Restriction map for
pFN21AA1619
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BalI
BcgI BclI BfrI BglII BlnI
BsaBI BsiEI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FbaI FseI
HgaI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI NcoI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 2061 CGCG CG^CG
AccIII 563 TCCGGA T^CCGGA
AcsI 559 GAATTC [GA]^AATT[CT]
AcyI 2135 GGCGCC G[GA]^CG[CT]C
AflIII 1240 ACACGT A^C[GA][CT]GT
AlwNI 41 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2440 GGGCCC GGGCC^C
ApoI 559 GAATTC [GA]^AATT[CT]
BamHI 1361 GGATCC G^GATCC
BbeI 2135 GGCGCC GGCGC^C
BbrPI 1241 CACGTG CAC^GTG
BciVI 1698 GGATAC GTATCC(6/5)
BsaAI 1241 CACGTG [CT]AC^GT[GA]
BsaHI 2135 GGCGCC G[GA]^CG[CT]C
BsaWI 563 TCCGGA [AT]^CCGG[AT]
BseAI 563 TCCGGA T^CCGGA
BsmBI 871 CGTCTC CGTCTC(1/5)
BsmI 569 GCATTC GAATGC(1/-1)
BspEI 563 TCCGGA T^CCGGA
BspMI 1331 ACCTGC ACCTGC(4/8)
BsrBI 1728 CCGCTC CCGCTC(-3/-3)
BstUI 2061 CGCG CG^CG
DsaI 504 CCGTGG C^C[GA][CT]GG
EaeI 1966 CGGCCA [CT]^GGCC[GA]
EcoNI 191 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 559 GAATTC G^AATTC
EcoT14I 1152 CCAAGG C^C[AT][AT]GG
Hin1I 2135 GGCGCC G[GA]^CG[CT]C
KasI 2135 GGCGCC G^GCGCC
MaeII 1242 ACGT A^CGT
MroI 563 TCCGGA T^CCGGA
MseI 347 TTAA T^TAA
MvnI 2061 CGCG CG^CG
NaeI 2372 GCCGGC GCC^GGC
NarI 2135 GGCGCC GG^CGCC
NgoMIV 2372 GCCGGC G^CCGGC
NheI 2049 GCTAGC G^CTAGC
NspI 394 GCATGT [GA]CATG^[CT]
PmaCI 1241 CACGTG CAC^GTG
PmlI 1241 CACGTG CAC^GTG
PshAI 1296 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 121 GAAGAGC GCTCTTC(1/4)
SfoI 2135 GGCGCC GGC^GCC
SmaI 1787 CCCGGG CCC^GGG
StyI 1152 CCAAGG C^C[AT][AT]GG
TfiI 1138 GATTC G^A[AT]TC
Tru9I 347 TTAA T^TAA
XbaI 79 TCTAGA T^CTAGA
XmaCI 1787 CCCGGG C^CCGGG
XmaI 1787 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AviII 1145 TGCGCA TGC^GCA
1600 TGCGCA
BbsI 984 GAAGAC GAAGAC(2/6)
1722 GAAGAC
BlpI 969 GCTGAGC GC^T[ACGT]AGC
2338 GCTGAGC
Bpu1102I 969 GCTGAGC GC^T[ACGT]AGC
2338 GCTGAGC
BpuAI 984 GAAGAC GAAGAC(2/6)
1722 GAAGAC
BsgI 425 GTGCAG GTGCAG(16/14)
689 GTGCAG
BsrDI 1142 CATTGC GCAATG(2/0)
2055 CATTGC
BsrFI 2334 ACCGGC [GA]^CCGG[CT]
2372 GCCGGC
CelII 969 GCTGAGC GC^T[ACGT]AGC
2338 GCTGAGC
Cfr10I 2334 ACCGGC [GA]^CCGG[CT]
2372 GCCGGC
EarI 121 GAAGAG CTCTTC(1/4)
1405 GAAGAG
EcoT22I 392 ATGCAT ATGCA^T
628 ATGCAT
FspI 1145 TGCGCA TGC^GCA
1600 TGCGCA
HaeII 1079 GGCGCT [GA]GCGC^[CT]
2135 GGCGCC
Ksp632I 121 GAAGAG CTCTTC(1/4)
1405 GAAGAG
MslI 385 CACCTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
949 CACTTCTATG
NsiI 392 ATGCAT ATGCA^T
628 ATGCAT
PflMI 499 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1431 CCAGTCTGTGG
SacI 124 GAGCTC GAGCT^C
2520 GAGCTC
SmlI 936 CTCAAG C^T[CT][GA]AG
1261 CTCAAG
TaqI 467 TCGA T^CGA
1677 TCGA
TthHB8I 467 TCGA T^CGA
1677 TCGA
Van91I 499 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1431 CCAGTCTGTGG
XcmI 2277 CCAGATCATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2348 CCAATGGCTTGGTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TseI Tsp45I
Tsp509I TspRI XhoII