Restriction map for
pFN21AA0872
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SbfI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 845 GTCGAC GT^[AC][GT]AC
AcsI 1453 AAATTC [GA]^AATT[CT]
ApaI 504 GGGCCC GGGCC^C
ApoI 1453 AAATTC [GA]^AATT[CT]
AvaI 34 CCCGGG C^[CT]CG[GA]G
BalI 521 TGGCCA TGG^CCA
BbsI 124 GAAGAC GAAGAC(2/6)
BpuAI 124 GAAGAC GAAGAC(2/6)
BsiEI 843 CGGTCG CG[GA][CT]^CG
BsoBI 34 CCCGGG C^[CT]CG[GA]G
BspLU11I 256 ACATGT A^CATGT
BssSI 517 CTCGTG CACGAG(-5/-1)
DraIII 779 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 534 GACTTCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1555 CTCTTC CTCTTC(1/4)
EcoT22I 1120 ATGCAT ATGCA^T
HgaI 775 GACGC GACGC(5/10)
HincII 845 GTCGAC GT[CT]^[GA]AC
HindII 845 GTCGAC GT[CT]^[GA]AC
Ksp632I 1555 CTCTTC CTCTTC(1/4)
MluNI 521 TGGCCA TGG^CCA
MscI 521 TGGCCA TGG^CCA
MslI 968 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 570 GCTAGC G^CTAGC
NsiI 1120 ATGCAT ATGCA^T
SacI 1459 GAGCTC GAGCT^C
SalI 845 GTCGAC G^TCGAC
SapI 1554 GCTCTTC GCTCTTC(1/4)
ScaI 1577 AGTACT AGT^ACT
SmaI 34 CCCGGG CCC^GGG
Tsp509I 1454 AATT ^AATT
XmaCI 34 CCCGGG C^CCGGG
XmaI 34 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 41 enzymes) #
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AcyI 1100 GGCGCC G[GA]^CG[CT]C
1218 GGCGCC
AfaI 1353 GTAC GT^AC
1578 GTAC
AflIII 256 ACATGT A^C[GA][CT]GT
604 ACACGT
Alw44I 577 GTGCAC G^TGCAC
935 GTGCAC
ApaLI 577 GTGCAC G^TGCAC
935 GTGCAC
BamHI 117 GGATCC G^GATCC
148 GGATCC
BbeI 1100 GGCGCC GGCGC^C
1218 GGCGCC
BbrPI 605 CACGTG CAC^GTG
1411 CACGTG
BbvCI 1473 CCTCAGC CCTCAGC(-5/-2)
472 GCTGAGG
BglI 943 GCCTTTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1012 GCCTACGTGGC
BsaHI 1100 GGCGCC G[GA]^CG[CT]C
1218 GGCGCC
BsgI 436 GTGCAG GTGCAG(16/14)
1204 GTGCAG
BsmBI 1372 CGTCTC CGTCTC(1/5)
773 GAGACG
BsrBI 868 GAGCGG CCGCTC(-3/-3)
1330 GAGCGG
BstAPI 778 GCACTAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
912 GCAGTTGCTGC
BstEII 1443 GGTGACC G^GT[ACGT]ACC
1485 GGTGACC
BstPI 1443 GGTGACC G^GT[ACGT]ACC
1485 GGTGACC
BstYI 117 GGATCC [GA]^GATC[CT]
148 GGATCC
DsaI 404 CCATGG C^C[GA][CT]GG
1085 CCATGG
EcoNI 495 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1146 CCTTGGGGAGG
EcoO65I 1443 GGTGACC G^GT[ACGT]ACC
1485 GGTGACC
Hin1I 1100 GGCGCC G[GA]^CG[CT]C
1218 GGCGCC
HinfI 228 GAGTC G^A[ACGT]TC
1637 GACTC
KasI 1100 GGCGCC G^GCGCC
1218 GGCGCC
MflI 117 GGATCC [GA]^GATC[CT]
148 GGATCC
NaeI 890 GCCGGC GCC^GGC
1097 GCCGGC
NarI 1100 GGCGCC GG^CGCC
1218 GGCGCC
NcoI 404 CCATGG C^CATGG
1085 CCATGG
NgoMIV 890 GCCGGC G^CCGGC
1097 GCCGGC
PflMI 480 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1080 CCATGCCATGG
PleI 1637 GACTC GAGTC(4/5)
228 GAGTC
PmaCI 605 CACGTG CAC^GTG
1411 CACGTG
PmlI 605 CACGTG CAC^GTG
1411 CACGTG
PpuMI 293 GGGACCC [GA]G^G[AT]CC[CT]
713 AGGACCC
PstI 233 CTGCAG CTGCA^G
910 CTGCAG
PvuII 553 CAGCTG CAG^CTG
902 CAGCTG
RsaI 1353 GTAC GT^AC
1578 GTAC
SfoI 1100 GGCGCC GGC^GCC
1218 GGCGCC
SphI 1122 GCATGC GCATG^C
1365 GCATGC
Van91I 480 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1080 CCATGCCATGG
XhoII 117 GGATCC [GA]^GATC[CT]
148 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I