Restriction map for
pFN21AA1668
(Linear) (Six-base) MAPSORT of: KIAA1668.seq Check: 4707 from: 1 to: 2589
KIAA1668.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1191 1558 1929 1986 2589
Size: 1191 367 371 57 603
Fragments arranged by size:
1191 603 371 367 57
Acc65I G'GTAC_C
Cuts at: 0 1636 2589
Size: 1636 953
AfeI AGC'GCT
Cuts at: 0 526 1523 2589
Size: 526 997 1066
Fragments arranged by size:
1066 997 526
AleI CACnn'nnGTG
Cuts at: 0 400 634 1120 2589
Size: 400 234 486 1469
Fragments arranged by size:
1469 486 400 234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1028 1060 2589
Size: 1028 32 1529
Fragments arranged by size:
1529 1028 32
AlwNI CAG_nnn'CTG
Cuts at: 0 793 876 1618 2250 2589
Size: 793 83 742 632 339
Fragments arranged by size:
793 742 632 339 83
ApaI G_GGCC'C
Cuts at: 0 667 698 2337 2589
Size: 667 31 1639 252
Fragments arranged by size:
1639 667 252 31
ApaLI G'TGCA_C
Cuts at: 0 511 2589
Size: 511 2078
AvaI C'yCGr_G
Cuts at: 0 666 851 1173 2336 2589
Size: 666 185 322 1163 253
Fragments arranged by size:
1163 666 322 253 185
BamHI G'GATC_C
Cuts at: 0 224 1957 2589
Size: 224 1733 632
Fragments arranged by size:
1733 632 224
BanI G'GyrC_C
Cuts at: 0 32 128 463 628 1216 1636 1795 1839
Size: 32 96 335 165 588 420 159 44
Cuts at: 1839 2288 2589
Size: 449 301
Fragments arranged by size:
588 449 420 335 301 165 159 96
44 32
BanII G_rGCy'C
Cuts at: 0 437 667 698 1243 1609 1837 2225 2285
Size: 437 230 31 545 366 228 388 60
Cuts at: 2285 2337 2589
Size: 52 252
Fragments arranged by size:
545 437 388 366 252 230 228 60
52 31
BbeI G_GCGC'C
Cuts at: 0 1799 2589
Size: 1799 790
BbsI GAAGACnn'nnnn_
Cuts at: 0 168 2226 2441 2589
Size: 168 2058 215 148
Fragments arranged by size:
2058 215 168 148
BbvCI CC'TCA_GC
Cuts at: 0 280 794 2589
Size: 280 514 1795
Fragments arranged by size:
1795 514 280
BciVI GTATCCnnnnn_n'
Cuts at: 0 2058 2589
Size: 2058 531
BclI T'GATC_A
Cuts at: 0 2456 2589
Size: 2456 133
BglI GCCn_nnn'nGGC
Cuts at: 0 1971 2589
Size: 1971 618
BlpI GC'TnA_GC
Cuts at: 0 1525 2589
Size: 1525 1064
Bme1580I G_kGCm'C
Cuts at: 0 466 515 631 667 698 1601 2337 2589
Size: 466 49 116 36 31 903 736 252
Fragments arranged by size:
903 736 466 252 116 49 36 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 297 2589
Size: 297 2292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 453 456 1518 2589
Size: 453 3 1062 1071
Fragments arranged by size:
1071 1062 453 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 567 759 951 1276 1298 1603 2113
Size: 383 184 192 192 325 22 305 510
Cuts at: 2113 2134 2272 2589
Size: 21 138 317
Fragments arranged by size:
510 383 325 317 305 192 192 184
138 22 21
Bpu10I CC'TnA_GC
Cuts at: 0 79 280 422 794 1764 2589
Size: 79 201 142 372 970 825
Fragments arranged by size:
970 825 372 201 142 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1709 2589
Size: 1709 880
BsaI GGTCTCn'nnnn_
Cuts at: 0 261 2589
Size: 261 2328
BsaAI yAC'GTr
Cuts at: 0 488 2589
Size: 488 2101
BsaHI Gr'CG_yC
Cuts at: 0 1796 2589
Size: 1796 793
BsaWI w'CCGG_w
Cuts at: 0 566 1907 2285 2589
Size: 566 1341 378 304
Fragments arranged by size:
1341 566 378 304
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 241 271 1451 1481 1525 1555 2589
Size: 241 30 1180 30 44 30 1034
Fragments arranged by size:
1180 1034 241 44 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 429 445 637 1237 1297 1300 1303 1306
Size: 429 16 192 600 60 3 3 3
Cuts at: 1306 1599 1662 1674 2056 2131 2284 2338 2589
Size: 293 63 12 382 75 153 54 251
Fragments arranged by size:
600 429 382 293 251 192 153 75
63 60 54 16 12 3 3 3
BseYI C'CCAG_C
Cuts at: 0 4 219 478 660 769 939 1232 1324
Size: 4 215 259 182 109 170 293 92
Cuts at: 1324 1447 1772 1932 2589
Size: 123 325 160 657
Fragments arranged by size:
657 325 293 259 215 182 170 160
123 109 92 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 541 662 1015 1144 1304 1363 1592
Size: 106 435 121 353 129 160 59 229
Cuts at: 1592 2589
Size: 997
Fragments arranged by size:
997 435 353 229 160 129 121 106
59
BsiEI CG_ry'CG
Cuts at: 0 679 832 2589
Size: 679 153 1757
Fragments arranged by size:
1757 679 153
BsiHKAI G_wGCw'C
Cuts at: 0 437 515 583 783 934 1609 2099 2225
Size: 437 78 68 200 151 675 490 126
Cuts at: 2225 2285 2589
Size: 60 304
Fragments arranged by size:
675 490 437 304 200 151 126 78
68 60
Bsp1286I G_dGCh'C
Cuts at: 0 437 466 515 583 631 667 698 783
Size: 437 29 49 68 48 36 31 85
Cuts at: 783 934 1243 1601 1609 1837 2099 2225 2285
Size: 151 309 358 8 228 262 126 60
Cuts at: 2285 2337 2589
Size: 52 252
Fragments arranged by size:
437 358 309 262 252 228 151 126
85 68 60 52 49 48 36 31
29 8
BspHI T'CATG_A
Cuts at: 0 287 2589
Size: 287 2302
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 1191 1558 1863 1929 1986 2589
Size: 149 1042 367 305 66 57 603
Fragments arranged by size:
1042 603 367 305 149 66 57
BsrBI CCG'CTC
Cuts at: 0 232 965 2589
Size: 232 733 1624
Fragments arranged by size:
1624 733 232
BsrFI r'CCGG_y
Cuts at: 0 125 131 466 2078 2414 2589
Size: 125 6 335 1612 336 175
Fragments arranged by size:
1612 336 335 175 125 6
BssHII G'CGCG_C
Cuts at: 0 17 1440 2589
Size: 17 1423 1149
Fragments arranged by size:
1423 1149 17
BssSI C'ACGA_G
Cuts at: 0 2188 2589
Size: 2188 401
BstAPI GCAn_nnn'nTGC
Cuts at: 0 488 510 519 742 1038 2589
Size: 488 22 9 223 296 1551
Fragments arranged by size:
1551 488 296 223 22 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 252 402 1633 2589
Size: 252 150 1231 956
Fragments arranged by size:
1231 956 252 150
BstYI r'GATC_y
Cuts at: 0 68 224 836 1957 2589
Size: 68 156 612 1121 632
Fragments arranged by size:
1121 632 612 156 68
Bsu36I CC'TnA_GG
Cuts at: 0 441 620 821 2039 2492 2589
Size: 441 179 201 1218 453 97
Fragments arranged by size:
1218 453 441 201 179 97
BtgI C'CryG_G
Cuts at: 0 10 57 1108 2052 2100 2589
Size: 10 47 1051 944 48 489
Fragments arranged by size:
1051 944 489 48 47 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 538 1499 2589
Size: 538 961 1090
Fragments arranged by size:
1090 961 538
BtsI GCAGTG_nn'
Cuts at: 0 558 1797 2589
Size: 558 1239 792
Fragments arranged by size:
1239 792 558
DraIII CAC_nnn'GTG
Cuts at: 0 519 2589
Size: 519 2070
DrdI GACnn_nn'nnGTC
Cuts at: 0 297 2589
Size: 297 2292
EaeI y'GGCC_r
Cuts at: 0 330 468 829 878 1247 1331 1505 2589
Size: 330 138 361 49 369 84 174 1084
Fragments arranged by size:
1084 369 361 330 174 138 84 49
EagI C'GGCC_G
Cuts at: 0 829 2589
Size: 829 1760
EarI CTCTTCn'nnn_
Cuts at: 0 1301 2423 2589
Size: 1301 1122 166
Fragments arranged by size:
1301 1122 166
EcoICRI GAG'CTC
Cuts at: 0 435 1607 2223 2283 2589
Size: 435 1172 616 60 306
Fragments arranged by size:
1172 616 435 306 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 567 759 951 1276 1298 1603 2113
Size: 383 184 192 192 325 22 305 510
Cuts at: 2113 2134 2272 2589
Size: 21 138 317
Fragments arranged by size:
510 383 325 317 305 192 192 184
138 22 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2140 2407 2589
Size: 2140 267 182
Fragments arranged by size:
2140 267 182
EcoO109I rG'GnC_Cy
Cuts at: 0 360 695 1101 1768 2589
Size: 360 335 406 667 821
Fragments arranged by size:
821 667 406 360 335
FspI TGC'GCA
Cuts at: 0 492 2589
Size: 492 2097
HaeII r_GCGC'y
Cuts at: 0 528 1431 1525 1556 1799 2079 2262 2589
Size: 528 903 94 31 243 280 183 327
Fragments arranged by size:
903 528 327 280 243 183 94 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 216 241 248 273 1525 1557 2589
Size: 216 25 7 25 1252 32 1032
Fragments arranged by size:
1252 1032 216 32 25 25 7
KasI G'GCGC_C
Cuts at: 0 1795 2589
Size: 1795 794
KpnI G_GTAC'C
Cuts at: 0 1640 2589
Size: 1640 949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1803 2426 2589
Size: 1803 623 163
Fragments arranged by size:
1803 623 163
MscI TGG'CCA
Cuts at: 0 332 880 1333 2589
Size: 332 548 453 1256
Fragments arranged by size:
1256 548 453 332
MslI CAynn'nnrTG
Cuts at: 0 400 634 1120 2164 2527 2589
Size: 400 234 486 1044 363 62
Fragments arranged by size:
1044 486 400 363 234 62
MspA1I CmG'CkG
Cuts at: 0 12 59 1318 1594 1869 2589
Size: 12 47 1259 276 275 720
Fragments arranged by size:
1259 720 276 275 47 12
NaeI GCC'GGC
Cuts at: 0 2080 2589
Size: 2080 509
NarI GG'CG_CC
Cuts at: 0 1796 2589
Size: 1796 793
NcoI C'CATG_G
Cuts at: 0 1108 2052 2589
Size: 1108 944 537
Fragments arranged by size:
1108 944 537
NgoMIV G'CCGG_C
Cuts at: 0 2078 2589
Size: 2078 511
NspI r_CATG'y
Cuts at: 0 511 2163 2589
Size: 511 1652 426
Fragments arranged by size:
1652 511 426
PasI CC'CwG_GG
Cuts at: 0 598 1347 2589
Size: 598 749 1242
Fragments arranged by size:
1242 749 598
PfoI T'CCnGG_A
Cuts at: 0 92 236 756 2589
Size: 92 144 520 1833
Fragments arranged by size:
1833 520 144 92
PmlI CAC'GTG
Cuts at: 0 488 2589
Size: 488 2101
PpuMI rG'GwC_Cy
Cuts at: 0 1101 1768 2589
Size: 1101 667 821
Fragments arranged by size:
1101 821 667
PspOMI G'GGCC_C
Cuts at: 0 663 694 2333 2589
Size: 663 31 1639 256
Fragments arranged by size:
1639 663 256 31
PstI C_TGCA'G
Cuts at: 0 325 789 977 1569 1792 1874 1926 2589
Size: 325 464 188 592 223 82 52 663
Fragments arranged by size:
663 592 464 325 223 188 82 52
PvuII CAG'CTG
Cuts at: 0 1318 1594 1869 2589
Size: 1318 276 275 720
Fragments arranged by size:
1318 720 276 275
SacI G_AGCT'C
Cuts at: 0 437 1609 2225 2285 2589
Size: 437 1172 616 60 304
Fragments arranged by size:
1172 616 437 304 60
SacII CC_GC'GG
Cuts at: 0 13 60 2589
Size: 13 47 2529
Fragments arranged by size:
2529 47 13
SbfI CC_TGCA'GG
Cuts at: 0 789 2589
Size: 789 1800
SexAI A'CCwGG_T
Cuts at: 0 986 2589
Size: 986 1603
SfcI C'TryA_G
Cuts at: 0 321 386 785 868 973 1565 1788 1870
Size: 321 65 399 83 105 592 223 82
Cuts at: 1870 1922 2589
Size: 52 667
Fragments arranged by size:
667 592 399 321 223 105 83 82
65 52
SfoI GGC'GCC
Cuts at: 0 1797 2589
Size: 1797 792
SmaI CCC'GGG
Cuts at: 0 668 853 1175 2338 2589
Size: 668 185 322 1163 251
Fragments arranged by size:
1163 668 322 251 185
SmlI C'TyrA_G
Cuts at: 0 1724 2589
Size: 1724 865
SpeI A'CTAG_T
Cuts at: 0 1695 2589
Size: 1695 894
StuI AGG'CCT
Cuts at: 0 690 922 2589
Size: 690 232 1667
Fragments arranged by size:
1667 690 232
StyI C'CwwG_G
Cuts at: 0 164 746 809 1108 1952 2052 2589
Size: 164 582 63 299 844 100 537
Fragments arranged by size:
844 582 537 299 164 100 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 366 408 1338 1383 1461 2589
Size: 366 42 930 45 78 1128
Fragments arranged by size:
1128 930 366 78 45 42
TatI w'GTAC_w
Cuts at: 0 2030 2589
Size: 2030 559
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1345 1377 1451 1483 2589
Size: 1345 32 74 32 1106
Fragments arranged by size:
1345 1106 74 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 172 253 675 2589
Size: 172 81 422 1914
Fragments arranged by size:
1914 422 172 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1344 1674 2312 2589
Size: 1344 330 638 277
Fragments arranged by size:
1344 638 330 277
XmaI C'CCGG_G
Cuts at: 0 666 851 1173 2336 2589
Size: 666 185 322 1163 253
Fragments arranged by size:
1163 666 322 253 185
XmnI GAAnn'nnTTC
Cuts at: 0 2470 2589
Size: 2470 119
Enzymes that do cut:
AarI Acc65I AfeI AleI AloI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SacII SbfI SexAI SfcI SfoI SmaI SmlI SpeI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AhdI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BcgI
BfrBI BglII BmgBI BmtI BsaBI BsiWI BsmI BsmBI
BspEI BsrDI BsrGI BstBI BstEII BstZ17I ClaI CspCI
DraI EciI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI
SfiI SgfI SgrAI SnaBI SphI SrfI SspI SwaI
XbaI XhoI ZraI