Restriction map for
pFN21AA1017
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoT22I FauI FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AciI 2373 GCGG CCGC(-3/-1)
AflIII 1935 ACATGT A^C[GA][CT]GT
AocI 2961 CCTGAGG CC^T[ACGT]AGG
Aor51HI 731 AGCGCT AGC^GCT
ApaI 2877 GGGCCC GGGCC^C
BcnI 2216 CCCGG CC^[GC]GG
BglII 2142 AGATCT A^GATCT
BsiEI 3018 CGATCG CG[GA][CT]^CG
BsmI 1232 GCATTC GAATGC(1/-1)
Bsp1407I 195 TGTACA T^GTACA
BspLU11I 1935 ACATGT A^CATGT
BsrGI 195 TGTACA T^GTACA
Bsu36I 2961 CCTGAGG CC^T[ACGT]AGG
DraI 1099 TTTAAA TTT^AAA
Eco47III 731 AGCGCT AGC^GCT
Eco81I 2961 CCTGAGG CC^T[ACGT]AGG
HaeII 731 AGCGCT [GA]GCGC^[CT]
HapII 2217 CCGG C^CGG
HincII 181 GTTGAC GT[CT]^[GA]AC
HindII 181 GTTGAC GT[CT]^[GA]AC
HpaII 2217 CCGG C^CGG
MspI 2217 CCGG C^CGG
NciI 2216 CCCGG CC^[GC]GG
PvuI 3018 CGATCG CGAT^CG
SacI 1369 GAGCTC GAGCT^C
SapI 2245 GCTCTTC GCTCTTC(1/4)
SpeI 1459 ACTAGT A^CTAGT
SphI 3002 GCATGC GCATG^C
SspBI 195 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 27 enzymes) #
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Asp700I 335 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1213 GAAACTTTTC
BamHI 511 GGATCC G^GATCC
2720 GGATCC
BanI 880 GGCACC G^G[CT][GA]CC
1330 GGCACC
BciVI 51 GGATAC GTATCC(6/5)
2073 GGATAC
BclI 81 TGATCA T^GATCA
498 TGATCA
BsaI 1362 GAGACC GGTCTC(1/5)
2728 GAGACC
BsiYI 1422 CCCAAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2880 CCCAGATCGGG
BslI 1422 CCCAAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2880 CCCAGATCGGG
BssSI 1341 CACGAG CACGAG(-5/-1)
2778 CACGAG
BstAPI 136 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
558 GCATTGTGTGC
BstXI 2465 CCAGCACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2834 CCATCAATGTGG
CfoI 732 GCGC GCG^C
1838 GCGC
EcoRI 1105 GAATTC G^AATTC
1908 GAATTC
FbaI 81 TGATCA T^GATCA
498 TGATCA
HhaI 732 GCGC GCG^C
1838 GCGC
HinP1I 732 GCGC G^CGC
1838 GCGC
HindIII 2041 AAGCTT A^AGCTT
2746 AAGCTT
NcoI 876 CCATGG C^CATGG
2873 CCATGG
PpuMI 1127 AGGACCT [GA]G^G[AT]CC[CT]
1532 AGGACCC
ScaI 1958 AGTACT AGT^ACT
1997 AGTACT
SfaNI 2449 GATGC GCATC(5/9)
992 GCATC
SmlI 2386 CTTGAG C^T[CT][GA]AG
2923 CTTGAG
SspI 1704 AATATT AAT^ATT
2671 AATATT
TaqI 243 TCGA T^CGA
2250 TCGA
TthHB8I 243 TCGA T^CGA
2250 TCGA
XcmI 1928 CCAGTGGACATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2881 CCAGATCGGGCTTGG
XmnI 335 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1213 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII