Restriction map for
pFN21AA1668
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI AspEI AvrII
BcgI BfrI BglII BlnI BsaBI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI ScaI SfiI SfuI SgrAI
SnaBI SphI SspBI SspI SwaI
Tsp509I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1636 GGTACC G^GTACC
AcyI 1795 GGCGCC G[GA]^CG[CT]C
Alw44I 511 GTGCAC G^TGCAC
ApaLI 511 GTGCAC G^TGCAC
Asp700I 2466 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1636 GGTACC G^GTACC
AviII 490 TGCGCA TGC^GCA
BbeI 1795 GGCGCC GGCGC^C
BbrPI 486 CACGTG CAC^GTG
BciVI 2064 GGATAC GTATCC(6/5)
BclI 2456 TGATCA T^GATCA
BglI 1965 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1524 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1524 GCTCAGC GC^T[ACGT]AGC
BsaAI 486 CACGTG [CT]AC^GT[GA]
BsaHI 1795 GGCGCC G[GA]^CG[CT]C
BsaI 255 GGTCTC GGTCTC(1/5)
BspHI 287 TCATGA T^CATGA
BssSI 2188 CACGAG CACGAG(-5/-1)
CelII 1524 GCTCAGC GC^T[ACGT]AGC
DraIII 514 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 291 GACCTATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 829 CGGCCG C^GGCCG
EclXI 829 CGGCCG C^GGCCG
Eco52I 829 CGGCCG C^GGCCG
FbaI 2456 TGATCA T^GATCA
FspI 490 TGCGCA TGC^GCA
Hin1I 1795 GGCGCC G[GA]^CG[CT]C
KasI 1795 GGCGCC G^GCGCC
KpnI 1636 GGTACC GGTAC^C
MaeIII 2365 GTGAC ^GT[ACGT]AC
MseI 1904 TTAA T^TAA
NaeI 2078 GCCGGC GCC^GGC
NarI 1795 GGCGCC GG^CGCC
NgoMIV 2078 GCCGGC G^CCGGC
PmaCI 486 CACGTG CAC^GTG
PmlI 486 CACGTG CAC^GTG
RcaI 287 TCATGA T^CATGA
SbfI 784 CCTGCAGG CCTGCA^GG
SexAI 986 ACCTGGT A^CC[AT]GGT
SfoI 1795 GGCGCC GGC^GCC
SmlI 1724 CTCAAG C^T[CT][GA]AG
SpeI 1695 ACTAGT A^CTAGT
Sse8387I 784 CCTGCAGG CCTGCA^GG
Tru9I 1904 TTAA T^TAA
Tsp45I 2365 GTGAC ^GT[GC]AC
XmnI 2466 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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Aor51HI 524 AGCGCT AGC^GCT
1521 AGCGCT
BamHI 224 GGATCC G^GATCC
1957 GGATCC
BbvCI 279 CCTCAGC CCTCAGC(-5/-2)
793 GCTGAGG
BsiEI 676 CGGTCG CG[GA][CT]^CG
829 CGGCCG
BsrBI 230 CCGCTC CCGCTC(-3/-3)
963 CCGCTC
BssHII 17 GCGCGC G^CGCGC
1440 GCGCGC
EarI 1306 GAAGAG CTCTTC(1/4)
2428 GAAGAG
Eco47III 524 AGCGCT AGC^GCT
1521 AGCGCT
EcoNI 2136 CCTGAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2403 CCTGGATGAGG
Ksp632I 1306 GAAGAG CTCTTC(1/4)
2428 GAAGAG
KspI 10 CCGCGG CCGC^GG
57 CCGCGG
MaeII 487 ACGT A^CGT
2553 ACGT
NcoI 1108 CCATGG C^CATGG
2052 CCATGG
NspI 507 GCATGT [GA]CATG^[CT]
2159 ACATGC
PpuMI 1100 AGGACCC [GA]G^G[AT]CC[CT]
1767 AGGTCCC
SacII 10 CCGCGG CCGC^GG
57 CCGCGG
StuI 688 AGGCCT AGG^CCT
920 AGGCCT
TaqI 179 TCGA T^CGA
2273 TCGA
TfiI 154 GATTC G^A[AT]TC
1896 GAATC
TthHB8I 179 TCGA T^CGA
2273 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TseI TspRI Tth111I XcmI XhoII
XmaCI XmaI