(Circular) (Six-base) MAPSORT of: pFN21AA0950.seq Check: 9445 from: 1 to: 5653 pFN21AA0950 5653 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2053 2053 Size: 5653 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2322 2996 1937 Size: 385 674 4594 Fragments arranged by size: 4594 674 385 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2623 2695 3716 5100 5368 1632 Size: 991 72 1021 1384 268 1917 Fragments arranged by size: 1917 1384 1021 991 268 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 2177 2585 1166 Size: 1011 408 4234 Fragments arranged by size: 4234 1011 408 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 2347 2830 3396 4968 2347 Size: 483 566 1572 3032 Fragments arranged by size: 3032 1572 566 483 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2962 3188 3340 5449 1493 Size: 1469 226 152 2109 1697 Fragments arranged by size: 2109 1697 1469 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2978 1870 Size: 73 1035 4545 Fragments arranged by size: 4545 1035 73 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 2974 4429 618 Size: 325 217 627 392 795 1455 1842 Fragments arranged by size: 1842 1455 795 627 392 325 217 BanII G_rGCy'C Cuts at: 1391 2265 2861 2884 2972 1391 Size: 874 596 23 88 4072 Fragments arranged by size: 4072 874 596 88 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2629 928 Size: 971 730 3952 Fragments arranged by size: 3952 971 730 BbvCI CC'TCA_GC Cuts at: 2101 2101 Size: 5653 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4264 136 Size: 107 122 71 631 605 2592 1525 Fragments arranged by size: 2592 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 2855 3070 2855 Size: 215 5438 Bme1580I G_kGCm'C Cuts at: 1790 2100 2757 3714 4870 1790 Size: 310 657 957 1156 2573 Fragments arranged by size: 2573 1156 957 657 310 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2437 2562 2792 Size: 416 53 83 399 24 1020 125 230 Cuts at: 2792 2818 3384 4342 442 Size: 26 566 958 1753 Fragments arranged by size: 1753 1020 958 566 416 399 230 125 83 53 26 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2073 2209 4313 1239 Size: 834 136 2104 2579 Fragments arranged by size: 2579 2104 834 136 Bpu10I CC'TnA_GC Cuts at: 2101 2101 Size: 5653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2247 4316 882 Size: 617 748 2069 2219 Fragments arranged by size: 2219 2069 748 617 BsaAI yAC'GTr Cuts at: 493 1204 3444 493 Size: 711 2240 2702 Fragments arranged by size: 2702 2240 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2845 3460 3842 4477 Size: 53 83 186 936 1312 615 382 635 Cuts at: 4477 275 Size: 1451 Fragments arranged by size: 1451 1312 936 635 615 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2249 4086 4484 4758 4905 5316 2249 Size: 1837 398 274 147 411 2586 Fragments arranged by size: 2586 1837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2567 2597 2651 2681 2778 Size: 30 165 30 1164 30 54 30 97 Cuts at: 2778 2808 1178 Size: 30 4023 Fragments arranged by size: 4023 1164 165 97 54 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2879 1186 Size: 1693 3960 BseYI C'CCAG_C Cuts at: 2164 2196 2213 2280 4856 5352 2164 Size: 32 17 67 2576 496 2465 Fragments arranged by size: 2576 2465 496 67 32 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5080 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2265 2685 3714 3799 4870 2265 Size: 420 1029 85 1071 3048 Fragments arranged by size: 3048 1071 1029 420 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2265 2685 2757 2861 2884 2972 Size: 399 310 165 420 72 104 23 88 Cuts at: 2972 3714 3799 4870 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 420 399 310 165 104 88 85 72 23 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 2697 3542 5272 2697 Size: 845 1730 3078 Fragments arranged by size: 3078 1730 845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 3008 844 Size: 1209 955 3489 Fragments arranged by size: 3489 1209 955 BsrBI CCG'CTC Cuts at: 1394 1823 2789 3540 1394 Size: 429 966 751 3507 Fragments arranged by size: 3507 966 751 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 4322 65 Size: 1183 2900 174 1396 Fragments arranged by size: 2900 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 2065 2438 2774 794 Size: 1271 373 336 3673 Fragments arranged by size: 3673 1271 373 336 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 2350 2837 1184 Size: 1166 487 4000 Fragments arranged by size: 4000 1166 487 BstYI r'GATC_y Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648 Size: 1011 367 385 17 1452 86 12 346 Cuts at: 5648 1972 Size: 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2862 2862 Size: 5653 BtgI C'CryG_G Cuts at: 513 1065 2025 2538 513 Size: 552 960 513 3628 Fragments arranged by size: 3628 960 552 513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 2100 3169 3963 3983 2100 Size: 1069 794 20 3770 Fragments arranged by size: 3770 1069 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2727 3100 3989 Size: 54 1425 50 275 18 897 373 889 Cuts at: 3989 5618 8 Size: 1629 43 Fragments arranged by size: 1629 1425 897 889 373 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2270 2690 3583 1353 Size: 917 420 893 3423 Fragments arranged by size: 3423 917 893 420 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2263 2263 Size: 5653 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2209 2623 2695 3716 4313 5100 Size: 393 441 136 414 72 1021 597 787 Cuts at: 5100 5368 1239 Size: 268 1524 Fragments arranged by size: 1524 1021 787 597 441 414 393 268 136 72 EcoO109I rG'GnC_Cy Cuts at: 2038 2154 2038 Size: 116 5537 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2677 2677 Size: 5653 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 4800 1678 Size: 3122 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2295 2327 2651 2683 4294 Size: 32 135 32 723 32 324 32 1611 Cuts at: 4294 4326 4368 4400 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1611 723 324 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4767 4951 1305 Size: 3462 184 2007 Fragments arranged by size: 3462 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2729 10 Size: 54 1425 343 897 2934 Fragments arranged by size: 2934 1425 897 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2555 2585 3611 3970 4129 518 Size: 648 1011 378 30 1026 359 159 2042 Fragments arranged by size: 2042 1026 1011 648 378 359 159 30 MspA1I CmG'CkG Cuts at: 1606 2177 2280 2344 2715 3069 3746 4894 5139 Size: 571 103 64 371 354 677 1148 245 Cuts at: 5139 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 571 371 354 245 103 64 NcoI C'CATG_G Cuts at: 513 1065 2538 513 Size: 552 1473 3628 Fragments arranged by size: 3628 1473 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 2011 2042 1348 Size: 663 31 4959 Fragments arranged by size: 4959 663 31 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PstI C_TGCA'G Cuts at: 830 2931 3005 830 Size: 2101 74 3478 Fragments arranged by size: 3478 2101 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2280 2344 2280 Size: 64 5589 SacI G_AGCT'C Cuts at: 2265 2265 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SapI GCTCTTCn'nnn_ Cuts at: 2270 2690 2270 Size: 420 5233 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2927 3001 4136 4817 5008 826 Size: 219 1882 74 1135 681 191 1471 Fragments arranged by size: 1882 1471 1135 681 219 191 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3757 4658 4920 820 Size: 197 926 1814 901 262 1553 Fragments arranged by size: 1814 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2355 2538 3092 513 Size: 552 1290 183 554 3074 Fragments arranged by size: 3074 1290 554 552 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2063 2837 3680 3697 3850 4035 4480 Size: 1255 483 774 843 17 153 185 445 Cuts at: 4480 325 Size: 1498 Fragments arranged by size: 1498 1255 843 774 483 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3899 5611 96 Size: 274 80 33 51 1443 1922 1712 138 Fragments arranged by size: 1922 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5613 5645 5613 Size: 32 5621 Tth111I GACn'n_nGTC Cuts at: 1316 2390 2402 1316 Size: 1074 12 4567 Fragments arranged by size: 4567 1074 12 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2424 2424 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI