(Circular) (Six-base) MAPSORT of: pFN21AA1271.seq Check: 7148 from: 1 to: 6325 pFN21AA1271 6325 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2147 2377 2618 2881 3469 2147 Size: 230 241 263 588 5003 Fragments arranged by size: 5003 588 263 241 230 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2277 3288 3668 1937 Size: 340 1011 380 4594 Fragments arranged by size: 4594 1011 380 340 Acc65I G'GTAC_C Cuts at: 3646 3646 Size: 6325 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2037 2174 4388 5772 6040 1632 Size: 405 137 2214 1384 268 1917 Fragments arranged by size: 2214 1917 1384 405 268 137 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6128 AflIII A'CryG_T Cuts at: 1203 5224 6201 1203 Size: 4021 977 1327 Fragments arranged by size: 4021 1327 977 AgeI A'CCGG_T Cuts at: 5156 5156 Size: 6325 AhdI GACnn_n'nnGTC Cuts at: 5054 5054 Size: 6325 AleI CACnn'nnGTG Cuts at: 1166 3116 1166 Size: 1950 4375 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6325 AlwNI CAG_nnn'CTG Cuts at: 2692 2801 2957 3439 4068 5640 2692 Size: 109 156 482 629 1572 3377 Fragments arranged by size: 3377 1572 629 482 156 109 ApaI G_GGCC'C Cuts at: 2361 3357 2361 Size: 996 5329 ApaLI G'TGCA_C Cuts at: 4382 5538 4382 Size: 1156 5169 ApoI r'AATT_y Cuts at: 1493 2470 3634 3860 4012 6121 1493 Size: 977 1164 226 152 2109 1697 Fragments arranged by size: 2109 1697 1164 977 226 152 AseI AT'TA_AT Cuts at: 160 1032 4879 160 Size: 872 3847 1606 Fragments arranged by size: 3847 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 3301 3650 1870 Size: 73 1358 349 4545 Fragments arranged by size: 4545 1358 349 73 BamHI G'GATC_C Cuts at: 3405 3655 4022 3405 Size: 250 367 5708 Fragments arranged by size: 5708 367 250 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2449 2756 2869 3079 3191 Size: 325 217 627 662 307 113 210 112 Cuts at: 3191 3395 3520 3646 5101 618 Size: 204 125 126 1455 1842 Fragments arranged by size: 1842 1455 662 627 325 307 217 210 204 126 125 113 112 BanII G_rGCy'C Cuts at: 1391 2361 3357 3550 3644 1391 Size: 970 996 193 94 4072 Fragments arranged by size: 4072 996 970 193 94 BbeI G_GCGC'C Cuts at: 2453 3195 2453 Size: 742 5583 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2025 928 Size: 971 126 5228 Fragments arranged by size: 5228 971 126 BbvCI CC'TCA_GC Cuts at: 2564 2564 Size: 6325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3302 3336 4516 4550 1903 Size: 34 50 34 1281 34 1180 34 3678 Fragments arranged by size: 3678 1281 1180 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4217 5427 4217 Size: 1210 5115 BclI T'GATC_A Cuts at: 1548 1548 Size: 6325 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4936 136 Size: 107 122 71 631 605 3264 1525 Fragments arranged by size: 3264 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6320 6320 Size: 6325 BlpI GC'TnA_GC Cuts at: 2495 3208 3742 2495 Size: 713 534 5078 Fragments arranged by size: 5078 713 534 Bme1580I G_kGCm'C Cuts at: 1790 2361 2973 3357 3398 4386 5542 1790 Size: 571 612 384 41 988 1156 2573 Fragments arranged by size: 2573 1156 988 612 571 384 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6325 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2618 4056 5014 Size: 416 53 83 399 24 1201 1438 958 Cuts at: 5014 442 Size: 1753 Fragments arranged by size: 1753 1438 1201 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6325 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2563 2563 Size: 6325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2558 2563 2623 2839 3219 3461 3542 Size: 1106 213 5 60 216 380 242 81 Cuts at: 3542 4985 1239 Size: 1443 2579 Fragments arranged by size: 2579 1443 1106 380 242 216 213 81 60 5 Bpu10I CC'TnA_GC Cuts at: 2564 2564 Size: 6325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4450 5315 5613 4450 Size: 865 298 5162 Fragments arranged by size: 5162 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2109 2435 3158 4988 882 Size: 617 610 326 723 1830 2219 Fragments arranged by size: 2219 1830 723 617 610 326 BsaAI yAC'GTr Cuts at: 493 1204 3412 4116 493 Size: 711 2208 704 2702 Fragments arranged by size: 2702 2208 711 704 BsaBI GATnn'nnATC Cuts at: 6319 6319 Size: 6325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2450 3192 3616 4132 Size: 53 83 186 936 917 742 424 516 Cuts at: 4132 4514 5149 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 917 742 635 516 424 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3498 4758 5156 5430 5577 5988 3498 Size: 1260 398 274 147 411 3835 Fragments arranged by size: 3835 1260 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2318 2348 3365 3395 1178 Size: 30 165 30 915 30 1017 30 4108 Fragments arranged by size: 4108 1017 915 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2536 2758 2761 3176 3386 1186 Size: 1350 222 3 415 210 4125 Fragments arranged by size: 4125 1350 415 222 210 3 BseYI C'CCAG_C Cuts at: 2205 2950 3169 3425 5528 6024 2205 Size: 745 219 256 2103 496 2506 Fragments arranged by size: 2506 2103 745 496 256 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2125 2841 3027 3605 1163 Size: 573 389 716 186 578 3883 Fragments arranged by size: 3883 716 578 573 389 186 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 3775 4536 5564 1540 Size: 275 186 1498 276 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 276 275 186 BsiHKAI G_wGCw'C Cuts at: 3111 4386 4471 5542 3111 Size: 1275 85 1071 3894 Fragments arranged by size: 3894 1275 1071 85 BsmI GAATG_Cn' Cuts at: 3836 3929 3836 Size: 93 6232 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2361 2973 3111 3357 3398 3550 3644 Size: 399 571 612 138 246 41 152 94 Cuts at: 3644 4386 4471 5542 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 612 571 399 246 152 138 94 85 41 BspEI T'CCGG_A Cuts at: 5988 5988 Size: 6325 BspHI T'CATG_A Cuts at: 4214 5944 4214 Size: 1730 4595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2103 2147 2377 2618 2881 3469 3680 844 Size: 1259 44 230 241 263 588 211 3489 Fragments arranged by size: 3489 1259 588 263 241 230 211 44 BsrBI CCG'CTC Cuts at: 1394 1823 4212 1394 Size: 429 2389 3507 Fragments arranged by size: 3507 2389 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2071 2220 4820 4994 65 Size: 1183 823 149 2600 174 1396 Fragments arranged by size: 2600 1396 1183 823 174 149 BsrFI r'CCGG_y Cuts at: 1700 1810 3188 3609 4969 5156 1700 Size: 110 1378 421 1360 187 2869 Fragments arranged by size: 2869 1378 1360 421 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6325 BssSI C'ACGA_G Cuts at: 4385 5397 4385 Size: 1012 5313 BstAPI GCAn_nnn'nTGC Cuts at: 794 2061 2485 2801 794 Size: 1267 424 316 4318 Fragments arranged by size: 4318 1267 424 316 BstBI TT'CG_AA Cuts at: 5129 5129 Size: 6325 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6325 BstYI r'GATC_y Cuts at: 1972 3316 3405 3655 4022 4407 4424 5876 5962 Size: 1344 89 250 367 385 17 1452 86 Cuts at: 5962 5974 6320 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1344 385 367 346 250 89 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2656 3361 513 Size: 552 1591 705 3477 Fragments arranged by size: 3477 1591 705 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5586 Fragments arranged by size: 5586 683 56 BtsI GCAGTG_nn' Cuts at: 3015 3841 4635 4655 3015 Size: 826 794 20 4685 Fragments arranged by size: 4685 826 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6290 DraI TTT'AAA Cuts at: 3629 3629 Size: 6325 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5332 809 Size: 507 4016 1802 Fragments arranged by size: 4016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3772 4661 6290 Size: 54 1425 50 275 18 1942 889 1629 Cuts at: 6290 8 Size: 43 Fragments arranged by size: 1942 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3772 1537 Size: 275 1960 4090 Fragments arranged by size: 4090 1960 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2183 4255 1353 Size: 830 2072 3423 Fragments arranged by size: 3423 2072 830 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4912 5286 5432 387 Size: 1064 138 3323 374 146 1280 Fragments arranged by size: 3323 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2174 2345 2558 2563 2623 2839 Size: 393 405 137 171 213 5 60 216 Cuts at: 2839 3219 3461 3542 4388 4985 5772 6040 1239 Size: 380 242 81 846 597 787 268 1524 Fragments arranged by size: 1524 846 787 597 405 393 380 268 242 216 213 171 137 81 60 5 EcoO109I rG'GnC_Cy Cuts at: 2357 2358 3241 3353 3354 3534 2357 Size: 1 883 112 1 180 5148 Fragments arranged by size: 5148 883 180 112 1 1 EcoRI G'AATT_C Cuts at: 2470 3634 4012 2470 Size: 1164 378 4783 Fragments arranged by size: 4783 1164 378 FspI TGC'GCA Cuts at: 4831 4831 Size: 6325 HaeII r_GCGC'y Cuts at: 1678 2453 3195 5472 1678 Size: 775 742 2277 2531 Fragments arranged by size: 2531 2277 775 742 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2318 2350 3212 3244 3371 Size: 32 135 32 746 32 862 32 127 Cuts at: 3371 3403 4966 4998 5040 5072 1373 Size: 32 1563 32 42 32 2626 Fragments arranged by size: 2626 1563 862 746 135 127 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3669 3915 669 Size: 988 281 1731 246 3079 Fragments arranged by size: 3079 1731 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6325 HpaI GTT'AAC Cuts at: 3915 3915 Size: 6325 KasI G'GCGC_C Cuts at: 2449 3191 2449 Size: 742 5583 KpnI G_GTAC'C Cuts at: 3650 3650 Size: 6325 MfeI C'AATT_G Cuts at: 3924 3924 Size: 6325 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3203 3231 5439 5623 1305 Size: 1898 28 2208 184 2007 Fragments arranged by size: 2208 2007 1898 184 28 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4503 Fragments arranged by size: 4503 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2909 3116 4283 4642 4801 518 Size: 648 1743 207 1167 359 159 2042 Fragments arranged by size: 2042 1743 1167 648 359 207 159 MspA1I CmG'CkG Cuts at: 1606 2194 2400 2583 3741 4418 5566 5811 1606 Size: 588 206 183 1158 677 1148 245 2120 Fragments arranged by size: 2120 1158 1148 677 588 245 206 183 NaeI GCC'GGC Cuts at: 3190 3190 Size: 6325 NarI GG'CG_CC Cuts at: 2450 3192 2450 Size: 742 5583 NcoI C'CATG_G Cuts at: 513 1065 3361 513 Size: 552 2296 3477 Fragments arranged by size: 3477 2296 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6325 NgoMIV G'CCGG_C Cuts at: 3188 3188 Size: 6325 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6325 NotI GC'GGCC_GC Cuts at: 3772 3772 Size: 6325 NspI r_CATG'y Cuts at: 1132 1229 3683 5228 1132 Size: 97 2454 1545 2229 Fragments arranged by size: 2454 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2709 3452 1348 Size: 1361 743 4221 Fragments arranged by size: 4221 1361 743 PciI A'CATG_T Cuts at: 5224 5224 Size: 6325 PflMI CCAn_nnn'nTGG Cuts at: 1108 2528 2657 2692 3439 1108 Size: 1420 129 35 747 3994 Fragments arranged by size: 3994 1420 747 129 35 PfoI T'CCnGG_A Cuts at: 2436 3438 3507 2436 Size: 1002 69 5254 Fragments arranged by size: 5254 1002 69 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PmlI CAC'GTG Cuts at: 3412 3412 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4394 4426 5907 5939 4394 Size: 32 1481 32 4780 Fragments arranged by size: 4780 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6325 PsiI TTA'TAA Cuts at: 3895 3895 Size: 6325 PspOMI G'GGCC_C Cuts at: 2357 3353 2357 Size: 996 5329 PstI C_TGCA'G Cuts at: 830 2405 2490 2797 2806 3677 830 Size: 1575 85 307 9 871 3478 Fragments arranged by size: 3478 1575 871 307 85 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 PvuII CAG'CTG Cuts at: 2400 2400 Size: 6325 RsrII CG'GwC_CG Cuts at: 2305 2305 Size: 6325 SalI G'TCGA_C Cuts at: 1936 3667 1936 Size: 1731 4594 SbfI CC_TGCA'GG Cuts at: 3677 3677 Size: 6325 ScaI AGT'ACT Cuts at: 2216 4573 6285 2216 Size: 2357 1712 2256 Fragments arranged by size: 2357 2256 1712 SfcI C'TryA_G Cuts at: 826 1045 2401 2486 2793 2802 3673 4808 5489 Size: 219 1356 85 307 9 871 1135 681 Cuts at: 5489 5680 826 Size: 191 1471 Fragments arranged by size: 1471 1356 1135 871 681 307 219 191 85 9 SfoI GGC'GCC Cuts at: 2451 3193 2451 Size: 742 5583 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 3188 1700 Size: 1488 4837 SmaI CCC'GGG Cuts at: 1872 3652 1872 Size: 1780 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4429 5330 5592 820 Size: 197 926 2486 901 262 1553 Fragments arranged by size: 2486 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 3683 1132 Size: 2551 3774 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6325 StyI C'CwwG_G Cuts at: 513 1065 2513 3114 3244 3342 3361 3764 513 Size: 552 1448 601 130 98 19 403 3074 Fragments arranged by size: 3074 1448 601 552 403 130 98 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2197 4352 4369 4522 4707 5152 325 Size: 1255 617 2155 17 153 185 445 1498 Fragments arranged by size: 2155 1498 1255 617 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 4571 6283 Size: 274 80 33 51 1443 237 2357 1712 Cuts at: 6283 96 Size: 138 Fragments arranged by size: 2357 1712 1443 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6285 6317 6285 Size: 32 6293 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6325 XbaI T'CTAG_A Cuts at: 3661 3661 Size: 6325 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3340 3340 Size: 6325 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6325 XmaI C'CCGG_G Cuts at: 1870 3650 1870 Size: 1780 4545 XmnI GAAnn'nnTTC Cuts at: 4454 4454 Size: 6325 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SrfI SwaI