Restriction map for
pFN21AA1370
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AvaI AviII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BsaBI BsaWI BseAI
BsiEI BsmI BsoBI BspDI BspEI
BspMI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRV
FauI FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SwaI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 1178 GACGTC GACGT^C
AcyI 1178 GACGTC G[GA]^CG[CT]C
AhdI 2302 GACTGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 310 GGGCCC GGGCC^C
AspEI 2302 GACTGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 818 GGACC G^G[AT]CC
AvrII 2634 CCTAGG C^CTAGG
BanI 825 GGCACC G^G[CT][GA]CC
BanII 310 GGGCCC G[GA]GC[CT]^C
BglII 3101 AGATCT A^GATCT
BlnI 2634 CCTAGG C^CTAGG
BlpI 1468 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1468 GCTCAGC GC^T[ACGT]AGC
BsaAI 1788 TACGTA [CT]AC^GT[GA]
BsaHI 1178 GACGTC G[GA]^CG[CT]C
BsaI 60 GGTCTC GGTCTC(1/5)
BsgI 678 GTGCAG GTGCAG(16/14)
BsiWI 3120 CGTACG C^GTACG
BsmBI 3116 GAGACG CGTCTC(1/5)
Bsp1407I 1954 TGTACA T^GTACA
BspHI 2580 TCATGA T^CATGA
BsrGI 1954 TGTACA T^GTACA
BstEII 409 GGTGACC G^GT[ACGT]ACC
BstPI 409 GGTGACC G^GT[ACGT]ACC
CelII 1468 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2302 GACTGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 409 GGTGACC G^GT[ACGT]ACC
EcoRI 716 GAATTC G^AATTC
Hin1I 1178 GACGTC G[GA]^CG[CT]C
MfeI 578 CAATTG C^AATTG
MunI 578 CAATTG C^AATTG
PflMI 904 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 875 CTGCAG CTGCA^G
RcaI 2580 TCATGA T^CATGA
SnaBI 1788 TACGTA TAC^GTA
SspBI 1954 TGTACA T^GTACA
StuI 2128 AGGCCT AGG^CCT
Van91I 904 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 547 GTAGAC GT^[AC][GT]AC
2310 GTCTAC
AlwNI 793 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
905 CAGGCACTG
AspHI 1480 GAGCAC G[AT]GC[AT]^C
2606 GTGCTC
BcnI 1227 CCGGG CC^[GC]GG
1511 CCCGG
BseRI 1352 CTCCTC GAGGAG(10/8)
49 GAGGAG
BsiHKAI 1480 GAGCAC G[AT]GC[AT]^C
2606 GTGCTC
BsmFI 931 GGGAC GGGAC(10/14)
2826 GTCCC
BstNI 904 CCAGG CC^[AT]GG
1437 CCAGG
Cac8I 192 GCTGGC GC[ACGT]^[ACGT]GC
2943 GCCAGC
DraI 1005 TTTAAA TTT^AAA
1702 TTTAAA
DsaI 821 CCATGG C^C[GA][CT]GG
927 CCATGG
EcoRII 904 CCAGG ^CC[AT]GG
1437 CCAGG
EcoT22I 359 ATGCAT ATGCA^T
1528 ATGCAT
HapII 1227 CCGG C^CGG
1512 CCGG
HgaI 432 GACGC GACGC(5/10)
795 GCGTC
HpaII 1227 CCGG C^CGG
1512 CCGG
MspA1I 878 CAGCTG C[AC]G^C[GT]G
1064 CAGCTG
MspI 1227 CCGG C^CGG
1512 CCGG
MvaI 904 CCAGG CC^[AT]GG
1437 CCAGG
NciI 1227 CCGGG CC^[GC]GG
1511 CCCGG
NcoI 821 CCATGG C^CATGG
927 CCATGG
NsiI 359 ATGCAT ATGCA^T
1528 ATGCAT
PvuII 878 CAGCTG CAG^CTG
1064 CAGCTG
SmlI 938 CTCAAG C^T[CT][GA]AG
1193 CTCAAG
XbaI 2533 TCTAGA T^CTAGA
3136 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
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AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI Bsp1286I BspLU11I
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstYI Cfr13I DdeI DpnII
EarI EcoNI EcoT14I Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI