Restriction map for
pFN21AA0872
(Linear) (Six-base) MAPSORT of: KIAA0872.seq Check: 7111 from: 1 to: 1641
KIAA0872.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1486 1641
Size: 1486 155
AccI GT'mk_AC
Cuts at: 0 846 1641
Size: 846 795
AflIII A'CryG_T
Cuts at: 0 256 604 1641
Size: 256 348 1037
Fragments arranged by size:
1037 348 256
AleI CACnn'nnGTG
Cuts at: 0 972 1641
Size: 972 669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 990 1641
Size: 990 651
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 49 81 1641
Size: 49 32 1560
Fragments arranged by size:
1560 49 32
AlwNI CAG_nnn'CTG
Cuts at: 0 241 811 918 1341 1641
Size: 241 570 107 423 300
Fragments arranged by size:
570 423 300 241 107
ApaI G_GGCC'C
Cuts at: 0 508 1641
Size: 508 1133
ApaLI G'TGCA_C
Cuts at: 0 577 935 1641
Size: 577 358 706
Fragments arranged by size:
706 577 358
ApoI r'AATT_y
Cuts at: 0 1453 1641
Size: 1453 188
AvaI C'yCGr_G
Cuts at: 0 34 1641
Size: 34 1607
BamHI G'GATC_C
Cuts at: 0 117 148 1641
Size: 117 31 1493
Fragments arranged by size:
1493 117 31
BanI G'GyrC_C
Cuts at: 0 736 966 1060 1100 1132 1218 1641
Size: 736 230 94 40 32 86 423
Fragments arranged by size:
736 423 230 94 86 40 32
BanII G_rGCy'C
Cuts at: 0 508 726 1463 1641
Size: 508 218 737 178
Fragments arranged by size:
737 508 218 178
BbeI G_GCGC'C
Cuts at: 0 1104 1222 1641
Size: 1104 118 419
Fragments arranged by size:
1104 419 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 1641
Size: 131 1510
BbvCI CC'TCA_GC
Cuts at: 0 473 1474 1641
Size: 473 1001 167
Fragments arranged by size:
1001 473 167
BfrBI ATG'CAT
Cuts at: 0 1122 1641
Size: 1122 519
BglI GCCn_nnn'nGGC
Cuts at: 0 949 1018 1641
Size: 949 69 623
Fragments arranged by size:
949 623 69
Bme1580I G_kGCm'C
Cuts at: 0 508 581 755 939 969 1146 1641
Size: 508 73 174 184 30 177 495
Fragments arranged by size:
508 495 184 177 174 73 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 65 186 293 936 1278 1641
Size: 65 121 107 643 342 363
Fragments arranged by size:
643 363 342 121 107 65
BmtI G_CTAG'C
Cuts at: 0 574 1641
Size: 574 1067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 291 745 1641
Size: 291 454 896
Fragments arranged by size:
896 454 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 246 299 1641
Size: 157 89 53 1342
Fragments arranged by size:
1342 157 89 53
Bpu10I CC'TnA_GC
Cuts at: 0 473 1298 1474 1641
Size: 473 825 176 167
Fragments arranged by size:
825 473 176 167
BsaAI yAC'GTr
Cuts at: 0 607 1017 1413 1641
Size: 607 410 396 228
Fragments arranged by size:
607 410 396 228
BsaHI Gr'CG_yC
Cuts at: 0 1101 1219 1641
Size: 1101 118 422
Fragments arranged by size:
1101 422 118
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 379 409 1369 1399 1641
Size: 379 30 960 30 242
Fragments arranged by size:
960 379 242 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 26 322 781 1339 1342 1558 1641
Size: 26 296 459 558 3 216 83
Fragments arranged by size:
558 459 296 216 83 26 3
BseYI C'CCAG_C
Cuts at: 0 172 551 900 1529 1641
Size: 172 379 349 629 112
Fragments arranged by size:
629 379 349 172 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1225 1641
Size: 457 768 416
Fragments arranged by size:
768 457 416
BsiEI CG_ry'CG
Cuts at: 0 846 1641
Size: 846 795
BsiHKAI G_wGCw'C
Cuts at: 0 581 598 939 1191 1463 1641
Size: 581 17 341 252 272 178
Fragments arranged by size:
581 341 272 252 178 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 767 1378 1641
Size: 767 611 263
Fragments arranged by size:
767 611 263
Bsp1286I G_dGCh'C
Cuts at: 0 508 581 598 726 755 939 969 1146
Size: 508 73 17 128 29 184 30 177
Cuts at: 1146 1191 1463 1641
Size: 45 272 178
Fragments arranged by size:
508 272 184 178 177 128 73 45
30 29 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 917 1477 1486 1641
Size: 917 560 9 155
Fragments arranged by size:
917 560 155 9
BsrBI CCG'CTC
Cuts at: 0 870 1332 1641
Size: 870 462 309
Fragments arranged by size:
870 462 309
BsrFI r'CCGG_y
Cuts at: 0 890 1097 1550 1641
Size: 890 207 453 91
Fragments arranged by size:
890 453 207 91
BssSI C'ACGA_G
Cuts at: 0 517 1641
Size: 517 1124
BstAPI GCAn_nnn'nTGC
Cuts at: 0 784 918 1641
Size: 784 134 723
Fragments arranged by size:
784 723 134
BstEII G'GTnAC_C
Cuts at: 0 1443 1485 1641
Size: 1443 42 156
Fragments arranged by size:
1443 156 42
BstYI r'GATC_y
Cuts at: 0 117 148 1641
Size: 117 31 1493
Fragments arranged by size:
1493 117 31
BtgI C'CryG_G
Cuts at: 0 404 1085 1641
Size: 404 681 556
Fragments arranged by size:
681 556 404
BtsI GCAGTG_nn'
Cuts at: 0 17 1641
Size: 17 1624
DraIII CAC_nnn'GTG
Cuts at: 0 784 1641
Size: 784 857
DrdI GACnn_nn'nnGTC
Cuts at: 0 540 1641
Size: 540 1101
EaeI y'GGCC_r
Cuts at: 0 521 881 1088 1641
Size: 521 360 207 553
Fragments arranged by size:
553 521 360 207
EarI CTCTTCn'nnn_
Cuts at: 0 1561 1641
Size: 1561 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 643 1641
Size: 643 998
EcoICRI GAG'CTC
Cuts at: 0 1461 1641
Size: 1461 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 246 299 1641
Size: 157 89 53 1342
Fragments arranged by size:
1342 157 89 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 499 1150 1641
Size: 499 651 491
Fragments arranged by size:
651 499 491
EcoO109I rG'GnC_Cy
Cuts at: 0 132 201 294 504 714 1308 1641
Size: 132 69 93 210 210 594 333
Fragments arranged by size:
594 333 210 210 132 93 69
HaeII r_GCGC'y
Cuts at: 0 394 811 823 1104 1222 1641
Size: 394 417 12 281 118 419
Fragments arranged by size:
419 417 394 281 118 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 379 411 440 472 1641
Size: 190 32 157 32 29 32 1169
Fragments arranged by size:
1169 190 157 32 32 32 29
HincII GTy'rAC
Cuts at: 0 847 1641
Size: 847 794
KasI G'GCGC_C
Cuts at: 0 1100 1218 1641
Size: 1100 118 423
Fragments arranged by size:
1100 423 118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 1325 1641
Size: 819 506 316
Fragments arranged by size:
819 506 316
MscI TGG'CCA
Cuts at: 0 523 1641
Size: 523 1118
MslI CAynn'nnrTG
Cuts at: 0 972 1641
Size: 972 669
MspA1I CmG'CkG
Cuts at: 0 324 555 768 904 1251 1338 1641
Size: 324 231 213 136 347 87 303
Fragments arranged by size:
347 324 303 231 213 136 87
NaeI GCC'GGC
Cuts at: 0 892 1099 1641
Size: 892 207 542
Fragments arranged by size:
892 542 207
NarI GG'CG_CC
Cuts at: 0 1101 1219 1641
Size: 1101 118 422
Fragments arranged by size:
1101 422 118
NcoI C'CATG_G
Cuts at: 0 404 1085 1641
Size: 404 681 556
Fragments arranged by size:
681 556 404
NgoMIV G'CCGG_C
Cuts at: 0 890 1097 1641
Size: 890 207 544
Fragments arranged by size:
890 544 207
NheI G'CTAG_C
Cuts at: 0 570 1641
Size: 570 1071
NsiI A_TGCA'T
Cuts at: 0 1124 1641
Size: 1124 517
NspI r_CATG'y
Cuts at: 0 260 334 1126 1369 1641
Size: 260 74 792 243 272
Fragments arranged by size:
792 272 260 243 74
PciI A'CATG_T
Cuts at: 0 256 1641
Size: 256 1385
PflMI CCAn_nnn'nTGG
Cuts at: 0 486 1086 1641
Size: 486 600 555
Fragments arranged by size:
600 555 486
PmlI CAC'GTG
Cuts at: 0 607 1413 1641
Size: 607 806 228
Fragments arranged by size:
806 607 228
PpuMI rG'GwC_Cy
Cuts at: 0 294 714 1641
Size: 294 420 927
Fragments arranged by size:
927 420 294
PsiI TTA'TAA
Cuts at: 0 853 1641
Size: 853 788
PspOMI G'GGCC_C
Cuts at: 0 504 1641
Size: 504 1137
PstI C_TGCA'G
Cuts at: 0 237 914 1641
Size: 237 677 727
Fragments arranged by size:
727 677 237
PvuII CAG'CTG
Cuts at: 0 555 904 1641
Size: 555 349 737
Fragments arranged by size:
737 555 349
SacI G_AGCT'C
Cuts at: 0 1463 1641
Size: 1463 178
SalI G'TCGA_C
Cuts at: 0 845 1641
Size: 845 796
SanDI GG'GwC_CC
Cuts at: 0 294 1641
Size: 294 1347
SapI GCTCTTCn'nnn_
Cuts at: 0 1561 1641
Size: 1561 80
ScaI AGT'ACT
Cuts at: 0 1579 1641
Size: 1579 62
SfcI C'TryA_G
Cuts at: 0 233 910 1197 1314 1641
Size: 233 677 287 117 327
Fragments arranged by size:
677 327 287 233 117
SfoI GGC'GCC
Cuts at: 0 1102 1220 1641
Size: 1102 118 421
Fragments arranged by size:
1102 421 118
SmaI CCC'GGG
Cuts at: 0 36 1641
Size: 36 1605
SphI G_CATG'C
Cuts at: 0 1126 1369 1641
Size: 1126 243 272
Fragments arranged by size:
1126 272 243
StyI C'CwwG_G
Cuts at: 0 152 404 1085 1128 1146 1490 1641
Size: 152 252 681 43 18 344 151
Fragments arranged by size:
681 344 252 152 151 43 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 397 704 724 918 922 1078 1431
Size: 186 211 307 20 194 4 156 353
Cuts at: 1431 1521 1587 1641
Size: 90 66 54
Fragments arranged by size:
353 307 211 194 186 156 90 66
54 20 4
TatI w'GTAC_w
Cuts at: 0 1577 1641
Size: 1577 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1369 1401 1641
Size: 1369 32 240
Fragments arranged by size:
1369 240 32
XmaI C'CCGG_G
Cuts at: 0 34 1641
Size: 34 1607
Enzymes that do cut:
AarI AccI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BfrBI BglI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI
BstEII BstYI BtgI BtsI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PciI PflMI PmlI PpuMI
PsiI PspOMI PstI PvuII SacI SalI SanDI SapI
ScaI SfcI SfoI SmaI SphI StyI TaqII TatI
TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI
BglII BlpI BmgBI BpuEI BsaI BsaBI BsaWI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NdeI NotI NruI PacI PasI PfoI
PmeI PpiI PshAI PspXI PsrI PvuI RsrII SacII
SbfI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmnI ZraI