(Circular) (Six-base) MAPSORT of: pFN21AA1296.seq Check: 8207 from: 1 to: 7048 pFN21AA1296 7048 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 4858 278 Size: 53 83 186 936 1909 1413 2468 Fragments arranged by size: 2468 1909 1413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4391 1937 Size: 2454 4594 Acc65I G'GTAC_C Cuts at: 4369 4369 Size: 7048 AclI AA'CG_TT Cuts at: 1563 5175 5548 1563 Size: 3612 373 3063 Fragments arranged by size: 3612 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2310 2919 3038 3168 3308 3692 3892 5111 Size: 678 609 119 130 140 384 200 1219 Cuts at: 5111 6495 6763 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1219 678 609 384 268 200 140 130 119 AfeI AGC'GCT Cuts at: 3572 3572 Size: 7048 AflII C'TTAA_G Cuts at: 820 1017 2752 820 Size: 197 1735 5116 Fragments arranged by size: 5116 1735 197 AflIII A'CryG_T Cuts at: 1203 5947 6924 1203 Size: 4744 977 1327 Fragments arranged by size: 4744 1327 977 AgeI A'CCGG_T Cuts at: 5879 5879 Size: 7048 AhdI GACnn_n'nnGTC Cuts at: 5777 5777 Size: 7048 AleI CACnn'nnGTG Cuts at: 1166 4010 1166 Size: 2844 4204 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7048 AlwNI CAG_nnn'CTG Cuts at: 2624 3331 3670 4791 6363 2624 Size: 707 339 1121 1572 3309 Fragments arranged by size: 3309 1572 1121 707 339 ApaLI G'TGCA_C Cuts at: 5105 6261 5105 Size: 1156 5892 ApoI r'AATT_y Cuts at: 1493 3177 3285 3428 3647 4357 4583 4735 6844 Size: 1684 108 143 219 710 226 152 2109 Cuts at: 6844 1493 Size: 1697 Fragments arranged by size: 2109 1697 1684 710 226 219 152 143 108 AseI AT'TA_AT Cuts at: 160 1032 5602 160 Size: 872 4570 1606 Fragments arranged by size: 4570 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 AvaI C'yCGr_G Cuts at: 1870 1943 3973 4373 1870 Size: 73 2030 400 4545 Fragments arranged by size: 4545 2030 400 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3879 3912 4325 4358 3879 Size: 33 413 33 6569 Fragments arranged by size: 6569 413 33 33 BamHI G'GATC_C Cuts at: 2182 3969 4378 4745 2182 Size: 1787 409 367 4485 Fragments arranged by size: 4485 1787 409 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 4369 5824 618 Size: 325 217 627 266 2316 1455 1842 Fragments arranged by size: 2316 1842 1455 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 2273 3093 3155 3250 3342 4367 1391 Size: 882 820 62 95 92 1025 4072 Fragments arranged by size: 4072 1025 882 820 95 92 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2144 2226 2907 3117 3445 928 Size: 971 245 82 681 210 328 4531 Fragments arranged by size: 4531 971 681 328 245 210 82 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2567 2601 3766 3800 5239 5273 1903 Size: 34 630 34 1165 34 1439 34 3678 Fragments arranged by size: 3678 1439 1165 630 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4940 6150 4940 Size: 1210 5838 BclI T'GATC_A Cuts at: 1548 3724 4020 1548 Size: 2176 296 4576 Fragments arranged by size: 4576 2176 296 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3531 5659 136 Size: 107 122 71 631 605 1859 2128 1525 Fragments arranged by size: 2128 1859 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2869 7043 2869 Size: 4174 2874 BlpI GC'TnA_GC Cuts at: 3154 4465 3154 Size: 1311 5737 Bme1580I G_kGCm'C Cuts at: 1790 5109 6265 1790 Size: 3319 1156 2573 Fragments arranged by size: 3319 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7048 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2211 3052 4779 Size: 416 53 83 399 24 794 841 1727 Cuts at: 4779 5737 442 Size: 958 1753 Fragments arranged by size: 1753 1727 958 841 794 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7048 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 7048 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2330 5708 1239 Size: 1091 3378 2579 Fragments arranged by size: 3378 2579 1091 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3326 4101 5173 6038 6336 3326 Size: 775 1072 865 298 4038 Fragments arranged by size: 4038 1072 865 775 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3625 5711 882 Size: 617 2126 2086 2219 Fragments arranged by size: 2219 2126 2086 617 BsaAI yAC'GTr Cuts at: 493 1204 4012 4839 493 Size: 711 2808 827 2702 Fragments arranged by size: 2808 2702 827 711 BsaBI GATnn'nnATC Cuts at: 2868 7042 2868 Size: 4174 2874 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3442 3523 3534 4855 Size: 53 83 186 936 1909 81 11 1321 Cuts at: 4855 5237 5872 275 Size: 382 635 1451 Fragments arranged by size: 1909 1451 1321 936 635 382 186 83 81 53 11 BsaWI w'CCGG_w Cuts at: 2790 3172 5481 5879 6153 6300 6711 2790 Size: 382 2309 398 274 147 411 3127 Fragments arranged by size: 3127 2309 411 398 382 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2896 2926 2979 3009 3687 Size: 30 165 30 1493 30 53 30 678 Cuts at: 3687 3717 4227 4257 1178 Size: 30 510 30 3969 Fragments arranged by size: 3969 1493 678 510 165 53 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3575 4063 4066 1186 Size: 2389 488 3 4168 Fragments arranged by size: 4168 2389 488 3 BseYI C'CCAG_C Cuts at: 2376 2998 4122 6251 6747 2376 Size: 622 1124 2129 496 2677 Fragments arranged by size: 2677 2129 1124 622 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2182 3105 3857 1163 Size: 573 446 923 752 4354 Fragments arranged by size: 4354 923 752 573 446 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3757 4498 5259 6287 1540 Size: 275 186 1756 741 761 1028 2301 Fragments arranged by size: 2301 1756 1028 761 741 275 186 BsiHKAI G_wGCw'C Cuts at: 2574 2744 3155 5109 5194 6265 2574 Size: 170 411 1954 85 1071 3357 Fragments arranged by size: 3357 1954 1071 411 170 85 BsmI GAATG_Cn' Cuts at: 4559 4652 4559 Size: 93 6955 BsmBI CGTCTCn'nnnn_ Cuts at: 3472 3472 Size: 7048 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2273 2574 2744 3093 3155 3250 3342 Size: 399 483 301 170 349 62 95 92 Cuts at: 3342 4367 5109 5194 6265 1391 Size: 1025 742 85 1071 2174 Fragments arranged by size: 2174 1071 1025 742 483 399 349 301 170 95 92 85 62 BspEI T'CCGG_A Cuts at: 2790 3172 6711 2790 Size: 382 3539 3127 Fragments arranged by size: 3539 3127 382 BspHI T'CATG_A Cuts at: 3445 4937 6667 3445 Size: 1492 1730 3826 Fragments arranged by size: 3826 1730 1492 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3643 3829 3931 4071 4403 844 Size: 2799 186 102 140 332 3489 Fragments arranged by size: 3489 2799 332 186 140 102 BsrBI CCG'CTC Cuts at: 1394 1823 2213 3006 4935 1394 Size: 429 390 793 1929 3507 Fragments arranged by size: 3507 1929 793 429 390 BsrDI GCAATG_nn' Cuts at: 65 1248 2071 5543 5717 65 Size: 1183 823 3472 174 1396 Fragments arranged by size: 3472 1396 1183 823 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5692 5879 1700 Size: 110 3882 187 2869 Fragments arranged by size: 3882 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7048 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7048 BssSI C'ACGA_G Cuts at: 5108 6120 5108 Size: 1012 6036 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7048 BstBI TT'CG_AA Cuts at: 3436 5852 3436 Size: 2416 4632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7048 BstYI r'GATC_y Cuts at: 1972 2182 2620 2838 2869 3256 3969 4378 4745 Size: 210 438 218 31 387 713 409 367 Cuts at: 4745 5130 5147 6599 6685 6697 7043 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 713 438 409 387 385 367 346 218 210 86 31 17 12 Bsu36I CC'TnA_GG Cuts at: 2155 2274 2155 Size: 119 6929 BtgI C'CryG_G Cuts at: 513 1065 2766 3420 3751 513 Size: 552 1701 654 331 3810 Fragments arranged by size: 3810 1701 654 552 331 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2772 4245 487 Size: 683 56 1546 1473 3290 Fragments arranged by size: 3290 1546 1473 683 56 BtsI GCAGTG_nn' Cuts at: 3231 3929 4095 4096 4564 5358 5378 3231 Size: 698 166 1 468 794 20 4901 Fragments arranged by size: 4901 794 698 468 166 20 1 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7048 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2533 2568 529 Size: 35 1969 35 5009 Fragments arranged by size: 5009 1969 35 35 DraI TTT'AAA Cuts at: 3658 4352 3658 Size: 694 6354 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6055 809 Size: 507 4739 1802 Fragments arranged by size: 4739 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2982 3754 4495 Size: 54 1425 50 275 18 1152 772 741 Cuts at: 4495 5384 7013 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1152 889 772 741 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3754 4495 1537 Size: 275 1942 741 4090 Fragments arranged by size: 4090 1942 741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2389 2632 2732 3013 3224 3327 3495 4126 Size: 1036 243 100 281 211 103 168 631 Cuts at: 4126 4978 1353 Size: 852 3423 Fragments arranged by size: 3423 1036 852 631 281 243 211 168 103 100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2521 5635 6009 6155 387 Size: 1064 138 932 3114 374 146 1280 Fragments arranged by size: 3114 1280 1064 932 374 146 138 EcoICRI GAG'CTC Cuts at: 3153 3153 Size: 7048 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2330 2919 3038 3168 3308 3692 Size: 393 678 20 589 119 130 140 384 Cuts at: 3692 3892 5111 5708 6495 6763 1239 Size: 200 1219 597 787 268 1524 Fragments arranged by size: 1524 1219 787 678 597 589 393 384 268 200 140 130 119 20 EcoO109I rG'GnC_Cy Cuts at: 3328 3580 3328 Size: 252 6796 EcoRI G'AATT_C Cuts at: 4357 4735 4357 Size: 378 6670 EcoRV GAT'ATC Cuts at: 4240 4240 Size: 7048 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3479 3892 3479 Size: 413 6635 FspI TGC'GCA Cuts at: 5554 5554 Size: 7048 HaeII r_GCGC'y Cuts at: 1678 3574 6195 1678 Size: 1896 2621 2531 Fragments arranged by size: 2621 2531 1896 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2542 2574 2612 2644 2861 Size: 32 135 32 970 32 38 32 217 Cuts at: 2861 2893 2894 2926 3365 3397 3535 3567 3978 Size: 32 1 32 439 32 138 32 411 Cuts at: 3978 4010 5689 5721 5763 5795 1373 Size: 32 1679 32 42 32 2626 Fragments arranged by size: 2626 1679 970 439 411 217 138 135 42 38 32 32 32 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 3828 4392 4638 669 Size: 988 281 1890 564 246 3079 Fragments arranged by size: 3079 1890 988 564 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7048 HpaI GTT'AAC Cuts at: 4638 4638 Size: 7048 KpnI G_GTAC'C Cuts at: 4373 4373 Size: 7048 MfeI C'AATT_G Cuts at: 4647 4647 Size: 7048 MluI A'CGCG_T Cuts at: 6924 6924 Size: 7048 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3237 3566 4200 6162 6346 1305 Size: 1932 329 634 1962 184 2007 Fragments arranged by size: 2007 1962 1932 634 329 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5226 Fragments arranged by size: 5226 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2132 4010 5006 5365 5524 518 Size: 648 966 1878 996 359 159 2042 Fragments arranged by size: 2042 1878 996 966 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3404 4464 5141 6289 6534 1606 Size: 1798 1060 677 1148 245 2120 Fragments arranged by size: 2120 1798 1148 1060 677 245 NcoI C'CATG_G Cuts at: 513 1065 2766 513 Size: 552 1701 4795 Fragments arranged by size: 4795 1701 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7048 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7048 NotI GC'GGCC_GC Cuts at: 4495 4495 Size: 7048 NspI r_CATG'y Cuts at: 1132 1229 4406 5951 1132 Size: 97 3177 1545 2229 Fragments arranged by size: 3177 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7048 PciI A'CATG_T Cuts at: 5947 5947 Size: 7048 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7048 PfoI T'CCnGG_A Cuts at: 3193 3193 Size: 7048 PmeI GTTT'AAAC Cuts at: 4352 4352 Size: 7048 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5117 5149 6630 6662 5117 Size: 32 1481 32 5503 Fragments arranged by size: 5503 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3580 3580 Size: 7048 PshAI GACnn'nnGTC Cuts at: 1613 4149 1613 Size: 2536 4512 PsiI TTA'TAA Cuts at: 3291 3714 4618 3291 Size: 423 904 5721 Fragments arranged by size: 5721 904 423 PstI C_TGCA'G Cuts at: 830 3690 4400 830 Size: 2860 710 3478 Fragments arranged by size: 3478 2860 710 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7048 PvuII CAG'CTG Cuts at: 3404 3404 Size: 7048 SacI G_AGCT'C Cuts at: 3155 3155 Size: 7048 SalI G'TCGA_C Cuts at: 1936 4390 1936 Size: 2454 4594 SapI GCTCTTCn'nnn_ Cuts at: 2732 3013 2732 Size: 281 6767 SbfI CC_TGCA'GG Cuts at: 4400 4400 Size: 7048 ScaI AGT'ACT Cuts at: 5296 7008 5296 Size: 1712 5336 SexAI A'CCwGG_T Cuts at: 2302 2302 Size: 7048 SfcI C'TryA_G Cuts at: 826 1045 2172 2239 2404 3686 4396 5531 6212 Size: 219 1127 67 165 1282 710 1135 681 Cuts at: 6212 6403 826 Size: 191 1471 Fragments arranged by size: 1471 1282 1135 1127 710 681 219 191 165 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7048 SmaI CCC'GGG Cuts at: 1872 3975 4375 1872 Size: 2103 400 4545 Fragments arranged by size: 4545 2103 400 SmlI C'TyrA_G Cuts at: 820 1017 1943 2752 3341 4116 5152 6053 6315 Size: 197 926 809 589 775 1036 901 262 Cuts at: 6315 820 Size: 1553 Fragments arranged by size: 1553 1036 926 901 809 775 589 262 197 SnaBI TAC'GTA Cuts at: 493 4839 493 Size: 4346 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7048 SphI G_CATG'C Cuts at: 1132 4406 1132 Size: 3274 3774 SspI AAT'ATT Cuts at: 5 52 4972 5 Size: 47 4920 2081 Fragments arranged by size: 4920 2081 47 StuI AGG'CCT Cuts at: 1517 2160 1517 Size: 643 6405 StyI C'CwwG_G Cuts at: 513 1065 2397 2766 4487 513 Size: 552 1332 369 1721 3074 Fragments arranged by size: 3074 1721 1332 552 369 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5075 5092 5245 5430 5875 325 Size: 1255 3495 17 153 185 445 1498 Fragments arranged by size: 3495 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2874 5294 7006 Size: 274 80 33 51 1443 897 2420 1712 Cuts at: 7006 96 Size: 138 Fragments arranged by size: 2420 1712 1443 897 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2896 2928 2979 3011 7008 7040 2896 Size: 32 51 32 3997 32 2904 Fragments arranged by size: 3997 2904 51 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7048 XbaI T'CTAG_A Cuts at: 4384 4384 Size: 7048 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2773 4009 2773 Size: 1236 5812 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7048 XmaI C'CCGG_G Cuts at: 1870 3973 4373 1870 Size: 2103 400 4545 Fragments arranged by size: 4545 2103 400 XmnI GAAnn'nnTTC Cuts at: 2494 3432 5177 2494 Size: 938 1745 4365 Fragments arranged by size: 4365 1745 938 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 4856 276 Size: 53 83 186 936 1909 1413 2468 Fragments arranged by size: 2468 1909 1413 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI