(Circular) (Six-base) MAPSORT of: pFN21AA1370.seq Check: 9809 from: 1 to: 7933 pFN21AA1370 7933 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3187 5743 278 Size: 53 83 186 936 1651 2556 2468 Fragments arranged by size: 2556 2468 1651 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2553 4316 5276 1937 Size: 616 1763 960 4594 Fragments arranged by size: 4594 1763 960 616 Acc65I G'GTAC_C Cuts at: 5254 5254 Size: 7933 AclI AA'CG_TT Cuts at: 1563 6060 6433 1563 Size: 4497 373 3063 Fragments arranged by size: 4497 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4007 4848 5996 7380 7648 1632 Size: 2375 841 1148 1384 268 1917 Fragments arranged by size: 2375 1917 1384 1148 841 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7736 AflIII A'CryG_T Cuts at: 1203 3172 4142 4319 6832 7809 1203 Size: 1969 970 177 2513 977 1327 Fragments arranged by size: 2513 1969 1327 977 970 177 AgeI A'CCGG_T Cuts at: 6764 6764 Size: 7933 AhdI GACnn_n'nnGTC Cuts at: 4312 6662 4312 Size: 2350 5583 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7933 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7933 AlwNI CAG_nnn'CTG Cuts at: 2803 2915 5676 7248 2803 Size: 112 2761 1572 3488 Fragments arranged by size: 3488 2761 1572 112 ApaI G_GGCC'C Cuts at: 2319 2319 Size: 7933 ApaLI G'TGCA_C Cuts at: 5990 7146 5990 Size: 1156 6777 ApoI r'AATT_y Cuts at: 1493 2566 2721 3336 3589 4007 4645 5242 5468 Size: 1073 155 615 253 418 638 597 226 Cuts at: 5468 5620 7729 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1073 638 615 597 418 253 226 155 152 AseI AT'TA_AT Cuts at: 160 1032 6487 160 Size: 872 5455 1606 Fragments arranged by size: 5455 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7933 AvaI C'yCGr_G Cuts at: 1870 1943 5258 1870 Size: 73 3315 4545 Fragments arranged by size: 4545 3315 73 AvrII C'CTAG_G Cuts at: 4639 4639 Size: 7933 BamHI G'GATC_C Cuts at: 5263 5630 5263 Size: 367 7566 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2830 5254 6709 618 Size: 325 217 627 1043 2424 1455 1842 Fragments arranged by size: 2424 1842 1455 1043 627 325 217 BanII G_rGCy'C Cuts at: 1391 2319 5252 1391 Size: 928 2933 4072 Fragments arranged by size: 4072 2933 928 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2441 3324 3588 928 Size: 971 542 883 264 5273 Fragments arranged by size: 5273 971 883 542 264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6124 6158 1903 Size: 34 4187 34 3678 Fragments arranged by size: 4187 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5825 7035 5825 Size: 1210 6723 BclI T'GATC_A Cuts at: 1548 1548 Size: 7933 BfrBI ATG'CAT Cuts at: 2366 3535 2366 Size: 1169 6764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6544 136 Size: 107 122 71 631 605 4872 1525 Fragments arranged by size: 4872 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5106 7928 5106 Size: 2822 5111 BlpI GC'TnA_GC Cuts at: 3474 5350 3474 Size: 1876 6057 Bme1580I G_kGCm'C Cuts at: 1790 2319 5994 7150 1790 Size: 529 3675 1156 2573 Fragments arranged by size: 3675 2573 1156 529 BmgBI CAC'GTC Cuts at: 1318 2682 1318 Size: 1364 6569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2159 2197 5664 Size: 416 53 83 399 24 742 38 3467 Cuts at: 5664 6622 442 Size: 958 1753 Fragments arranged by size: 3467 1753 958 742 416 399 83 53 38 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7933 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2517 2517 Size: 7933 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2130 3425 3526 6593 1239 Size: 891 1295 101 3067 2579 Fragments arranged by size: 3067 2579 1295 891 101 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2928 3183 6058 6923 7221 2928 Size: 255 2875 865 298 3640 Fragments arranged by size: 3640 2875 865 298 255 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2071 6596 882 Size: 617 572 4525 2219 Fragments arranged by size: 4525 2219 617 572 BsaAI yAC'GTr Cuts at: 493 1204 3795 5724 493 Size: 711 2591 1929 2702 Fragments arranged by size: 2702 2591 1929 711 BsaBI GATnn'nnATC Cuts at: 7927 7927 Size: 7933 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3184 5740 6122 6757 Size: 53 83 186 936 1651 2556 382 635 Cuts at: 6757 275 Size: 1451 Fragments arranged by size: 2556 1651 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6366 6764 7038 7185 7596 6366 Size: 398 274 147 411 6703 Fragments arranged by size: 6703 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7708 Fragments arranged by size: 7708 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 3348 1186 Size: 883 1279 5771 Fragments arranged by size: 5771 1279 883 BseYI C'CCAG_C Cuts at: 7136 7632 7136 Size: 496 7437 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2704 1163 Size: 573 968 6392 Fragments arranged by size: 6392 968 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5383 6144 7172 1540 Size: 275 186 3382 761 1028 2301 Fragments arranged by size: 3382 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3489 4615 5994 6079 7150 3489 Size: 1126 1379 85 1071 4272 Fragments arranged by size: 4272 1379 1126 1071 85 BsiWI C'GTAC_G Cuts at: 5125 5125 Size: 7933 BsmI GAATG_Cn' Cuts at: 5444 5537 5444 Size: 93 7840 BsmBI CGTCTCn'nnnn_ Cuts at: 5115 5115 Size: 7933 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2319 3489 4615 5252 5994 6079 7150 Size: 399 529 1170 1126 637 742 85 1071 Cuts at: 7150 1391 Size: 2174 Fragments arranged by size: 2174 1170 1126 1071 742 637 529 399 85 BspEI T'CCGG_A Cuts at: 7596 7596 Size: 7933 BspHI T'CATG_A Cuts at: 4585 5822 7552 4585 Size: 1237 1730 4966 Fragments arranged by size: 4966 1730 1237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5288 844 Size: 4444 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2457 3112 3448 5820 1394 Size: 429 634 655 336 2372 3507 Fragments arranged by size: 3507 2372 655 634 429 336 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 3123 3913 6428 6602 65 Size: 1183 889 986 790 2515 174 1396 Fragments arranged by size: 2515 1396 1183 986 889 790 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6577 6764 1700 Size: 110 4767 187 2869 Fragments arranged by size: 4767 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3959 96 Size: 3863 4070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7933 BssSI C'ACGA_G Cuts at: 5993 7005 5993 Size: 1012 6921 BstAPI GCAn_nnn'nTGC Cuts at: 794 3134 3470 4727 794 Size: 2340 336 1257 4000 Fragments arranged by size: 4000 2340 1257 336 BstBI TT'CG_AA Cuts at: 6737 6737 Size: 7933 BstEII G'GTnAC_C Cuts at: 1184 2414 1184 Size: 1230 6703 BstYI r'GATC_y Cuts at: 1972 2385 4255 5106 5263 5630 6015 6032 7484 Size: 413 1870 851 157 367 385 17 1452 Cuts at: 7484 7570 7582 7928 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1870 1452 851 413 385 367 346 157 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2826 2932 513 Size: 552 1761 106 5514 Fragments arranged by size: 5514 1761 552 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2322 487 Size: 683 56 1096 6098 Fragments arranged by size: 6098 1096 683 56 BtsI GCAGTG_nn' Cuts at: 5449 6243 6263 5449 Size: 794 20 7119 Fragments arranged by size: 7119 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7933 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2253 2288 529 Size: 35 1689 35 6174 Fragments arranged by size: 6174 1689 35 35 DraI TTT'AAA Cuts at: 3012 3709 5237 3012 Size: 697 1528 5708 Fragments arranged by size: 5708 1528 697 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6940 809 Size: 507 5624 1802 Fragments arranged by size: 5624 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5380 6269 7898 Size: 54 1425 50 275 18 3550 889 1629 Cuts at: 7898 8 Size: 43 Fragments arranged by size: 3550 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5380 1537 Size: 275 3568 4090 Fragments arranged by size: 4090 3568 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2424 4455 4758 5006 5863 1353 Size: 1071 2031 303 248 857 3423 Fragments arranged by size: 3423 2031 1071 857 303 248 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6520 6894 7040 387 Size: 1064 138 4931 374 146 1280 Fragments arranged by size: 4931 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 3425 3526 4007 4848 5996 6593 Size: 393 498 1295 101 481 841 1148 597 Cuts at: 6593 7380 7648 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1295 1148 841 787 597 498 481 393 268 101 EcoNI CCTnn'n_nnAGG Cuts at: 3090 3426 4863 3090 Size: 336 1437 6160 Fragments arranged by size: 6160 1437 336 EcoRI G'AATT_C Cuts at: 2721 5242 5620 2721 Size: 2521 378 5034 Fragments arranged by size: 5034 2521 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3387 4746 3387 Size: 1359 6574 FspI TGC'GCA Cuts at: 6439 6439 Size: 7933 HaeII r_GCGC'y Cuts at: 1678 7080 1678 Size: 5402 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2969 3001 3602 3634 4298 Size: 32 135 32 1397 32 601 32 664 Cuts at: 4298 4330 6574 6606 6648 6680 1373 Size: 32 2244 32 42 32 2626 Fragments arranged by size: 2626 2244 1397 664 601 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5277 5523 669 Size: 988 281 3339 246 3079 Fragments arranged by size: 3339 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2131 3273 4528 748 Size: 1383 1142 1255 4153 Fragments arranged by size: 4153 1383 1255 1142 HpaI GTT'AAC Cuts at: 5523 5523 Size: 7933 KpnI G_GTAC'C Cuts at: 5258 5258 Size: 7933 MfeI C'AATT_G Cuts at: 2583 5532 2583 Size: 2949 4984 MluI A'CGCG_T Cuts at: 7809 7809 Size: 7933 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3388 3521 4053 4283 5062 7047 7231 1305 Size: 2083 133 532 230 779 1985 184 2007 Fragments arranged by size: 2083 2007 1985 779 532 230 184 133 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6111 Fragments arranged by size: 6111 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2104 2245 4371 5227 5891 6250 6409 Size: 648 938 141 2126 856 664 359 159 Cuts at: 6409 518 Size: 2042 Fragments arranged by size: 2126 2042 938 856 664 648 359 159 141 MspA1I CmG'CkG Cuts at: 1606 2885 3071 5349 6026 7174 7419 1606 Size: 1279 186 2278 677 1148 245 2120 Fragments arranged by size: 2278 2120 1279 1148 677 245 186 NcoI C'CATG_G Cuts at: 513 1065 2826 2932 513 Size: 552 1761 106 5514 Fragments arranged by size: 5514 1761 552 106 NdeI CA'TA_TG Cuts at: 387 3399 3577 5176 387 Size: 3012 178 1599 3144 Fragments arranged by size: 3144 3012 1599 178 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7933 NotI GC'GGCC_GC Cuts at: 5380 5380 Size: 7933 NsiI A_TGCA'T Cuts at: 2368 3537 2368 Size: 1169 6764 NspI r_CATG'y Cuts at: 1132 1229 2594 2852 3176 3535 4111 4146 4323 Size: 97 1365 258 324 359 576 35 177 Cuts at: 4323 5291 6836 1132 Size: 968 1545 2229 Fragments arranged by size: 2229 1545 1365 968 576 359 324 258 177 97 35 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7933 PciI A'CATG_T Cuts at: 3172 4142 4319 6832 3172 Size: 970 177 2513 4273 Fragments arranged by size: 4273 2513 970 177 PflMI CCAn_nnn'nTGG Cuts at: 1108 2915 1108 Size: 1807 6126 PfoI T'CCnGG_A Cuts at: 3441 3441 Size: 7933 PmeI GTTT'AAAC Cuts at: 5237 5237 Size: 7933 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6002 6034 7515 7547 6002 Size: 32 1481 32 6388 Fragments arranged by size: 6388 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7933 PsiI TTA'TAA Cuts at: 5503 5503 Size: 7933 PspOMI G'GGCC_C Cuts at: 2315 2315 Size: 7933 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2776 2808 5043 5075 2776 Size: 32 2235 32 5634 Fragments arranged by size: 5634 2235 32 32 PstI C_TGCA'G Cuts at: 830 2884 5285 830 Size: 2054 2401 3478 Fragments arranged by size: 3478 2401 2054 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7933 PvuII CAG'CTG Cuts at: 2885 3071 2885 Size: 186 7747 SalI G'TCGA_C Cuts at: 1936 5275 1936 Size: 3339 4594 SbfI CC_TGCA'GG Cuts at: 5285 5285 Size: 7933 ScaI AGT'ACT Cuts at: 6181 7893 6181 Size: 1712 6221 SfcI C'TryA_G Cuts at: 826 1045 2302 2530 2558 2880 4774 5281 6416 Size: 219 1257 228 28 322 1894 507 1135 Cuts at: 6416 7097 7288 826 Size: 681 191 1471 Fragments arranged by size: 1894 1471 1257 1135 681 507 322 228 219 191 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7933 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7933 SmaI CCC'GGG Cuts at: 1872 5260 1872 Size: 3388 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2943 3198 6037 6938 7200 820 Size: 197 926 1000 255 2839 901 262 1553 Fragments arranged by size: 2839 1553 1000 926 901 262 255 197 SnaBI TAC'GTA Cuts at: 493 3795 5724 493 Size: 3302 1929 2702 Fragments arranged by size: 3302 2702 1929 SpeI A'CTAG_T Cuts at: 152 152 Size: 7933 SphI G_CATG'C Cuts at: 1132 5291 1132 Size: 4159 3774 SspI AAT'ATT Cuts at: 5 52 2036 2524 2701 2953 4088 5857 5 Size: 47 1984 488 177 252 1135 1769 2081 Fragments arranged by size: 2081 1984 1769 1135 488 252 177 47 StuI AGG'CCT Cuts at: 1517 4135 1517 Size: 2618 5315 StyI C'CwwG_G Cuts at: 513 1065 2826 2932 4401 4639 4834 5372 513 Size: 552 1761 106 1469 238 195 538 3074 Fragments arranged by size: 3074 1761 1469 552 538 238 195 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2031 2433 4802 5960 5977 6130 6315 Size: 1255 451 402 2369 1158 17 153 185 Cuts at: 6315 6760 325 Size: 445 1498 Fragments arranged by size: 2369 1498 1255 1158 451 445 402 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3959 6179 7891 Size: 274 80 33 51 1443 1982 2220 1712 Cuts at: 7891 96 Size: 138 Fragments arranged by size: 2220 1982 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7893 7925 7893 Size: 32 7901 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7933 XbaI T'CTAG_A Cuts at: 4538 5141 5269 4538 Size: 603 128 7202 Fragments arranged by size: 7202 603 128 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7933 XmaI C'CCGG_G Cuts at: 1870 5258 1870 Size: 3388 4545 XmnI GAAnn'nnTTC Cuts at: 2133 4529 4984 6062 2133 Size: 2396 455 1078 4004 Fragments arranged by size: 4004 2396 1078 455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3185 5741 276 Size: 53 83 186 936 1651 2556 2468 Fragments arranged by size: 2556 2468 1651 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI