(Circular) (Six-base) MAPSORT of: pFN21AA0972.seq Check: 3104 from: 1 to: 6754 pFN21AA0972 6754 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2937 2937 Size: 6754 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3170 4564 278 Size: 53 83 186 936 1634 1394 2468 Fragments arranged by size: 2468 1634 1394 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4007 4097 1937 Size: 2070 90 4594 Fragments arranged by size: 4594 2070 90 Acc65I G'GTAC_C Cuts at: 4075 4075 Size: 6754 AclI AA'CG_TT Cuts at: 1563 4881 5254 1563 Size: 3318 373 3063 Fragments arranged by size: 3318 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4817 6201 6469 1632 Size: 3185 1384 268 1917 Fragments arranged by size: 3185 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6557 AflIII A'CryG_T Cuts at: 1203 5653 6630 1203 Size: 4450 977 1327 Fragments arranged by size: 4450 1327 977 AgeI A'CCGG_T Cuts at: 5585 5585 Size: 6754 AhdI GACnn_n'nnGTC Cuts at: 5483 5483 Size: 6754 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6754 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2514 1184 Size: 1330 5424 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2303 2335 2303 Size: 32 6722 AlwNI CAG_nnn'CTG Cuts at: 4497 6069 4497 Size: 1572 5182 ApaLI G'TGCA_C Cuts at: 4811 5967 4811 Size: 1156 5598 ApoI r'AATT_y Cuts at: 1493 2165 2333 2548 2567 2629 2675 2861 3360 Size: 672 168 215 19 62 46 186 499 Cuts at: 3360 3444 3528 3780 3827 3864 4029 4063 4289 Size: 84 84 252 47 37 165 34 226 Cuts at: 4289 4441 6550 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 672 499 252 226 215 186 168 165 152 84 84 62 47 46 37 34 19 AseI AT'TA_AT Cuts at: 160 1032 2577 5308 160 Size: 872 1545 2731 1606 Fragments arranged by size: 2731 1606 1545 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 AvaI C'yCGr_G Cuts at: 1870 1943 4079 1870 Size: 73 2136 4545 Fragments arranged by size: 4545 2136 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2254 2287 2254 Size: 33 6721 BamHI G'GATC_C Cuts at: 4084 4451 4084 Size: 367 6387 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4075 5530 618 Size: 325 217 627 2288 1455 1842 Fragments arranged by size: 2288 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4073 1391 Size: 2682 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5783 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4945 4979 1903 Size: 34 3008 34 3678 Fragments arranged by size: 3678 3008 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4646 5856 4646 Size: 1210 5544 BclI T'GATC_A Cuts at: 1548 2379 3853 1548 Size: 831 1474 4449 Fragments arranged by size: 4449 1474 831 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5365 136 Size: 107 122 71 631 605 3693 1525 Fragments arranged by size: 3693 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6749 6749 Size: 6754 BlpI GC'TnA_GC Cuts at: 4171 4171 Size: 6754 Bme1580I G_kGCm'C Cuts at: 1790 4815 5971 1790 Size: 3025 1156 2573 Fragments arranged by size: 3025 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2155 1318 Size: 837 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3630 3798 4485 Size: 416 53 83 399 24 2213 168 687 Cuts at: 4485 5443 442 Size: 958 1753 Fragments arranged by size: 2213 1753 958 687 416 399 168 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6754 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2353 2353 Size: 6754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2688 5414 1239 Size: 1013 436 2726 2579 Fragments arranged by size: 2726 2579 1013 436 Bpu10I CC'TnA_GC Cuts at: 4013 4013 Size: 6754 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4879 5744 6042 4879 Size: 865 298 5591 Fragments arranged by size: 5591 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5417 882 Size: 617 3918 2219 Fragments arranged by size: 3918 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4545 493 Size: 711 3341 2702 Fragments arranged by size: 3341 2702 711 BsaBI GATnn'nnATC Cuts at: 6748 6748 Size: 6754 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3167 4561 4943 5578 Size: 53 83 186 936 1634 1394 382 635 Cuts at: 5578 275 Size: 1451 Fragments arranged by size: 1634 1451 1394 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3834 5187 5585 5859 6006 6417 3834 Size: 1353 398 274 147 411 4171 Fragments arranged by size: 4171 1353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2303 2333 1178 Size: 30 165 30 900 30 5599 Fragments arranged by size: 5599 900 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2324 2342 1186 Size: 1006 132 18 5598 Fragments arranged by size: 5598 1006 132 18 BseYI C'CCAG_C Cuts at: 5957 6453 5957 Size: 496 6258 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6181 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4204 4965 5993 1540 Size: 275 186 2203 761 1028 2301 Fragments arranged by size: 2301 2203 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4815 4900 5971 4815 Size: 85 1071 5598 Fragments arranged by size: 5598 1071 85 BsmI GAATG_Cn' Cuts at: 3980 4265 4358 3980 Size: 285 93 6376 Fragments arranged by size: 6376 285 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2257 2257 Size: 6754 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4073 4815 4900 5971 1391 Size: 399 2283 742 85 1071 2174 Fragments arranged by size: 2283 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 3834 6417 3834 Size: 2583 4171 BspHI T'CATG_A Cuts at: 3142 4643 6373 3142 Size: 1501 1730 3523 Fragments arranged by size: 3523 1730 1501 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2937 4109 844 Size: 2093 1172 3489 Fragments arranged by size: 3489 2093 1172 BsrBI CCG'CTC Cuts at: 1394 1823 4641 1394 Size: 429 2818 3507 Fragments arranged by size: 3507 2818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3985 5249 5423 65 Size: 1183 2737 1264 174 1396 Fragments arranged by size: 2737 1396 1264 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5398 5585 1700 Size: 110 3588 187 2869 Fragments arranged by size: 3588 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2214 96 Size: 2118 4636 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6754 BssSI C'ACGA_G Cuts at: 4814 5826 4814 Size: 1012 5742 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6754 BstBI TT'CG_AA Cuts at: 5558 5558 Size: 6754 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6754 BstYI r'GATC_y Cuts at: 1972 4084 4451 4836 4853 6305 6391 6403 6749 Size: 2112 367 385 17 1452 86 12 346 Cuts at: 6749 1972 Size: 1977 Fragments arranged by size: 2112 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3428 3428 Size: 6754 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6202 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6015 Fragments arranged by size: 6015 683 56 BtsI GCAGTG_nn' Cuts at: 4270 5064 5084 4270 Size: 794 20 5940 Fragments arranged by size: 5940 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6754 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3547 3582 529 Size: 35 2983 35 3701 Fragments arranged by size: 3701 2983 35 35 DraI TTT'AAA Cuts at: 3805 4058 3805 Size: 253 6501 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5761 809 Size: 507 4445 1802 Fragments arranged by size: 4445 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3580 3916 4201 Size: 54 1425 50 275 18 1750 336 285 Cuts at: 4201 5090 6719 8 Size: 889 1629 43 Fragments arranged by size: 1750 1629 1425 889 336 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4201 1537 Size: 275 2389 4090 Fragments arranged by size: 4090 2389 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 3078 4684 1353 Size: 1101 624 1606 3423 Fragments arranged by size: 3423 1606 1101 624 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5341 5715 5861 387 Size: 1064 138 3752 374 146 1280 Fragments arranged by size: 3752 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2688 4817 5414 6201 6469 1239 Size: 393 620 436 2129 597 787 268 1524 Fragments arranged by size: 2129 1524 787 620 597 436 393 268 EcoRI G'AATT_C Cuts at: 2165 2333 2629 3360 3444 3528 3780 3864 4063 Size: 168 296 731 84 84 252 84 199 Cuts at: 4063 4441 2165 Size: 378 4478 Fragments arranged by size: 4478 731 378 296 252 199 168 84 84 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3863 3863 Size: 6754 FspI TGC'GCA Cuts at: 5260 5260 Size: 6754 HaeII r_GCGC'y Cuts at: 1678 5901 1678 Size: 4223 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5395 5427 5469 5501 1373 Size: 32 135 32 3823 32 42 32 2626 Fragments arranged by size: 3823 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2967 3423 4098 4344 669 Size: 988 281 1029 456 675 246 3079 Fragments arranged by size: 3079 1029 988 675 456 281 246 HindIII A'AGCT_T Cuts at: 748 2760 4015 748 Size: 2012 1255 3487 Fragments arranged by size: 3487 2012 1255 HpaI GTT'AAC Cuts at: 4344 4344 Size: 6754 KpnI G_GTAC'C Cuts at: 4079 4079 Size: 6754 MfeI C'AATT_G Cuts at: 4353 4353 Size: 6754 MluI A'CGCG_T Cuts at: 6630 6630 Size: 6754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2277 5868 6052 1305 Size: 972 3591 184 2007 Fragments arranged by size: 3591 2007 972 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3582 3918 10 Size: 54 1425 343 1750 336 2846 Fragments arranged by size: 2846 1750 1425 343 336 54 MslI CAynn'nnrTG Cuts at: 518 1166 3301 3721 3805 4712 5071 5230 518 Size: 648 2135 420 84 907 359 159 2042 Fragments arranged by size: 2135 2042 907 648 420 359 159 84 MspA1I CmG'CkG Cuts at: 1606 3074 4170 4847 5995 6240 1606 Size: 1468 1096 677 1148 245 2120 Fragments arranged by size: 2120 1468 1148 1096 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6202 NdeI CA'TA_TG Cuts at: 387 387 Size: 6754 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6754 NotI GC'GGCC_GC Cuts at: 4201 4201 Size: 6754 NspI r_CATG'y Cuts at: 1132 1229 2495 4112 5657 1132 Size: 97 1266 1617 1545 2229 Fragments arranged by size: 2229 1617 1545 1266 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6754 PciI A'CATG_T Cuts at: 5653 5653 Size: 6754 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6754 PmeI GTTT'AAAC Cuts at: 4058 4058 Size: 6754 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2303 2335 4823 4855 6336 6368 2303 Size: 32 2488 32 1481 32 2689 Fragments arranged by size: 2689 2488 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6754 PsiI TTA'TAA Cuts at: 2715 4324 2715 Size: 1609 5145 PstI C_TGCA'G Cuts at: 830 4106 830 Size: 3276 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6754 PvuII CAG'CTG Cuts at: 3074 3074 Size: 6754 SalI G'TCGA_C Cuts at: 1936 4096 1936 Size: 2160 4594 SbfI CC_TGCA'GG Cuts at: 4106 4106 Size: 6754 ScaI AGT'ACT Cuts at: 3349 5002 6714 3349 Size: 1653 1712 3389 Fragments arranged by size: 3389 1712 1653 SfcI C'TryA_G Cuts at: 826 1045 2160 2239 2451 4102 5237 5918 6109 Size: 219 1115 79 212 1651 1135 681 191 Cuts at: 6109 826 Size: 1471 Fragments arranged by size: 1651 1471 1135 1115 681 219 212 191 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6754 SmaI CCC'GGG Cuts at: 1872 4081 1872 Size: 2209 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4858 5759 6021 820 Size: 197 926 2915 901 262 1553 Fragments arranged by size: 2915 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4545 493 Size: 4052 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6754 SphI G_CATG'C Cuts at: 1132 4112 1132 Size: 2980 3774 SspI AAT'ATT Cuts at: 5 52 4678 5 Size: 47 4626 2081 Fragments arranged by size: 4626 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6754 StyI C'CwwG_G Cuts at: 513 1065 2315 4193 513 Size: 552 1250 1878 3074 Fragments arranged by size: 3074 1878 1250 552 SwaI ATTT'AAAT Cuts at: 3805 3805 Size: 6754 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2186 2367 4781 4798 4951 5136 5581 Size: 1255 606 181 2414 17 153 185 445 Cuts at: 5581 325 Size: 1498 Fragments arranged by size: 2414 1498 1255 606 445 185 181 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 3347 5000 Size: 274 80 33 51 1443 237 1133 1653 Cuts at: 5000 6712 96 Size: 1712 138 Fragments arranged by size: 1712 1653 1443 1133 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2860 2892 3940 3972 6714 6746 2860 Size: 32 1048 32 2742 32 2868 Fragments arranged by size: 2868 2742 1048 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6754 XbaI T'CTAG_A Cuts at: 4090 4090 Size: 6754 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2906 2906 Size: 6754 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6754 XmaI C'CCGG_G Cuts at: 1870 4079 1870 Size: 2209 4545 XmnI GAAnn'nnTTC Cuts at: 2590 2724 3239 3716 4883 2590 Size: 134 515 477 1167 4461 Fragments arranged by size: 4461 1167 515 477 134 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3168 4562 276 Size: 53 83 186 936 1634 1394 2468 Fragments arranged by size: 2468 1634 1394 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI