(Circular) (Six-base) MAPSORT of: pFN21AA0904.seq Check: 7837 from: 1 to: 9148 pFN21AA0904 9148 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5931 5931 Size: 9148 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5152 6958 278 Size: 53 83 186 936 3616 1806 2468 Fragments arranged by size: 3616 2468 1806 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4948 6491 1937 Size: 3011 1543 4594 Fragments arranged by size: 4594 3011 1543 Acc65I G'GTAC_C Cuts at: 4704 6469 4704 Size: 1765 7383 AclI AA'CG_TT Cuts at: 1563 3881 4738 7275 7648 1563 Size: 2318 857 2537 373 3063 Fragments arranged by size: 3063 2537 2318 857 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2594 3414 4289 4683 5559 5922 6260 7211 Size: 962 820 875 394 876 363 338 951 Cuts at: 7211 8595 8863 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 962 951 876 875 820 394 363 338 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8951 AflIII A'CryG_T Cuts at: 1203 3989 8047 9024 1203 Size: 2786 4058 977 1327 Fragments arranged by size: 4058 2786 1327 977 AgeI A'CCGG_T Cuts at: 7979 7979 Size: 9148 AhdI GACnn_n'nnGTC Cuts at: 7877 7877 Size: 9148 AleI CACnn'nnGTG Cuts at: 1166 4356 1166 Size: 3190 5958 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9148 AlwNI CAG_nnn'CTG Cuts at: 3230 3383 5452 6085 6891 8463 3230 Size: 153 2069 633 806 1572 3915 Fragments arranged by size: 3915 2069 1572 806 633 153 ApaI G_GGCC'C Cuts at: 5727 6362 5727 Size: 635 8513 ApaLI G'TGCA_C Cuts at: 4490 5029 7205 8361 4490 Size: 539 2176 1156 5277 Fragments arranged by size: 5277 2176 1156 539 ApoI r'AATT_y Cuts at: 1493 3424 3802 4113 4564 4958 6457 6683 6835 Size: 1931 378 311 451 394 1499 226 152 Cuts at: 6835 8944 1493 Size: 2109 1697 Fragments arranged by size: 2109 1931 1697 1499 451 394 378 311 226 152 AseI AT'TA_AT Cuts at: 160 1032 7702 160 Size: 872 6670 1606 Fragments arranged by size: 6670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9148 AvaI C'yCGr_G Cuts at: 1870 1943 2361 2716 2770 2812 6473 1870 Size: 73 418 355 54 42 3661 4545 Fragments arranged by size: 4545 3661 418 355 73 54 42 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3825 3858 3825 Size: 33 9115 BamHI G'GATC_C Cuts at: 6478 6845 6478 Size: 367 8781 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4424 4704 6469 7924 618 Size: 325 217 627 2637 280 1765 1455 1842 Fragments arranged by size: 2637 1842 1765 1455 627 325 280 217 BanII G_rGCy'C Cuts at: 1391 2672 2885 4029 5727 5784 6362 6467 1391 Size: 1281 213 1144 1698 57 578 105 4072 Fragments arranged by size: 4072 1698 1281 1144 578 213 105 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2558 3839 4147 6363 928 Size: 971 659 1281 308 2216 3713 Fragments arranged by size: 3713 2216 1281 971 659 308 BbvCI CC'TCA_GC Cuts at: 4086 5265 6246 4086 Size: 1179 981 6988 Fragments arranged by size: 6988 1179 981 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2318 2352 7339 7373 1903 Size: 34 50 34 297 34 4987 34 3678 Fragments arranged by size: 4987 3678 297 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7040 8250 7040 Size: 1210 7938 BclI T'GATC_A Cuts at: 1548 4845 1548 Size: 3297 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5722 7759 136 Size: 107 122 71 631 605 4050 2037 1525 Fragments arranged by size: 4050 2037 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2903 4039 9143 2903 Size: 1136 5104 2908 Fragments arranged by size: 5104 2908 1136 BlpI GC'TnA_GC Cuts at: 4833 6565 4833 Size: 1732 7416 Bme1580I G_kGCm'C Cuts at: 1790 2198 2586 4494 5033 5482 5727 6152 6362 Size: 408 388 1908 539 449 245 425 210 Cuts at: 6362 7209 8365 1790 Size: 847 1156 2573 Fragments arranged by size: 2573 1908 1156 847 539 449 425 408 388 245 210 BmgBI CAC'GTC Cuts at: 1318 3992 1318 Size: 2674 6474 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2970 3042 4168 Size: 416 53 83 399 24 1553 72 1126 Cuts at: 4168 5555 6330 6361 6879 7837 442 Size: 1387 775 31 518 958 1753 Fragments arranged by size: 1753 1553 1387 1126 958 775 518 416 399 83 72 53 31 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9148 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3215 5415 3215 Size: 2200 6948 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2398 3936 4005 4044 4698 5079 5705 7808 Size: 1159 1538 69 39 654 381 626 2103 Cuts at: 7808 1239 Size: 2579 Fragments arranged by size: 2579 2103 1538 1159 654 626 381 69 39 Bpu10I CC'TnA_GC Cuts at: 3014 3315 4086 5265 5390 5778 6205 6246 6334 Size: 301 771 1179 125 388 427 41 88 Cuts at: 6334 3014 Size: 5828 Fragments arranged by size: 5828 1179 771 427 388 301 125 88 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3822 5665 5965 7273 8138 8436 3822 Size: 1843 300 1308 865 298 4534 Fragments arranged by size: 4534 1843 1308 865 300 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3030 3569 4099 5510 5941 6272 7811 Size: 617 1531 539 530 1411 431 331 1539 Cuts at: 7811 882 Size: 2219 Fragments arranged by size: 2219 1539 1531 1411 617 539 530 431 331 BsaAI yAC'GTr Cuts at: 493 1204 6939 493 Size: 711 5735 2702 Fragments arranged by size: 5735 2702 711 BsaBI GATnn'nnATC Cuts at: 9142 9142 Size: 9148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4945 5149 6955 7337 Size: 53 83 186 936 3412 204 1806 382 Cuts at: 7337 7972 275 Size: 635 1451 Fragments arranged by size: 3412 1806 1451 936 635 382 204 186 83 53 BsaWI w'CCGG_w Cuts at: 2439 2592 7581 7979 8253 8400 8811 2439 Size: 153 4989 398 274 147 411 2776 Fragments arranged by size: 4989 2776 411 398 274 153 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 2561 2587 2591 Size: 30 165 30 834 30 294 26 4 Cuts at: 2591 2617 3619 3649 3871 3901 4245 4275 5411 Size: 26 1002 30 222 30 344 30 1136 Cuts at: 5411 5441 1178 Size: 30 4885 Fragments arranged by size: 4885 1136 1002 834 344 294 222 165 30 30 30 30 30 30 30 26 26 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2064 2486 3548 5082 5546 5768 6450 1186 Size: 878 422 1062 1534 464 222 682 3884 Fragments arranged by size: 3884 1534 1062 878 682 464 422 222 BseYI C'CCAG_C Cuts at: 5254 5297 5499 5860 5895 6304 8351 8847 5254 Size: 43 202 361 35 409 2047 496 5555 Fragments arranged by size: 5555 2047 496 409 361 202 43 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2312 6182 1163 Size: 573 576 3870 4129 Fragments arranged by size: 4129 3870 576 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2323 2933 6598 7359 8387 1540 Size: 275 186 322 610 3665 761 1028 2301 Fragments arranged by size: 3665 2301 1028 761 610 322 275 186 BsiHKAI G_wGCw'C Cuts at: 4029 4494 5033 5937 7209 7294 8365 4029 Size: 465 539 904 1272 85 1071 4812 Fragments arranged by size: 4812 1272 1071 904 539 465 85 BsmI GAATG_Cn' Cuts at: 6659 6752 6659 Size: 93 9055 BsmBI CGTCTCn'nnnn_ Cuts at: 2920 2920 Size: 9148 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2586 2672 2885 4029 4494 5033 Size: 399 408 388 86 213 1144 465 539 Cuts at: 5033 5482 5727 5784 5937 6152 6362 6467 7209 Size: 449 245 57 153 215 210 105 742 Cuts at: 7209 7294 8365 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1144 1071 742 539 465 449 408 399 388 245 215 213 210 153 105 86 85 57 BspEI T'CCGG_A Cuts at: 8811 8811 Size: 9148 BspHI T'CATG_A Cuts at: 4521 7037 8767 4521 Size: 2516 1730 4902 Fragments arranged by size: 4902 2516 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 5376 5931 6503 844 Size: 1644 2888 555 572 3489 Fragments arranged by size: 3489 2888 1644 572 555 BsrBI CCG'CTC Cuts at: 1394 1823 7035 1394 Size: 429 5212 3507 Fragments arranged by size: 5212 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7643 7817 65 Size: 1183 6395 174 1396 Fragments arranged by size: 6395 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7792 7979 1700 Size: 110 5982 187 2869 Fragments arranged by size: 5982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3817 96 Size: 3721 5427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9148 BssSI C'ACGA_G Cuts at: 7208 8220 7208 Size: 1012 8136 BstAPI GCAn_nnn'nTGC Cuts at: 794 3230 5555 794 Size: 2436 2325 4387 Fragments arranged by size: 4387 2436 2325 BstBI TT'CG_AA Cuts at: 2465 7952 2465 Size: 5487 3661 BstEII G'GTnAC_C Cuts at: 1184 2170 2874 1184 Size: 986 704 7458 Fragments arranged by size: 7458 986 704 BstXI CCAn_nnnn'nTGG Cuts at: 2161 4881 2161 Size: 2720 6428 BstYI r'GATC_y Cuts at: 1972 2660 2903 3125 4039 5014 6478 6845 7230 Size: 688 243 222 914 975 1464 367 385 Cuts at: 7230 7247 8699 8785 8797 9143 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1464 1452 975 914 688 385 367 346 243 222 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5218 5218 Size: 9148 BtgI C'CryG_G Cuts at: 513 1065 5520 513 Size: 552 4455 4141 Fragments arranged by size: 4455 4141 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8409 Fragments arranged by size: 8409 683 56 BtsI GCAGTG_nn' Cuts at: 5361 6664 7458 7478 5361 Size: 1303 794 20 7031 Fragments arranged by size: 7031 1303 794 20 ClaI AT'CG_AT Cuts at: 1554 2429 1554 Size: 875 8273 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5692 5727 529 Size: 35 5128 35 3950 Fragments arranged by size: 5128 3950 35 35 DraI TTT'AAA Cuts at: 6452 6452 Size: 9148 DraIII CAC_nnn'GTG Cuts at: 6220 6220 Size: 9148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8155 809 Size: 507 6839 1802 Fragments arranged by size: 6839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2731 4219 5917 Size: 54 1425 50 275 18 901 1488 1698 Cuts at: 5917 6595 7484 9113 8 Size: 678 889 1629 43 Fragments arranged by size: 1698 1629 1488 1425 901 889 678 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6595 1537 Size: 275 4783 4090 Fragments arranged by size: 4783 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3116 3594 3687 3954 4659 5172 5762 6421 Size: 1763 478 93 267 705 513 590 659 Cuts at: 6421 7078 1353 Size: 657 3423 Fragments arranged by size: 3423 1763 705 659 657 590 513 478 267 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7735 8109 8255 387 Size: 1064 138 6146 374 146 1280 Fragments arranged by size: 6146 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4027 4027 Size: 9148 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2398 2594 3414 3936 4005 4044 4289 Size: 393 766 196 820 522 69 39 245 Cuts at: 4289 4683 4698 5079 5559 5705 5922 6260 7211 Size: 394 15 381 480 146 217 338 951 Cuts at: 7211 7808 8595 8863 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 951 820 787 766 597 522 480 394 393 381 338 268 245 217 196 146 69 39 15 EcoNI CCTnn'n_nnAGG Cuts at: 2815 2890 2920 6012 2815 Size: 75 30 3092 5951 Fragments arranged by size: 5951 3092 75 30 EcoO109I rG'GnC_Cy Cuts at: 5723 5724 5772 6099 6181 6199 6358 6398 5723 Size: 1 48 327 82 18 159 40 8473 Fragments arranged by size: 8473 327 159 82 48 40 18 1 EcoRI G'AATT_C Cuts at: 4958 6457 6835 4958 Size: 1499 378 7271 Fragments arranged by size: 7271 1499 378 EcoRV GAT'ATC Cuts at: 2230 2445 2230 Size: 215 8933 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2973 3965 5849 2973 Size: 992 1884 6272 Fragments arranged by size: 6272 1884 992 FspI TGC'GCA Cuts at: 7654 7654 Size: 9148 HaeII r_GCGC'y Cuts at: 1678 5844 8295 1678 Size: 4166 2451 2531 Fragments arranged by size: 4166 2531 2451 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2237 2251 2269 2429 Size: 32 135 32 647 18 14 18 160 Cuts at: 2429 2461 2561 2587 2593 2619 2699 2731 3022 Size: 32 100 26 6 26 80 32 291 Cuts at: 3022 3054 3446 3478 3529 3561 3976 4008 4999 Size: 32 392 32 51 32 415 32 991 Cuts at: 4999 5031 7789 7821 7863 7895 1373 Size: 32 2758 32 42 32 2626 Fragments arranged by size: 2758 2626 991 647 415 392 291 160 135 100 80 51 42 32 32 32 32 32 32 32 32 32 32 32 26 26 18 18 14 6 HincII GTy'rAC Cuts at: 669 1657 1938 4366 6492 6738 669 Size: 988 281 2428 2126 246 3079 Fragments arranged by size: 3079 2428 2126 988 281 246 HindIII A'AGCT_T Cuts at: 748 5598 748 Size: 4850 4298 HpaI GTT'AAC Cuts at: 4366 6738 4366 Size: 2372 6776 KpnI G_GTAC'C Cuts at: 4708 6473 4708 Size: 1765 7383 MfeI C'AATT_G Cuts at: 3597 6747 3597 Size: 3150 5998 MluI A'CGCG_T Cuts at: 9024 9024 Size: 9148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2268 3014 3432 5454 8262 8446 1305 Size: 963 746 418 2022 2808 184 2007 Fragments arranged by size: 2808 2022 2007 963 746 418 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2733 4221 5919 10 Size: 54 1425 343 901 1488 1698 3239 Fragments arranged by size: 3239 1698 1488 1425 901 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4356 7106 7465 7624 518 Size: 648 3190 2750 359 159 2042 Fragments arranged by size: 3190 2750 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2964 3227 3854 4877 5036 5335 5413 5452 Size: 1358 263 627 1023 159 299 78 39 Cuts at: 5452 5615 5653 6564 7241 8389 8634 1606 Size: 163 38 911 677 1148 245 2120 Fragments arranged by size: 2120 1358 1148 1023 911 677 627 299 263 245 163 159 78 39 38 NcoI C'CATG_G Cuts at: 513 1065 5520 513 Size: 552 4455 4141 Fragments arranged by size: 4455 4141 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9148 NotI GC'GGCC_GC Cuts at: 6595 6595 Size: 9148 NspI r_CATG'y Cuts at: 1132 1229 5005 5623 6275 6506 8051 1132 Size: 97 3776 618 652 231 1545 2229 Fragments arranged by size: 3776 2229 1545 652 618 231 97 PasI CC'CwG_GG Cuts at: 1348 2191 1348 Size: 843 8305 PciI A'CATG_T Cuts at: 8047 8047 Size: 9148 PflMI CCAn_nnn'nTGG Cuts at: 1108 6085 1108 Size: 4977 4171 PfoI T'CCnGG_A Cuts at: 2663 2800 2663 Size: 137 9011 PmeI GTTT'AAAC Cuts at: 6452 6452 Size: 9148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3115 3147 7217 7249 8730 8762 3115 Size: 32 4070 32 1481 32 3501 Fragments arranged by size: 4070 3501 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 6099 6181 6199 6099 Size: 82 18 9048 Fragments arranged by size: 9048 82 18 PshAI GACnn'nnGTC Cuts at: 1613 2298 2748 2932 1613 Size: 685 450 184 7829 Fragments arranged by size: 7829 685 450 184 PsiI TTA'TAA Cuts at: 6718 6718 Size: 9148 PspOMI G'GGCC_C Cuts at: 5723 6358 5723 Size: 635 8513 PspXI vC'TCGA_Gb Cuts at: 2770 2770 Size: 9148 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5031 5063 5031 Size: 32 9116 PstI C_TGCA'G Cuts at: 830 3241 4981 5376 6500 830 Size: 2411 1740 395 1124 3478 Fragments arranged by size: 3478 2411 1740 1124 395 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9148 PvuII CAG'CTG Cuts at: 3227 3854 4877 5036 5335 5413 5452 5615 5653 Size: 627 1023 159 299 78 39 163 38 Cuts at: 5653 3227 Size: 6722 Fragments arranged by size: 6722 1023 627 299 163 159 78 39 38 SacI G_AGCT'C Cuts at: 4029 4029 Size: 9148 SalI G'TCGA_C Cuts at: 1936 6490 1936 Size: 4554 4594 SanDI GG'GwC_CC Cuts at: 6199 6199 Size: 9148 SapI GCTCTTCn'nnn_ Cuts at: 3687 5172 3687 Size: 1485 7663 SbfI CC_TGCA'GG Cuts at: 6500 6500 Size: 9148 ScaI AGT'ACT Cuts at: 3745 5230 5960 7396 9108 3745 Size: 1485 730 1436 1712 3785 Fragments arranged by size: 3785 1712 1485 1436 730 SfcI C'TryA_G Cuts at: 826 1045 2887 2917 3237 4977 5048 5372 6496 Size: 219 1842 30 320 1740 71 324 1124 Cuts at: 6496 7631 8312 8503 826 Size: 1135 681 191 1471 Fragments arranged by size: 1842 1740 1471 1135 1124 681 324 320 219 191 71 30 SfiI GGCCn_nnn'nGGCC Cuts at: 5722 5722 Size: 9148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9148 SmaI CCC'GGG Cuts at: 1872 2363 6475 1872 Size: 491 4112 4545 Fragments arranged by size: 4545 4112 491 SmlI C'TyrA_G Cuts at: 820 1017 1943 2770 2812 3837 5644 5944 7252 Size: 197 926 827 42 1025 1807 300 1308 Cuts at: 7252 8153 8415 820 Size: 901 262 1553 Fragments arranged by size: 1807 1553 1308 1025 926 901 827 300 262 197 42 SnaBI TAC'GTA Cuts at: 493 6939 493 Size: 6446 2702 SpeI A'CTAG_T Cuts at: 152 4258 152 Size: 4106 5042 SphI G_CATG'C Cuts at: 1132 6506 1132 Size: 5374 3774 SspI AAT'ATT Cuts at: 5 52 7072 5 Size: 47 7020 2081 Fragments arranged by size: 7020 2081 47 StuI AGG'CCT Cuts at: 1517 5313 5942 6345 1517 Size: 3796 629 403 4320 Fragments arranged by size: 4320 3796 629 403 StyI C'CwwG_G Cuts at: 513 1065 3264 4236 5520 6072 6124 6587 513 Size: 552 2199 972 1284 552 52 463 3074 Fragments arranged by size: 3074 2199 1284 972 552 552 463 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2295 2402 2638 2815 2989 7175 7192 Size: 1255 715 107 236 177 174 4186 17 Cuts at: 7192 7345 7530 7975 325 Size: 153 185 445 1498 Fragments arranged by size: 4186 1498 1255 715 445 236 185 177 174 153 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3743 3817 5228 Size: 274 80 33 51 1443 1766 74 1411 Cuts at: 5228 5958 7394 9106 96 Size: 730 1436 1712 138 Fragments arranged by size: 1766 1712 1443 1436 1411 730 274 138 80 74 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2342 2374 3994 4026 4156 4188 4245 4277 4571 Size: 32 1620 32 130 32 57 32 294 Cuts at: 4571 4603 9108 9140 2342 Size: 32 4505 32 2350 Fragments arranged by size: 4505 2350 1620 294 130 57 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5973 1316 Size: 4657 4491 XbaI T'CTAG_A Cuts at: 6484 6484 Size: 9148 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4825 4825 Size: 9148 XhoI C'TCGA_G Cuts at: 1943 2770 2812 1943 Size: 827 42 8279 Fragments arranged by size: 8279 827 42 XmaI C'CCGG_G Cuts at: 1870 2361 6473 1870 Size: 491 4112 4545 Fragments arranged by size: 4545 4112 491 XmnI GAAnn'nnTTC Cuts at: 3948 7277 3948 Size: 3329 5819 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5150 6956 276 Size: 53 83 186 936 3616 1806 2468 Fragments arranged by size: 3616 2468 1806 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI RsrII SacII SexAI SfoI SrfI SwaI