(Circular) (Six-base) MAPSORT of: pFN21AA1170.seq Check: 8244 from: 1 to: 6979 pFN21AA1170 6979 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4789 278 Size: 53 83 186 936 3253 2468 Fragments arranged by size: 3253 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4322 1937 Size: 2385 4594 Acc65I G'GTAC_C Cuts at: 3493 4300 3493 Size: 807 6172 AclI AA'CG_TT Cuts at: 1563 5106 5479 1563 Size: 3543 373 3063 Fragments arranged by size: 3543 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 2937 3854 5042 6426 6694 1632 Size: 767 538 917 1188 1384 268 1917 Fragments arranged by size: 1917 1384 1188 917 767 538 268 AflII C'TTAA_G Cuts at: 820 1017 3566 820 Size: 197 2549 4233 Fragments arranged by size: 4233 2549 197 AflIII A'CryG_T Cuts at: 1203 5878 6855 1203 Size: 4675 977 1327 Fragments arranged by size: 4675 1327 977 AgeI A'CCGG_T Cuts at: 5810 5810 Size: 6979 AhdI GACnn_n'nnGTC Cuts at: 5708 5708 Size: 6979 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6979 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6979 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3974 4006 3974 Size: 32 6947 AlwNI CAG_nnn'CTG Cuts at: 4722 6294 4722 Size: 1572 5407 ApaI G_GGCC'C Cuts at: 2039 3268 4096 2039 Size: 1229 828 4922 Fragments arranged by size: 4922 1229 828 ApaLI G'TGCA_C Cuts at: 5036 6192 5036 Size: 1156 5823 ApoI r'AATT_y Cuts at: 1493 2231 4288 4514 4666 6775 1493 Size: 738 2057 226 152 2109 1697 Fragments arranged by size: 2109 2057 1697 738 226 152 AseI AT'TA_AT Cuts at: 160 1032 5533 160 Size: 872 4501 1606 Fragments arranged by size: 4501 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6979 AvaI C'yCGr_G Cuts at: 1870 1943 2388 2685 4304 1870 Size: 73 445 297 1619 4545 Fragments arranged by size: 4545 1619 445 297 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3345 3378 3345 Size: 33 6946 BamHI G'GATC_C Cuts at: 4309 4676 4309 Size: 367 6612 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2998 3196 3217 3465 Size: 325 217 627 299 912 198 21 248 Cuts at: 3465 3493 4075 4167 4300 5755 618 Size: 28 582 92 133 1455 1842 Fragments arranged by size: 1842 1455 912 627 582 325 299 248 217 198 133 92 28 21 BanII G_rGCy'C Cuts at: 1391 2039 2138 2601 3111 3268 4096 4298 1391 Size: 648 99 463 510 157 828 202 4072 Fragments arranged by size: 4072 828 648 510 463 202 157 99 BbeI G_GCGC'C Cuts at: 2090 4171 2090 Size: 2081 4898 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2925 3401 4257 928 Size: 971 1026 476 856 3650 Fragments arranged by size: 3650 1026 971 856 476 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5170 5204 1903 Size: 34 3233 34 3678 Fragments arranged by size: 3678 3233 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2936 4871 6081 2936 Size: 1935 1210 3834 Fragments arranged by size: 3834 1935 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6979 BfrBI ATG'CAT Cuts at: 2722 2722 Size: 6979 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2321 3030 5590 Size: 107 122 71 631 605 649 709 2560 Cuts at: 5590 136 Size: 1525 Fragments arranged by size: 2560 1525 709 649 631 605 122 107 71 BglII A'GATC_T Cuts at: 6974 6974 Size: 6979 BlpI GC'TnA_GC Cuts at: 3231 4396 3231 Size: 1165 5814 Bme1580I G_kGCm'C Cuts at: 1790 2039 2560 3268 3470 4096 5040 6196 1790 Size: 249 521 708 202 626 944 1156 2573 Fragments arranged by size: 2573 1156 944 708 626 521 249 202 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6979 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4252 4710 5668 Size: 416 53 83 399 24 2835 458 958 Cuts at: 5668 442 Size: 1753 Fragments arranged by size: 2835 1753 958 458 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6979 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 2318 2531 2669 3479 3650 3975 3997 Size: 938 141 213 138 810 171 325 22 Cuts at: 3997 5639 1239 Size: 1642 2579 Fragments arranged by size: 2579 1642 938 810 325 213 171 141 138 22 Bpu10I CC'TnA_GC Cuts at: 2600 2600 Size: 6979 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2259 4082 5104 5969 6267 2259 Size: 1823 1022 865 298 2971 Fragments arranged by size: 2971 1823 1022 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2684 5642 882 Size: 617 1185 2958 2219 Fragments arranged by size: 2958 2219 1185 617 BsaAI yAC'GTr Cuts at: 493 1204 4770 493 Size: 711 3566 2702 Fragments arranged by size: 3566 2702 711 BsaBI GATnn'nnATC Cuts at: 3653 6973 3653 Size: 3320 3659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2087 3503 4168 4786 Size: 53 83 186 936 554 1416 665 618 Cuts at: 4786 5168 5803 275 Size: 382 635 1451 Fragments arranged by size: 1451 1416 936 665 635 618 554 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5412 5810 6084 6231 6642 5412 Size: 398 274 147 411 5749 Fragments arranged by size: 5749 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2150 2180 1178 Size: 30 165 30 747 30 5977 Fragments arranged by size: 5977 747 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2741 2744 2778 3038 3119 3134 3143 3467 Size: 1555 3 34 260 81 15 9 324 Cuts at: 3467 3659 3737 1186 Size: 192 78 4428 Fragments arranged by size: 4428 1555 324 260 192 81 78 34 15 9 3 BseYI C'CCAG_C Cuts at: 3679 6182 6678 3679 Size: 2503 496 3980 Fragments arranged by size: 3980 2503 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 3455 1163 Size: 573 564 1155 4687 Fragments arranged by size: 4687 1155 573 564 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4429 5190 6218 1540 Size: 275 186 2428 761 1028 2301 Fragments arranged by size: 2428 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2601 3297 5040 5125 6196 2601 Size: 696 1743 85 1071 3384 Fragments arranged by size: 3384 1743 1071 696 85 BsmI GAATG_Cn' Cuts at: 3534 4490 4583 3534 Size: 956 93 5930 Fragments arranged by size: 5930 956 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3299 3494 3299 Size: 195 6784 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2138 2560 2601 3111 3268 3297 Size: 399 249 99 422 41 510 157 29 Cuts at: 3297 3470 4096 4298 5040 5125 6196 1391 Size: 173 626 202 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 626 510 422 399 249 202 173 157 99 85 41 29 BspEI T'CCGG_A Cuts at: 6642 6642 Size: 6979 BspHI T'CATG_A Cuts at: 4868 6598 4868 Size: 1730 5249 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3183 4334 844 Size: 2339 1151 3489 Fragments arranged by size: 3489 2339 1151 BsrBI CCG'CTC Cuts at: 1394 1823 3235 4866 1394 Size: 429 1412 1631 3507 Fragments arranged by size: 3507 1631 1412 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2059 5474 5648 65 Size: 1183 811 3415 174 1396 Fragments arranged by size: 3415 1396 1183 811 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2346 5623 5810 1700 Size: 110 536 3277 187 2869 Fragments arranged by size: 3277 2869 536 187 110 BsrGI T'GTAC_A Cuts at: 96 3534 96 Size: 3438 3541 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6979 BssSI C'ACGA_G Cuts at: 5039 6051 5039 Size: 1012 5967 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6979 BstBI TT'CG_AA Cuts at: 5783 5783 Size: 6979 BstEII G'GTnAC_C Cuts at: 1184 2761 1184 Size: 1577 5402 BstXI CCAn_nnnn'nTGG Cuts at: 2509 4057 4162 2509 Size: 1548 105 5326 Fragments arranged by size: 5326 1548 105 BstYI r'GATC_y Cuts at: 1972 3088 4309 4676 5061 5078 6530 6616 6628 Size: 1116 1221 367 385 17 1452 86 12 Cuts at: 6628 6974 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1221 1116 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3642 4007 3642 Size: 365 6614 BtgI C'CryG_G Cuts at: 513 1065 2004 2757 3028 513 Size: 552 939 753 271 4464 Fragments arranged by size: 4464 939 753 552 271 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3409 487 Size: 683 56 2183 4057 Fragments arranged by size: 4057 2183 683 56 BtsI GCAGTG_nn' Cuts at: 2560 2782 3867 4495 5289 5309 2560 Size: 222 1085 628 794 20 4230 Fragments arranged by size: 4230 1085 794 628 222 20 ClaI AT'CG_AT Cuts at: 1554 3687 1554 Size: 2133 4846 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6944 DraI TTT'AAA Cuts at: 4069 4283 4069 Size: 214 6765 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5986 809 Size: 507 4670 1802 Fragments arranged by size: 4670 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4426 5315 6944 Size: 54 1425 50 275 18 2596 889 1629 Cuts at: 6944 8 Size: 43 Fragments arranged by size: 2596 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4426 1537 Size: 275 2614 4090 Fragments arranged by size: 4090 2614 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2933 3098 3120 3444 3480 4909 1353 Size: 1580 165 22 324 36 1429 3423 Fragments arranged by size: 3423 1580 1429 324 165 36 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5566 5940 6086 387 Size: 1064 138 3977 374 146 1280 Fragments arranged by size: 3977 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2599 2599 Size: 6979 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2318 2399 2531 2669 2937 3479 Size: 393 545 141 81 132 138 268 542 Cuts at: 3479 3650 3854 3975 3997 5042 5639 6426 6694 Size: 171 204 121 22 1045 597 787 268 Cuts at: 6694 1239 Size: 1524 Fragments arranged by size: 1524 1045 787 597 545 542 393 268 268 204 171 141 138 132 121 81 22 EcoNI CCTnn'n_nnAGG Cuts at: 3341 3521 3641 3341 Size: 180 120 6679 Fragments arranged by size: 6679 180 120 EcoO109I rG'GnC_Cy Cuts at: 2011 2035 2493 2809 3187 3253 3265 4093 2011 Size: 24 458 316 378 66 12 828 4897 Fragments arranged by size: 4897 828 458 378 316 66 24 12 EcoRI G'AATT_C Cuts at: 2231 4288 4666 2231 Size: 2057 378 4544 Fragments arranged by size: 4544 2057 378 FspI TGC'GCA Cuts at: 5485 5485 Size: 6979 HaeII r_GCGC'y Cuts at: 1678 2090 4171 6126 1678 Size: 412 2081 1955 2531 Fragments arranged by size: 2531 2081 1955 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5620 5652 5694 5726 1373 Size: 32 135 32 4048 32 42 32 2626 Fragments arranged by size: 4048 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4323 4569 669 Size: 988 281 2385 246 3079 Fragments arranged by size: 3079 2385 988 281 246 HindIII A'AGCT_T Cuts at: 748 2710 748 Size: 1962 5017 HpaI GTT'AAC Cuts at: 4569 4569 Size: 6979 KasI G'GCGC_C Cuts at: 2086 4167 2086 Size: 2081 4898 KpnI G_GTAC'C Cuts at: 3497 4304 3497 Size: 807 6172 MfeI C'AATT_G Cuts at: 4578 4578 Size: 6979 MluI A'CGCG_T Cuts at: 6855 6855 Size: 6979 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2283 3343 6093 6277 1305 Size: 978 1060 2750 184 2007 Fragments arranged by size: 2750 2007 1060 978 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5157 Fragments arranged by size: 5157 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3387 3689 4937 5296 5455 518 Size: 648 2221 302 1248 359 159 2042 Fragments arranged by size: 2221 2042 1248 648 359 302 159 MspA1I CmG'CkG Cuts at: 1606 2119 2573 2995 4395 5072 6220 6465 1606 Size: 513 454 422 1400 677 1148 245 2120 Fragments arranged by size: 2120 1400 1148 677 513 454 422 245 NarI GG'CG_CC Cuts at: 2087 4168 2087 Size: 2081 4898 NcoI C'CATG_G Cuts at: 513 1065 2004 2757 513 Size: 552 939 753 4735 Fragments arranged by size: 4735 939 753 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6979 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6979 NotI GC'GGCC_GC Cuts at: 4426 4426 Size: 6979 NsiI A_TGCA'T Cuts at: 2724 2724 Size: 6979 NspI r_CATG'y Cuts at: 1132 1229 2189 2947 4337 5882 1132 Size: 97 960 758 1390 1545 2229 Fragments arranged by size: 2229 1545 1390 960 758 97 PasI CC'CwG_GG Cuts at: 1348 3261 3337 1348 Size: 1913 76 4990 Fragments arranged by size: 4990 1913 76 PciI A'CATG_T Cuts at: 5878 5878 Size: 6979 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6979 PfoI T'CCnGG_A Cuts at: 2508 3891 3915 4043 2508 Size: 1383 24 128 5444 Fragments arranged by size: 5444 1383 128 24 PmeI GTTT'AAAC Cuts at: 4283 4283 Size: 6979 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5048 5080 6561 6593 5048 Size: 32 1481 32 5434 Fragments arranged by size: 5434 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 2493 2809 3187 2011 Size: 482 316 378 5803 Fragments arranged by size: 5803 482 378 316 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6979 PsiI TTA'TAA Cuts at: 4549 4549 Size: 6979 PspOMI G'GGCC_C Cuts at: 2035 3264 4092 2035 Size: 1229 828 4922 Fragments arranged by size: 4922 1229 828 PstI C_TGCA'G Cuts at: 830 2777 2868 3586 4331 830 Size: 1947 91 718 745 3478 Fragments arranged by size: 3478 1947 745 718 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6979 PvuII CAG'CTG Cuts at: 2573 2995 2573 Size: 422 6557 SacI G_AGCT'C Cuts at: 2601 2601 Size: 6979 SalI G'TCGA_C Cuts at: 1936 4321 1936 Size: 2385 4594 SapI GCTCTTCn'nnn_ Cuts at: 2933 2933 Size: 6979 SbfI CC_TGCA'GG Cuts at: 4331 4331 Size: 6979 ScaI AGT'ACT Cuts at: 5227 6939 5227 Size: 1712 5267 SfcI C'TryA_G Cuts at: 826 1045 2548 2773 2864 3582 4327 5462 6143 Size: 219 1503 225 91 718 745 1135 681 Cuts at: 6143 6334 826 Size: 191 1471 Fragments arranged by size: 1503 1471 1135 745 718 681 225 219 191 91 SfoI GGC'GCC Cuts at: 2088 4169 2088 Size: 2081 4898 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6979 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6979 SmaI CCC'GGG Cuts at: 1872 2390 2687 4306 1872 Size: 518 297 1619 4545 Fragments arranged by size: 4545 1619 518 297 SmlI C'TyrA_G Cuts at: 820 1017 1943 2274 3566 4097 5083 5984 6246 Size: 197 926 331 1292 531 986 901 262 Cuts at: 6246 820 Size: 1553 Fragments arranged by size: 1553 1292 986 926 901 531 331 262 197 SnaBI TAC'GTA Cuts at: 493 4770 493 Size: 4277 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6979 SphI G_CATG'C Cuts at: 1132 2189 2947 4337 1132 Size: 1057 758 1390 3774 Fragments arranged by size: 3774 1390 1057 758 SrfI GCCC'GGGC Cuts at: 2390 2390 Size: 6979 SspI AAT'ATT Cuts at: 5 52 2641 4903 5 Size: 47 2589 2262 2081 Fragments arranged by size: 2589 2262 2081 47 StuI AGG'CCT Cuts at: 1517 2324 2546 1517 Size: 807 222 5950 Fragments arranged by size: 5950 807 222 StyI C'CwwG_G Cuts at: 513 1065 2004 2091 2410 2757 3156 3249 4418 Size: 552 939 87 319 347 399 93 1169 Cuts at: 4418 513 Size: 3074 Fragments arranged by size: 3074 1169 939 552 399 347 319 93 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2661 5006 5023 5176 5361 5806 325 Size: 1255 1081 2345 17 153 185 445 1498 Fragments arranged by size: 2345 1498 1255 1081 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2878 3534 5225 Size: 274 80 33 51 1443 901 656 1691 Cuts at: 5225 6937 96 Size: 1712 138 Fragments arranged by size: 1712 1691 1443 901 656 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6939 6971 6939 Size: 32 6947 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6979 XbaI T'CTAG_A Cuts at: 4315 4315 Size: 6979 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2417 2417 Size: 6979 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6979 XmaI C'CCGG_G Cuts at: 1870 2388 2685 4304 1870 Size: 518 297 1619 4545 Fragments arranged by size: 4545 1619 518 297 XmnI GAAnn'nnTTC Cuts at: 2640 3320 3839 5108 2640 Size: 680 519 1269 4511 Fragments arranged by size: 4511 1269 680 519 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4787 276 Size: 53 83 186 936 3253 2468 Fragments arranged by size: 3253 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbvCI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SwaI