragments arranged by size:
2381 549 446 23
AseI AT'TA_AT
Cuts at: 0 905 934 3399
Size: 905 29 2465
Fragments arranged by size:
2465 905 29
AvaI C'yCGr_G
Cuts at: 0 402 3087 3399
Size: 402 2685 312
Fragments arranged by size:
2685 402 312
AvrII C'CTAG_G
Cuts at: 0 2806 3399
Size: 2806 593
BanI G'GyrC_C
Cuts at: 0 257 281 376 382 2938 3100 3399
Size: 257 24 95 6 2556 162 299
Fragments arranged by size:
2556 299 257 162 95 24 6
BanII G_rGCy'C
Cuts at: 0 178 504 2232 3399
Size: 178 326 1728 1167
Fragments arranged by size:
1728 1167 326 178
BbeI G_GCGC'C
Cuts at: 0 380 2942 3399
Size: 380 2562 457
Fragments arranged by size:
2562 457 380
BbsI GAAGACnn'nnnn_
Cuts at: 0 998 1681 1988 3399
Size: 998 683 307 1411
Fragments arranged by size:
1411 998 683 307
BclI T'GATC_A
Cuts at: 0 461 915 1718 2220 3399
Size: 461 454 803 502 1179
Fragments arranged by size:
1179 803 502 461 454
BglI GCCn_nnn'nGGC
Cuts at: 0 182 1966 3399
Size: 182 1784 1433
Fragments arranged by size:
1784 1433 182
BglII A'GATC_T
Cuts at: 0 723 3399
Size: 723 2676
Bme1580I G_kGCm'C
Cuts at: 0 178 1373 1971 2123 2232 3034 3399
Size: 178 1195 598 152 109 802 365
Fragments arranged by size:
1195 802 598 365 178 152 109
BmgBI CAC'GTC
Cuts at: 0 264 3258 3399
Size: 264 2994 141
Fragments arranged by size:
2994 264 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 614 2226 2368 3191 3399
Size: 614 1612 142 823 208
Fragments arranged by size:
1612 823 614 208 142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 450 3399
Size: 450 2949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 334 373 805 1262 1373 1939 2335
Size: 328 6 39 432 457 111 566 396
Cuts at: 2335 2428 3399
Size: 93 971
Fragments arranged by size:
971 566 457 432 396 328 111 93
39 6
Bpu10I CC'TnA_GC
Cuts at: 0 293 1975 2130 3136 3399
Size: 293 1682 155 1006 263
Fragments arranged by size:
1682 1006 293 263 155
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 1033 1547 2148 3399
Size: 515 518 514 601 1251
Fragments arranged by size:
1251 601 518 515 514
BsaHI Gr'CG_yC
Cuts at: 0 377 2939 3399
Size: 377 2562 460
Fragments arranged by size:
2562 460 377
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 690 720 2895 2925 3399
Size: 690 30 2175 30 474
Fragments arranged by size:
2175 690 474 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 638 2895 3399
Size: 638 2257 504
Fragments arranged by size:
2257 638 504
BseYI C'CCAG_C
Cuts at: 0 413 1372 2762 2959 3051 3177 3286 3399
Size: 413 959 1390 197 92 126 109 113
Fragments arranged by size:
1390 959 413 197 126 113 109 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1400 2030 2398 3338 3399
Size: 1400 630 368 940 61
Fragments arranged by size:
1400 940 630 368 61
BsiEI CG_ry'CG
Cuts at: 0 197 3399
Size: 197 3202
BsiHKAI G_wGCw'C
Cuts at: 0 504 2611 2865 3399
Size: 504 2107 254 534
Fragments arranged by size:
2107 534 504 254
BsmI GAATG_Cn'
Cuts at: 0 3249 3399
Size: 3249 150
Bsp1286I G_dGCh'C
Cuts at: 0 178 504 1373 1971 2123 2232 2611 2865
Size: 178 326 869 598 152 109 379 254
Cuts at: 2865 3034 3399
Size: 169 365
Fragments arranged by size:
869 598 379 365 326 254 178 169
152 109
BspHI T'CATG_A
Cuts at: 0 2003 2201 3399
Size: 2003 198 1198
Fragments arranged by size:
2003 1198 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1676 2373 2700 3399
Size: 1676 697 327 699
Fragments arranged by size:
1676 699 697 327
BsrBI CCG'CTC
Cuts at: 0 225 3240 3399
Size: 225 3015 159
Fragments arranged by size:
3015 225 159
BsrFI r'CCGG_y
Cuts at: 0 151 379 385 1342 3399
Size: 151 228 6 957 2057
Fragments arranged by size:
2057 957 228 151 6
BsrGI T'GTAC_A
Cuts at: 0 2597 3399
Size: 2597 802
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1237 2895 3075 3399
Size: 1237 1658 180 324
Fragments arranged by size:
1658 1237 324 180
BstBI TT'CG_AA
Cuts at: 0 576 3399
Size: 576 2823
BstEII G'GTnAC_C
Cuts at: 0 3391 3399
Size: 3391 8
BstXI CCAn_nnnn'nTGG
Cuts at: 0 532 2515 3399
Size: 532 1983 884
Fragments arranged by size:
1983 884 532
BstYI r'GATC_y
Cuts at: 0 723 1114 3399
Size: 723 391 2285
Fragments arranged by size:
2285 723 391
Bsu36I CC'TnA_GG
Cuts at: 0 962 1548 3251 3399
Size: 962 586 1703 148
Fragments arranged by size:
1703 962 586 148
BtgI C'CryG_G
Cuts at: 0 215 1109 1166 2033 2058 3059 3399
Size: 215 894 57 867 25 1001 340
Fragments arranged by size:
1001 894 867 340 215 57 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3065 3399
Size: 3065 334
BtsI GCAGTG_nn'
Cuts at: 0 631 783 1337 3399
Size: 631 152 554 2062
Fragments arranged by size:
2062 631 554 152
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3255 3290 3399
Size: 3255 35 109
Fragments arranged by size:
3255 109 35
EaeI y'GGCC_r
Cuts at: 0 194 947 1169 1340 1847 2525 3399
Size: 194 753 222 171 507 678 874
Fragments arranged by size:
874 753 678 507 222 194 171
EagI C'GGCC_G
Cuts at: 0 194 3399
Size: 194 3205
EarI CTCTTCn'nnn_
Cuts at: 0 656 995 1066 2957 3399
Size: 656 339 71 1891 442
Fragments arranged by size:
1891 656 442 339 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 220 454 2323 3399
Size: 220 234 1869 1076
Fragments arranged by size:
1869 1076 234 220
EcoICRI GAG'CTC
Cuts at: 0 502 3399
Size: 502 2897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 334 373 623 805 1262 1373 1939
Size: 328 6 39 250 182 457 111 566
Cuts at: 1939 2335 2428 3399
Size: 396 93 971
Fragments arranged by size:
971 566 457 396 328 250 182 111
93 39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2805 3139 3399
Size: 2805 334 260
Fragments arranged by size:
2805 334 260
EcoO109I rG'GnC_Cy
Cuts at: 0 174 175 978 1357 1552 1959 2101 2164
Size: 174 1 803 379 195 407 142 63
Cuts at: 2164 2500 2810 3041 3399
Size: 336 310 231 358
Fragments arranged by size:
803 407 379 358 336 310 231 195
174 142 63 1
EcoRI G'AATT_C
Cuts at: 0 572 1018 3399
Size: 572 446 2381
Fragments arranged by size:
2381 572 446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3003 3399
Size: 3003 396
FspI TGC'GCA
Cuts at: 0 2540 3399
Size: 2540 859
HaeII r_GCGC'y
Cuts at: 0 321 380 2942 3399
Size: 321 59 2562 457
Fragments arranged by size:
2562 457 321 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 123 155 3399
Size: 123 32 3244
Fragments arranged by size:
3244 123 32
HincII GTy'rAC
Cuts at: 0 267 768 3399
Size: 267 501 2631
Fragments arranged by size:
2631 501 267
KasI G'GCGC_C
Cuts at: 0 376 2938 3399
Size: 376 2562 461
Fragments arranged by size:
2562 461 376
KpnI G_GTAC'C
Cuts at: 0 285 3399
Size: 285 3114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 583 2061 2525 2691 3399
Size: 473 110 1478 464 166 708
Fragments arranged by size:
1478 708 473 464 166 110
MscI TGG'CCA
Cuts at: 0 949 1849 2527 3399
Size: 949 900 678 872
Fragments arranged by size:
949 900 872 678
MslI CAynn'nnrTG
Cuts at: 0 217 1714 1811 2614 2743 3399
Size: 217 1497 97 803 129 656
Fragments arranged by size:
1497 803 656 217 129 97
MspA1I CmG'CkG
Cuts at: 0 22 124 306 345 2226 2361 2536 3055
Size: 22 102 182 39 1881 135 175 519
Cuts at: 3055 3399
Size: 344
Fragments arranged by size:
1881 519 344 182 175 135 102 39
22
NaeI GCC'GGC
Cuts at: 0 381 3399
Size: 381 3018
NarI GG'CG_CC
Cuts at: 0 377 2939 3399
Size: 377 2562 460
Fragments arranged by size:
2562 460 377
NcoI C'CATG_G
Cuts at: 0 1109 2033 3059 3399
Size: 1109 924 1026 340
Fragments arranged by size:
1109 1026 924 340
NdeI CA'TA_TG
Cuts at: 0 1569 3399
Size: 1569 1830
NgoMIV G'CCGG_C
Cuts at: 0 379 3399
Size: 379 3020
NspI r_CATG'y
Cuts at: 0 630 1105 1386 2454 2742 2898 3159 3399
Size: 630 475 281 1068 288 156 261 240
Fragments arranged by size:
1068 630 475 288 281 261 240 156
PasI CC'CwG_GG
Cuts at: 0 1964 2515 2676 3399
Size: 1964 551 161 723
Fragments arranged by size:
1964 723 551 161
PciI A'CATG_T
Cuts at: 0 1101 1382 3399
Size: 1101 281 2017
Fragments arranged by size:
2017 1101 281
PflMI CCAn_nnn'nTGG
Cuts at: 0 517 3399
Size: 517 2882
PfoI T'CCnGG_A
Cuts at: 0 3308 3399
Size: 3308 91
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 690 722 3399
Size: 690 32 2677
Fragments arranged by size:
2677 690 32
PpuMI rG'GwC_Cy
Cuts at: 0 978 1552 2500 3041 3399
Size: 978 574 948 541 358
Fragments arranged by size:
978 948 574 541 358
PshAI GACnn'nnGTC
Cuts at: 0 1823 3399
Size: 1823 1576
PspOMI G'GGCC_C
Cuts at: 0 174 2228 3399
Size: 174 2054 1171
Fragments arranged by size:
2054 1171 174
PstI C_TGCA'G
Cuts at: 0 1590 1673 2834 3399
Size: 1590 83 1161 565
Fragments arranged by size:
1590 1161 565 83
PvuII CAG'CTG
Cuts at: 0 306 2536 3055 3399
Size: 306 2230 519 344
Fragments arranged by size:
2230 519 344 306
SacI G_AGCT'C
Cuts at: 0 504 3399
Size: 504 2895
SapI GCTCTTCn'nnn_
Cuts at: 0 1066 3399
Size: 1066 2333
ScaI AGT'ACT
Cuts at: 0 117 3399
Size: 117 3282
SfcI C'TryA_G
Cuts at: 0 1586 1643 1669 2830 3399
Size: 1586 57 26 1161 569
Fragments arranged by size:
1586 1161 569 57 26
SfoI GGC'GCC
Cuts at: 0 378 2940 3399
Size: 378 2562 459
Fragments arranged by size:
2562 459 378
SmaI CCC'GGG
Cuts at: 0 3089 3399
Size: 3089 310
SpeI A'CTAG_T
Cuts at: 0 1174 2908 3399
Size: 1174 1734 491
Fragments arranged by size:
1734 1174 491
SphI G_CATG'C
Cuts at: 0 2454 2742 2898 3159 3399
Size: 2454 288 156 261 240
Fragments arranged by size:
2454 288 261 240 156
SspI AAT'ATT
Cuts at: 0 1773 3399
Size: 1773 1626
StuI AGG'CCT
Cuts at: 0 1131 2800 3399
Size: 1131 1669 599
Fragments arranged by size:
1669 1131 599
StyI C'CwwG_G
Cuts at: 0 1109 2033 2806 2824 3059 3399
Size: 1109 924 773 18 235 340
Fragments arranged by size:
1109 924 773 340 235 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2697 3191 3399
Size: 2697 494 208
Fragments arranged by size:
2697 494 208
TatI w'GTAC_w
Cuts at: 0 115 2597 3399
Size: 115 2482 802
Fragments arranged by size:
2482 802 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2895 2927 3399
Size: 2895 32 472
Fragments arranged by size:
2895 472 32
XbaI T'CTAG_A
Cuts at: 0 717 3399
Size: 717 2682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3359 3399
Size: 3359 40
XmaI C'CCGG_G
Cuts at: 0 3087 3399
Size: 3087 312
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlfI AloI AlwNI ApaI
ApoI AseI AvaI AvrII BanI BanII BbeI BbsI
BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SapI
ScaI SfcI SfoI SmaI SpeI SphI SspI StuI
StyI TaqII TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
ApaLI AscI AsiSI BaeI BamHI BbvCI BcgI BciVI
BfrBI BlpI BmtI BpuEI BsaAI BsaBI BsaWI BsiWI
BsmBI BspEI BsrDI BssHII BssSI BstZ17I ClaI DraI
DraIII DrdI EcoRV FseI FspAI HindIII HpaI MfeI
MluI NheI NotI NruI NsiI PacI PmeI PmlI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmlI SnaBI SrfI
SwaI Tth111I XhoI XmnI ZraI