(Circular) (Six-base) MAPSORT of: pFN21AA1680.seq Check: 7931 from: 1 to: 7405 pFN21AA1680 7405 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5215 278 Size: 53 83 186 936 3679 2468 Fragments arranged by size: 3679 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4748 1937 Size: 2811 4594 Acc65I G'GTAC_C Cuts at: 4726 4726 Size: 7405 AclI AA'CG_TT Cuts at: 1563 5532 5905 1563 Size: 3969 373 3063 Fragments arranged by size: 3969 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2760 2817 4014 4070 4406 4431 4514 5468 Size: 1128 57 1197 56 336 25 83 954 Cuts at: 5468 6852 7120 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1197 1128 954 336 268 83 57 56 25 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7208 AflIII A'CryG_T Cuts at: 1203 3220 4626 6304 7281 1203 Size: 2017 1406 1678 977 1327 Fragments arranged by size: 2017 1678 1406 1327 977 AgeI A'CCGG_T Cuts at: 6236 6236 Size: 7405 AhdI GACnn_n'nnGTC Cuts at: 6134 6134 Size: 7405 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7405 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4694 1184 Size: 3510 3895 AlwNI CAG_nnn'CTG Cuts at: 2311 5148 6720 2311 Size: 2837 1572 2996 Fragments arranged by size: 2996 2837 1572 ApaLI G'TGCA_C Cuts at: 5462 6618 5462 Size: 1156 6249 ApoI r'AATT_y Cuts at: 1493 2573 2627 2780 3575 3707 4714 4940 5092 Size: 1080 54 153 795 132 1007 226 152 Cuts at: 5092 7201 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1080 1007 795 226 153 152 132 54 AseI AT'TA_AT Cuts at: 160 1032 5959 160 Size: 872 4927 1606 Fragments arranged by size: 4927 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7405 AvaI C'yCGr_G Cuts at: 1870 1943 4730 1870 Size: 73 2787 4545 Fragments arranged by size: 4545 2787 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2606 2639 2606 Size: 33 7372 BamHI G'GATC_C Cuts at: 3231 4735 5102 3231 Size: 1504 367 5534 Fragments arranged by size: 5534 1504 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4726 6181 618 Size: 325 217 627 2939 1455 1842 Fragments arranged by size: 2939 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4490 4521 4724 1391 Size: 3099 31 203 4072 Fragments arranged by size: 4072 3099 203 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2083 2350 2508 3587 928 Size: 971 184 267 158 1079 4746 Fragments arranged by size: 4746 1079 971 267 184 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5596 5630 1903 Size: 34 3659 34 3678 Fragments arranged by size: 3678 3659 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5297 6507 5297 Size: 1210 6195 BclI T'GATC_A Cuts at: 1548 3274 3760 1548 Size: 1726 486 5193 Fragments arranged by size: 5193 1726 486 BfrBI ATG'CAT Cuts at: 2822 2822 Size: 7405 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6016 136 Size: 107 122 71 631 605 4344 1525 Fragments arranged by size: 4344 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2390 7400 2390 Size: 5010 2395 BlpI GC'TnA_GC Cuts at: 4822 4822 Size: 7405 Bme1580I G_kGCm'C Cuts at: 1790 5466 6622 1790 Size: 3676 1156 2573 Fragments arranged by size: 3676 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3969 1318 Size: 2651 4754 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5136 6094 442 Size: 416 53 83 399 24 3719 958 1753 Fragments arranged by size: 3719 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7405 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6065 1239 Size: 4826 2579 Bpu10I CC'TnA_GC Cuts at: 2161 2238 3714 2161 Size: 77 1476 5852 Fragments arranged by size: 5852 1476 77 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2140 4028 5530 6395 6693 2140 Size: 1888 1502 865 298 2852 Fragments arranged by size: 2852 1888 1502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2044 6068 882 Size: 617 545 4024 2219 Fragments arranged by size: 4024 2219 617 545 BsaAI yAC'GTr Cuts at: 493 1204 5196 493 Size: 711 3992 2702 Fragments arranged by size: 3992 2702 711 BsaBI GATnn'nnATC Cuts at: 7399 7399 Size: 7405 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5212 5594 6229 275 Size: 53 83 186 936 3679 382 635 1451 Fragments arranged by size: 3679 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3016 5838 6236 6510 6657 7068 3016 Size: 2822 398 274 147 411 3353 Fragments arranged by size: 3353 2822 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7180 Fragments arranged by size: 7180 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2197 3391 1186 Size: 1011 1194 5200 Fragments arranged by size: 5200 1194 1011 BseYI C'CCAG_C Cuts at: 3160 4096 6608 7104 3160 Size: 936 2512 496 3461 Fragments arranged by size: 3461 2512 936 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3871 3960 1163 Size: 573 2135 89 4608 Fragments arranged by size: 4608 2135 573 89 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4855 5616 6644 1540 Size: 275 186 2854 761 1028 2301 Fragments arranged by size: 2854 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2284 2427 4490 5466 5551 6622 2284 Size: 143 2063 976 85 1071 3067 Fragments arranged by size: 3067 2063 1071 976 143 85 BsmI GAATG_Cn' Cuts at: 3798 4037 4570 4916 5009 3798 Size: 239 533 346 93 6194 Fragments arranged by size: 6194 533 346 239 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3643 3643 Size: 7405 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2284 2427 4490 4521 4724 5466 5551 Size: 399 494 143 2063 31 203 742 85 Cuts at: 5551 6622 1391 Size: 1071 2174 Fragments arranged by size: 2174 2063 1071 742 494 399 203 143 85 31 BspEI T'CCGG_A Cuts at: 7068 7068 Size: 7405 BspHI T'CATG_A Cuts at: 5294 7024 5294 Size: 1730 5675 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3079 3473 4578 4760 844 Size: 2235 394 1105 182 3489 Fragments arranged by size: 3489 2235 1105 394 182 BsrBI CCG'CTC Cuts at: 1394 1823 5292 1394 Size: 429 3469 3507 Fragments arranged by size: 3507 3469 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5900 6074 65 Size: 1183 4652 174 1396 Fragments arranged by size: 4652 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6049 6236 1700 Size: 110 4239 187 2869 Fragments arranged by size: 4239 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4629 96 Size: 4533 2872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7405 BssSI C'ACGA_G Cuts at: 5465 6477 5465 Size: 1012 6393 BstAPI GCAn_nnn'nTGC Cuts at: 794 3320 794 Size: 2526 4879 BstBI TT'CG_AA Cuts at: 6209 6209 Size: 7405 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7405 BstXI CCAn_nnnn'nTGG Cuts at: 2611 4700 2611 Size: 2089 5316 BstYI r'GATC_y Cuts at: 1972 2390 3231 3533 4735 5102 5487 5504 6956 Size: 418 841 302 1202 367 385 17 1452 Cuts at: 6956 7042 7054 7400 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1202 841 418 385 367 346 302 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5914 Fragments arranged by size: 5914 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6666 Fragments arranged by size: 6666 683 56 BtsI GCAGTG_nn' Cuts at: 3402 4921 5715 5735 3402 Size: 1519 794 20 5072 Fragments arranged by size: 5072 1519 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7405 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7370 DraI TTT'AAA Cuts at: 4709 4709 Size: 7405 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6412 809 Size: 507 5096 1802 Fragments arranged by size: 5096 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2866 4852 5741 Size: 54 1425 50 275 18 1036 1986 889 Cuts at: 5741 7370 8 Size: 1629 43 Fragments arranged by size: 1986 1629 1425 1036 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4852 1537 Size: 275 3040 4090 Fragments arranged by size: 4090 3040 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 3914 3990 4086 5335 1353 Size: 741 1820 76 96 1249 3423 Fragments arranged by size: 3423 1820 1249 741 96 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5992 6366 6512 387 Size: 1064 138 4403 374 146 1280 Fragments arranged by size: 4403 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4488 4488 Size: 7405 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2760 2817 4014 4070 4406 4431 4514 Size: 393 1128 57 1197 56 336 25 83 Cuts at: 4514 5468 6065 6852 7120 1239 Size: 954 597 787 268 1524 Fragments arranged by size: 1524 1197 1128 954 787 597 393 336 268 83 57 56 25 EcoNI CCTnn'n_nnAGG Cuts at: 3825 3825 Size: 7405 EcoRI G'AATT_C Cuts at: 2627 4714 5092 2627 Size: 2087 378 4940 Fragments arranged by size: 4940 2087 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 4446 2461 Size: 1985 5420 FspI TGC'GCA Cuts at: 5911 5911 Size: 7405 HaeII r_GCGC'y Cuts at: 1678 6552 1678 Size: 4874 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2792 2824 2867 Size: 32 135 32 431 32 757 32 43 Cuts at: 2867 2899 4019 4051 4237 4269 4599 4631 6046 Size: 32 1120 32 186 32 330 32 1415 Cuts at: 6046 6078 6120 6152 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1415 1120 757 431 330 186 135 43 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2532 4355 4749 4995 669 Size: 988 281 594 1823 394 246 3079 Fragments arranged by size: 3079 1823 988 594 394 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7405 HpaI GTT'AAC Cuts at: 4995 4995 Size: 7405 KpnI G_GTAC'C Cuts at: 4730 4730 Size: 7405 MfeI C'AATT_G Cuts at: 5004 5004 Size: 7405 MluI A'CGCG_T Cuts at: 7281 7281 Size: 7405 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6519 6703 1305 Size: 5214 184 2007 Fragments arranged by size: 5214 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2868 10 Size: 54 1425 343 1036 4547 Fragments arranged by size: 4547 1425 1036 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3306 5363 5722 5881 518 Size: 648 2140 2057 359 159 2042 Fragments arranged by size: 2140 2057 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4054 4319 4821 5498 6646 6891 1606 Size: 2448 265 502 677 1148 245 2120 Fragments arranged by size: 2448 2120 1148 677 502 265 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5914 Fragments arranged by size: 5914 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7405 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7405 NotI GC'GGCC_GC Cuts at: 4852 4852 Size: 7405 NsiI A_TGCA'T Cuts at: 2824 2824 Size: 7405 NspI r_CATG'y Cuts at: 1132 1229 3224 4630 4763 6308 1132 Size: 97 1995 1406 133 1545 2229 Fragments arranged by size: 2229 1995 1545 1406 133 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7405 PciI A'CATG_T Cuts at: 3220 4626 6304 3220 Size: 1406 1678 4321 Fragments arranged by size: 4321 1678 1406 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7405 PmeI GTTT'AAAC Cuts at: 4709 4709 Size: 7405 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5474 5506 6987 7019 5474 Size: 32 1481 32 5860 Fragments arranged by size: 5860 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3948 1613 Size: 2335 5070 PsiI TTA'TAA Cuts at: 2450 4975 2450 Size: 2525 4880 PstI C_TGCA'G Cuts at: 830 3409 4053 4757 830 Size: 2579 644 704 3478 Fragments arranged by size: 3478 2579 704 644 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7405 PvuII CAG'CTG Cuts at: 4054 4054 Size: 7405 SacI G_AGCT'C Cuts at: 4490 4490 Size: 7405 SalI G'TCGA_C Cuts at: 1936 4747 1936 Size: 2811 4594 SapI GCTCTTCn'nnn_ Cuts at: 3990 4086 3990 Size: 96 7309 SbfI CC_TGCA'GG Cuts at: 4757 4757 Size: 7405 ScaI AGT'ACT Cuts at: 5653 7365 5653 Size: 1712 5693 SexAI A'CCwGG_T Cuts at: 2287 2287 Size: 7405 SfcI C'TryA_G Cuts at: 826 1045 2591 3405 4049 4753 5888 6569 6760 Size: 219 1546 814 644 704 1135 681 191 Cuts at: 6760 826 Size: 1471 Fragments arranged by size: 1546 1471 1135 814 704 681 644 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7405 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7405 SmaI CCC'GGG Cuts at: 1872 4732 1872 Size: 2860 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2155 4043 5509 6410 6672 820 Size: 197 926 212 1888 1466 901 262 1553 Fragments arranged by size: 1888 1553 1466 926 901 262 212 197 SnaBI TAC'GTA Cuts at: 493 5196 493 Size: 4703 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7405 SphI G_CATG'C Cuts at: 1132 4763 1132 Size: 3631 3774 SspI AAT'ATT Cuts at: 5 52 2055 5329 5 Size: 47 2003 3274 2081 Fragments arranged by size: 3274 2081 2003 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7405 StyI C'CwwG_G Cuts at: 513 1065 2004 3176 4844 513 Size: 552 939 1172 1668 3074 Fragments arranged by size: 3074 1668 1172 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4618 5432 5449 5602 5787 6232 325 Size: 1255 3038 814 17 153 185 445 1498 Fragments arranged by size: 3038 1498 1255 814 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4204 4629 5651 Size: 274 80 33 51 1443 2227 425 1022 Cuts at: 5651 7363 96 Size: 1712 138 Fragments arranged by size: 2227 1712 1443 1022 425 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3958 3990 7365 7397 3958 Size: 32 3375 32 3966 Fragments arranged by size: 3966 3375 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7405 XbaI T'CTAG_A Cuts at: 4741 4741 Size: 7405 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3813 3813 Size: 7405 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7405 XmaI C'CCGG_G Cuts at: 1870 4730 1870 Size: 2860 4545 XmnI GAAnn'nnTTC Cuts at: 2347 5534 2347 Size: 3187 4218 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5213 276 Size: 53 83 186 936 3679 2468 Fragments arranged by size: 3679 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI