(Circular) (Six-base) MAPSORT of: pFN21AA0872.seq Check: 2987 from: 1 to: 6346 pFN21AA0872 6346 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3491 3491 Size: 6346 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4156 278 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2851 3689 1937 Size: 914 838 4594 Fragments arranged by size: 4594 914 838 Acc65I G'GTAC_C Cuts at: 3667 3667 Size: 6346 AclI AA'CG_TT Cuts at: 1563 4473 4846 1563 Size: 2910 373 3063 Fragments arranged by size: 3063 2910 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4409 5793 6061 1632 Size: 2777 1384 268 1917 Fragments arranged by size: 2777 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6149 AflIII A'CryG_T Cuts at: 1203 2261 2609 5245 6222 1203 Size: 1058 348 2636 977 1327 Fragments arranged by size: 2636 1327 1058 977 348 AgeI A'CCGG_T Cuts at: 5177 5177 Size: 6346 AhdI GACnn_n'nnGTC Cuts at: 5075 5075 Size: 6346 AleI CACnn'nnGTG Cuts at: 1166 2977 1166 Size: 1811 4535 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2995 1184 Size: 1811 4535 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2054 2086 2054 Size: 32 6314 AlwNI CAG_nnn'CTG Cuts at: 2246 2816 2923 3346 4089 5661 2246 Size: 570 107 423 743 1572 2931 Fragments arranged by size: 2931 1572 743 570 423 107 ApaI G_GGCC'C Cuts at: 2513 2513 Size: 6346 ApaLI G'TGCA_C Cuts at: 2582 2940 4403 5559 2582 Size: 358 1463 1156 3369 Fragments arranged by size: 3369 1463 1156 358 ApoI r'AATT_y Cuts at: 1493 3458 3655 3881 4033 6142 1493 Size: 1965 197 226 152 2109 1697 Fragments arranged by size: 2109 1965 1697 226 197 152 AseI AT'TA_AT Cuts at: 160 1032 4900 160 Size: 872 3868 1606 Fragments arranged by size: 3868 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 AvaI C'yCGr_G Cuts at: 1870 1943 2039 3671 1870 Size: 73 96 1632 4545 Fragments arranged by size: 4545 1632 96 73 BamHI G'GATC_C Cuts at: 2122 2153 3676 4043 2122 Size: 31 1523 367 4425 Fragments arranged by size: 4425 1523 367 31 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2741 2971 3065 3105 3137 Size: 325 217 627 954 230 94 40 32 Cuts at: 3137 3223 3667 5122 618 Size: 86 444 1455 1842 Fragments arranged by size: 1842 1455 954 627 444 325 230 217 94 86 40 32 BanII G_rGCy'C Cuts at: 1391 2513 2731 3468 3665 1391 Size: 1122 218 737 197 4072 Fragments arranged by size: 4072 1122 737 218 197 BbeI G_GCGC'C Cuts at: 3109 3227 3109 Size: 118 6228 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2136 928 Size: 971 237 5138 Fragments arranged by size: 5138 971 237 BbvCI CC'TCA_GC Cuts at: 2478 3479 2478 Size: 1001 5345 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4537 4571 1903 Size: 34 2600 34 3678 Fragments arranged by size: 3678 2600 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4238 5448 4238 Size: 1210 5136 BclI T'GATC_A Cuts at: 1548 1548 Size: 6346 BfrBI ATG'CAT Cuts at: 3127 3127 Size: 6346 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2954 3023 4957 Size: 107 122 71 631 605 1282 69 1934 Cuts at: 4957 136 Size: 1525 Fragments arranged by size: 1934 1525 1282 631 605 122 107 71 69 BglII A'GATC_T Cuts at: 6341 6341 Size: 6346 BlpI GC'TnA_GC Cuts at: 3763 3763 Size: 6346 Bme1580I G_kGCm'C Cuts at: 1790 2513 2586 2760 2944 2974 3151 4407 5563 Size: 723 73 174 184 30 177 1256 1156 Cuts at: 5563 1790 Size: 2573 Fragments arranged by size: 2573 1256 1156 723 184 177 174 73 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6346 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2070 2191 2298 Size: 416 53 83 399 24 653 121 107 Cuts at: 2298 2941 3283 4077 5035 442 Size: 643 342 794 958 1753 Fragments arranged by size: 1753 958 794 653 643 416 399 342 121 107 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2579 1056 Size: 1523 4823 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2296 2750 2296 Size: 454 5892 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 2251 2304 5006 1239 Size: 923 89 53 2702 2579 Fragments arranged by size: 2702 2579 923 89 53 Bpu10I CC'TnA_GC Cuts at: 2478 3303 3479 2478 Size: 825 176 5345 Fragments arranged by size: 5345 825 176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4471 5336 5634 4471 Size: 865 298 5183 Fragments arranged by size: 5183 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5009 882 Size: 617 3510 2219 Fragments arranged by size: 3510 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2612 3022 3418 4137 493 Size: 711 1408 410 396 719 2702 Fragments arranged by size: 2702 1408 719 711 410 396 BsaBI GATnn'nnATC Cuts at: 6340 6340 Size: 6346 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3106 3224 4153 4535 Size: 53 83 186 936 1573 118 929 382 Cuts at: 4535 5170 275 Size: 635 1451 Fragments arranged by size: 1573 1451 936 929 635 382 186 118 83 53 BsaWI w'CCGG_w Cuts at: 4779 5177 5451 5598 6009 4779 Size: 398 274 147 411 5116 Fragments arranged by size: 5116 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2384 2414 3374 3404 1178 Size: 30 165 30 981 30 960 30 4120 Fragments arranged by size: 4120 981 960 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2031 2327 2786 3344 3347 3563 1186 Size: 845 296 459 558 3 216 3969 Fragments arranged by size: 3969 845 558 459 296 216 3 BseYI C'CCAG_C Cuts at: 2177 2556 2905 3534 5549 6045 2177 Size: 379 349 629 2015 496 2478 Fragments arranged by size: 2478 2015 629 496 379 349 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2462 3230 1163 Size: 573 726 768 4279 Fragments arranged by size: 4279 768 726 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2851 3796 4557 5585 1540 Size: 275 186 850 945 761 1028 2301 Fragments arranged by size: 2301 1028 945 850 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2586 2603 2944 3196 3468 4407 4492 5563 2586 Size: 17 341 252 272 939 85 1071 3369 Fragments arranged by size: 3369 1071 939 341 272 252 85 17 BsmI GAATG_Cn' Cuts at: 3857 3950 3857 Size: 93 6253 BsmBI CGTCTCn'nnnn_ Cuts at: 2772 3383 2772 Size: 611 5735 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2513 2586 2603 2731 2760 2944 2974 Size: 399 723 73 17 128 29 184 30 Cuts at: 2974 3151 3196 3468 3665 4407 4492 5563 1391 Size: 177 45 272 197 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 723 399 272 197 184 177 128 85 73 45 30 29 17 BspEI T'CCGG_A Cuts at: 6009 6009 Size: 6346 BspHI T'CATG_A Cuts at: 4235 5965 4235 Size: 1730 4616 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2922 3482 3491 3701 844 Size: 2078 560 9 210 3489 Fragments arranged by size: 3489 2078 560 210 9 BsrBI CCG'CTC Cuts at: 1394 1823 2875 3337 4233 1394 Size: 429 1052 462 896 3507 Fragments arranged by size: 3507 1052 896 462 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4841 5015 65 Size: 1183 3593 174 1396 Fragments arranged by size: 3593 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2895 3102 3555 4990 5177 1700 Size: 110 1085 207 453 1435 187 2869 Fragments arranged by size: 2869 1435 1085 453 207 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6346 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6346 BssSI C'ACGA_G Cuts at: 2522 4406 5418 2522 Size: 1884 1012 3450 Fragments arranged by size: 3450 1884 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2789 2923 794 Size: 1995 134 4217 Fragments arranged by size: 4217 1995 134 BstBI TT'CG_AA Cuts at: 5150 5150 Size: 6346 BstEII G'GTnAC_C Cuts at: 1184 3448 3490 1184 Size: 2264 42 4040 Fragments arranged by size: 4040 2264 42 BstYI r'GATC_y Cuts at: 1972 2122 2153 3676 4043 4428 4445 5897 5983 Size: 150 31 1523 367 385 17 1452 86 Cuts at: 5983 5995 6341 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1523 1452 385 367 346 150 86 31 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2409 3090 513 Size: 552 939 405 681 3769 Fragments arranged by size: 3769 939 681 552 405 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5607 Fragments arranged by size: 5607 683 56 BtsI GCAGTG_nn' Cuts at: 2022 3862 4656 4676 2022 Size: 1840 794 20 3692 Fragments arranged by size: 3692 1840 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6346 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6311 DraI TTT'AAA Cuts at: 3650 3650 Size: 6346 DraIII CAC_nnn'GTG Cuts at: 2789 2789 Size: 6346 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2545 5353 809 Size: 507 1229 2808 1802 Fragments arranged by size: 2808 1802 1229 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2526 2886 3093 Size: 54 1425 50 275 18 696 360 207 Cuts at: 3093 3793 4682 6311 8 Size: 700 889 1629 43 Fragments arranged by size: 1629 1425 889 700 696 360 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3793 1537 Size: 275 1981 4090 Fragments arranged by size: 4090 1981 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3566 4276 1353 Size: 2213 710 3423 Fragments arranged by size: 3423 2213 710 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2648 4933 5307 5453 387 Size: 1064 138 1059 2285 374 146 1280 Fragments arranged by size: 2285 1280 1064 1059 374 146 138 EcoICRI GAG'CTC Cuts at: 3466 3466 Size: 6346 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2251 2304 4409 5006 5793 6061 Size: 393 530 89 53 2105 597 787 268 Cuts at: 6061 1239 Size: 1524 Fragments arranged by size: 2105 1524 787 597 530 393 268 89 53 EcoNI CCTnn'n_nnAGG Cuts at: 2504 3155 2504 Size: 651 5695 EcoO109I rG'GnC_Cy Cuts at: 2137 2206 2299 2509 2719 3313 2137 Size: 69 93 210 210 594 5170 Fragments arranged by size: 5170 594 210 210 93 69 EcoRI G'AATT_C Cuts at: 3655 4033 3655 Size: 378 5968 FspI TGC'GCA Cuts at: 4852 4852 Size: 6346 HaeII r_GCGC'y Cuts at: 1678 2399 2816 2828 3109 3227 5493 1678 Size: 721 417 12 281 118 2266 2531 Fragments arranged by size: 2531 2266 721 417 281 118 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2384 2416 2445 Size: 32 135 32 623 32 157 32 29 Cuts at: 2445 2477 4987 5019 5061 5093 1373 Size: 32 2510 32 42 32 2626 Fragments arranged by size: 2626 2510 623 157 135 42 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 2852 3690 3936 669 Size: 988 281 914 838 246 3079 Fragments arranged by size: 3079 988 914 838 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6346 HpaI GTT'AAC Cuts at: 3936 3936 Size: 6346 KasI G'GCGC_C Cuts at: 3105 3223 3105 Size: 118 6228 KpnI G_GTAC'C Cuts at: 3671 3671 Size: 6346 MfeI C'AATT_G Cuts at: 3945 3945 Size: 6346 MluI A'CGCG_T Cuts at: 6222 6222 Size: 6346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1993 2824 3330 5460 5644 1305 Size: 688 831 506 2130 184 2007 Fragments arranged by size: 2130 2007 831 688 506 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2528 10 Size: 54 1425 343 696 3828 Fragments arranged by size: 3828 1425 696 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2977 4304 4663 4822 518 Size: 648 1811 1327 359 159 2042 Fragments arranged by size: 2042 1811 1327 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2329 2560 2773 2909 3256 3343 3762 4439 Size: 723 231 213 136 347 87 419 677 Cuts at: 4439 5587 5832 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 723 677 419 347 245 231 213 136 87 NaeI GCC'GGC Cuts at: 2897 3104 2897 Size: 207 6139 NarI GG'CG_CC Cuts at: 3106 3224 3106 Size: 118 6228 NcoI C'CATG_G Cuts at: 513 1065 2004 2409 3090 513 Size: 552 939 405 681 3769 Fragments arranged by size: 3769 939 681 552 405 NdeI CA'TA_TG Cuts at: 387 387 Size: 6346 NgoMIV G'CCGG_C Cuts at: 2895 3102 2895 Size: 207 6139 NheI G'CTAG_C Cuts at: 1052 2575 1052 Size: 1523 4823 NotI GC'GGCC_GC Cuts at: 3793 3793 Size: 6346 NsiI A_TGCA'T Cuts at: 3129 3129 Size: 6346 NspI r_CATG'y Cuts at: 1132 1229 2265 2339 3131 3374 3704 5249 1132 Size: 97 1036 74 792 243 330 1545 2229 Fragments arranged by size: 2229 1545 1036 792 330 243 97 74 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6346 PciI A'CATG_T Cuts at: 2261 5245 2261 Size: 2984 3362 PflMI CCAn_nnn'nTGG Cuts at: 1108 2491 3091 1108 Size: 1383 600 4363 Fragments arranged by size: 4363 1383 600 PmeI GTTT'AAAC Cuts at: 3650 3650 Size: 6346 PmlI CAC'GTG Cuts at: 2612 3418 2612 Size: 806 5540 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4415 4447 5928 5960 4415 Size: 32 1481 32 4801 Fragments arranged by size: 4801 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2299 2719 2299 Size: 420 5926 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6346 PsiI TTA'TAA Cuts at: 2858 3916 2858 Size: 1058 5288 PspOMI G'GGCC_C Cuts at: 2509 2509 Size: 6346 PstI C_TGCA'G Cuts at: 830 2242 2919 3698 830 Size: 1412 677 779 3478 Fragments arranged by size: 3478 1412 779 677 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6346 PvuII CAG'CTG Cuts at: 2560 2909 2560 Size: 349 5997 SacI G_AGCT'C Cuts at: 3468 3468 Size: 6346 SalI G'TCGA_C Cuts at: 1936 2850 3688 1936 Size: 914 838 4594 Fragments arranged by size: 4594 914 838 SanDI GG'GwC_CC Cuts at: 2299 2299 Size: 6346 SapI GCTCTTCn'nnn_ Cuts at: 3566 3566 Size: 6346 SbfI CC_TGCA'GG Cuts at: 3698 3698 Size: 6346 ScaI AGT'ACT Cuts at: 3584 4594 6306 3584 Size: 1010 1712 3624 Fragments arranged by size: 3624 1712 1010 SfcI C'TryA_G Cuts at: 826 1045 2238 2915 3202 3319 3694 4829 5510 Size: 219 1193 677 287 117 375 1135 681 Cuts at: 5510 5701 826 Size: 191 1471 Fragments arranged by size: 1471 1193 1135 681 677 375 287 219 191 117 SfoI GGC'GCC Cuts at: 3107 3225 3107 Size: 118 6228 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6346 SmaI CCC'GGG Cuts at: 1872 2041 3673 1872 Size: 169 1632 4545 Fragments arranged by size: 4545 1632 169 SmlI C'TyrA_G Cuts at: 820 1017 1943 4450 5351 5613 820 Size: 197 926 2507 901 262 1553 Fragments arranged by size: 2507 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4137 493 Size: 3644 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6346 SphI G_CATG'C Cuts at: 1132 3131 3374 3704 1132 Size: 1999 243 330 3774 Fragments arranged by size: 3774 1999 330 243 SspI AAT'ATT Cuts at: 5 52 4270 5 Size: 47 4218 2081 Fragments arranged by size: 4218 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6346 StyI C'CwwG_G Cuts at: 513 1065 2004 2157 2409 3090 3133 3151 3495 Size: 552 939 153 252 681 43 18 344 Cuts at: 3495 3785 513 Size: 290 3074 Fragments arranged by size: 3074 939 681 552 344 290 252 153 43 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2191 2402 2709 2729 2923 2927 3083 Size: 1255 611 211 307 20 194 4 156 Cuts at: 3083 3436 3526 3592 4373 4390 4543 4728 5173 Size: 353 90 66 781 17 153 185 445 Cuts at: 5173 325 Size: 1498 Fragments arranged by size: 1498 1255 781 611 445 353 307 211 194 185 156 153 90 66 20 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3582 4592 6304 Size: 274 80 33 51 1443 1605 1010 1712 Cuts at: 6304 96 Size: 138 Fragments arranged by size: 1712 1605 1443 1010 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3374 3406 6306 6338 3374 Size: 32 2900 32 3382 Fragments arranged by size: 3382 2900 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6346 XbaI T'CTAG_A Cuts at: 3682 3682 Size: 6346 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6346 XmaI C'CCGG_G Cuts at: 1870 2039 3671 1870 Size: 169 1632 4545 Fragments arranged by size: 4545 1632 169 XmnI GAAnn'nnTTC Cuts at: 4475 4475 Size: 6346 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4154 276 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI PacI PfoI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI