(Circular) (Six-base) MAPSORT of: pFN21AA1668.seq Check: 2971 from: 1 to: 7294 pFN21AA1668 7294 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3196 3563 3934 3991 3196 Size: 367 371 57 6499 Fragments arranged by size: 6499 371 367 57 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5104 278 Size: 53 83 186 936 3568 2468 Fragments arranged by size: 3568 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4637 1937 Size: 2700 4594 Acc65I G'GTAC_C Cuts at: 3641 4615 3641 Size: 974 6320 AclI AA'CG_TT Cuts at: 1563 5421 5794 1563 Size: 3858 373 3063 Fragments arranged by size: 3858 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5357 6741 7009 1632 Size: 3725 1384 268 1917 Fragments arranged by size: 3725 1917 1384 268 AfeI AGC'GCT Cuts at: 2531 3528 2531 Size: 997 6297 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7097 AflIII A'CryG_T Cuts at: 1203 6193 7170 1203 Size: 4990 977 1327 Fragments arranged by size: 4990 1327 977 AgeI A'CCGG_T Cuts at: 6125 6125 Size: 7294 AhdI GACnn_n'nnGTC Cuts at: 6023 6023 Size: 7294 AleI CACnn'nnGTG Cuts at: 1166 2405 2639 3125 1166 Size: 1239 234 486 5335 Fragments arranged by size: 5335 1239 486 234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7294 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3033 3065 3033 Size: 32 7262 AlwNI CAG_nnn'CTG Cuts at: 2798 2881 3623 4255 5037 6609 2798 Size: 83 742 632 782 1572 3483 Fragments arranged by size: 3483 1572 782 742 632 83 ApaI G_GGCC'C Cuts at: 2672 2703 4342 2672 Size: 31 1639 5624 Fragments arranged by size: 5624 1639 31 ApaLI G'TGCA_C Cuts at: 2516 5351 6507 2516 Size: 2835 1156 3303 Fragments arranged by size: 3303 2835 1156 ApoI r'AATT_y Cuts at: 1493 4603 4829 4981 7090 1493 Size: 3110 226 152 2109 1697 Fragments arranged by size: 3110 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5848 160 Size: 872 4816 1606 Fragments arranged by size: 4816 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7294 AvaI C'yCGr_G Cuts at: 1870 1943 2671 2856 3178 4341 4619 1870 Size: 73 728 185 322 1163 278 4545 Fragments arranged by size: 4545 1163 728 322 278 185 73 BamHI G'GATC_C Cuts at: 2229 3962 4624 4991 2229 Size: 1733 662 367 4532 Fragments arranged by size: 4532 1733 662 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2037 2133 2468 2633 3221 Size: 325 217 627 250 96 335 165 588 Cuts at: 3221 3641 3800 3844 4293 4615 6070 618 Size: 420 159 44 449 322 1455 1842 Fragments arranged by size: 1842 1455 627 588 449 420 335 325 322 250 217 165 159 96 44 BanII G_rGCy'C Cuts at: 1391 2442 2672 2703 3248 3614 3842 4230 4290 Size: 1051 230 31 545 366 228 388 60 Cuts at: 4290 4342 4613 1391 Size: 52 271 4072 Fragments arranged by size: 4072 1051 545 388 366 271 230 228 60 52 31 BbeI G_GCGC'C Cuts at: 3804 3804 Size: 7294 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2173 4231 4446 928 Size: 971 274 2058 215 3776 Fragments arranged by size: 3776 2058 971 274 215 BbvCI CC'TCA_GC Cuts at: 2285 2799 2285 Size: 514 6780 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5485 5519 1903 Size: 34 3548 34 3678 Fragments arranged by size: 3678 3548 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4063 5186 6396 4063 Size: 1123 1210 4961 Fragments arranged by size: 4961 1210 1123 BclI T'GATC_A Cuts at: 1548 4461 1548 Size: 2913 4381 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3976 5905 136 Size: 107 122 71 631 605 2304 1929 1525 Fragments arranged by size: 2304 1929 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7289 7289 Size: 7294 BlpI GC'TnA_GC Cuts at: 3530 4711 3530 Size: 1181 6113 Bme1580I G_kGCm'C Cuts at: 1790 2471 2520 2636 2672 2703 3606 4342 5355 Size: 681 49 116 36 31 903 736 1013 Cuts at: 5355 6511 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1013 903 736 681 116 49 36 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7294 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2302 5025 5983 Size: 416 53 83 399 24 885 2723 958 Cuts at: 5983 442 Size: 1753 Fragments arranged by size: 2723 1753 958 885 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7294 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2458 2461 3523 2458 Size: 3 1062 6229 Fragments arranged by size: 6229 1062 3 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2388 2572 2764 2956 3281 3303 3608 4118 Size: 1149 184 192 192 325 22 305 510 Cuts at: 4118 4139 4277 5954 1239 Size: 21 138 1677 2579 Fragments arranged by size: 2579 1677 1149 510 325 305 192 192 184 138 22 21 Bpu10I CC'TnA_GC Cuts at: 2084 2285 2427 2799 3769 2084 Size: 201 142 372 970 5609 Fragments arranged by size: 5609 970 372 201 142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3714 5419 6284 6582 3714 Size: 1705 865 298 4426 Fragments arranged by size: 4426 1705 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2266 5957 882 Size: 617 767 3691 2219 Fragments arranged by size: 3691 2219 767 617 BsaAI yAC'GTr Cuts at: 493 1204 2493 5085 493 Size: 711 1289 2592 2702 Fragments arranged by size: 2702 2592 1289 711 BsaBI GATnn'nnATC Cuts at: 7288 7288 Size: 7294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3801 5101 5483 6118 Size: 53 83 186 936 2268 1300 382 635 Cuts at: 6118 275 Size: 1451 Fragments arranged by size: 2268 1451 1300 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2571 3912 4290 5727 6125 6399 6546 6957 2571 Size: 1341 378 1437 398 274 147 411 2908 Fragments arranged by size: 2908 1437 1341 411 398 378 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2246 2276 3456 3486 3530 Size: 30 165 30 843 30 1180 30 44 Cuts at: 3530 3560 1178 Size: 30 4912 Fragments arranged by size: 4912 1180 843 165 44 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2434 2450 2642 3242 3302 3305 3308 3311 Size: 1248 16 192 600 60 3 3 3 Cuts at: 3311 3604 3667 3679 4061 4136 4289 4343 1186 Size: 293 63 12 382 75 153 54 4137 Fragments arranged by size: 4137 1248 600 382 293 192 153 75 63 60 54 16 12 3 3 3 BseYI C'CCAG_C Cuts at: 2009 2224 2483 2665 2774 2944 3237 3329 3452 Size: 215 259 182 109 170 293 92 123 Cuts at: 3452 3777 3937 6497 6993 2009 Size: 325 160 2560 496 2310 Fragments arranged by size: 2560 2310 496 325 293 259 215 182 170 160 123 109 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2111 2546 2667 3020 3149 3309 3368 Size: 573 375 435 121 353 129 160 59 Cuts at: 3368 3597 1163 Size: 229 4860 Fragments arranged by size: 4860 573 435 375 353 229 160 129 121 59 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2684 2837 4744 5505 6533 1540 Size: 275 186 683 153 1907 761 1028 2301 Fragments arranged by size: 2301 1907 1028 761 683 275 186 153 BsiHKAI G_wGCw'C Cuts at: 2442 2520 2588 2788 2939 3614 4104 4230 4290 Size: 78 68 200 151 675 490 126 60 Cuts at: 4290 5355 5440 6511 2442 Size: 1065 85 1071 3225 Fragments arranged by size: 3225 1071 1065 675 490 200 151 126 85 78 68 60 BsmI GAATG_Cn' Cuts at: 4805 4898 4805 Size: 93 7201 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2442 2471 2520 2588 2636 2672 2703 Size: 399 652 29 49 68 48 36 31 Cuts at: 2703 2788 2939 3248 3606 3614 3842 4104 4230 Size: 85 151 309 358 8 228 262 126 Cuts at: 4230 4290 4342 4613 5355 5440 6511 1391 Size: 60 52 271 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 652 399 358 309 271 262 228 151 126 85 85 68 60 52 49 48 36 31 29 8 BspEI T'CCGG_A Cuts at: 6957 6957 Size: 7294 BspHI T'CATG_A Cuts at: 2292 5183 6913 2292 Size: 2891 1730 2673 Fragments arranged by size: 2891 2673 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2154 3196 3563 3868 3934 3991 4649 844 Size: 1310 1042 367 305 66 57 658 3489 Fragments arranged by size: 3489 1310 1042 658 367 305 66 57 BsrBI CCG'CTC Cuts at: 1394 1823 2237 2970 5181 1394 Size: 429 414 733 2211 3507 Fragments arranged by size: 3507 2211 733 429 414 BsrDI GCAATG_nn' Cuts at: 65 1248 5789 5963 65 Size: 1183 4541 174 1396 Fragments arranged by size: 4541 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2130 2136 2471 4083 4419 5938 6125 Size: 110 320 6 335 1612 336 1519 187 Cuts at: 6125 1700 Size: 2869 Fragments arranged by size: 2869 1612 1519 336 335 320 187 110 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7294 BssHII G'CGCG_C Cuts at: 1925 2022 3445 1925 Size: 97 1423 5774 Fragments arranged by size: 5774 1423 97 BssSI C'ACGA_G Cuts at: 4193 5354 6366 4193 Size: 1161 1012 5121 Fragments arranged by size: 5121 1161 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2493 2515 2524 2747 3043 794 Size: 1699 22 9 223 296 5045 Fragments arranged by size: 5045 1699 296 223 22 9 BstBI TT'CG_AA Cuts at: 6098 6098 Size: 7294 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7294 BstXI CCAn_nnnn'nTGG Cuts at: 2257 2407 3638 2257 Size: 150 1231 5913 Fragments arranged by size: 5913 1231 150 BstYI r'GATC_y Cuts at: 1972 2073 2229 2841 3962 4624 4991 5376 5393 Size: 101 156 612 1121 662 367 385 17 Cuts at: 5393 6845 6931 6943 7289 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1121 662 612 385 367 346 156 101 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2446 2625 2826 4044 4497 2446 Size: 179 201 1218 453 5243 Fragments arranged by size: 5243 1218 453 201 179 BtgI C'CryG_G Cuts at: 513 1065 2004 2015 2062 3113 4057 4105 513 Size: 552 939 11 47 1051 944 48 3702 Fragments arranged by size: 3702 1051 944 939 552 48 47 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2543 3504 487 Size: 683 56 1317 961 4277 Fragments arranged by size: 4277 1317 961 683 56 BtsI GCAGTG_nn' Cuts at: 2563 3802 4810 5604 5624 2563 Size: 1239 1008 794 20 4233 Fragments arranged by size: 4233 1239 1008 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7294 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7259 DraI TTT'AAA Cuts at: 4598 4598 Size: 7294 DraIII CAC_nnn'GTG Cuts at: 2524 2524 Size: 7294 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2302 6301 809 Size: 507 986 3999 1802 Fragments arranged by size: 3999 1802 986 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2335 2473 2834 Size: 54 1425 50 275 18 505 138 361 Cuts at: 2834 2883 3252 3336 3510 4741 5630 7259 8 Size: 49 369 84 174 1231 889 1629 43 Fragments arranged by size: 1629 1425 1231 889 505 369 361 275 174 138 84 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2834 4741 1537 Size: 275 1022 1907 4090 Fragments arranged by size: 4090 1907 1022 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3306 4428 5224 1353 Size: 1953 1122 796 3423 Fragments arranged by size: 3423 1953 1122 796 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5881 6255 6401 387 Size: 1064 138 4292 374 146 1280 Fragments arranged by size: 4292 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2440 3612 4228 4288 2440 Size: 1172 616 60 5446 Fragments arranged by size: 5446 1172 616 60 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 2572 2764 2956 3281 3303 3608 Size: 393 756 184 192 192 325 22 305 Cuts at: 3608 4118 4139 4277 5357 5954 6741 7009 1239 Size: 510 21 138 1080 597 787 268 1524 Fragments arranged by size: 1524 1080 787 756 597 510 393 325 305 268 192 192 184 138 22 21 EcoNI CCTnn'n_nnAGG Cuts at: 4145 4412 4145 Size: 267 7027 EcoO109I rG'GnC_Cy Cuts at: 2365 2700 3106 3773 2365 Size: 335 406 667 5886 Fragments arranged by size: 5886 667 406 335 EcoRI G'AATT_C Cuts at: 4603 4981 4603 Size: 378 6916 FspI TGC'GCA Cuts at: 2497 5800 2497 Size: 3303 3991 HaeII r_GCGC'y Cuts at: 1678 2533 3436 3530 3561 3804 4084 4267 6441 Size: 855 903 94 31 243 280 183 2174 Cuts at: 6441 1678 Size: 2531 Fragments arranged by size: 2531 2174 903 855 280 243 183 94 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2221 2246 2253 2278 3530 Size: 32 135 32 649 25 7 25 1252 Cuts at: 3530 3562 5935 5967 6009 6041 1373 Size: 32 2373 32 42 32 2626 Fragments arranged by size: 2626 2373 1252 649 135 42 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 4638 4884 669 Size: 988 281 2700 246 3079 Fragments arranged by size: 3079 2700 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7294 HpaI GTT'AAC Cuts at: 4884 4884 Size: 7294 KasI G'GCGC_C Cuts at: 3800 3800 Size: 7294 KpnI G_GTAC'C Cuts at: 3645 4619 3645 Size: 974 6320 MfeI C'AATT_G Cuts at: 4893 4893 Size: 7294 MluI A'CGCG_T Cuts at: 7170 7170 Size: 7294 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3808 4431 6408 6592 1305 Size: 2503 623 1977 184 2007 Fragments arranged by size: 2503 2007 1977 623 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2337 2885 3338 10 Size: 54 1425 343 505 548 453 3966 Fragments arranged by size: 3966 1425 548 505 453 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2405 2639 3125 4169 4532 5252 5611 Size: 648 1239 234 486 1044 363 720 359 Cuts at: 5611 5770 518 Size: 159 2042 Fragments arranged by size: 2042 1239 1044 720 648 486 363 359 234 159 MspA1I CmG'CkG Cuts at: 1606 2017 2064 3323 3599 3874 4710 5387 6535 Size: 411 47 1259 276 275 836 677 1148 Cuts at: 6535 6780 1606 Size: 245 2120 Fragments arranged by size: 2120 1259 1148 836 677 411 276 275 245 47 NaeI GCC'GGC Cuts at: 4085 4085 Size: 7294 NarI GG'CG_CC Cuts at: 3801 3801 Size: 7294 NcoI C'CATG_G Cuts at: 513 1065 2004 3113 4057 513 Size: 552 939 1109 944 3750 Fragments arranged by size: 3750 1109 944 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7294 NgoMIV G'CCGG_C Cuts at: 4083 4083 Size: 7294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7294 NotI GC'GGCC_GC Cuts at: 4741 4741 Size: 7294 NspI r_CATG'y Cuts at: 1132 1229 2516 4168 4652 6197 1132 Size: 97 1287 1652 484 1545 2229 Fragments arranged by size: 2229 1652 1545 1287 484 97 PasI CC'CwG_GG Cuts at: 1348 2603 3352 1348 Size: 1255 749 5290 Fragments arranged by size: 5290 1255 749 PciI A'CATG_T Cuts at: 6193 6193 Size: 7294 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7294 PfoI T'CCnGG_A Cuts at: 2097 2241 2761 2097 Size: 144 520 6630 Fragments arranged by size: 6630 520 144 PmeI GTTT'AAAC Cuts at: 4598 4598 Size: 7294 PmlI CAC'GTG Cuts at: 2493 2493 Size: 7294 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5363 5395 6876 6908 5363 Size: 32 1481 32 5749 Fragments arranged by size: 5749 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3106 3773 3106 Size: 667 6627 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7294 PsiI TTA'TAA Cuts at: 4864 4864 Size: 7294 PspOMI G'GGCC_C Cuts at: 2668 2699 4338 2668 Size: 31 1639 5624 Fragments arranged by size: 5624 1639 31 PstI C_TGCA'G Cuts at: 830 2330 2794 2982 3574 3797 3879 3931 4646 Size: 1500 464 188 592 223 82 52 715 Cuts at: 4646 830 Size: 3478 Fragments arranged by size: 3478 1500 715 592 464 223 188 82 52 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7294 PvuII CAG'CTG Cuts at: 3323 3599 3874 3323 Size: 276 275 6743 Fragments arranged by size: 6743 276 275 SacI G_AGCT'C Cuts at: 2442 3614 4230 4290 2442 Size: 1172 616 60 5446 Fragments arranged by size: 5446 1172 616 60 SacII CC_GC'GG Cuts at: 2018 2065 2018 Size: 47 7247 SalI G'TCGA_C Cuts at: 1936 4636 1936 Size: 2700 4594 SbfI CC_TGCA'GG Cuts at: 2794 4646 2794 Size: 1852 5442 ScaI AGT'ACT Cuts at: 5542 7254 5542 Size: 1712 5582 SexAI A'CCwGG_T Cuts at: 2991 2991 Size: 7294 SfcI C'TryA_G Cuts at: 826 1045 2326 2391 2790 2873 2978 3570 3793 Size: 219 1281 65 399 83 105 592 223 Cuts at: 3793 3875 3927 4642 5777 6458 6649 826 Size: 82 52 715 1135 681 191 1471 Fragments arranged by size: 1471 1281 1135 715 681 592 399 223 219 191 105 83 82 65 52 SfoI GGC'GCC Cuts at: 3802 3802 Size: 7294 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7294 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7294 SmaI CCC'GGG Cuts at: 1872 2673 2858 3180 4343 4621 1872 Size: 801 185 322 1163 278 4545 Fragments arranged by size: 4545 1163 801 322 278 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 3729 5398 6299 6561 820 Size: 197 926 1786 1669 901 262 1553 Fragments arranged by size: 1786 1669 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5085 493 Size: 4592 2702 SpeI A'CTAG_T Cuts at: 152 3700 152 Size: 3548 3746 SphI G_CATG'C Cuts at: 1132 4652 1132 Size: 3520 3774 SspI AAT'ATT Cuts at: 5 52 5218 5 Size: 47 5166 2081 Fragments arranged by size: 5166 2081 47 StuI AGG'CCT Cuts at: 1517 2695 2927 1517 Size: 1178 232 5884 Fragments arranged by size: 5884 1178 232 StyI C'CwwG_G Cuts at: 513 1065 2004 2169 2751 2814 3113 3957 4057 Size: 552 939 165 582 63 299 844 100 Cuts at: 4057 4733 513 Size: 676 3074 Fragments arranged by size: 3074 939 844 676 582 552 299 165 100 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2371 2413 3343 3388 3466 5321 5338 Size: 1255 791 42 930 45 78 1855 17 Cuts at: 5338 5491 5676 6121 325 Size: 153 185 445 1498 Fragments arranged by size: 1855 1498 1255 930 791 445 185 153 78 45 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4035 5540 7252 Size: 274 80 33 51 1443 2058 1505 1712 Cuts at: 7252 96 Size: 138 Fragments arranged by size: 2058 1712 1505 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3350 3382 3456 3488 7254 7286 3350 Size: 32 74 32 3766 32 3358 Fragments arranged by size: 3766 3358 74 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2177 2258 2680 1316 Size: 861 81 422 5930 Fragments arranged by size: 5930 861 422 81 XbaI T'CTAG_A Cuts at: 4630 4630 Size: 7294 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3349 3679 4317 3349 Size: 330 638 6326 Fragments arranged by size: 6326 638 330 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7294 XmaI C'CCGG_G Cuts at: 1870 2671 2856 3178 4341 4619 1870 Size: 801 185 322 1163 278 4545 Fragments arranged by size: 4545 1163 801 322 278 185 XmnI GAAnn'nnTTC Cuts at: 4475 5423 4475 Size: 948 6346 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5102 276 Size: 53 83 186 936 3568 2468 Fragments arranged by size: 3568 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI