(Circular) (Six-base) MAPSORT of: pFN21AA1497.seq Check: 5016 from: 1 to: 6853 pFN21AA1497 6853 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3659 4663 278 Size: 53 83 186 936 2123 1004 2468 Fragments arranged by size: 2468 2123 1004 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2419 2913 4125 4196 1937 Size: 482 494 1212 71 4594 Fragments arranged by size: 4594 1212 494 482 71 Acc65I G'GTAC_C Cuts at: 3449 3572 4174 3449 Size: 123 602 6128 Fragments arranged by size: 6128 602 123 AclI AA'CG_TT Cuts at: 1563 4980 5353 1563 Size: 3417 373 3063 Fragments arranged by size: 3417 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4916 6300 6568 1632 Size: 3284 1384 268 1917 Fragments arranged by size: 3284 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6656 AflIII A'CryG_T Cuts at: 1203 3497 4146 5752 6729 1203 Size: 2294 649 1606 977 1327 Fragments arranged by size: 2294 1606 1327 977 649 AgeI A'CCGG_T Cuts at: 5684 5684 Size: 6853 AhdI GACnn_n'nnGTC Cuts at: 5582 5582 Size: 6853 AleI CACnn'nnGTG Cuts at: 1166 2228 3533 1166 Size: 1062 1305 4486 Fragments arranged by size: 4486 1305 1062 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6853 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3255 3287 3255 Size: 32 6821 AlwNI CAG_nnn'CTG Cuts at: 3368 4596 6168 3368 Size: 1228 1572 4053 Fragments arranged by size: 4053 1572 1228 ApaLI G'TGCA_C Cuts at: 4910 6066 4910 Size: 1156 5697 ApoI r'AATT_y Cuts at: 1493 2121 2586 2619 2650 2794 2801 3372 4162 Size: 628 465 33 31 144 7 571 790 Cuts at: 4162 4388 4540 6649 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 790 628 571 465 226 152 144 33 31 7 AseI AT'TA_AT Cuts at: 160 1032 5407 160 Size: 872 4375 1606 Fragments arranged by size: 4375 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 AvaI C'yCGr_G Cuts at: 1870 1943 3531 4178 1870 Size: 73 1588 647 4545 Fragments arranged by size: 4545 1588 647 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3484 3517 3564 3597 3484 Size: 33 47 33 6740 Fragments arranged by size: 6740 47 33 33 BamHI G'GATC_C Cuts at: 4183 4550 4183 Size: 367 6486 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3225 3449 3572 4174 5629 Size: 325 217 627 1438 224 123 602 1455 Cuts at: 5629 618 Size: 1842 Fragments arranged by size: 1842 1455 1438 627 602 325 224 217 123 BanII G_rGCy'C Cuts at: 1391 2904 3186 4172 1391 Size: 1513 282 986 4072 Fragments arranged by size: 4072 1513 986 282 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2070 2267 3486 928 Size: 971 171 197 1219 4295 Fragments arranged by size: 4295 1219 971 197 171 BbvCI CC'TCA_GC Cuts at: 2429 2429 Size: 6853 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2236 2270 2903 2937 5044 5078 1903 Size: 34 299 34 633 34 2107 34 3678 Fragments arranged by size: 3678 2107 633 299 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3116 4745 5955 3116 Size: 1629 1210 4014 Fragments arranged by size: 4014 1629 1210 BclI T'GATC_A Cuts at: 1548 3877 1548 Size: 2329 4524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5464 136 Size: 107 122 71 631 605 3792 1525 Fragments arranged by size: 3792 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6848 6848 Size: 6853 BlpI GC'TnA_GC Cuts at: 3357 4270 3357 Size: 913 5940 Bme1580I G_kGCm'C Cuts at: 1790 3228 4914 6070 1790 Size: 1438 1686 1156 2573 Fragments arranged by size: 2573 1686 1438 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6853 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2199 2888 3388 Size: 416 53 83 399 24 782 689 500 Cuts at: 3388 3722 4584 5542 442 Size: 334 862 958 1753 Fragments arranged by size: 1753 958 862 782 689 500 416 399 334 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6853 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5513 1239 Size: 4274 2579 Bpu10I CC'TnA_GC Cuts at: 2429 2429 Size: 6853 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2747 4978 5843 6141 2747 Size: 2231 865 298 3459 Fragments arranged by size: 3459 2231 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5516 882 Size: 617 4017 2219 Fragments arranged by size: 4017 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4644 493 Size: 711 3440 2702 Fragments arranged by size: 3440 2702 711 BsaBI GATnn'nnATC Cuts at: 3104 6847 3104 Size: 3743 3110 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3656 4660 5042 5677 Size: 53 83 186 936 2123 1004 382 635 Cuts at: 5677 275 Size: 1451 Fragments arranged by size: 2123 1451 1004 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5286 5684 5958 6105 6516 5286 Size: 398 274 147 411 5623 Fragments arranged by size: 5623 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2513 2543 3173 3203 3255 Size: 30 165 30 1110 30 630 30 52 Cuts at: 3255 3285 1178 Size: 30 4746 Fragments arranged by size: 4746 1110 630 165 52 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6853 BseYI C'CCAG_C Cuts at: 2047 2361 6056 6552 2047 Size: 314 3695 496 2348 Fragments arranged by size: 3695 2348 496 314 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6280 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2917 4303 5064 6092 1540 Size: 275 186 916 1386 761 1028 2301 Fragments arranged by size: 2301 1386 1028 916 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2904 4914 4999 6070 2904 Size: 2010 85 1071 3687 Fragments arranged by size: 3687 2010 1071 85 BsmI GAATG_Cn' Cuts at: 3058 4364 4457 3058 Size: 1306 93 5454 Fragments arranged by size: 5454 1306 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2904 3186 3228 4172 4914 4999 6070 Size: 399 1114 282 42 944 742 85 1071 Cuts at: 6070 1391 Size: 2174 Fragments arranged by size: 2174 1114 1071 944 742 399 282 85 42 BspEI T'CCGG_A Cuts at: 6516 6516 Size: 6853 BspHI T'CATG_A Cuts at: 2592 3651 4742 6472 2592 Size: 1059 1091 1730 2973 Fragments arranged by size: 2973 1730 1091 1059 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4208 844 Size: 3364 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4740 1394 Size: 429 2917 3507 Fragments arranged by size: 3507 2917 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2185 3945 5348 5522 65 Size: 1183 937 1760 1403 174 1396 Fragments arranged by size: 1760 1403 1396 1183 937 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5497 5684 1700 Size: 110 3687 187 2869 Fragments arranged by size: 3687 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6853 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6853 BssSI C'ACGA_G Cuts at: 4913 5925 4913 Size: 1012 5841 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6853 BstBI TT'CG_AA Cuts at: 5657 5657 Size: 6853 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6853 BstYI r'GATC_y Cuts at: 1972 4183 4550 4935 4952 6404 6490 6502 6848 Size: 2211 367 385 17 1452 86 12 346 Cuts at: 6848 1972 Size: 1977 Fragments arranged by size: 2211 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3122 3122 Size: 6853 BtgI C'CryG_G Cuts at: 513 1065 2004 3351 513 Size: 552 939 1347 4015 Fragments arranged by size: 4015 1347 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2239 487 Size: 683 56 1013 5101 Fragments arranged by size: 5101 1013 683 56 BtsI GCAGTG_nn' Cuts at: 3870 4369 5163 5183 3870 Size: 499 794 20 5540 Fragments arranged by size: 5540 794 499 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6853 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2152 2187 529 Size: 35 1588 35 5195 Fragments arranged by size: 5195 1588 35 35 DraI TTT'AAA Cuts at: 4157 4157 Size: 6853 DraIII CAC_nnn'GTG Cuts at: 3232 3232 Size: 6853 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3823 5860 809 Size: 507 2507 2037 1802 Fragments arranged by size: 2507 2037 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4300 5189 6818 Size: 54 1425 50 275 18 2470 889 1629 Cuts at: 6818 8 Size: 43 Fragments arranged by size: 2470 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4300 1537 Size: 275 2488 4090 Fragments arranged by size: 4090 2488 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4027 4783 1353 Size: 2674 756 3423 Fragments arranged by size: 3423 2674 756 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5440 5814 5960 387 Size: 1064 138 3851 374 146 1280 Fragments arranged by size: 3851 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2902 2902 Size: 6853 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4916 5513 6300 6568 1239 Size: 393 3284 597 787 268 1524 Fragments arranged by size: 3284 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3558 3724 3558 Size: 166 6687 EcoRI G'AATT_C Cuts at: 2619 4162 4540 2619 Size: 1543 378 4932 Fragments arranged by size: 4932 1543 378 EcoRV GAT'ATC Cuts at: 3282 3319 3282 Size: 37 6816 FspI TGC'GCA Cuts at: 5359 5359 Size: 6853 HaeII r_GCGC'y Cuts at: 1678 6000 1678 Size: 4322 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2720 2752 3173 3205 5494 Size: 32 135 32 1148 32 421 32 2289 Cuts at: 5494 5526 5568 5600 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2289 1148 421 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2148 2914 3553 4126 4197 4443 Size: 988 281 210 766 639 573 71 246 Cuts at: 4443 669 Size: 3079 Fragments arranged by size: 3079 988 766 639 573 281 246 210 71 HindIII A'AGCT_T Cuts at: 748 2659 3025 748 Size: 1911 366 4576 Fragments arranged by size: 4576 1911 366 HpaI GTT'AAC Cuts at: 3553 4443 3553 Size: 890 5963 KpnI G_GTAC'C Cuts at: 3453 3576 4178 3453 Size: 123 602 6128 Fragments arranged by size: 6128 602 123 MfeI C'AATT_G Cuts at: 2039 4452 2039 Size: 2413 4440 MluI A'CGCG_T Cuts at: 6729 6729 Size: 6853 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2556 3180 5967 6151 1305 Size: 1251 624 2787 184 2007 Fragments arranged by size: 2787 2007 1251 624 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5031 Fragments arranged by size: 5031 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 3533 3623 3950 4811 5170 5329 Size: 648 1062 1305 90 327 861 359 159 Cuts at: 5329 518 Size: 2042 Fragments arranged by size: 2042 1305 1062 861 648 359 327 159 90 MspA1I CmG'CkG Cuts at: 1606 4269 4946 6094 6339 1606 Size: 2663 677 1148 245 2120 Fragments arranged by size: 2663 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3351 513 Size: 552 939 1347 4015 Fragments arranged by size: 4015 1347 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6853 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6853 NotI GC'GGCC_GC Cuts at: 4300 4300 Size: 6853 NruI TCG'CGA Cuts at: 2258 2258 Size: 6853 NspI r_CATG'y Cuts at: 1132 1229 3501 4150 4211 5756 1132 Size: 97 2272 649 61 1545 2229 Fragments arranged by size: 2272 2229 1545 649 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6853 PciI A'CATG_T Cuts at: 3497 4146 5752 3497 Size: 649 1606 4598 Fragments arranged by size: 4598 1606 649 PflMI CCAn_nnn'nTGG Cuts at: 1108 2995 1108 Size: 1887 4966 PfoI T'CCnGG_A Cuts at: 2695 3249 3629 2695 Size: 554 380 5919 Fragments arranged by size: 5919 554 380 PmeI GTTT'AAAC Cuts at: 4157 4157 Size: 6853 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3255 3287 4922 4954 6435 6467 3255 Size: 32 1635 32 1481 32 3641 Fragments arranged by size: 3641 1635 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3558 3724 3558 Size: 166 6687 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6853 PsiI TTA'TAA Cuts at: 4423 4423 Size: 6853 PstI C_TGCA'G Cuts at: 830 2280 3765 3907 4105 4205 830 Size: 1450 1485 142 198 100 3478 Fragments arranged by size: 3478 1485 1450 198 142 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6853 SacI G_AGCT'C Cuts at: 2904 2904 Size: 6853 SalI G'TCGA_C Cuts at: 1936 2912 4124 4195 1936 Size: 976 1212 71 4594 Fragments arranged by size: 4594 1212 976 71 SanDI GG'GwC_CC Cuts at: 3558 3724 3558 Size: 166 6687 SbfI CC_TGCA'GG Cuts at: 4205 4205 Size: 6853 ScaI AGT'ACT Cuts at: 5101 6813 5101 Size: 1712 5141 SexAI A'CCwGG_T Cuts at: 3228 3228 Size: 6853 SfcI C'TryA_G Cuts at: 826 1045 2276 3761 3903 4101 4201 5336 6017 Size: 219 1231 1485 142 198 100 1135 681 Cuts at: 6017 6208 826 Size: 191 1471 Fragments arranged by size: 1485 1471 1231 1135 681 219 198 191 142 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6853 SmaI CCC'GGG Cuts at: 1872 4180 1872 Size: 2308 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2726 4957 5858 6120 820 Size: 197 926 783 2231 901 262 1553 Fragments arranged by size: 2231 1553 926 901 783 262 197 SnaBI TAC'GTA Cuts at: 493 4644 493 Size: 4151 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6853 SphI G_CATG'C Cuts at: 1132 4211 1132 Size: 3079 3774 SspI AAT'ATT Cuts at: 5 52 3805 4777 5 Size: 47 3753 972 2081 Fragments arranged by size: 3753 2081 972 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6853 StyI C'CwwG_G Cuts at: 513 1065 2004 3351 3453 3517 4292 513 Size: 552 939 1347 102 64 775 3074 Fragments arranged by size: 3074 1347 939 775 552 102 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2590 4880 4897 5050 5235 5680 325 Size: 1255 1010 2290 17 153 185 445 1498 Fragments arranged by size: 2290 1498 1255 1010 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3890 3954 5099 Size: 274 80 33 51 1443 1913 64 1145 Cuts at: 5099 6811 96 Size: 1712 138 Fragments arranged by size: 1913 1712 1443 1145 274 138 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2513 2545 2677 2709 6813 6845 2513 Size: 32 132 32 4104 32 2521 Fragments arranged by size: 4104 2521 132 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6853 XbaI T'CTAG_A Cuts at: 4189 4189 Size: 6853 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6853 XmaI C'CCGG_G Cuts at: 1870 4178 1870 Size: 2308 4545 XmnI GAAnn'nnTTC Cuts at: 4982 4982 Size: 6853 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3657 4661 276 Size: 53 83 186 936 2123 1004 2468 Fragments arranged by size: 2468 2123 1004 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI