(Circular) (Six-base) MAPSORT of: pFN21AA1539.seq Check: 9499 from: 1 to: 6319 pFN21AA1539 6319 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2877 2877 Size: 6319 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2145 3662 1937 Size: 208 1517 4594 Fragments arranged by size: 4594 1517 208 Acc65I G'GTAC_C Cuts at: 2177 3640 2177 Size: 1463 4856 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 2304 2400 2972 4382 5766 6034 1632 Size: 629 43 96 572 1410 1384 268 1917 Fragments arranged by size: 1917 1410 1384 629 572 268 96 43 AflII C'TTAA_G Cuts at: 820 1017 3493 820 Size: 197 2476 3646 Fragments arranged by size: 3646 2476 197 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2483 2598 1184 Size: 1299 115 4905 Fragments arranged by size: 4905 1299 115 AlwNI CAG_nnn'CTG Cuts at: 2481 2612 4062 5634 2481 Size: 131 1450 1572 3166 Fragments arranged by size: 3166 1572 1450 131 ApaI G_GGCC'C Cuts at: 2086 2200 2883 2086 Size: 114 683 5522 Fragments arranged by size: 5522 683 114 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 3628 3854 4006 6115 1493 Size: 2135 226 152 2109 1697 Fragments arranged by size: 2135 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2138 2232 2678 3644 1870 Size: 73 195 94 446 966 4545 Fragments arranged by size: 4545 966 446 195 94 73 AvrII C'CTAG_G Cuts at: 2642 2642 Size: 6319 BamHI G'GATC_C Cuts at: 2804 3649 4016 2804 Size: 845 367 5107 Fragments arranged by size: 5107 845 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2177 2796 3278 3400 3640 Size: 325 217 627 390 619 482 122 240 Cuts at: 3640 5095 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 619 482 390 325 240 217 122 BanII G_rGCy'C Cuts at: 1391 2086 2200 2307 2606 2883 2944 2963 3002 Size: 695 114 107 299 277 61 19 39 Cuts at: 3002 3479 3558 3638 1391 Size: 477 79 80 4072 Fragments arranged by size: 4072 695 477 299 277 114 107 80 79 61 39 19 BbeI G_GCGC'C Cuts at: 2800 2800 Size: 6319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5348 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4510 4544 1903 Size: 34 50 34 2489 34 3678 Fragments arranged by size: 3678 2489 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2164 4211 5421 2164 Size: 2047 1210 3062 Fragments arranged by size: 3062 2047 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4930 136 Size: 107 122 71 631 605 3258 1525 Fragments arranged by size: 3258 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 2957 2996 3736 2957 Size: 39 740 5540 Fragments arranged by size: 5540 740 39 Bme1580I G_kGCm'C Cuts at: 1790 2086 2200 2673 2883 3270 3281 4380 5536 Size: 296 114 473 210 387 11 1099 1156 Cuts at: 5536 1790 Size: 2573 Fragments arranged by size: 2573 1156 1099 473 387 296 210 114 11 BmgBI CAC'GTC Cuts at: 1318 3157 1318 Size: 1839 4480 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2491 2600 3000 Size: 416 53 83 399 24 1074 109 400 Cuts at: 3000 4050 5008 442 Size: 1050 958 1753 Fragments arranged by size: 1753 1074 1050 958 416 400 399 109 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2052 2072 2446 2526 2766 2881 3560 4979 Size: 813 20 374 80 240 115 679 1419 Cuts at: 4979 1239 Size: 2579 Fragments arranged by size: 2579 1419 813 679 374 240 115 80 20 Bpu10I CC'TnA_GC Cuts at: 3484 3484 Size: 6319 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4444 5309 5607 4444 Size: 865 298 5156 Fragments arranged by size: 5156 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4982 882 Size: 617 3483 2219 Fragments arranged by size: 3483 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2014 4110 493 Size: 711 810 2096 2702 Fragments arranged by size: 2702 2096 810 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2797 4126 4508 5143 Size: 53 83 186 936 1264 1329 382 635 Cuts at: 5143 275 Size: 1451 Fragments arranged by size: 1451 1329 1264 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2976 3471 4752 5150 5424 5571 5982 2976 Size: 495 1281 398 274 147 411 3313 Fragments arranged by size: 3313 1281 495 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2471 2501 3241 3271 1178 Size: 30 165 30 1068 30 740 30 4226 Fragments arranged by size: 4226 1068 740 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3428 3434 1186 Size: 2242 6 4071 Fragments arranged by size: 4071 2242 6 BseYI C'CCAG_C Cuts at: 2116 2257 2329 2612 3049 3150 5522 6018 2116 Size: 141 72 283 437 101 2372 496 2417 Fragments arranged by size: 2417 2372 496 437 283 141 101 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 2537 3443 1163 Size: 573 300 501 906 4039 Fragments arranged by size: 4039 906 573 501 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2685 3479 4380 4465 5536 2685 Size: 794 901 85 1071 3468 Fragments arranged by size: 3468 1071 901 794 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 BsmBI CGTCTCn'nnnn_ Cuts at: 2034 2034 Size: 6319 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2200 2307 2606 2673 2685 2883 Size: 399 296 114 107 299 67 12 198 Cuts at: 2883 2944 2963 3002 3270 3281 3479 3558 3638 Size: 61 19 39 268 11 198 79 80 Cuts at: 3638 4380 4465 5536 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 299 296 268 198 198 114 107 85 80 79 67 61 39 19 12 11 BspEI T'CCGG_A Cuts at: 2976 3471 5982 2976 Size: 495 2511 3313 Fragments arranged by size: 3313 2511 495 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2877 3674 844 Size: 2033 797 3489 Fragments arranged by size: 3489 2033 797 BsrBI CCG'CTC Cuts at: 1394 1823 3483 4206 1394 Size: 429 1660 723 3507 Fragments arranged by size: 3507 1660 723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2629 3068 4814 4988 65 Size: 1183 1381 439 1746 174 1396 Fragments arranged by size: 1746 1396 1381 1183 439 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4963 5150 1700 Size: 110 3153 187 2869 Fragments arranged by size: 3153 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6319 BstBI TT'CG_AA Cuts at: 2968 5123 2968 Size: 2155 4164 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstYI r'GATC_y Cuts at: 1972 2494 2804 3232 3649 4016 4401 4418 5870 Size: 522 310 428 417 367 385 17 1452 Cuts at: 5870 5956 5968 6314 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 522 428 417 385 367 346 310 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3464 3464 Size: 6319 BtgI C'CryG_G Cuts at: 513 1065 2365 2919 513 Size: 552 1300 554 3913 Fragments arranged by size: 3913 1300 554 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3371 487 Size: 683 56 2145 3435 Fragments arranged by size: 3435 2145 683 56 BtsI GCAGTG_nn' Cuts at: 2658 3835 4629 4649 2658 Size: 1177 794 20 4328 Fragments arranged by size: 4328 1177 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2343 3097 3766 Size: 54 1425 50 275 18 513 754 669 Cuts at: 3766 4655 6284 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 754 669 513 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 2769 4249 1353 Size: 884 532 1480 3423 Fragments arranged by size: 3423 1480 884 532 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2036 2941 3503 4906 5280 5426 Size: 1064 138 447 905 562 1403 374 146 Cuts at: 5426 387 Size: 1280 Fragments arranged by size: 1403 1280 1064 905 562 447 374 146 138 EcoICRI GAG'CTC Cuts at: 3477 3477 Size: 6319 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2072 2261 2304 2400 2446 2526 Size: 393 420 20 189 43 96 46 80 Cuts at: 2526 2766 2881 2972 3560 4382 4979 5766 6034 Size: 240 115 91 588 822 597 787 268 Cuts at: 6034 1239 Size: 1524 Fragments arranged by size: 1524 822 787 597 588 420 393 268 240 189 115 96 91 80 46 43 20 EcoNI CCTnn'n_nnAGG Cuts at: 2978 3247 2978 Size: 269 6050 EcoO109I rG'GnC_Cy Cuts at: 2082 2083 2196 2197 2228 2249 2421 2511 2711 Size: 1 113 1 31 21 172 90 200 Cuts at: 2711 3586 2082 Size: 875 4815 Fragments arranged by size: 4815 875 200 172 113 90 31 21 1 1 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 EcoRV GAT'ATC Cuts at: 3511 3511 Size: 6319 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 2800 5466 1678 Size: 1122 2666 2531 Fragments arranged by size: 2666 2531 1122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2471 2503 2750 2782 4960 Size: 32 135 32 899 32 247 32 2178 Cuts at: 4960 4992 5034 5066 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2178 899 247 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KasI G'GCGC_C Cuts at: 2796 2796 Size: 6319 KpnI G_GTAC'C Cuts at: 2181 3644 2181 Size: 1463 4856 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5433 5617 1305 Size: 4128 184 2007 Fragments arranged by size: 4128 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2345 3099 10 Size: 54 1425 343 513 754 3230 Fragments arranged by size: 3230 1425 754 513 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4277 4636 4795 518 Size: 648 3111 359 159 2042 Fragments arranged by size: 3111 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2551 3735 4412 5560 5805 1606 Size: 945 1184 677 1148 245 2120 Fragments arranged by size: 2120 1184 1148 945 677 245 NarI GG'CG_CC Cuts at: 2797 2797 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2919 513 Size: 552 1854 3913 Fragments arranged by size: 3913 1854 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 3358 3677 5222 1132 Size: 97 2129 319 1545 2229 Fragments arranged by size: 2229 2129 1545 319 97 PasI CC'CwG_GG Cuts at: 1348 2252 2515 2528 2809 1348 Size: 904 263 13 281 4858 Fragments arranged by size: 4858 904 281 263 13 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 2481 2612 3316 1108 Size: 1373 131 704 4111 Fragments arranged by size: 4111 1373 704 131 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PmlI CAC'GTG Cuts at: 2014 2014 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2228 2511 2228 Size: 283 6036 PshAI GACnn'nnGTC Cuts at: 1613 2509 2913 1613 Size: 896 404 5019 Fragments arranged by size: 5019 896 404 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspOMI G'GGCC_C Cuts at: 2082 2196 2879 2082 Size: 114 683 5522 Fragments arranged by size: 5522 683 114 PstI C_TGCA'G Cuts at: 830 3573 3671 830 Size: 2743 98 3478 Fragments arranged by size: 3478 2743 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 PvuII CAG'CTG Cuts at: 2551 2551 Size: 6319 SacI G_AGCT'C Cuts at: 3479 3479 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SanDI GG'GwC_CC Cuts at: 2228 2511 2228 Size: 283 6036 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SexAI A'CCwGG_T Cuts at: 2148 2148 Size: 6319 SfcI C'TryA_G Cuts at: 826 1045 2110 3569 3667 4802 5483 5674 826 Size: 219 1065 1459 98 1135 681 191 1471 Fragments arranged by size: 1471 1459 1135 1065 681 219 191 98 SfoI GGC'GCC Cuts at: 2798 2798 Size: 6319 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 2140 2234 3646 1872 Size: 268 94 1412 4545 Fragments arranged by size: 4545 1412 268 94 SmlI C'TyrA_G Cuts at: 820 1017 1943 3493 4423 5324 5586 820 Size: 197 926 1550 930 901 262 1553 Fragments arranged by size: 1553 1550 930 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 2991 1517 Size: 1474 4845 StyI C'CwwG_G Cuts at: 513 1065 2642 2919 3243 3270 3758 513 Size: 552 1577 277 324 27 488 3074 Fragments arranged by size: 3074 1577 552 488 324 277 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3398 3449 3596 4346 4363 4516 4701 Size: 1255 1818 51 147 750 17 153 185 Cuts at: 4701 5146 325 Size: 445 1498 Fragments arranged by size: 1818 1498 1255 750 445 185 153 147 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4565 6277 96 Size: 274 80 33 51 1443 2588 1712 138 Fragments arranged by size: 2588 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2212 2244 2325 2357 6279 6311 2212 Size: 32 81 32 3922 32 2220 Fragments arranged by size: 3922 2220 81 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2873 3313 2873 Size: 440 5879 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 2138 2232 3644 1870 Size: 268 94 1412 4545 Fragments arranged by size: 4545 1412 268 94 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI