(Circular) (Six-base) MAPSORT of: pFN21AA1035.seq Check: 5338 from: 1 to: 8383 pFN21AA1035 8383 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4448 4448 Size: 8383 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6193 278 Size: 53 83 186 936 4657 2468 Fragments arranged by size: 4657 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2727 3479 5726 1937 Size: 790 752 2247 4594 Fragments arranged by size: 4594 2247 790 752 Acc65I G'GTAC_C Cuts at: 5360 5704 5360 Size: 344 8039 AclI AA'CG_TT Cuts at: 1563 4802 6510 6883 1563 Size: 3239 1708 373 3063 Fragments arranged by size: 3239 3063 1708 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2085 2528 3108 3822 6446 7830 8098 1632 Size: 453 443 580 714 2624 1384 268 1917 Fragments arranged by size: 2624 1917 1384 714 580 453 443 268 AflII C'TTAA_G Cuts at: 820 1017 2263 820 Size: 197 1246 6940 Fragments arranged by size: 6940 1246 197 AflIII A'CryG_T Cuts at: 1203 5260 7282 8259 1203 Size: 4057 2022 977 1327 Fragments arranged by size: 4057 2022 1327 977 AgeI A'CCGG_T Cuts at: 7214 7214 Size: 8383 AhdI GACnn_n'nnGTC Cuts at: 7112 7112 Size: 8383 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8383 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3264 3296 3747 3779 5529 5561 3264 Size: 32 451 32 1750 32 6086 Fragments arranged by size: 6086 1750 451 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2114 4454 6126 7698 2114 Size: 2340 1672 1572 2799 Fragments arranged by size: 2799 2340 1672 1572 ApaLI G'TGCA_C Cuts at: 6440 7596 6440 Size: 1156 7227 ApoI r'AATT_y Cuts at: 1493 2136 2208 2447 2494 2509 2578 2650 2817 Size: 643 72 239 47 15 69 72 167 Cuts at: 2817 2892 3181 3406 3826 4136 4148 5390 5692 Size: 75 289 225 420 310 12 1242 302 Cuts at: 5692 5918 6070 8179 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1242 643 420 310 302 289 239 226 225 167 152 75 72 72 69 47 15 12 AseI AT'TA_AT Cuts at: 160 1032 2469 6937 160 Size: 872 1437 4468 1606 Fragments arranged by size: 4468 1606 1437 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8383 AvaI C'yCGr_G Cuts at: 1870 1943 4755 5364 5708 1870 Size: 73 2812 609 344 4545 Fragments arranged by size: 4545 2812 609 344 73 BamHI G'GATC_C Cuts at: 2938 5713 6080 2938 Size: 2775 367 5241 Fragments arranged by size: 5241 2775 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2772 5360 5704 7159 618 Size: 325 217 627 985 2588 344 1455 1842 Fragments arranged by size: 2588 1842 1455 985 627 344 325 217 BanII G_rGCy'C Cuts at: 1391 2479 5702 1391 Size: 1088 3223 4072 Fragments arranged by size: 4072 3223 1088 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3674 928 Size: 971 1775 5637 Fragments arranged by size: 5637 1775 971 BbvCI CC'TCA_GC Cuts at: 4608 4608 Size: 8383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2504 2538 6574 6608 1903 Size: 34 567 34 4036 34 3678 Fragments arranged by size: 4036 3678 567 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2596 5149 5156 6275 7485 2596 Size: 2553 7 1119 1210 3494 Fragments arranged by size: 3494 2553 1210 1119 7 BclI T'GATC_A Cuts at: 1548 3547 5329 1548 Size: 1999 1782 4602 Fragments arranged by size: 4602 1999 1782 BfrBI ATG'CAT Cuts at: 4576 4576 Size: 8383 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6994 136 Size: 107 122 71 631 605 5322 1525 Fragments arranged by size: 5322 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2239 3256 8378 2239 Size: 1017 5122 2244 Fragments arranged by size: 5122 2244 1017 BlpI GC'TnA_GC Cuts at: 2094 3066 5800 2094 Size: 972 2734 4677 Fragments arranged by size: 4677 2734 972 Bme1580I G_kGCm'C Cuts at: 1790 6444 7600 1790 Size: 4654 1156 2573 Fragments arranged by size: 4654 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8383 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6114 7072 442 Size: 416 53 83 399 24 4697 958 1753 Fragments arranged by size: 4697 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8383 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2941 3810 4788 7043 1239 Size: 860 842 869 978 2255 2579 Fragments arranged by size: 2579 2255 978 869 860 842 Bpu10I CC'TnA_GC Cuts at: 3285 4608 4992 3285 Size: 1323 384 6676 Fragments arranged by size: 6676 1323 384 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4017 4423 5619 6508 7373 7671 4017 Size: 406 1196 889 865 298 4729 Fragments arranged by size: 4729 1196 889 865 406 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7046 882 Size: 617 5547 2219 Fragments arranged by size: 5547 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3362 4557 5261 6174 493 Size: 711 2158 1195 704 913 2702 Fragments arranged by size: 2702 2158 1195 913 711 704 BsaBI GATnn'nnATC Cuts at: 4971 8377 4971 Size: 3406 4977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6190 6572 7207 275 Size: 53 83 186 936 4657 382 635 1451 Fragments arranged by size: 4657 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4963 6816 7214 7488 7635 8046 4963 Size: 1853 398 274 147 411 5300 Fragments arranged by size: 5300 1853 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2985 3015 3858 3888 1178 Size: 30 165 30 1582 30 843 30 5673 Fragments arranged by size: 5673 1582 843 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 8383 BseYI C'CCAG_C Cuts at: 3228 5200 7586 8082 3228 Size: 1972 2386 496 3529 Fragments arranged by size: 3529 2386 1972 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3769 5589 1163 Size: 573 2033 1820 3957 Fragments arranged by size: 3957 2033 1820 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5833 6594 7622 1540 Size: 275 186 3832 761 1028 2301 Fragments arranged by size: 3832 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6444 6529 7600 6444 Size: 85 1071 7227 Fragments arranged by size: 7227 1071 85 BsmI GAATG_Cn' Cuts at: 2268 2368 4275 5894 5987 2268 Size: 100 1907 1619 93 4664 Fragments arranged by size: 4664 1907 1619 100 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2479 5702 6444 6529 7600 1391 Size: 399 689 3223 742 85 1071 2174 Fragments arranged by size: 3223 2174 1071 742 689 399 85 BspEI T'CCGG_A Cuts at: 4963 8046 4963 Size: 3083 5300 BspHI T'CATG_A Cuts at: 4815 6272 8002 4815 Size: 1457 1730 5196 Fragments arranged by size: 5196 1730 1457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4448 5738 844 Size: 3604 1290 3489 Fragments arranged by size: 3604 3489 1290 BsrBI CCG'CTC Cuts at: 1394 1823 6270 1394 Size: 429 4447 3507 Fragments arranged by size: 4447 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2690 3617 4692 5171 6878 7052 65 Size: 1183 1442 927 1075 479 1707 174 1396 Fragments arranged by size: 1707 1442 1396 1183 1075 927 479 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2787 7027 7214 1700 Size: 110 977 4240 187 2869 Fragments arranged by size: 4240 2869 977 187 110 BsrGI T'GTAC_A Cuts at: 96 4012 96 Size: 3916 4467 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8383 BssSI C'ACGA_G Cuts at: 6443 7455 6443 Size: 1012 7371 BstAPI GCAn_nnn'nTGC Cuts at: 794 2512 4379 794 Size: 1718 1867 4798 Fragments arranged by size: 4798 1867 1718 BstBI TT'CG_AA Cuts at: 7187 7187 Size: 8383 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8383 BstYI r'GATC_y Cuts at: 1972 2239 2938 3256 3490 5713 6080 6465 6482 Size: 267 699 318 234 2223 367 385 17 Cuts at: 6482 7934 8020 8032 8378 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2223 1977 1452 699 385 367 346 318 267 234 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2590 2590 Size: 8383 BtgI C'CryG_G Cuts at: 513 1065 3574 4388 5304 513 Size: 552 2509 814 916 3592 Fragments arranged by size: 3592 2509 916 814 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2507 4895 487 Size: 683 56 1281 2388 3975 Fragments arranged by size: 3975 2388 1281 683 56 BtsI GCAGTG_nn' Cuts at: 2112 4830 4952 5025 5899 6693 6713 2112 Size: 2718 122 73 874 794 20 3782 Fragments arranged by size: 3782 2718 874 794 122 73 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8383 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8348 DraI TTT'AAA Cuts at: 2623 2832 4864 5687 2623 Size: 209 2032 823 5319 Fragments arranged by size: 5319 2032 823 209 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7390 809 Size: 507 6074 1802 Fragments arranged by size: 6074 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5056 5830 6719 Size: 54 1425 50 275 18 3226 774 889 Cuts at: 6719 8348 8 Size: 1629 43 Fragments arranged by size: 3226 1629 1425 889 774 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5830 1537 Size: 275 4018 4090 Fragments arranged by size: 4090 4018 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2299 4113 5370 6313 1353 Size: 946 1814 1257 943 3423 Fragments arranged by size: 3423 1814 1257 946 943 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3960 6970 7344 7490 387 Size: 1064 138 2371 3010 374 146 1280 Fragments arranged by size: 3010 2371 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2099 2528 2941 3108 3810 3822 Size: 393 453 14 429 413 167 702 12 Cuts at: 3822 4788 6446 7043 7830 8098 1239 Size: 966 1658 597 787 268 1524 Fragments arranged by size: 1658 1524 966 787 702 597 453 429 413 393 268 167 14 12 EcoNI CCTnn'n_nnAGG Cuts at: 3384 3384 Size: 8383 EcoRI G'AATT_C Cuts at: 2578 2650 3406 5692 6070 2578 Size: 72 756 2286 378 4891 Fragments arranged by size: 4891 2286 756 378 72 EcoRV GAT'ATC Cuts at: 4066 4066 Size: 8383 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3499 5663 3499 Size: 2164 6219 FspI TGC'GCA Cuts at: 4169 6889 4169 Size: 2720 5663 HaeII r_GCGC'y Cuts at: 1678 7530 1678 Size: 5852 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 2724 2756 2923 Size: 32 135 32 698 32 422 32 167 Cuts at: 2923 2955 2985 3017 3305 3337 3383 3415 3431 Size: 32 30 32 288 32 46 32 16 Cuts at: 3431 3463 3806 3838 3858 3890 7024 7056 7098 Size: 32 343 32 20 32 3134 32 42 Cuts at: 7098 7130 1373 Size: 32 2626 Fragments arranged by size: 3134 2626 698 422 343 288 167 135 46 42 32 32 32 32 32 32 32 32 32 32 32 32 32 30 20 16 HincII GTy'rAC Cuts at: 669 1657 1938 2220 5727 5973 669 Size: 988 281 282 3507 246 3079 Fragments arranged by size: 3507 3079 988 282 281 246 HindIII A'AGCT_T Cuts at: 748 3776 748 Size: 3028 5355 HpaI GTT'AAC Cuts at: 5973 5973 Size: 8383 KpnI G_GTAC'C Cuts at: 5364 5708 5364 Size: 344 8039 MfeI C'AATT_G Cuts at: 5982 5982 Size: 8383 MluI A'CGCG_T Cuts at: 8259 8259 Size: 8383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4344 4785 7497 7681 1305 Size: 3039 441 2712 184 2007 Fragments arranged by size: 3039 2712 2007 441 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5058 10 Size: 54 1425 343 3226 3335 Fragments arranged by size: 3335 3226 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2778 4059 4518 5121 6341 6700 6859 Size: 648 1612 1281 459 603 1220 359 159 Cuts at: 6859 518 Size: 2042 Fragments arranged by size: 2042 1612 1281 1220 648 603 459 359 159 MspA1I CmG'CkG Cuts at: 1606 2291 4662 5220 5799 6476 7624 7869 1606 Size: 685 2371 558 579 677 1148 245 2120 Fragments arranged by size: 2371 2120 1148 685 677 579 558 245 NcoI C'CATG_G Cuts at: 513 1065 3574 4388 5304 513 Size: 552 2509 814 916 3592 Fragments arranged by size: 3592 2509 916 814 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8383 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8383 NotI GC'GGCC_GC Cuts at: 5830 5830 Size: 8383 NsiI A_TGCA'T Cuts at: 4578 4578 Size: 8383 NspI r_CATG'y Cuts at: 1132 1229 2803 3717 5741 7286 1132 Size: 97 1574 914 2024 1545 2229 Fragments arranged by size: 2229 2024 1574 1545 914 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8383 PciI A'CATG_T Cuts at: 7282 7282 Size: 8383 PflMI CCAn_nnn'nTGG Cuts at: 1108 4389 1108 Size: 3281 5102 PfoI T'CCnGG_A Cuts at: 4765 4765 Size: 8383 PmeI GTTT'AAAC Cuts at: 5687 5687 Size: 8383 PmlI CAC'GTG Cuts at: 5261 5261 Size: 8383 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6452 6484 7965 7997 6452 Size: 32 1481 32 6838 Fragments arranged by size: 6838 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3678 1613 Size: 2065 6318 PsiI TTA'TAA Cuts at: 2663 5953 2663 Size: 3290 5093 PstI C_TGCA'G Cuts at: 830 3661 3680 4459 5020 5735 830 Size: 2831 19 779 561 715 3478 Fragments arranged by size: 3478 2831 779 715 561 19 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8383 PvuII CAG'CTG Cuts at: 2291 4662 5220 2291 Size: 2371 558 5454 Fragments arranged by size: 5454 2371 558 SalI G'TCGA_C Cuts at: 1936 5725 1936 Size: 3789 4594 SbfI CC_TGCA'GG Cuts at: 5735 5735 Size: 8383 ScaI AGT'ACT Cuts at: 5314 5646 6631 8343 5314 Size: 332 985 1712 5354 Fragments arranged by size: 5354 1712 985 332 SexAI A'CCwGG_T Cuts at: 4276 4276 Size: 8383 SfcI C'TryA_G Cuts at: 826 1045 2196 2724 3073 3657 3676 4455 5016 Size: 219 1151 528 349 584 19 779 561 Cuts at: 5016 5221 5731 6866 7547 7738 826 Size: 205 510 1135 681 191 1471 Fragments arranged by size: 1471 1151 1135 779 681 584 561 528 510 349 219 205 191 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8383 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8383 SmaI CCC'GGG Cuts at: 1872 5710 1872 Size: 3838 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2263 4032 4438 5634 6487 7388 Size: 197 926 320 1769 406 1196 853 901 Cuts at: 7388 7650 820 Size: 262 1553 Fragments arranged by size: 1769 1553 1196 926 901 853 406 320 262 197 SnaBI TAC'GTA Cuts at: 493 4557 6174 493 Size: 4064 1617 2702 Fragments arranged by size: 4064 2702 1617 SpeI A'CTAG_T Cuts at: 152 152 Size: 8383 SphI G_CATG'C Cuts at: 1132 5741 1132 Size: 4609 3774 SspI AAT'ATT Cuts at: 5 52 2343 3628 3932 6307 5 Size: 47 2291 1285 304 2375 2081 Fragments arranged by size: 2375 2291 2081 1285 304 47 StuI AGG'CCT Cuts at: 1517 3602 4003 1517 Size: 2085 401 5897 Fragments arranged by size: 5897 2085 401 StyI C'CwwG_G Cuts at: 513 1065 3574 3603 4388 4668 5034 5304 5822 Size: 552 2509 29 785 280 366 270 518 Cuts at: 5822 513 Size: 3074 Fragments arranged by size: 3074 2509 785 552 518 366 280 270 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3624 6410 6427 6580 6765 7210 325 Size: 1255 2044 2786 17 153 185 445 1498 Fragments arranged by size: 2786 2044 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4012 5287 5312 Size: 274 80 33 51 1443 2035 1275 25 Cuts at: 5312 5644 6629 8341 96 Size: 332 985 1712 138 Fragments arranged by size: 2035 1712 1443 1275 985 332 274 138 80 51 33 25 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8343 8375 8343 Size: 32 8351 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8383 XbaI T'CTAG_A Cuts at: 5719 5719 Size: 8383 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5521 5521 Size: 8383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8383 XmaI C'CCGG_G Cuts at: 1870 5708 1870 Size: 3838 4545 XmnI GAAnn'nnTTC Cuts at: 2978 3562 6512 2978 Size: 584 2950 4849 Fragments arranged by size: 4849 2950 584 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6191 276 Size: 53 83 186 936 4657 2468 Fragments arranged by size: 4657 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI