(Circular) (Six-base) MAPSORT of: pFN21AA0942.seq Check: 680 from: 1 to: 6244 pFN21AA0942 6244 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3291 4054 278 Size: 53 83 186 936 1755 763 2468 Fragments arranged by size: 2468 1755 936 763 186 83 53 AccI GT'mk_AC Cuts at: 1937 3587 1937 Size: 1650 4594 Acc65I G'GTAC_C Cuts at: 2132 3565 2132 Size: 1433 4811 AclI AA'CG_TT Cuts at: 1563 4371 4744 1563 Size: 2808 373 3063 Fragments arranged by size: 3063 2808 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2288 2876 2934 3218 3239 3437 4307 5691 Size: 656 588 58 284 21 198 870 1384 Cuts at: 5691 5959 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 870 656 588 284 268 198 58 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6047 AflIII A'CryG_T Cuts at: 1203 2991 3486 5143 6120 1203 Size: 1788 495 1657 977 1327 Fragments arranged by size: 1788 1657 1327 977 495 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 2694 4973 2694 Size: 2279 3965 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6244 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6244 AlwNI CAG_nnn'CTG Cuts at: 3987 5559 3987 Size: 1572 4672 ApaLI G'TGCA_C Cuts at: 2942 4301 5457 2942 Size: 1359 1156 3729 Fragments arranged by size: 3729 1359 1156 ApoI r'AATT_y Cuts at: 1493 2094 2237 2784 3553 3779 3931 6040 1493 Size: 601 143 547 769 226 152 2109 1697 Fragments arranged by size: 2109 1697 769 601 547 226 152 143 AseI AT'TA_AT Cuts at: 160 1032 4798 160 Size: 872 3766 1606 Fragments arranged by size: 3766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 3430 3569 1870 Size: 73 1487 139 4545 Fragments arranged by size: 4545 1487 139 73 BamHI G'GATC_C Cuts at: 3574 3941 3574 Size: 367 5877 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 3565 5020 618 Size: 325 217 627 345 1433 1455 1842 Fragments arranged by size: 1842 1455 1433 627 345 325 217 BanII G_rGCy'C Cuts at: 1391 2012 3047 3563 1391 Size: 621 1035 516 4072 Fragments arranged by size: 4072 1035 621 516 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2816 928 Size: 971 917 4356 Fragments arranged by size: 4356 971 917 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4435 4469 1903 Size: 34 2498 34 3678 Fragments arranged by size: 3678 2498 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3445 4136 5346 3445 Size: 691 1210 4343 Fragments arranged by size: 4343 1210 691 BclI T'GATC_A Cuts at: 1548 1548 Size: 6244 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4855 136 Size: 107 122 71 631 605 3183 1525 Fragments arranged by size: 3183 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6239 6239 Size: 6244 BlpI GC'TnA_GC Cuts at: 3661 3661 Size: 6244 Bme1580I G_kGCm'C Cuts at: 1790 2946 4305 5461 1790 Size: 1156 1359 1156 2573 Fragments arranged by size: 2573 1359 1156 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6244 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3975 4933 442 Size: 416 53 83 399 24 2558 958 1753 Fragments arranged by size: 2558 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3268 3268 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3037 3341 4904 1239 Size: 1798 304 1563 2579 Fragments arranged by size: 2579 1798 1563 304 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2334 3347 4369 5234 5532 2334 Size: 1013 1022 865 298 3046 Fragments arranged by size: 3046 1022 1013 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2102 4907 882 Size: 617 603 2805 2219 Fragments arranged by size: 2805 2219 617 603 BsaAI yAC'GTr Cuts at: 493 1204 4035 493 Size: 711 2831 2702 Fragments arranged by size: 2831 2702 711 BsaBI GATnn'nnATC Cuts at: 6238 6238 Size: 6244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3288 4051 4433 5068 Size: 53 83 186 936 1755 763 382 635 Cuts at: 5068 275 Size: 1451 Fragments arranged by size: 1755 1451 936 763 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4677 5075 5349 5496 5907 4677 Size: 398 274 147 411 5014 Fragments arranged by size: 5014 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6019 Fragments arranged by size: 6019 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2675 3203 1186 Size: 1489 528 4227 Fragments arranged by size: 4227 1489 528 BseYI C'CCAG_C Cuts at: 2042 5447 5943 2042 Size: 3405 496 2343 Fragments arranged by size: 3405 2343 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3087 1163 Size: 573 1351 4320 Fragments arranged by size: 4320 1351 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3694 4455 5483 1540 Size: 275 186 1693 761 1028 2301 Fragments arranged by size: 2301 1693 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2090 2946 3249 4305 4390 5461 2090 Size: 856 303 1056 85 1071 2873 Fragments arranged by size: 2873 1071 1056 856 303 85 BsmI GAATG_Cn' Cuts at: 2327 3357 3755 3848 2327 Size: 1030 398 93 4723 Fragments arranged by size: 4723 1030 398 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2090 2946 3047 3249 3563 4305 Size: 399 222 78 856 101 202 314 742 Cuts at: 4305 4390 5461 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 856 742 399 314 222 202 101 85 78 BspEI T'CCGG_A Cuts at: 5907 5907 Size: 6244 BspHI T'CATG_A Cuts at: 2737 4133 5863 2737 Size: 1396 1730 3118 Fragments arranged by size: 3118 1730 1396 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3599 844 Size: 2755 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3252 4131 1394 Size: 429 1429 879 3507 Fragments arranged by size: 3507 1429 879 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2044 2149 2412 2434 2517 4739 4913 Size: 1183 796 105 263 22 83 2222 174 Cuts at: 4913 65 Size: 1396 Fragments arranged by size: 2222 1396 1183 796 263 174 105 83 22 BsrFI r'CCGG_y Cuts at: 1700 1810 4888 5075 1700 Size: 110 3078 187 2869 Fragments arranged by size: 3078 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2580 2757 96 Size: 2484 177 3583 Fragments arranged by size: 3583 2484 177 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6244 BssSI C'ACGA_G Cuts at: 4304 5316 4304 Size: 1012 5232 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6244 BstBI TT'CG_AA Cuts at: 5048 5048 Size: 6244 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6244 BstXI CCAn_nnnn'nTGG Cuts at: 2164 2489 3238 2164 Size: 325 749 5170 Fragments arranged by size: 5170 749 325 BstYI r'GATC_y Cuts at: 1972 2563 3574 3941 4326 4343 5795 5881 5893 Size: 591 1011 367 385 17 1452 86 12 Cuts at: 5893 6239 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1011 591 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2967 513 Size: 552 939 963 3790 Fragments arranged by size: 3790 963 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5505 Fragments arranged by size: 5505 683 56 BtsI GCAGTG_nn' Cuts at: 3465 3760 4554 4574 3465 Size: 295 794 20 5135 Fragments arranged by size: 5135 794 295 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6244 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 2323 3548 2323 Size: 1225 5019 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3020 3272 5251 809 Size: 507 1704 252 1979 1802 Fragments arranged by size: 1979 1802 1704 507 252 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2479 3240 Size: 54 1425 50 275 18 336 313 761 Cuts at: 3240 3691 4580 6209 8 Size: 451 889 1629 43 Fragments arranged by size: 1629 1425 889 761 451 336 313 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3691 1537 Size: 275 1879 4090 Fragments arranged by size: 4090 1879 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2017 2622 2910 4174 1353 Size: 664 605 288 1264 3423 Fragments arranged by size: 3423 1264 664 605 288 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4831 5205 5351 387 Size: 1064 138 3242 374 146 1280 Fragments arranged by size: 3242 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2876 2934 3037 3218 3239 3341 Size: 393 656 588 58 103 181 21 102 Cuts at: 3341 3437 4307 4904 5691 5959 1239 Size: 96 870 597 787 268 1524 Fragments arranged by size: 1524 870 787 656 597 588 393 268 181 103 102 96 58 21 EcoRI G'AATT_C Cuts at: 3553 3931 3553 Size: 378 5866 FspI TGC'GCA Cuts at: 4750 4750 Size: 6244 HaeII r_GCGC'y Cuts at: 1678 5391 1678 Size: 3713 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2284 2316 2491 2523 2680 Size: 32 135 32 712 32 175 32 157 Cuts at: 2680 2712 3487 3519 4885 4917 4959 4991 1373 Size: 32 775 32 1366 32 42 32 2626 Fragments arranged by size: 2626 1366 775 712 175 157 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3588 3834 669 Size: 988 281 1650 246 3079 Fragments arranged by size: 3079 1650 988 281 246 HindIII A'AGCT_T Cuts at: 748 2603 748 Size: 1855 4389 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KpnI G_GTAC'C Cuts at: 2136 3569 2136 Size: 1433 4811 MfeI C'AATT_G Cuts at: 3079 3843 3079 Size: 764 5480 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5358 5542 1305 Size: 4053 184 2007 Fragments arranged by size: 4053 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2168 2481 10 Size: 54 1425 343 336 313 3773 Fragments arranged by size: 3773 1425 343 336 313 54 MslI CAynn'nnrTG Cuts at: 518 1166 4202 4561 4720 518 Size: 648 3036 359 159 2042 Fragments arranged by size: 3036 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2185 3092 3660 4337 5485 5730 1606 Size: 579 907 568 677 1148 245 2120 Fragments arranged by size: 2120 1148 907 677 579 568 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4753 Fragments arranged by size: 4753 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 3691 3691 Size: 6244 NspI r_CATG'y Cuts at: 1132 1229 3602 5147 1132 Size: 97 2373 1545 2229 Fragments arranged by size: 2373 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6244 PciI A'CATG_T Cuts at: 5143 5143 Size: 6244 PflMI CCAn_nnn'nTGG Cuts at: 1108 2953 3319 1108 Size: 1845 366 4033 Fragments arranged by size: 4033 1845 366 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4313 4345 5826 5858 4313 Size: 32 1481 32 4699 Fragments arranged by size: 4699 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6244 PsiI TTA'TAA Cuts at: 3814 3814 Size: 6244 PspXI vC'TCGA_Gb Cuts at: 3430 3430 Size: 6244 PstI C_TGCA'G Cuts at: 830 3412 3596 830 Size: 2582 184 3478 Fragments arranged by size: 3478 2582 184 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6244 PvuII CAG'CTG Cuts at: 2185 3092 2185 Size: 907 5337 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SapI GCTCTTCn'nnn_ Cuts at: 2017 2017 Size: 6244 SbfI CC_TGCA'GG Cuts at: 3596 3596 Size: 6244 ScaI AGT'ACT Cuts at: 2656 2710 4492 6204 2656 Size: 54 1782 1712 2696 Fragments arranged by size: 2696 1782 1712 54 SfcI C'TryA_G Cuts at: 826 1045 3408 3592 4727 5408 5599 826 Size: 219 2363 184 1135 681 191 1471 Fragments arranged by size: 2363 1471 1135 681 219 191 184 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 3571 1872 Size: 1699 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2349 3362 3430 4348 5249 5511 Size: 197 926 406 1013 68 918 901 262 Cuts at: 5511 820 Size: 1553 Fragments arranged by size: 1553 1013 926 918 901 406 262 197 68 SnaBI TAC'GTA Cuts at: 493 4035 493 Size: 3542 2702 SpeI A'CTAG_T Cuts at: 152 2248 152 Size: 2096 4148 SphI G_CATG'C Cuts at: 1132 3602 1132 Size: 2470 3774 SspI AAT'ATT Cuts at: 5 52 2488 4168 5 Size: 47 2436 1680 2081 Fragments arranged by size: 2436 2081 1680 47 StuI AGG'CCT Cuts at: 1517 2906 1517 Size: 1389 4855 StyI C'CwwG_G Cuts at: 513 1065 2004 2221 2559 2961 3282 3683 513 Size: 552 939 217 338 402 321 401 3074 Fragments arranged by size: 3074 939 552 402 401 338 321 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4271 4288 4441 4626 5071 325 Size: 1255 2691 17 153 185 445 1498 Fragments arranged by size: 2691 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2580 2654 2708 Size: 274 80 33 51 1443 603 74 54 Cuts at: 2708 2757 2851 3297 4490 6202 96 Size: 49 94 446 1193 1712 138 Fragments arranged by size: 1712 1443 1193 603 446 274 138 94 80 74 54 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6204 6236 6204 Size: 32 6212 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6244 XbaI T'CTAG_A Cuts at: 3580 3580 Size: 6244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2143 2143 Size: 6244 XhoI C'TCGA_G Cuts at: 1943 3430 1943 Size: 1487 4757 XmaI C'CCGG_G Cuts at: 1870 3569 1870 Size: 1699 4545 XmnI GAAnn'nnTTC Cuts at: 4373 4373 Size: 6244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3289 4052 276 Size: 53 83 186 936 1755 763 2468 Fragments arranged by size: 2468 1755 936 763 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI