Restriction map for
pFN21AA1296
(Linear) (Six-base) MAPSORT of: KIAA1296.seq Check: 3029 from: 1 to: 2343
KIAA1296.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AatII G_ACGT'C
Cuts at: 0 1440 2343
Size: 1440 903
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 914 1033 1163 1303 1687 1887 2343
Size: 305 609 119 130 140 384 200 456
Fragments arranged by size:
609 456 384 305 200 140 130 119
AfeI AGC'GCT
Cuts at: 0 1567 2343
Size: 1567 776
AflII C'TTAA_G
Cuts at: 0 747 2343
Size: 747 1596
AleI CACnn'nnGTG
Cuts at: 0 2005 2343
Size: 2005 338
AlwNI CAG_nnn'CTG
Cuts at: 0 619 1326 1665 2343
Size: 619 707 339 678
Fragments arranged by size:
707 678 619 339
ApoI r'AATT_y
Cuts at: 0 1172 1280 1423 1642 2343
Size: 1172 108 143 219 701
Fragments arranged by size:
1172 701 219 143 108
AvaI C'yCGr_G
Cuts at: 0 1968 2343
Size: 1968 375
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1874 1907 2320 2343
Size: 1874 33 413 23
Fragments arranged by size:
1874 413 33 23
BamHI G'GATC_C
Cuts at: 0 177 1964 2343
Size: 177 1787 379
Fragments arranged by size:
1787 379 177
BanI G'GyrC_C
Cuts at: 0 48 2343
Size: 48 2295
BanII G_rGCy'C
Cuts at: 0 268 1088 1150 1245 1337 2343
Size: 268 820 62 95 92 1006
Fragments arranged by size:
1006 820 268 95 92 62
BbsI GAAGACnn'nnnn_
Cuts at: 0 139 221 902 1112 1440 2343
Size: 139 82 681 210 328 903
Fragments arranged by size:
903 681 328 210 139 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 562 596 1761 1795 2343
Size: 562 34 1165 34 548
Fragments arranged by size:
1165 562 548 34 34
BclI T'GATC_A
Cuts at: 0 1719 2015 2343
Size: 1719 296 328
Fragments arranged by size:
1719 328 296
BglI GCCn_nnn'nGGC
Cuts at: 0 1526 2343
Size: 1526 817
BglII A'GATC_T
Cuts at: 0 864 2343
Size: 864 1479
BlpI GC'TnA_GC
Cuts at: 0 1149 2343
Size: 1149 1194
BmrI ACTGGGnnnn_n'
Cuts at: 0 206 1047 2343
Size: 206 841 1296
Fragments arranged by size:
1296 841 206
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 666 2343
Size: 666 1677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 2343
Size: 325 2018
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1321 2096 2343
Size: 1321 775 247
Fragments arranged by size:
1321 775 247
BsaI GGTCTCn'nnnn_
Cuts at: 0 1620 2343
Size: 1620 723
BsaAI yAC'GTr
Cuts at: 0 2007 2343
Size: 2007 336
BsaBI GATnn'nnATC
Cuts at: 0 863 2343
Size: 863 1480
BsaHI Gr'CG_yC
Cuts at: 0 1437 1518 1529 2343
Size: 1437 81 11 814
Fragments arranged by size:
1437 814 81 11
BsaWI w'CCGG_w
Cuts at: 0 785 1167 2343
Size: 785 382 1176
Fragments arranged by size:
1176 785 382
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 891 921 974 1004 1682 1712 2222 2252
Size: 891 30 53 30 678 30 510 30
Cuts at: 2252 2343
Size: 91
Fragments arranged by size:
891 678 510 91 53 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1570 2058 2061 2343
Size: 1570 488 3 282
Fragments arranged by size:
1570 488 282 3
BseYI C'CCAG_C
Cuts at: 0 371 993 2117 2343
Size: 371 622 1124 226
Fragments arranged by size:
1124 622 371 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 1100 1852 2343
Size: 177 923 752 491
Fragments arranged by size:
923 752 491 177
BsiEI CG_ry'CG
Cuts at: 0 1752 2343
Size: 1752 591
BsiHKAI G_wGCw'C
Cuts at: 0 569 739 1150 2343
Size: 569 170 411 1193
Fragments arranged by size:
1193 569 411 170
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1467 2343
Size: 1467 876
Bsp1286I G_dGCh'C
Cuts at: 0 268 569 739 1088 1150 1245 1337 2343
Size: 268 301 170 349 62 95 92 1006
Fragments arranged by size:
1006 349 301 268 170 95 92 62
BspEI T'CCGG_A
Cuts at: 0 785 1167 2343
Size: 785 382 1176
Fragments arranged by size:
1176 785 382
BspHI T'CATG_A
Cuts at: 0 1440 2343
Size: 1440 903
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1638 1824 1926 2066 2343
Size: 1638 186 102 140 277
Fragments arranged by size:
1638 277 186 140 102
BsrBI CCG'CTC
Cuts at: 0 208 1001 2343
Size: 208 793 1342
Fragments arranged by size:
1342 793 208
BsrDI GCAATG_nn'
Cuts at: 0 66 2343
Size: 66 2277
BstBI TT'CG_AA
Cuts at: 0 1431 2343
Size: 1431 912
BstYI r'GATC_y
Cuts at: 0 177 615 833 864 1251 1964 2343
Size: 177 438 218 31 387 713 379
Fragments arranged by size:
713 438 387 379 218 177 31
Bsu36I CC'TnA_GG
Cuts at: 0 150 269 2343
Size: 150 119 2074
Fragments arranged by size:
2074 150 119
BtgI C'CryG_G
Cuts at: 0 761 1415 1746 2343
Size: 761 654 331 597
Fragments arranged by size:
761 654 597 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 767 2240 2343
Size: 767 1473 103
Fragments arranged by size:
1473 767 103
BtsI GCAGTG_nn'
Cuts at: 0 1226 1924 2090 2091 2343
Size: 1226 698 166 1 252
Fragments arranged by size:
1226 698 252 166 1
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 528 563 2343
Size: 528 35 1780
Fragments arranged by size:
1780 528 35
DraI TTT'AAA
Cuts at: 0 1653 2343
Size: 1653 690
EaeI y'GGCC_r
Cuts at: 0 977 1749 2343
Size: 977 772 594
Fragments arranged by size:
977 772 594
EagI C'GGCC_G
Cuts at: 0 1749 2343
Size: 1749 594
EarI CTCTTCn'nnn_
Cuts at: 0 384 627 727 1008 1219 1322 1490 2121
Size: 384 243 100 281 211 103 168 631
Cuts at: 2121 2343
Size: 222
Fragments arranged by size:
631 384 281 243 222 211 168 103
100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 516 2343
Size: 516 1827
EcoICRI GAG'CTC
Cuts at: 0 1148 2343
Size: 1148 1195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 325 914 1033 1163 1303 1687 1887
Size: 305 20 589 119 130 140 384 200
Cuts at: 1887 2343
Size: 456
Fragments arranged by size:
589 456 384 305 200 140 130 119
20
EcoO109I rG'GnC_Cy
Cuts at: 0 1323 1575 2343
Size: 1323 252 768
Fragments arranged by size:
1323 768 252
EcoRV GAT'ATC
Cuts at: 0 2235 2343
Size: 2235 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1474 1887 2343
Size: 1474 413 456
Fragments arranged by size:
1474 456 413
HaeII r_GCGC'y
Cuts at: 0 1569 2343
Size: 1569 774
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 537 569 607 639 856 888 889 921
Size: 537 32 38 32 217 32 1 32
Cuts at: 921 1360 1392 1530 1562 1973 2005 2343
Size: 439 32 138 32 411 32 338
Fragments arranged by size:
537 439 411 338 217 138 38 32
32 32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 0 1823 2343
Size: 1823 520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1232 1561 2195 2343
Size: 1232 329 634 148
Fragments arranged by size:
1232 634 329 148
MslI CAynn'nnrTG
Cuts at: 0 127 2005 2343
Size: 127 1878 338
Fragments arranged by size:
1878 338 127
MspA1I CmG'CkG
Cuts at: 0 1399 2343
Size: 1399 944
NcoI C'CATG_G
Cuts at: 0 761 2343
Size: 761 1582
PfoI T'CCnGG_A
Cuts at: 0 1188 2343
Size: 1188 1155
PpuMI rG'GwC_Cy
Cuts at: 0 1575 2343
Size: 1575 768
PshAI GACnn'nnGTC
Cuts at: 0 2144 2343
Size: 2144 199
PsiI TTA'TAA
Cuts at: 0 1286 1709 2343
Size: 1286 423 634
Fragments arranged by size:
1286 634 423
PstI C_TGCA'G
Cuts at: 0 1685 2343
Size: 1685 658
PvuII CAG'CTG
Cuts at: 0 1399 2343
Size: 1399 944
SacI G_AGCT'C
Cuts at: 0 1150 2343
Size: 1150 1193
SapI GCTCTTCn'nnn_
Cuts at: 0 727 1008 2343
Size: 727 281 1335
Fragments arranged by size:
1335 727 281
SexAI A'CCwGG_T
Cuts at: 0 297 2343
Size: 297 2046
SfcI C'TryA_G
Cuts at: 0 167 234 399 1681 2343
Size: 167 67 165 1282 662
Fragments arranged by size:
1282 662 167 165 67
SmaI CCC'GGG
Cuts at: 0 1970 2343
Size: 1970 373
SmlI C'TyrA_G
Cuts at: 0 747 1336 2111 2343
Size: 747 589 775 232
Fragments arranged by size:
775 747 589 232
StuI AGG'CCT
Cuts at: 0 155 2343
Size: 155 2188
StyI C'CwwG_G
Cuts at: 0 392 761 2343
Size: 392 369 1582
Fragments arranged by size:
1582 392 369
TatI w'GTAC_w
Cuts at: 0 869 2343
Size: 869 1474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 891 923 974 1006 2343
Size: 891 32 51 32 1337
Fragments arranged by size:
1337 891 51 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 768 2004 2343
Size: 768 1236 339
Fragments arranged by size:
1236 768 339
XmaI C'CCGG_G
Cuts at: 0 1968 2343
Size: 1968 375
XmnI GAAnn'nnTTC
Cuts at: 0 489 1427 2343
Size: 489 938 916
Fragments arranged by size:
938 916 489
ZraI GAC'GTC
Cuts at: 0 1438 2343
Size: 1438 905
Enzymes that do cut:
AatII AcuI AfeI AflII AleI AlwNI ApoI AvaI
BaeI BamHI BanI BanII BbsI BcgI BclI BglI
BglII BlpI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I HincII MmeI MslI MspA1I NcoI
PfoI PpuMI PshAI PsiI PstI PvuII SacI SapI
SexAI SfcI SmaI SmlI StuI StyI TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BbeI
BbvCI BciVI BfrBI Bme1580I BmgBI BmtI Bpu10I BsiWI
BsmI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I ClaI DraIII DrdI EcoNI EcoRI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI