Restriction map for
pFN21AA1475
(Linear) (Six-base) MAPSORT of: KIAA1475.seq Check: 4198 from: 1 to: 2919
KIAA1475.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 341 1044 1911 2184 2572 2919
Size: 341 703 867 273 388 347
Fragments arranged by size:
867 703 388 347 341 273
AccI GT'mk_AC
Cuts at: 0 2147 2919
Size: 2147 772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1117 2290 2919
Size: 1117 1173 629
Fragments arranged by size:
1173 1117 629
AfeI AGC'GCT
Cuts at: 0 2890 2919
Size: 2890 29
AflII C'TTAA_G
Cuts at: 0 2425 2919
Size: 2425 494
AleI CACnn'nnGTG
Cuts at: 0 612 2919
Size: 612 2307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 630 2919
Size: 630 2289
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 2919
Size: 19 32 2868
Fragments arranged by size:
2868 32 19
AlwNI CAG_nnn'CTG
Cuts at: 0 610 2454 2517 2919
Size: 610 1844 63 402
Fragments arranged by size:
1844 610 402 63
ApaI G_GGCC'C
Cuts at: 0 640 2780 2919
Size: 640 2140 139
Fragments arranged by size:
2140 640 139
ApaLI G'TGCA_C
Cuts at: 0 1038 2919
Size: 1038 1881
ApoI r'AATT_y
Cuts at: 0 1084 2919
Size: 1084 1835
AvaI C'yCGr_G
Cuts at: 0 87 842 1184 1280 2738 2919
Size: 87 755 342 96 1458 181
Fragments arranged by size:
1458 755 342 181 96 87
BanI G'GyrC_C
Cuts at: 0 899 2919
Size: 899 2020
BanII G_rGCy'C
Cuts at: 0 260 521 640 740 1493 1515 2546 2734
Size: 260 261 119 100 753 22 1031 188
Cuts at: 2734 2780 2919
Size: 46 139
Fragments arranged by size:
1031 753 261 260 188 139 119 100
46 22
BbeI G_GCGC'C
Cuts at: 0 903 2919
Size: 903 2016
BbsI GAAGACnn'nnnn_
Cuts at: 0 1149 2919
Size: 1149 1770
BbvCI CC'TCA_GC
Cuts at: 0 716 1579 2919
Size: 716 863 1340
Fragments arranged by size:
1340 863 716
BciVI GTATCCnnnnn_n'
Cuts at: 0 213 2919
Size: 213 2706
BclI T'GATC_A
Cuts at: 0 1682 2919
Size: 1682 1237
BglI GCCn_nnn'nGGC
Cuts at: 0 1285 1618 2531 2749 2919
Size: 1285 333 913 218 170
Fragments arranged by size:
1285 913 333 218 170
BlpI GC'TnA_GC
Cuts at: 0 916 2919
Size: 916 2003
Bme1580I G_kGCm'C
Cuts at: 0 125 457 640 1042 2490 2780 2919
Size: 125 332 183 402 1448 290 139
Fragments arranged by size:
1448 402 332 290 183 139 125
BmrI ACTGGGnnnn_n'
Cuts at: 0 460 987 2919
Size: 460 527 1932
Fragments arranged by size:
1932 527 460
BmtI G_CTAG'C
Cuts at: 0 2336 2919
Size: 2336 583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 171 1099 1339 1402 2068 2704 2919
Size: 130 41 928 240 63 666 636 215
Fragments arranged by size:
928 666 636 240 215 130 63 41
Bpu10I CC'TnA_GC
Cuts at: 0 346 716 1498 1579 2919
Size: 346 370 782 81 1340
Fragments arranged by size:
1340 782 370 346 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1357 2074 2332 2779 2919
Size: 643 714 717 258 447 140
Fragments arranged by size:
717 714 643 447 258 140
BsaI GGTCTCn'nnnn_
Cuts at: 0 2498 2919
Size: 2498 421
BsaAI yAC'GTr
Cuts at: 0 267 369 582 1170 2286 2919
Size: 267 102 213 588 1116 633
Fragments arranged by size:
1116 633 588 267 213 102
BsaHI Gr'CG_yC
Cuts at: 0 4 900 2919
Size: 4 896 2019
Fragments arranged by size:
2019 896 4
BsaWI w'CCGG_w
Cuts at: 0 555 2919
Size: 555 2364
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1021 1051 2919
Size: 1021 30 1868
Fragments arranged by size:
1868 1021 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 937 1107 1375 1411 1753 2185 2251 2701
Size: 937 170 268 36 342 432 66 450
Cuts at: 2701 2911 2914 2919
Size: 210 3 5
Fragments arranged by size:
937 450 432 342 268 210 170 66
36 5 3
BseYI C'CCAG_C
Cuts at: 0 104 273 784 1775 1965 2056 2489 2703
Size: 104 169 511 991 190 91 433 214
Cuts at: 2703 2721 2779 2885 2919
Size: 18 58 106 34
Fragments arranged by size:
991 511 433 214 190 169 106 104
91 58 34 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2243 2311 2919
Size: 2243 68 608
Fragments arranged by size:
2243 608 68
BsiEI CG_ry'CG
Cuts at: 0 47 2026 2868 2872 2919
Size: 47 1979 842 4 47
Fragments arranged by size:
1979 842 47 47 4
BsiHKAI G_wGCw'C
Cuts at: 0 521 619 1042 1493 1757 2919
Size: 521 98 423 451 264 1162
Fragments arranged by size:
1162 521 451 423 264 98
BsmI GAATG_Cn'
Cuts at: 0 105 1567 2919
Size: 105 1462 1352
Fragments arranged by size:
1462 1352 105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 189 2919
Size: 189 2730
Bsp1286I G_dGCh'C
Cuts at: 0 125 260 457 521 619 640 740 1042
Size: 125 135 197 64 98 21 100 302
Cuts at: 1042 1493 1515 1757 2490 2546 2734 2780 2919
Size: 451 22 242 733 56 188 46 139
Fragments arranged by size:
733 451 302 242 197 188 139 135
125 100 98 64 56 46 22 21
BspEI T'CCGG_A
Cuts at: 0 555 2919
Size: 555 2364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 1044 1911 2006 2184 2237 2516 2572
Size: 341 703 867 95 178 53 279 56
Cuts at: 2572 2919
Size: 347
Fragments arranged by size:
867 703 347 341 279 178 95 56
53
BsrBI CCG'CTC
Cuts at: 0 41 633 1149 1251 1701 2858 2919
Size: 41 592 516 102 450 1157 61
Fragments arranged by size:
1157 592 516 450 102 61 41
BsrFI r'CCGG_y
Cuts at: 0 1502 1856 2021 2867 2919
Size: 1502 354 165 846 52
Fragments arranged by size:
1502 846 354 165 52
BsrGI T'GTAC_A
Cuts at: 0 2307 2919
Size: 2307 612
BssSI C'ACGA_G
Cuts at: 0 1726 2919
Size: 1726 1193
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1059 2289 2517 2646 2919
Size: 1059 1230 228 129 273
Fragments arranged by size:
1230 1059 273 228 129
BstBI TT'CG_AA
Cuts at: 0 1569 2919
Size: 1569 1350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 792 2919
Size: 267 525 2127
Fragments arranged by size:
2127 525 267
BstYI r'GATC_y
Cuts at: 0 558 2919
Size: 558 2361
Bsu36I CC'TnA_GG
Cuts at: 0 2234 2919
Size: 2234 685
BtgI C'CryG_G
Cuts at: 0 2016 2177 2318 2768 2919
Size: 2016 161 141 450 151
Fragments arranged by size:
2016 450 161 151 141
BtsI GCAGTG_nn'
Cuts at: 0 223 1040 1465 1685 2224 2919
Size: 223 817 425 220 539 695
Fragments arranged by size:
817 695 539 425 223 220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2116 2151 2919
Size: 2116 35 768
Fragments arranged by size:
2116 768 35
DraIII CAC_nnn'GTG
Cuts at: 0 1107 1668 2076 2289 2919
Size: 1107 561 408 213 630
Fragments arranged by size:
1107 630 561 408 213
EaeI y'GGCC_r
Cuts at: 0 44 394 1229 1430 1748 2019 2023 2121
Size: 44 350 835 201 318 271 4 98
Cuts at: 2121 2210 2610 2819 2869 2919
Size: 89 400 209 50 50
Fragments arranged by size:
835 400 350 318 271 209 201 98
89 50 50 44 4
EagI C'GGCC_G
Cuts at: 0 44 2023 2869 2919
Size: 44 1979 846 50
Fragments arranged by size:
1979 846 50 44
EarI CTCTTCn'nnn_
Cuts at: 0 688 1786 2291 2465 2605 2919
Size: 688 1098 505 174 140 314
Fragments arranged by size:
1098 688 505 314 174 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 559 2919
Size: 559 2360
EcoICRI GAG'CTC
Cuts at: 0 519 1491 2919
Size: 519 972 1428
Fragments arranged by size:
1428 972 519
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 171 1099 1117 1339 1402 2068 2290
Size: 130 41 928 18 222 63 666 222
Cuts at: 2290 2704 2919
Size: 414 215
Fragments arranged by size:
928 666 414 222 222 215 130 63
41 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 214 2233 2638 2919
Size: 214 2019 405 281
Fragments arranged by size:
2019 405 281 214
EcoO109I rG'GnC_Cy
Cuts at: 0 360 472 636 637 955 1533 1743 2154
Size: 360 112 164 1 318 578 210 411
Cuts at: 2154 2776 2919
Size: 622 143
Fragments arranged by size:
622 578 411 360 318 210 164 143
112 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 556 1581 2919
Size: 556 1025 1338
Fragments arranged by size:
1338 1025 556
FseI GG_CCGG'CC
Cuts at: 0 2025 2919
Size: 2025 894
FspI TGC'GCA
Cuts at: 0 2254 2919
Size: 2254 665
HaeII r_GCGC'y
Cuts at: 0 681 903 2892 2919
Size: 681 222 1989 27
Fragments arranged by size:
1989 681 222 27
KasI G'GCGC_C
Cuts at: 0 899 2919
Size: 899 2020
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 2919
Size: 418 2501
MscI TGG'CCA
Cuts at: 0 1231 1432 2123 2212 2612 2919
Size: 1231 201 691 89 400 307
Fragments arranged by size:
1231 691 400 307 201 89
MslI CAynn'nnrTG
Cuts at: 0 612 2919
Size: 612 2307
MspA1I CmG'CkG
Cuts at: 0 108 198 420 1095 1131 2460 2824 2919
Size: 108 90 222 675 36 1329 364 95
Fragments arranged by size:
1329 675 364 222 108 95 90 36
NaeI GCC'GGC
Cuts at: 0 1504 1858 2023 2919
Size: 1504 354 165 896
Fragments arranged by size:
1504 896 354 165
NarI GG'CG_CC
Cuts at: 0 900 2919
Size: 900 2019
NcoI C'CATG_G
Cuts at: 0 2318 2919
Size: 2318 601
NdeI CA'TA_TG
Cuts at: 0 2615 2919
Size: 2615 304
NgoMIV G'CCGG_C
Cuts at: 0 1502 1856 2021 2919
Size: 1502 354 165 898
Fragments arranged by size:
1502 898 354 165
NheI G'CTAG_C
Cuts at: 0 2332 2919
Size: 2332 587
PasI CC'CwG_GG
Cuts at: 0 723 2919
Size: 723 2196
PfoI T'CCnGG_A
Cuts at: 0 11 1076 1379 2447 2919
Size: 11 1065 303 1068 472
Fragments arranged by size:
1068 1065 472 303 11
PmlI CAC'GTG
Cuts at: 0 267 1170 2286 2919
Size: 267 903 1116 633
Fragments arranged by size:
1116 903 633 267
PpuMI rG'GwC_Cy
Cuts at: 0 360 2154 2919
Size: 360 1794 765
Fragments arranged by size:
1794 765 360
PspOMI G'GGCC_C
Cuts at: 0 636 2776 2919
Size: 636 2140 143
Fragments arranged by size:
2140 636 143
PstI C_TGCA'G
Cuts at: 0 1421 1520 2195 2419 2459 2513 2642 2696
Size: 1421 99 675 224 40 54 129 54
Cuts at: 2696 2919
Size: 223
Fragments arranged by size:
1421 675 224 223 129 99 54 54
40
PvuII CAG'CTG
Cuts at: 0 108 198 420 1131 2919
Size: 108 90 222 711 1788
Fragments arranged by size:
1788 711 222 108 90
SacI G_AGCT'C
Cuts at: 0 521 1493 2919
Size: 521 972 1426
Fragments arranged by size:
1426 972 521
SapI GCTCTTCn'nnn_
Cuts at: 0 1786 2919
Size: 1786 1133
SbfI CC_TGCA'GG
Cuts at: 0 2195 2513 2919
Size: 2195 318 406
Fragments arranged by size:
2195 406 318
SfcI C'TryA_G
Cuts at: 0 798 1417 1516 1896 2191 2415 2455 2477
Size: 798 619 99 380 295 224 40 22
Cuts at: 2477 2509 2638 2692 2919
Size: 32 129 54 227
Fragments arranged by size:
798 619 380 295 227 224 129 99
54 40 32 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1285 2749 2919
Size: 1285 1464 170
Fragments arranged by size:
1464 1285 170
SfoI GGC'GCC
Cuts at: 0 901 2919
Size: 901 2018
SmaI CCC'GGG
Cuts at: 0 844 1282 2740 2919
Size: 844 438 1458 179
Fragments arranged by size:
1458 844 438 179
SmlI C'TyrA_G
Cuts at: 0 622 1372 2089 2347 2425 2794 2919
Size: 622 750 717 258 78 369 125
Fragments arranged by size:
750 717 622 369 258 125 78
SrfI GCCC'GGGC
Cuts at: 0 2740 2919
Size: 2740 179
StuI AGG'CCT
Cuts at: 0 1732 2431 2919
Size: 1732 699 488
Fragments arranged by size:
1732 699 488
StyI C'CwwG_G
Cuts at: 0 495 1556 2318 2726 2744 2919
Size: 495 1061 762 408 18 175
Fragments arranged by size:
1061 762 495 408 175 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 776 1812 2919
Size: 776 1036 1107
Fragments arranged by size:
1107 1036 776
TatI w'GTAC_w
Cuts at: 0 1670 2307 2834 2919
Size: 1670 637 527 85
Fragments arranged by size:
1670 637 527 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 755 787 1021 1053 2919
Size: 755 32 234 32 1866
Fragments arranged by size:
1866 755 234 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1270 2919
Size: 1270 1649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 404 1941 2325 2919
Size: 404 1537 384 594
Fragments arranged by size:
1537 594 404 384
XmaI C'CCGG_G
Cuts at: 0 842 1280 2738 2919
Size: 842 438 1458 181
Fragments arranged by size:
1458 842 438 181
XmnI GAAnn'nnTTC
Cuts at: 0 1219 1565 2919
Size: 1219 346 1354
Fragments arranged by size:
1354 1219 346
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI FspI HaeII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI PasI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SapI
SbfI SfcI SfiI SfoI SmaI SmlI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AhdI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BfrBI BglII BmgBI
BplI BsaBI BsiWI BspHI BsrDI BssHII BstEII BstZ17I
BtgZI ClaI DraI DrdI EcoRI EcoRV FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI XbaI XhoI ZraI