Restriction map for
pFN21AA1475
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AvrII BamHI BcgI BglII
BlnI BsaBI BsiWI BspDI BspHI
BspLU11I BsrDI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NotI NruI NsiI NspI
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SgrAI SnaBI SpeI SphI SspI
SwaI TfiI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 2146 GTCTAC GT^[AC][GT]AC
AccII 2131 CGCG CG^CG
AccIII 555 TCCGGA T^CCGGA
AcsI 1084 AAATTT [GA]^AATT[CT]
AflII 2425 CTTAAG C^TTAAG
Alw44I 1038 GTGCAC G^TGCAC
AocI 2233 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2888 AGCGCT AGC^GCT
ApaLI 1038 GTGCAC G^TGCAC
ApoI 1084 AAATTT [GA]^AATT[CT]
AspI 1267 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2252 TGCGCA TGC^GCA
BanI 899 GGCGCC G^G[CT][GA]CC
BbeI 899 GGCGCC GGCGC^C
BbsI 1156 GTCTTC GAAGAC(2/6)
BciVI 219 GGATAC GTATCC(6/5)
BclI 1682 TGATCA T^GATCA
BfrI 2425 CTTAAG C^TTAAG
BlpI 915 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 915 GCTGAGC GC^T[ACGT]AGC
BpuAI 1156 GTCTTC GAAGAC(2/6)
BsaI 2504 GAGACC GGTCTC(1/5)
BsaWI 555 TCCGGA [AT]^CCGG[AT]
BseAI 555 TCCGGA T^CCGGA
BsmBI 183 CGTCTC CGTCTC(1/5)
Bsp1407I 2307 TGTACA T^GTACA
BspEI 555 TCCGGA T^CCGGA
BsrGI 2307 TGTACA T^GTACA
BssSI 1726 CACGAG CACGAG(-5/-1)
BstBI 1568 TTCGAA TT^CGAA
BstUI 2131 CGCG CG^CG
BstYI 558 GGATCT [GA]^GATC[CT]
Bsu36I 2233 CCTGAGG CC^T[ACGT]AGG
CelII 915 GCTGAGC GC^T[ACGT]AGC
Eco47III 2888 AGCGCT AGC^GCT
Eco81I 2233 CCTGAGG CC^T[ACGT]AGG
FbaI 1682 TGATCA T^GATCA
FseI 2020 GGCCGGCC GGCCGG^CC
FspI 2252 TGCGCA TGC^GCA
KasI 899 GGCGCC G^GCGCC
MflI 558 GGATCT [GA]^GATC[CT]
MroI 555 TCCGGA T^CCGGA
MseI 2426 TTAA T^TAA
MslI 608 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2131 CGCG CG^CG
NarI 899 GGCGCC GG^CGCC
NcoI 2318 CCATGG C^CATGG
NdeI 2614 CATATG CA^TATG
NheI 2332 GCTAGC G^CTAGC
NspV 1568 TTCGAA TT^CGAA
PflFI 1267 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1779 GCTCTTC GCTCTTC(1/4)
SfoI 899 GGCGCC GGC^GCC
SfuI 1568 TTCGAA TT^CGAA
SspBI 2307 TGTACA T^GTACA
Tru9I 2426 TTAA T^TAA
Tth111I 1267 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 558 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 3 GGCGTC G[GA]^CG[CT]C
899 GGCGCC
ApaI 636 GGGCCC GGGCC^C
2776 GGGCCC
Asp700I 1215 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1561 GAATGCTTTC
BbvCI 1578 CCTCAGC CCTCAGC(-5/-2)
715 GCTGAGG
BsaHI 3 GGCGTC G[GA]^CG[CT]C
899 GGCGCC
BsgI 2222 GTGCAG GTGCAG(16/14)
2290 GTGCAG
BsmI 99 GAATGC GAATGC(1/-1)
1561 GAATGC
BstXI 260 CCAACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
785 CCAGCAACCTGG
Hin1I 3 GGCGTC G[GA]^CG[CT]C
899 GGCGCC
PpuMI 359 AGGTCCT [GA]G^G[AT]CC[CT]
2153 AGGTCCT
SacI 517 GAGCTC GAGCT^C
1489 GAGCTC
SbfI 2190 CCTGCAGG CCTGCA^GG
2508 CCTGCAGG
SfiI 1278 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2742 GGCCAAGGAGGCC
Sse8387I 2190 CCTGCAGG CCTGCA^GG
2508 CCTGCAGG
StuI 1730 AGGCCT AGG^CCT
2429 AGGCCT
Tsp509I 1085 AATT ^AATT
2249 AATT
XmnI 1215 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1561 GAATGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanII
BbrPI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PmaCI PmlI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XmaCI XmaI