(Circular) (Six-base) MAPSORT of: pFN21AA1619.seq Check: 9920 from: 1 to: 7234 pFN21AA1619 7234 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5044 278 Size: 53 83 186 936 3508 2468 Fragments arranged by size: 3508 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4577 1937 Size: 2640 4594 Acc65I G'GTAC_C Cuts at: 4555 4555 Size: 7234 AclI AA'CG_TT Cuts at: 1563 5361 5734 1563 Size: 3798 373 3063 Fragments arranged by size: 3798 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3746 5297 6681 6949 1632 Size: 2114 1551 1384 268 1917 Fragments arranged by size: 2114 1917 1551 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7037 AflIII A'CryG_T Cuts at: 1203 3245 6133 7110 1203 Size: 2042 2888 977 1327 Fragments arranged by size: 2888 2042 1327 977 AgeI A'CCGG_T Cuts at: 6065 6065 Size: 7234 AhdI GACnn_n'nnGTC Cuts at: 5963 5963 Size: 7234 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4465 1184 Size: 3281 3953 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2645 2677 2645 Size: 32 7202 AlwNI CAG_nnn'CTG Cuts at: 2051 4977 6549 2051 Size: 2926 1572 2736 Fragments arranged by size: 2926 2736 1572 ApaI G_GGCC'C Cuts at: 4449 4449 Size: 7234 ApaLI G'TGCA_C Cuts at: 5291 6447 5291 Size: 1156 6078 ApoI r'AATT_y Cuts at: 1493 2564 4543 4769 4921 7030 1493 Size: 1071 1979 226 152 2109 1697 Fragments arranged by size: 2109 1979 1697 1071 226 152 AseI AT'TA_AT Cuts at: 160 1032 5788 160 Size: 872 4756 1606 Fragments arranged by size: 4756 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7234 AvaI C'yCGr_G Cuts at: 1870 1943 2220 3564 3792 4559 1870 Size: 73 277 1344 228 767 4545 Fragments arranged by size: 4545 1344 767 277 228 73 BamHI G'GATC_C Cuts at: 3366 4564 4931 3366 Size: 1198 367 5669 Fragments arranged by size: 5669 1198 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2225 3109 3593 4027 4140 Size: 325 217 627 438 884 484 434 113 Cuts at: 4140 4555 6010 618 Size: 415 1455 1842 Fragments arranged by size: 1842 1455 884 627 484 438 434 415 325 217 113 BanII G_rGCy'C Cuts at: 1391 2133 2221 2269 2756 3120 3392 3493 4306 Size: 742 88 48 487 364 272 101 813 Cuts at: 4306 4449 4529 4553 1391 Size: 143 80 24 4072 Fragments arranged by size: 4072 813 742 487 364 272 143 101 88 80 48 24 BbeI G_GCGC'C Cuts at: 4144 4144 Size: 7234 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2996 3734 928 Size: 971 1097 738 4428 Fragments arranged by size: 4428 1097 971 738 BbvCI CC'TCA_GC Cuts at: 2033 2197 2445 3004 2033 Size: 164 248 559 6263 Fragments arranged by size: 6263 559 248 164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5425 5459 1903 Size: 34 3488 34 3678 Fragments arranged by size: 3678 3488 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3697 5126 6336 3697 Size: 1429 1210 4595 Fragments arranged by size: 4595 1429 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7234 BfrBI ATG'CAT Cuts at: 2399 2635 2399 Size: 236 6998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2801 4175 4269 Size: 107 122 71 631 605 1129 1374 94 Cuts at: 4269 4378 5845 136 Size: 109 1467 1525 Fragments arranged by size: 1525 1467 1374 1129 631 605 122 109 107 94 71 BglII A'GATC_T Cuts at: 7229 7229 Size: 7234 BlpI GC'TnA_GC Cuts at: 2975 4344 4651 2975 Size: 1369 307 5558 Fragments arranged by size: 5558 1369 307 Bme1580I G_kGCm'C Cuts at: 1790 2230 3243 3353 4021 4449 5295 6451 1790 Size: 440 1013 110 668 428 846 1156 2573 Fragments arranged by size: 2573 1156 1013 846 668 440 428 110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7234 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2293 3430 4169 Size: 416 53 83 399 24 876 1137 739 Cuts at: 4169 4965 5923 442 Size: 796 958 1753 Fragments arranged by size: 1753 1137 958 876 796 739 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4058 1056 Size: 3002 4232 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2212 2770 2212 Size: 558 6676 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 3017 3505 4245 4265 4286 5894 1239 Size: 968 810 488 740 20 21 1608 2579 Fragments arranged by size: 2579 1608 968 810 740 488 21 20 Bpu10I CC'TnA_GC Cuts at: 2033 2197 2445 3004 3114 3827 3891 2033 Size: 164 248 559 110 713 64 5376 Fragments arranged by size: 5376 713 559 248 164 110 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2926 3251 5359 6224 6522 2926 Size: 325 2108 865 298 3638 Fragments arranged by size: 3638 2108 865 325 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2071 2814 3561 5897 882 Size: 617 572 743 747 2336 2219 Fragments arranged by size: 2336 2219 747 743 617 572 BsaAI yAC'GTr Cuts at: 493 1204 3248 5025 493 Size: 711 2044 1777 2702 Fragments arranged by size: 2702 2044 1777 711 BsaBI GATnn'nnATC Cuts at: 7228 7228 Size: 7234 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4141 5041 5423 6058 Size: 53 83 186 936 2608 900 382 635 Cuts at: 6058 275 Size: 1451 Fragments arranged by size: 2608 1451 936 900 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2568 5667 6065 6339 6486 6897 2568 Size: 3099 398 274 147 411 2905 Fragments arranged by size: 3099 2905 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2591 2621 2645 2675 3422 Size: 30 165 30 1188 30 24 30 747 Cuts at: 3422 3452 1178 Size: 30 4960 Fragments arranged by size: 4960 1188 747 165 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2018 2021 2204 2489 2636 2762 4087 4094 Size: 832 3 183 285 147 126 1325 7 Cuts at: 4094 1186 Size: 4326 Fragments arranged by size: 4326 1325 832 285 183 147 126 7 3 BseYI C'CCAG_C Cuts at: 2503 3087 3582 3988 4256 4306 4373 6437 6933 Size: 584 495 406 268 50 67 2064 496 Cuts at: 6933 2503 Size: 2804 Fragments arranged by size: 2804 2064 584 496 495 406 268 67 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2451 2715 1163 Size: 573 715 264 5682 Fragments arranged by size: 5682 715 573 264 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4684 5445 6473 1540 Size: 275 186 2683 761 1028 2301 Fragments arranged by size: 2683 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 2981 3744 3897 4529 5295 5380 6451 2133 Size: 848 763 153 632 766 85 1071 2916 Fragments arranged by size: 2916 1071 848 766 763 632 153 85 BsmI GAATG_Cn' Cuts at: 2574 4745 4838 2574 Size: 2171 93 4970 Fragments arranged by size: 4970 2171 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2882 2882 Size: 7234 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2221 2230 2269 2756 2981 3120 Size: 399 343 88 9 39 487 225 139 Cuts at: 3120 3243 3353 3392 3493 3744 3897 4021 4306 Size: 123 110 39 101 251 153 124 285 Cuts at: 4306 4449 4529 4553 5295 5380 6451 1391 Size: 143 80 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 399 343 285 251 225 153 143 139 124 123 110 101 88 85 80 39 39 24 9 BspEI T'CCGG_A Cuts at: 2568 6897 2568 Size: 4329 2905 BspHI T'CATG_A Cuts at: 5123 6853 5123 Size: 1730 5504 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3345 4589 844 Size: 2501 1244 3489 Fragments arranged by size: 3489 2501 1244 BsrBI CCG'CTC Cuts at: 1394 1823 3735 5121 1394 Size: 429 1912 1386 3507 Fragments arranged by size: 3507 1912 1386 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3146 4059 5729 5903 65 Size: 1183 1898 913 1670 174 1396 Fragments arranged by size: 1898 1670 1396 1183 913 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4339 4377 5878 6065 1700 Size: 110 2529 38 1501 187 2869 Fragments arranged by size: 2869 2529 1501 187 110 38 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7234 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7234 BssSI C'ACGA_G Cuts at: 2672 2805 3130 4505 5294 6306 2672 Size: 133 325 1375 789 1012 3600 Fragments arranged by size: 3600 1375 1012 789 325 133 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7234 BstBI TT'CG_AA Cuts at: 6038 6038 Size: 7234 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7234 BstXI CCAn_nnnn'nTGG Cuts at: 2637 2698 4223 4360 2637 Size: 61 1525 137 5511 Fragments arranged by size: 5511 1525 137 61 BstYI r'GATC_y Cuts at: 1972 2277 2838 3033 3366 4184 4564 4931 5316 Size: 305 561 195 333 818 380 367 385 Cuts at: 5316 5333 6785 6871 6883 7229 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 818 561 385 380 367 346 333 305 195 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2509 513 Size: 552 1444 5238 Fragments arranged by size: 5238 1444 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3846 487 Size: 683 56 2620 3875 Fragments arranged by size: 3875 2620 683 56 BtsI GCAGTG_nn' Cuts at: 2069 2177 2703 2906 4367 4750 5544 5564 2069 Size: 108 526 203 1461 383 794 20 3739 Fragments arranged by size: 3739 1461 794 526 383 203 108 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7234 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7199 DraI TTT'AAA Cuts at: 4538 4538 Size: 7234 DraIII CAC_nnn'GTG Cuts at: 2791 2912 2985 2791 Size: 121 73 7040 Fragments arranged by size: 7040 121 73 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6241 809 Size: 507 4925 1802 Fragments arranged by size: 4925 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3971 4681 5570 Size: 54 1425 50 275 18 2141 710 889 Cuts at: 5570 7199 8 Size: 1629 43 Fragments arranged by size: 2141 1629 1425 889 710 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4681 1537 Size: 275 2869 4090 Fragments arranged by size: 4090 2869 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 3405 5164 1353 Size: 768 1284 1759 3423 Fragments arranged by size: 3423 1759 1284 768 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2576 5821 6195 6341 387 Size: 1064 138 987 3245 374 146 1280 Fragments arranged by size: 3245 1280 1064 987 374 146 138 EcoICRI GAG'CTC Cuts at: 2131 4527 2131 Size: 2396 4838 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 3017 3505 3746 4245 4265 4286 Size: 393 575 810 488 241 499 20 21 Cuts at: 4286 5297 5894 6681 6949 1239 Size: 1011 597 787 268 1524 Fragments arranged by size: 1524 1011 810 787 597 575 499 488 393 268 241 21 20 EcoNI CCTnn'n_nnAGG Cuts at: 2200 2200 Size: 7234 EcoO109I rG'GnC_Cy Cuts at: 2063 2139 3053 3586 3633 4261 4445 4446 2063 Size: 76 914 533 47 628 184 1 4851 Fragments arranged by size: 4851 914 628 533 184 76 47 1 EcoRI G'AATT_C Cuts at: 2564 4543 4921 2564 Size: 1979 378 4877 Fragments arranged by size: 4877 1979 378 FspI TGC'GCA Cuts at: 3152 3607 5740 3152 Size: 455 2133 4646 Fragments arranged by size: 4646 2133 455 HaeII r_GCGC'y Cuts at: 1678 3088 4144 6381 1678 Size: 1410 1056 2237 2531 Fragments arranged by size: 2531 2237 1410 1056 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3170 3202 3422 3454 3664 Size: 32 135 32 1598 32 220 32 210 Cuts at: 3664 3696 3764 3796 4511 4543 5875 5907 5949 Size: 32 68 32 715 32 1332 32 42 Cuts at: 5949 5981 1373 Size: 32 2626 Fragments arranged by size: 2626 1598 1332 715 220 210 135 68 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4578 4824 669 Size: 988 281 2640 246 3079 Fragments arranged by size: 3079 2640 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7234 HpaI GTT'AAC Cuts at: 4824 4824 Size: 7234 KasI G'GCGC_C Cuts at: 4140 4140 Size: 7234 KpnI G_GTAC'C Cuts at: 4559 4559 Size: 7234 MfeI C'AATT_G Cuts at: 4833 4833 Size: 7234 MluI A'CGCG_T Cuts at: 7110 7110 Size: 7234 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3807 6348 6532 1305 Size: 2502 2541 184 2007 Fragments arranged by size: 2541 2502 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5412 Fragments arranged by size: 5412 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2394 2958 5192 5551 5710 518 Size: 648 1228 564 2234 359 159 2042 Fragments arranged by size: 2234 2042 1228 648 564 359 159 MspA1I CmG'CkG Cuts at: 1606 2374 3356 3525 4024 4275 4650 5327 6475 Size: 768 982 169 499 251 375 677 1148 Cuts at: 6475 6720 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 982 768 677 499 375 251 245 169 NaeI GCC'GGC Cuts at: 4379 4379 Size: 7234 NarI GG'CG_CC Cuts at: 4141 4141 Size: 7234 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6682 NdeI CA'TA_TG Cuts at: 387 387 Size: 7234 NgoMIV G'CCGG_C Cuts at: 4377 4377 Size: 7234 NheI G'CTAG_C Cuts at: 1052 4054 1052 Size: 3002 4232 NotI GC'GGCC_GC Cuts at: 4681 4681 Size: 7234 NsiI A_TGCA'T Cuts at: 2401 2637 2401 Size: 236 6998 NspI r_CATG'y Cuts at: 1132 1229 2403 4592 6137 1132 Size: 97 1174 2189 1545 2229 Fragments arranged by size: 2229 2189 1545 1174 97 PasI CC'CwG_GG Cuts at: 1348 3637 4292 1348 Size: 2289 655 4290 Fragments arranged by size: 4290 2289 655 PciI A'CATG_T Cuts at: 6133 6133 Size: 7234 PflMI CCAn_nnn'nTGG Cuts at: 1108 2510 3442 1108 Size: 1402 932 4900 Fragments arranged by size: 4900 1402 932 PfoI T'CCnGG_A Cuts at: 3195 4222 4479 3195 Size: 1027 257 5950 Fragments arranged by size: 5950 1027 257 PmeI GTTT'AAAC Cuts at: 4538 4538 Size: 7234 PmlI CAC'GTG Cuts at: 3248 3248 Size: 7234 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2645 2677 5303 5335 6816 6848 2645 Size: 32 2626 32 1481 32 3031 Fragments arranged by size: 3031 2626 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2063 2139 3053 3586 3633 2063 Size: 76 914 533 47 5664 Fragments arranged by size: 5664 914 533 76 47 PshAI GACnn'nnGTC Cuts at: 1613 3305 1613 Size: 1692 5542 PsiI TTA'TAA Cuts at: 4804 4804 Size: 7234 PspOMI G'GGCC_C Cuts at: 4445 4445 Size: 7234 PstI C_TGCA'G Cuts at: 830 2056 2373 2847 2924 4203 4586 830 Size: 1226 317 474 77 1279 383 3478 Fragments arranged by size: 3478 1279 1226 474 383 317 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7234 PvuII CAG'CTG Cuts at: 3356 3525 4024 4275 3356 Size: 169 499 251 6315 Fragments arranged by size: 6315 499 251 169 SacI G_AGCT'C Cuts at: 2133 4529 2133 Size: 2396 4838 SalI G'TCGA_C Cuts at: 1936 4576 1936 Size: 2640 4594 SanDI GG'GwC_CC Cuts at: 2139 3586 2139 Size: 1447 5787 SapI GCTCTTCn'nnn_ Cuts at: 2121 2121 Size: 7234 SbfI CC_TGCA'GG Cuts at: 4586 4586 Size: 7234 ScaI AGT'ACT Cuts at: 5482 7194 5482 Size: 1712 5522 SfcI C'TryA_G Cuts at: 826 1045 2052 2369 2843 2920 3955 4199 4582 Size: 219 1007 317 474 77 1035 244 383 Cuts at: 4582 5717 6398 6589 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1035 1007 681 474 383 317 244 219 191 77 SfoI GGC'GCC Cuts at: 4142 4142 Size: 7234 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7234 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7234 SmaI CCC'GGG Cuts at: 1872 3794 4561 1872 Size: 1922 767 4545 Fragments arranged by size: 4545 1922 767 SmlI C'TyrA_G Cuts at: 820 1017 1943 2941 3266 5338 6239 6501 820 Size: 197 926 998 325 2072 901 262 1553 Fragments arranged by size: 2072 1553 998 926 901 325 262 197 SnaBI TAC'GTA Cuts at: 493 5025 493 Size: 4532 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7234 SphI G_CATG'C Cuts at: 1132 4592 1132 Size: 3460 3774 SrfI GCCC'GGGC Cuts at: 3794 3794 Size: 7234 SspI AAT'ATT Cuts at: 5 52 5158 5 Size: 47 5106 2081 Fragments arranged by size: 5106 2081 47 StuI AGG'CCT Cuts at: 1517 2195 2450 2523 2541 3002 1517 Size: 678 255 73 18 461 5749 Fragments arranged by size: 5749 678 461 255 73 18 StyI C'CwwG_G Cuts at: 513 1065 3157 4673 513 Size: 552 2092 1516 3074 Fragments arranged by size: 3074 2092 1516 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2616 2690 3231 3611 5261 5278 5431 Size: 1255 1036 74 541 380 1650 17 153 Cuts at: 5431 5616 6061 325 Size: 185 445 1498 Fragments arranged by size: 1650 1498 1255 1036 541 445 380 185 153 74 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2718 5480 7192 Size: 274 80 33 51 1443 741 2762 1712 Cuts at: 7192 96 Size: 138 Fragments arranged by size: 2762 1712 1443 741 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2591 2623 4176 4208 7194 7226 2591 Size: 32 1553 32 2986 32 2599 Fragments arranged by size: 2986 2599 1553 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7234 XbaI T'CTAG_A Cuts at: 2084 4570 2084 Size: 2486 4748 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4289 4360 4289 Size: 71 7163 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7234 XmaI C'CCGG_G Cuts at: 1870 3792 4559 1870 Size: 1922 767 4545 Fragments arranged by size: 4545 1922 767 XmnI GAAnn'nnTTC Cuts at: 5363 5363 Size: 7234 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5042 276 Size: 53 83 186 936 3508 2468 Fragments arranged by size: 3508 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI PacI PspXI PsrI RsrII SacII SexAI SfiI SwaI