(Circular) (Six-base) MAPSORT of: pFN21AA0983.seq Check: 1975 from: 1 to: 6754 pFN21AA0983 6754 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2186 2802 3167 2186 Size: 616 365 5773 Fragments arranged by size: 5773 616 365 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4564 278 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4097 1937 Size: 2160 4594 Acc65I G'GTAC_C Cuts at: 4075 4075 Size: 6754 AclI AA'CG_TT Cuts at: 1563 4881 5254 1563 Size: 3318 373 3063 Fragments arranged by size: 3318 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 2240 3047 3080 3461 3973 4027 4817 Size: 456 152 807 33 381 512 54 790 Cuts at: 4817 6201 6469 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 807 790 512 456 381 268 152 54 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6557 AflIII A'CryG_T Cuts at: 1203 5653 6630 1203 Size: 4450 977 1327 Fragments arranged by size: 4450 1327 977 AgeI A'CCGG_T Cuts at: 5585 5585 Size: 6754 AhdI GACnn_n'nnGTC Cuts at: 3187 5483 3187 Size: 2296 4458 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6754 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3038 1184 Size: 1854 4900 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2253 2285 2253 Size: 32 6722 AlwNI CAG_nnn'CTG Cuts at: 2197 2401 3343 3889 4497 6069 2197 Size: 204 942 546 608 1572 2882 Fragments arranged by size: 2882 1572 942 608 546 204 ApaI G_GGCC'C Cuts at: 3951 3951 Size: 6754 ApaLI G'TGCA_C Cuts at: 4811 5967 4811 Size: 1156 5598 ApoI r'AATT_y Cuts at: 1493 3956 4063 4289 4441 6550 1493 Size: 2463 107 226 152 2109 1697 Fragments arranged by size: 2463 2109 1697 226 152 107 AseI AT'TA_AT Cuts at: 160 1032 5308 160 Size: 872 4276 1606 Fragments arranged by size: 4276 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 AvaI C'yCGr_G Cuts at: 1870 1943 4079 1870 Size: 73 2136 4545 Fragments arranged by size: 4545 2136 73 BamHI G'GATC_C Cuts at: 2268 4084 4451 2268 Size: 1816 367 4571 Fragments arranged by size: 4571 1816 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3033 4075 5530 618 Size: 325 217 627 1246 1042 1455 1842 Fragments arranged by size: 1842 1455 1246 1042 627 325 217 BanII G_rGCy'C Cuts at: 1391 2062 3951 4073 1391 Size: 671 1889 122 4072 Fragments arranged by size: 4072 1889 671 122 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3449 928 Size: 971 1550 4233 Fragments arranged by size: 4233 1550 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4945 4979 1903 Size: 34 3008 34 3678 Fragments arranged by size: 3678 3008 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3228 4646 5856 3228 Size: 1418 1210 4126 Fragments arranged by size: 4126 1418 1210 BclI T'GATC_A Cuts at: 1548 3159 1548 Size: 1611 5143 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5365 136 Size: 107 122 71 631 605 3693 1525 Fragments arranged by size: 3693 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6749 6749 Size: 6754 BlpI GC'TnA_GC Cuts at: 2361 3404 4171 2361 Size: 1043 767 4944 Fragments arranged by size: 4944 1043 767 Bme1580I G_kGCm'C Cuts at: 1790 3683 3951 4815 5971 1790 Size: 1893 268 864 1156 2573 Fragments arranged by size: 2573 1893 1156 864 268 BmgBI CAC'GTC Cuts at: 1318 2573 1318 Size: 1255 5499 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2590 2999 4485 Size: 416 53 83 399 24 1173 409 1486 Cuts at: 4485 5443 442 Size: 958 1753 Fragments arranged by size: 1753 1486 1173 958 416 409 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6754 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2959 2959 Size: 6754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 2609 2675 2898 3935 5414 1239 Size: 1139 231 66 223 1037 1479 2579 Fragments arranged by size: 2579 1479 1139 1037 231 223 66 Bpu10I CC'TnA_GC Cuts at: 2681 2681 Size: 6754 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2636 3329 4879 5744 6042 2636 Size: 693 1550 865 298 3348 Fragments arranged by size: 3348 1550 865 693 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2541 2974 3416 3911 5417 882 Size: 617 1042 433 442 495 1506 2219 Fragments arranged by size: 2219 1506 1042 617 495 442 433 BsaAI yAC'GTr Cuts at: 493 1204 4545 493 Size: 711 3341 2702 Fragments arranged by size: 3341 2702 711 BsaBI GATnn'nnATC Cuts at: 2379 6748 2379 Size: 4369 2385 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4561 4943 5578 275 Size: 53 83 186 936 3028 382 635 1451 Fragments arranged by size: 3028 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2728 5187 5585 5859 6006 6417 2728 Size: 2459 398 274 147 411 3065 Fragments arranged by size: 3065 2459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 2729 2759 3959 Size: 30 165 30 1038 30 258 30 1200 Cuts at: 3959 3989 1178 Size: 30 3943 Fragments arranged by size: 3943 1200 1038 258 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2507 2969 2972 3715 1186 Size: 949 372 462 3 743 4225 Fragments arranged by size: 4225 949 743 462 372 3 BseYI C'CCAG_C Cuts at: 2062 3343 3375 3638 3854 5957 6453 2062 Size: 1281 32 263 216 2103 496 2363 Fragments arranged by size: 2363 2103 1281 496 263 216 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2753 3195 3356 3473 3587 1163 Size: 573 1017 442 161 117 114 4330 Fragments arranged by size: 4330 1017 573 442 161 117 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4204 4965 5993 1540 Size: 275 186 2203 761 1028 2301 Fragments arranged by size: 2301 2203 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4815 4900 5971 4815 Size: 85 1071 5598 Fragments arranged by size: 5598 1071 85 BsmI GAATG_Cn' Cuts at: 4265 4358 4265 Size: 93 6661 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 3683 3951 4073 4815 4900 5971 Size: 399 272 1621 268 122 742 85 1071 Cuts at: 5971 1391 Size: 2174 Fragments arranged by size: 2174 1621 1071 742 399 272 268 122 85 BspEI T'CCGG_A Cuts at: 6417 6417 Size: 6754 BspHI T'CATG_A Cuts at: 2289 3105 3120 4643 6373 2289 Size: 816 15 1523 1730 2670 Fragments arranged by size: 2670 1730 1523 816 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2186 2802 3167 4109 844 Size: 1342 616 365 942 3489 Fragments arranged by size: 3489 1342 942 616 365 BsrBI CCG'CTC Cuts at: 1394 1823 4641 1394 Size: 429 2818 3507 Fragments arranged by size: 3507 2818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5249 5423 65 Size: 1183 4001 174 1396 Fragments arranged by size: 4001 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5398 5585 1700 Size: 110 3588 187 2869 Fragments arranged by size: 3588 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6754 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6754 BssSI C'ACGA_G Cuts at: 2354 4814 5826 2354 Size: 2460 1012 3282 Fragments arranged by size: 3282 2460 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6754 BstBI TT'CG_AA Cuts at: 5558 5558 Size: 6754 BstEII G'GTnAC_C Cuts at: 1184 3317 1184 Size: 2133 4621 BstXI CCAn_nnnn'nTGG Cuts at: 2446 3418 2446 Size: 972 5782 BstYI r'GATC_y Cuts at: 1972 2134 2208 2268 4084 4451 4836 4853 6305 Size: 162 74 60 1816 367 385 17 1452 Cuts at: 6305 6391 6403 6749 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1816 1452 385 367 346 162 86 74 60 17 12 Bsu36I CC'TnA_GG Cuts at: 4032 4032 Size: 6754 BtgI C'CryG_G Cuts at: 513 1065 2541 3150 3804 513 Size: 552 1476 609 654 3463 Fragments arranged by size: 3463 1476 654 609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2067 4034 487 Size: 683 56 841 1967 3207 Fragments arranged by size: 3207 1967 841 683 56 BtsI GCAGTG_nn' Cuts at: 2829 2887 4270 5064 5084 2829 Size: 58 1383 794 20 4499 Fragments arranged by size: 4499 1383 794 58 20 ClaI AT'CG_AT Cuts at: 1554 2083 1554 Size: 529 6225 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6719 DraI TTT'AAA Cuts at: 4018 4058 4018 Size: 40 6714 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5761 809 Size: 507 4445 1802 Fragments arranged by size: 4445 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4201 5090 6719 Size: 54 1425 50 275 18 2371 889 1629 Cuts at: 6719 8 Size: 43 Fragments arranged by size: 2371 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4201 1537 Size: 275 2389 4090 Fragments arranged by size: 4090 2389 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 2335 3023 3539 3992 4684 1353 Size: 863 119 688 516 453 692 3423 Fragments arranged by size: 3423 863 692 688 516 453 119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5341 5715 5861 387 Size: 1064 138 3752 374 146 1280 Fragments arranged by size: 3752 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2240 2378 2609 2675 2898 3047 Size: 393 456 152 138 231 66 223 149 Cuts at: 3047 3080 3461 3935 3973 4027 4817 5414 6201 Size: 33 381 474 38 54 790 597 787 Cuts at: 6201 6469 1239 Size: 268 1524 Fragments arranged by size: 1524 790 787 597 474 456 393 381 268 231 223 152 149 138 66 54 38 33 EcoNI CCTnn'n_nnAGG Cuts at: 2426 2426 Size: 6754 EcoO109I rG'GnC_Cy Cuts at: 2143 3947 3995 2143 Size: 1804 48 4902 Fragments arranged by size: 4902 1804 48 EcoRI G'AATT_C Cuts at: 4063 4441 4063 Size: 378 6376 EcoRV GAT'ATC Cuts at: 2521 2521 Size: 6754 FspI TGC'GCA Cuts at: 5260 5260 Size: 6754 HaeII r_GCGC'y Cuts at: 1678 2977 3411 3668 5901 1678 Size: 1299 434 257 2233 2531 Fragments arranged by size: 2531 2233 1299 434 257 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2441 2473 2615 2647 3173 Size: 32 135 32 869 32 142 32 526 Cuts at: 3173 3205 5395 5427 5469 5501 1373 Size: 32 2190 32 42 32 2626 Fragments arranged by size: 2626 2190 869 526 142 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3870 4098 4344 669 Size: 988 281 1932 228 246 3079 Fragments arranged by size: 3079 1932 988 281 246 228 HindIII A'AGCT_T Cuts at: 748 2303 748 Size: 1555 5199 HpaI GTT'AAC Cuts at: 4344 4344 Size: 6754 KpnI G_GTAC'C Cuts at: 4079 4079 Size: 6754 MfeI C'AATT_G Cuts at: 3576 4353 3576 Size: 777 5977 MluI A'CGCG_T Cuts at: 6630 6630 Size: 6754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2982 5868 6052 1305 Size: 1677 2886 184 2007 Fragments arranged by size: 2886 2007 1677 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4932 Fragments arranged by size: 4932 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4712 5071 5230 518 Size: 648 3546 359 159 2042 Fragments arranged by size: 3546 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2066 2596 3701 3865 3880 3886 4170 4847 Size: 460 530 1105 164 15 6 284 677 Cuts at: 4847 5995 6240 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1105 677 530 460 284 245 164 15 6 NcoI C'CATG_G Cuts at: 513 1065 2541 3150 3804 513 Size: 552 1476 609 654 3463 Fragments arranged by size: 3463 1476 654 609 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6754 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6754 NotI GC'GGCC_GC Cuts at: 4201 4201 Size: 6754 NspI r_CATG'y Cuts at: 1132 1229 4112 5657 1132 Size: 97 2883 1545 2229 Fragments arranged by size: 2883 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6754 PciI A'CATG_T Cuts at: 5653 5653 Size: 6754 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6754 PmeI GTTT'AAAC Cuts at: 4058 4058 Size: 6754 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4823 4855 6336 6368 4823 Size: 32 1481 32 5209 Fragments arranged by size: 5209 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 3995 2143 Size: 1852 4902 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6754 PsiI TTA'TAA Cuts at: 2502 4324 2502 Size: 1822 4932 PspOMI G'GGCC_C Cuts at: 3947 3947 Size: 6754 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3704 3736 3704 Size: 32 6722 PstI C_TGCA'G Cuts at: 830 2391 3696 3885 4106 830 Size: 1561 1305 189 221 3478 Fragments arranged by size: 3478 1561 1305 221 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6754 PvuII CAG'CTG Cuts at: 2066 3865 3880 2066 Size: 1799 15 4940 Fragments arranged by size: 4940 1799 15 SalI G'TCGA_C Cuts at: 1936 4096 1936 Size: 2160 4594 SanDI GG'GwC_CC Cuts at: 2143 2143 Size: 6754 SapI GCTCTTCn'nnn_ Cuts at: 2216 3992 2216 Size: 1776 4978 SbfI CC_TGCA'GG Cuts at: 4106 4106 Size: 6754 ScaI AGT'ACT Cuts at: 5002 6714 5002 Size: 1712 5042 SfcI C'TryA_G Cuts at: 826 1045 2113 2189 2387 2423 3692 3881 4102 Size: 219 1068 76 198 36 1269 189 221 Cuts at: 4102 5237 5918 6109 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1269 1135 1068 681 221 219 198 191 189 76 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6754 SmaI CCC'GGG Cuts at: 1872 4081 1872 Size: 2209 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2651 3308 4858 5759 6021 820 Size: 197 926 708 657 1550 901 262 1553 Fragments arranged by size: 1553 1550 926 901 708 657 262 197 SnaBI TAC'GTA Cuts at: 493 4545 493 Size: 4052 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6754 SphI G_CATG'C Cuts at: 1132 4112 1132 Size: 2980 3774 SspI AAT'ATT Cuts at: 5 52 4678 5 Size: 47 4626 2081 Fragments arranged by size: 4626 2081 47 StuI AGG'CCT Cuts at: 1517 2846 1517 Size: 1329 5425 StyI C'CwwG_G Cuts at: 513 1065 2541 2895 3150 3543 3804 3848 3951 Size: 552 1476 354 255 393 261 44 103 Cuts at: 3951 4193 513 Size: 242 3074 Fragments arranged by size: 3074 1476 552 393 354 261 255 242 103 44 SwaI ATTT'AAAT Cuts at: 4018 4018 Size: 6754 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3051 3322 3335 3559 3935 4781 4798 Size: 1255 1471 271 13 224 376 846 17 Cuts at: 4798 4951 5136 5581 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1471 1255 846 445 376 271 224 185 153 17 13 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 3304 5000 Size: 274 80 33 51 1443 195 1132 1696 Cuts at: 5000 6712 96 Size: 1712 138 Fragments arranged by size: 1712 1696 1443 1132 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3726 3758 3959 3991 6714 6746 3726 Size: 32 201 32 2723 32 3734 Fragments arranged by size: 3734 2723 201 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3200 1316 Size: 1884 4870 XbaI T'CTAG_A Cuts at: 4090 4090 Size: 6754 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3619 3619 Size: 6754 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6754 XmaI C'CCGG_G Cuts at: 1870 4079 1870 Size: 2209 4545 XmnI GAAnn'nnTTC Cuts at: 4883 4883 Size: 6754 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4562 276 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SexAI SfiI SfoI SrfI