(Circular) (Six-base) MAPSORT of: pFN21AA1041.seq Check: 2000 from: 1 to: 6571 pFN21AA1041 6571 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4381 278 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3438 3914 1937 Size: 1501 476 4594 Fragments arranged by size: 4594 1501 476 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3672 4634 6018 6286 1632 Size: 2040 962 1384 268 1917 Fragments arranged by size: 2040 1917 1384 962 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 5470 6447 1203 Size: 4267 977 1327 Fragments arranged by size: 4267 1327 977 AgeI A'CCGG_T Cuts at: 2761 5402 2761 Size: 2641 3930 AhdI GACnn_n'nnGTC Cuts at: 5300 5300 Size: 6571 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6571 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6571 AlwNI CAG_nnn'CTG Cuts at: 4314 5886 4314 Size: 1572 4999 ApaLI G'TGCA_C Cuts at: 4628 5784 4628 Size: 1156 5415 ApoI r'AATT_y Cuts at: 1493 2160 2313 2374 3652 3880 4106 4258 6367 Size: 667 153 61 1278 228 226 152 2109 Cuts at: 6367 1493 Size: 1697 Fragments arranged by size: 2109 1697 1278 667 228 226 153 152 61 AseI AT'TA_AT Cuts at: 160 1032 2277 5125 160 Size: 872 1245 2848 1606 Fragments arranged by size: 2848 1606 1245 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 3896 1870 Size: 73 1953 4545 Fragments arranged by size: 4545 1953 73 BamHI G'GATC_C Cuts at: 3901 4268 3901 Size: 367 6204 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3319 3608 3892 5347 618 Size: 325 217 627 1532 289 284 1455 1842 Fragments arranged by size: 1842 1532 1455 627 325 289 284 217 BanII G_rGCy'C Cuts at: 1391 3890 1391 Size: 2499 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5600 BbvCI CC'TCA_GC Cuts at: 2805 2805 Size: 6571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4762 4796 1903 Size: 34 2825 34 3678 Fragments arranged by size: 3678 2825 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3352 4463 5673 3352 Size: 1111 1210 4250 Fragments arranged by size: 4250 1210 1111 BclI T'GATC_A Cuts at: 1548 2653 1548 Size: 1105 5466 BfrBI ATG'CAT Cuts at: 3616 3616 Size: 6571 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2500 5182 136 Size: 107 122 71 631 605 828 2682 1525 Fragments arranged by size: 2682 1525 828 631 605 122 107 71 BglII A'GATC_T Cuts at: 2875 3484 6566 2875 Size: 609 3082 2880 Fragments arranged by size: 3082 2880 609 BlpI GC'TnA_GC Cuts at: 3988 3988 Size: 6571 Bme1580I G_kGCm'C Cuts at: 1790 2518 4632 5788 1790 Size: 728 2114 1156 2573 Fragments arranged by size: 2573 2114 1156 728 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6571 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2467 4302 5260 Size: 416 53 83 399 24 1050 1835 958 Cuts at: 5260 442 Size: 1753 Fragments arranged by size: 1835 1753 1050 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 3218 3723 3813 5231 1239 Size: 845 1134 505 90 1418 2579 Fragments arranged by size: 2579 1418 1134 845 505 90 Bpu10I CC'TnA_GC Cuts at: 2805 2805 Size: 6571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3473 4696 5561 5859 3473 Size: 1223 865 298 4185 Fragments arranged by size: 4185 1223 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3112 5234 882 Size: 617 1613 2122 2219 Fragments arranged by size: 2219 2122 1613 617 BsaAI yAC'GTr Cuts at: 493 1204 4362 493 Size: 711 3158 2702 Fragments arranged by size: 3158 2702 711 BsaBI GATnn'nnATC Cuts at: 2948 6565 2948 Size: 3617 2954 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4378 4760 5395 275 Size: 53 83 186 936 2845 382 635 1451 Fragments arranged by size: 2845 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2761 5004 5402 5676 5823 6234 2761 Size: 2243 398 274 147 411 3098 Fragments arranged by size: 3098 2243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6346 Fragments arranged by size: 6346 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2793 1186 Size: 1607 4964 BseYI C'CCAG_C Cuts at: 2967 3005 3202 3249 5774 6270 2967 Size: 38 197 47 2525 496 3268 Fragments arranged by size: 3268 2525 496 197 47 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5998 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3164 4021 4782 5810 1540 Size: 275 186 1163 857 761 1028 2301 Fragments arranged by size: 2301 1163 1028 857 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3023 4632 4717 5788 3023 Size: 1609 85 1071 3806 Fragments arranged by size: 3806 1609 1071 85 BsmI GAATG_Cn' Cuts at: 2179 4082 4175 2179 Size: 1903 93 4575 Fragments arranged by size: 4575 1903 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2518 3023 3890 4632 4717 5788 1391 Size: 399 728 505 867 742 85 1071 2174 Fragments arranged by size: 2174 1071 867 742 728 505 399 85 BspEI T'CCGG_A Cuts at: 6234 6234 Size: 6571 BspHI T'CATG_A Cuts at: 4460 6190 4460 Size: 1730 4841 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3926 844 Size: 3082 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4458 1394 Size: 429 2635 3507 Fragments arranged by size: 3507 2635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5066 5240 65 Size: 1183 3818 174 1396 Fragments arranged by size: 3818 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2761 5215 5402 1700 Size: 110 951 2454 187 2869 Fragments arranged by size: 2869 2454 951 187 110 BsrGI T'GTAC_A Cuts at: 96 3657 96 Size: 3561 3010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6571 BssSI C'ACGA_G Cuts at: 4631 5643 4631 Size: 1012 5559 BstAPI GCAn_nnn'nTGC Cuts at: 794 3790 794 Size: 2996 3575 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 2091 1184 Size: 907 5664 BstXI CCAn_nnnn'nTGG Cuts at: 3641 3641 Size: 6571 BstYI r'GATC_y Cuts at: 1972 2875 3484 3901 4268 4653 4670 6122 6208 Size: 903 609 417 367 385 17 1452 86 Cuts at: 6208 6220 6566 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 903 609 417 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5080 Fragments arranged by size: 5080 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5832 Fragments arranged by size: 5832 683 56 BtsI GCAGTG_nn' Cuts at: 2368 3073 3424 4087 4881 4901 2368 Size: 705 351 663 794 20 4038 Fragments arranged by size: 4038 794 705 663 351 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6536 DraI TTT'AAA Cuts at: 3875 3875 Size: 6571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2025 5578 809 Size: 507 709 3553 1802 Fragments arranged by size: 3553 1802 709 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2501 4018 4907 Size: 54 1425 50 275 18 671 1517 889 Cuts at: 4907 6536 8 Size: 1629 43 Fragments arranged by size: 1629 1517 1425 889 671 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4018 1537 Size: 275 2206 4090 Fragments arranged by size: 4090 2206 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2504 4501 1353 Size: 1151 1997 3423 Fragments arranged by size: 3423 1997 1151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5158 5532 5678 387 Size: 1064 138 3569 374 146 1280 Fragments arranged by size: 3569 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 3218 3672 3723 3813 4634 5231 Size: 393 452 1134 454 51 90 821 597 Cuts at: 5231 6018 6286 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1134 821 787 597 454 452 393 268 90 51 EcoO109I rG'GnC_Cy Cuts at: 2451 2451 Size: 6571 EcoRI G'AATT_C Cuts at: 2374 3880 4258 2374 Size: 1506 378 4687 Fragments arranged by size: 4687 1506 378 FspI TGC'GCA Cuts at: 5077 5077 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 5718 1678 Size: 4040 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2103 2135 2758 2790 5212 Size: 32 135 32 531 32 623 32 2422 Cuts at: 5212 5244 5286 5318 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2422 623 531 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2933 3309 3915 4161 669 Size: 988 281 995 376 606 246 3079 Fragments arranged by size: 3079 995 988 606 376 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6571 HpaI GTT'AAC Cuts at: 3309 4161 3309 Size: 852 5719 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 3864 4170 3864 Size: 306 6265 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2242 2348 2627 2719 3078 3644 5685 5869 Size: 937 106 279 92 359 566 2041 184 Cuts at: 5869 1305 Size: 2007 Fragments arranged by size: 2041 2007 937 566 359 279 184 106 92 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4749 Fragments arranged by size: 4749 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2229 3066 4529 4888 5047 518 Size: 648 1063 837 1463 359 159 2042 Fragments arranged by size: 2042 1463 1063 837 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3987 4664 5812 6057 1606 Size: 2381 677 1148 245 2120 Fragments arranged by size: 2381 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5080 Fragments arranged by size: 5080 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6571 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6571 NotI GC'GGCC_GC Cuts at: 4018 4018 Size: 6571 NsiI A_TGCA'T Cuts at: 3618 3618 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 3929 5474 1132 Size: 97 2700 1545 2229 Fragments arranged by size: 2700 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6571 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 2713 3704 1108 Size: 1605 991 3975 Fragments arranged by size: 3975 1605 991 PfoI T'CCnGG_A Cuts at: 3739 3840 3739 Size: 101 6470 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6153 6185 4640 Size: 32 1481 32 5026 Fragments arranged by size: 5026 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2451 2451 Size: 6571 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6571 PsiI TTA'TAA Cuts at: 2904 4141 2904 Size: 1237 5334 PstI C_TGCA'G Cuts at: 830 3923 830 Size: 3093 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SbfI CC_TGCA'GG Cuts at: 3923 3923 Size: 6571 ScaI AGT'ACT Cuts at: 4819 6531 4819 Size: 1712 4859 SexAI A'CCwGG_T Cuts at: 3507 3507 Size: 6571 SfcI C'TryA_G Cuts at: 826 1045 2523 3919 5054 5735 5926 826 Size: 219 1478 1396 1135 681 191 1471 Fragments arranged by size: 1478 1471 1396 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 2761 1700 Size: 1061 5510 SmaI CCC'GGG Cuts at: 1872 3898 1872 Size: 2026 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3488 4675 5576 5838 820 Size: 197 926 1545 1187 901 262 1553 Fragments arranged by size: 1553 1545 1187 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4362 493 Size: 3869 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 3929 1132 Size: 2797 3774 SspI AAT'ATT Cuts at: 5 52 4495 5 Size: 47 4443 2081 Fragments arranged by size: 4443 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6571 StyI C'CwwG_G Cuts at: 513 1065 2004 4010 513 Size: 552 939 2006 3074 Fragments arranged by size: 3074 2006 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2302 3042 3147 3216 3228 4598 4615 Size: 1255 722 740 105 69 12 1370 17 Cuts at: 4615 4768 4953 5398 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1370 1255 740 722 445 185 153 105 69 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2820 3011 3657 Size: 274 80 33 51 1443 843 191 646 Cuts at: 3657 4817 6529 96 Size: 1160 1712 138 Fragments arranged by size: 1712 1443 1160 843 646 274 191 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6531 6563 6531 Size: 32 6539 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6571 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3164 3164 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6571 XmaI C'CCGG_G Cuts at: 1870 3896 1870 Size: 2026 4545 XmnI GAAnn'nnTTC Cuts at: 4700 4700 Size: 6571 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4379 276 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI