(Circular) (Six-base) MAPSORT of: pFN21AA0868.seq Check: 2090 from: 1 to: 8698 pFN21AA0868 8698 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3779 4305 3779 Size: 526 8172 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2660 6508 278 Size: 53 83 186 936 1124 3848 2468 Fragments arranged by size: 3848 2468 1124 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5103 5310 6041 1937 Size: 3166 207 731 4594 Fragments arranged by size: 4594 3166 731 207 Acc65I G'GTAC_C Cuts at: 6019 6019 Size: 8698 AclI AA'CG_TT Cuts at: 1563 6825 7198 1563 Size: 5262 373 3063 Fragments arranged by size: 5262 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2243 3223 3527 4574 5146 5152 6761 8145 Size: 611 980 304 1047 572 6 1609 1384 Cuts at: 8145 8413 1632 Size: 268 1917 Fragments arranged by size: 1917 1609 1384 1047 980 611 572 304 268 6 AfeI AGC'GCT Cuts at: 2040 4465 2040 Size: 2425 6273 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8501 AflIII A'CryG_T Cuts at: 1203 3654 5859 7597 8574 1203 Size: 2451 2205 1738 977 1327 Fragments arranged by size: 2451 2205 1738 1327 977 AgeI A'CCGG_T Cuts at: 7529 7529 Size: 8698 AhdI GACnn_n'nnGTC Cuts at: 7427 7427 Size: 8698 AleI CACnn'nnGTG Cuts at: 1166 2795 4568 1166 Size: 1629 1773 5296 Fragments arranged by size: 5296 1773 1629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2307 1184 Size: 1123 7575 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3445 3477 3445 Size: 32 8666 AlwNI CAG_nnn'CTG Cuts at: 2064 2079 3184 3356 6441 8013 2064 Size: 15 1105 172 3085 1572 2749 Fragments arranged by size: 3085 2749 1572 1105 172 15 ApaLI G'TGCA_C Cuts at: 5833 6755 7911 5833 Size: 922 1156 6620 Fragments arranged by size: 6620 1156 922 ApoI r'AATT_y Cuts at: 1493 2998 3091 3406 3598 6007 6233 6385 8494 Size: 1505 93 315 192 2409 226 152 2109 Cuts at: 8494 1493 Size: 1697 Fragments arranged by size: 2409 2109 1697 1505 315 226 192 152 93 AseI AT'TA_AT Cuts at: 160 1032 7252 160 Size: 872 6220 1606 Fragments arranged by size: 6220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8698 AvaI C'yCGr_G Cuts at: 1870 1943 4894 5286 6023 1870 Size: 73 2951 392 737 4545 Fragments arranged by size: 4545 2951 737 392 73 AvrII C'CTAG_G Cuts at: 3817 3817 Size: 8698 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2887 2920 4945 4978 2887 Size: 33 2025 33 6607 Fragments arranged by size: 6607 2025 33 33 BamHI G'GATC_C Cuts at: 6028 6395 6028 Size: 367 8331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3124 3160 4650 5065 5182 Size: 325 217 627 1337 36 1490 415 117 Cuts at: 5182 5282 5697 5876 6019 7474 618 Size: 100 415 179 143 1455 1842 Fragments arranged by size: 1842 1490 1455 1337 627 415 415 325 217 179 143 117 100 36 BanII G_rGCy'C Cuts at: 1391 3078 4758 6017 1391 Size: 1687 1680 1259 4072 Fragments arranged by size: 4072 1687 1680 1259 BbeI G_GCGC'C Cuts at: 5186 5186 Size: 8698 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3229 5375 5836 928 Size: 971 1330 2146 461 3790 Fragments arranged by size: 3790 2146 1330 971 461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3368 3402 6889 6923 1903 Size: 34 50 34 1347 34 3487 34 3678 Fragments arranged by size: 3678 3487 1347 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 6590 7800 3749 Size: 2841 1210 4647 Fragments arranged by size: 4647 2841 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8698 BfrBI ATG'CAT Cuts at: 3564 3564 Size: 8698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2084 3169 Size: 107 122 71 631 605 337 75 1085 Cuts at: 3169 4797 5874 7309 136 Size: 1628 1077 1435 1525 Fragments arranged by size: 1628 1525 1435 1085 1077 631 605 337 122 107 75 71 BglII A'GATC_T Cuts at: 8693 8693 Size: 8698 BlpI GC'TnA_GC Cuts at: 6115 6115 Size: 8698 Bme1580I G_kGCm'C Cuts at: 1790 3165 3684 5837 5879 6759 7915 1790 Size: 1375 519 2153 42 880 1156 2573 Fragments arranged by size: 2573 2153 1375 1156 880 519 42 BmgBI CAC'GTC Cuts at: 1318 2097 5611 1318 Size: 779 3514 4405 Fragments arranged by size: 4405 3514 779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2563 4165 6429 Size: 416 53 83 399 24 1146 1602 2264 Cuts at: 6429 7387 442 Size: 958 1753 Fragments arranged by size: 2264 1753 1602 1146 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2968 3176 4568 4772 4871 5074 5527 7358 Size: 1729 208 1392 204 99 203 453 1831 Cuts at: 7358 1239 Size: 2579 Fragments arranged by size: 2579 1831 1729 1392 453 208 204 203 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3511 4843 5366 5558 5605 6823 7688 7986 3511 Size: 1332 523 192 47 1218 865 298 4223 Fragments arranged by size: 4223 1332 1218 865 523 298 192 47 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2232 3233 5957 7361 882 Size: 617 733 1001 2724 1404 2219 Fragments arranged by size: 2724 2219 1404 1001 733 617 BsaAI yAC'GTr Cuts at: 493 1204 6489 493 Size: 711 5285 2702 Fragments arranged by size: 5285 2702 711 BsaBI GATnn'nnATC Cuts at: 5394 8692 5394 Size: 3298 5400 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2657 3036 3947 5183 Size: 53 83 186 936 1124 379 911 1236 Cuts at: 5183 5802 5928 6505 6887 7522 275 Size: 619 126 577 382 635 1451 Fragments arranged by size: 1451 1236 1124 936 911 635 619 577 382 379 186 126 83 53 BsaWI w'CCGG_w Cuts at: 2343 4886 5853 7131 7529 7803 7950 8361 2343 Size: 2543 967 1278 398 274 147 411 2680 Fragments arranged by size: 2680 2543 1278 967 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3989 4019 4409 4439 4541 Size: 30 165 30 2586 30 390 30 102 Cuts at: 4541 4571 4949 4979 5192 5222 1178 Size: 30 378 30 213 30 4654 Fragments arranged by size: 4654 2586 390 378 213 165 102 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2146 2149 2223 5165 5186 1186 Size: 960 3 74 2942 21 4698 Fragments arranged by size: 4698 2942 960 74 21 3 BseYI C'CCAG_C Cuts at: 2216 2978 4784 7901 8397 2216 Size: 762 1806 3117 496 2517 Fragments arranged by size: 3117 2517 1806 762 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2666 4195 4418 1163 Size: 573 930 1529 223 5443 Fragments arranged by size: 5443 1529 930 573 223 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6148 6909 7937 1540 Size: 275 186 4147 761 1028 2301 Fragments arranged by size: 4147 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3713 4758 5837 6759 6844 7915 3713 Size: 1045 1079 922 85 1071 4496 Fragments arranged by size: 4496 1079 1071 1045 922 85 BsmI GAATG_Cn' Cuts at: 2833 4405 5190 6209 6302 2833 Size: 1572 785 1019 93 5229 Fragments arranged by size: 5229 1572 1019 785 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3078 3165 3684 3713 4758 5837 5879 Size: 399 1288 87 519 29 1045 1079 42 Cuts at: 5879 6017 6759 6844 7915 1391 Size: 138 742 85 1071 2174 Fragments arranged by size: 2174 1288 1079 1071 1045 742 519 399 138 87 85 42 29 BspEI T'CCGG_A Cuts at: 4886 8361 4886 Size: 3475 5223 BspHI T'CATG_A Cuts at: 3928 5937 6587 8317 3928 Size: 2009 650 1730 4309 Fragments arranged by size: 4309 2009 1730 650 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3779 4305 4440 6053 844 Size: 2935 526 135 1613 3489 Fragments arranged by size: 3489 2935 1613 526 135 BsrBI CCG'CTC Cuts at: 1394 1823 4818 6585 1394 Size: 429 2995 1767 3507 Fragments arranged by size: 3507 2995 1767 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2295 2637 3271 4262 4900 5805 7193 Size: 1183 1047 342 634 991 638 905 1388 Cuts at: 7193 7367 65 Size: 174 1396 Fragments arranged by size: 1396 1388 1183 1047 991 905 638 634 342 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2024 4701 7342 7529 1700 Size: 110 214 2677 2641 187 2869 Fragments arranged by size: 2869 2677 2641 214 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8698 BssHII G'CGCG_C Cuts at: 1925 2020 1925 Size: 95 8603 BssSI C'ACGA_G Cuts at: 3380 5363 6758 7770 3380 Size: 1983 1395 1012 4308 Fragments arranged by size: 4308 1983 1395 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5078 794 Size: 4284 4414 BstBI TT'CG_AA Cuts at: 3451 7502 3451 Size: 4051 4647 BstEII G'GTnAC_C Cuts at: 1184 2332 4620 1184 Size: 1148 2288 5262 Fragments arranged by size: 5262 2288 1148 BstYI r'GATC_y Cuts at: 1972 3847 4216 4716 5154 6028 6395 6780 6797 Size: 1875 369 500 438 874 367 385 17 Cuts at: 6797 8249 8335 8347 8693 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1875 1452 874 500 438 385 369 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3349 3349 Size: 8698 BtgI C'CryG_G Cuts at: 513 1065 4008 4957 513 Size: 552 2943 949 4254 Fragments arranged by size: 4254 2943 949 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7959 Fragments arranged by size: 7959 683 56 BtsI GCAGTG_nn' Cuts at: 4659 6214 7008 7028 4659 Size: 1555 794 20 6329 Fragments arranged by size: 6329 1555 794 20 ClaI AT'CG_AT Cuts at: 1554 3339 3426 1554 Size: 1785 87 6826 Fragments arranged by size: 6826 1785 87 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5260 5295 529 Size: 35 4696 35 3932 Fragments arranged by size: 4696 3932 35 35 DraI TTT'AAA Cuts at: 6002 6002 Size: 8698 DraIII CAC_nnn'GTG Cuts at: 4122 4122 Size: 8698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2438 3922 7705 809 Size: 507 1122 1484 3783 1802 Fragments arranged by size: 3783 1802 1484 1122 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2578 2785 5325 Size: 54 1425 50 275 18 748 207 2540 Cuts at: 5325 6145 7034 8663 8 Size: 820 889 1629 43 Fragments arranged by size: 2540 1629 1425 889 820 748 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6145 1537 Size: 275 4333 4090 Fragments arranged by size: 4333 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5444 6628 1353 Size: 4091 1184 3423 Fragments arranged by size: 4091 3423 1184 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2875 5671 5855 5921 7285 7659 Size: 1064 138 1286 2796 184 66 1364 374 Cuts at: 7659 7805 387 Size: 146 1280 Fragments arranged by size: 2796 1364 1286 1280 1064 374 184 146 138 66 EcoICRI GAG'CTC Cuts at: 4756 4756 Size: 8698 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2968 3176 3223 3527 4568 4574 Size: 393 611 725 208 47 304 1041 6 Cuts at: 4574 4772 4871 5074 5146 5152 5527 6761 7358 Size: 198 99 203 72 6 375 1234 597 Cuts at: 7358 8145 8413 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1234 1041 787 725 611 597 393 375 304 268 208 203 198 99 72 47 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 3398 5144 3398 Size: 1746 6952 EcoO109I rG'GnC_Cy Cuts at: 3181 3872 4364 4726 5649 3181 Size: 691 492 362 923 6230 Fragments arranged by size: 6230 923 691 492 362 EcoRI G'AATT_C Cuts at: 6007 6385 6007 Size: 378 8320 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5392 5476 5599 5392 Size: 84 123 8491 Fragments arranged by size: 8491 123 84 FspI TGC'GCA Cuts at: 2483 7204 2483 Size: 4721 3977 FspAI rTGC'GCAy Cuts at: 2483 2483 Size: 8698 HaeII r_GCGC'y Cuts at: 1678 2042 2854 4005 4467 5186 7845 1678 Size: 364 812 1151 462 719 2659 2531 Fragments arranged by size: 2659 2531 1151 812 719 462 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3173 3205 3332 3364 3425 Size: 32 135 32 1601 32 127 32 61 Cuts at: 3425 3457 4348 4380 4541 4573 4851 4883 5398 Size: 32 891 32 161 32 278 32 515 Cuts at: 5398 5430 5554 5586 7339 7371 7413 7445 1373 Size: 32 124 32 1753 32 42 32 2626 Fragments arranged by size: 2626 1753 1601 891 515 278 161 135 127 124 61 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2266 3040 5350 5776 6042 6288 Size: 988 281 328 774 2310 426 266 246 Cuts at: 6288 669 Size: 3079 Fragments arranged by size: 3079 2310 988 774 426 328 281 266 246 HindIII A'AGCT_T Cuts at: 748 5254 748 Size: 4506 4192 HpaI GTT'AAC Cuts at: 6288 6288 Size: 8698 KasI G'GCGC_C Cuts at: 5182 5182 Size: 8698 KpnI G_GTAC'C Cuts at: 6023 6023 Size: 8698 MfeI C'AATT_G Cuts at: 6297 6297 Size: 8698 MluI A'CGCG_T Cuts at: 8574 8574 Size: 8698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4300 5454 5664 7812 7996 1305 Size: 2995 1154 210 2148 184 2007 Fragments arranged by size: 2995 2148 2007 1154 210 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2580 2787 5327 10 Size: 54 1425 343 748 207 2540 3381 Fragments arranged by size: 3381 2540 1425 748 343 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 2391 2795 3578 3653 4568 5810 6656 Size: 648 1225 404 783 75 915 1242 846 Cuts at: 6656 7015 7174 518 Size: 359 159 2042 Fragments arranged by size: 2042 1242 1225 915 846 783 648 404 359 159 75 MspA1I CmG'CkG Cuts at: 1606 2064 3861 5659 6114 6791 7939 8184 1606 Size: 458 1797 1798 455 677 1148 245 2120 Fragments arranged by size: 2120 1798 1797 1148 677 458 455 245 NaeI GCC'GGC Cuts at: 2026 2026 Size: 8698 NarI GG'CG_CC Cuts at: 5183 5183 Size: 8698 NcoI C'CATG_G Cuts at: 513 1065 4008 513 Size: 552 2943 5203 Fragments arranged by size: 5203 2943 552 NdeI CA'TA_TG Cuts at: 387 4990 387 Size: 4603 4095 NgoMIV G'CCGG_C Cuts at: 2024 2024 Size: 8698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8698 NotI GC'GGCC_GC Cuts at: 6145 6145 Size: 8698 NruI TCG'CGA Cuts at: 3638 3638 Size: 8698 NsiI A_TGCA'T Cuts at: 3566 3566 Size: 8698 NspI r_CATG'y Cuts at: 1132 1229 2893 3568 3658 5863 6056 7601 1132 Size: 97 1664 675 90 2205 193 1545 2229 Fragments arranged by size: 2229 2205 1664 1545 675 193 97 90 PasI CC'CwG_GG Cuts at: 1348 4500 1348 Size: 3152 5546 PciI A'CATG_T Cuts at: 3654 5859 7597 3654 Size: 2205 1738 4755 Fragments arranged by size: 4755 2205 1738 PflMI CCAn_nnn'nTGG Cuts at: 1108 4315 1108 Size: 3207 5491 PfoI T'CCnGG_A Cuts at: 2413 5743 2413 Size: 3330 5368 PmeI GTTT'AAAC Cuts at: 6002 6002 Size: 8698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6767 6799 8280 8312 6767 Size: 32 1481 32 7153 Fragments arranged by size: 7153 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3181 4364 3181 Size: 1183 7515 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8698 PsiI TTA'TAA Cuts at: 6268 6268 Size: 8698 PstI C_TGCA'G Cuts at: 830 2075 4666 6050 830 Size: 1245 2591 1384 3478 Fragments arranged by size: 3478 2591 1384 1245 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8698 PvuII CAG'CTG Cuts at: 2064 2064 Size: 8698 SacI G_AGCT'C Cuts at: 4758 4758 Size: 8698 SalI G'TCGA_C Cuts at: 1936 6040 1936 Size: 4104 4594 SbfI CC_TGCA'GG Cuts at: 6050 6050 Size: 8698 ScaI AGT'ACT Cuts at: 3491 4046 4226 6946 8658 3491 Size: 555 180 2720 1712 3531 Fragments arranged by size: 3531 2720 1712 555 180 SfcI C'TryA_G Cuts at: 826 1045 2071 2353 4662 4691 4759 6046 7181 Size: 219 1026 282 2309 29 68 1287 1135 Cuts at: 7181 7862 8053 826 Size: 681 191 1471 Fragments arranged by size: 2309 1471 1287 1135 1026 681 282 219 191 68 29 SfoI GGC'GCC Cuts at: 5184 5184 Size: 8698 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8698 SmaI CCC'GGG Cuts at: 1872 6025 1872 Size: 4153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3526 4822 5381 5573 5584 6802 Size: 197 926 1583 1296 559 192 11 1218 Cuts at: 6802 7703 7965 820 Size: 901 262 1553 Fragments arranged by size: 1583 1553 1296 1218 926 901 559 262 197 192 11 SnaBI TAC'GTA Cuts at: 493 6489 493 Size: 5996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8698 SphI G_CATG'C Cuts at: 1132 2893 3568 6056 1132 Size: 1761 675 2488 3774 Fragments arranged by size: 3774 2488 1761 675 SspI AAT'ATT Cuts at: 5 52 5302 6622 5 Size: 47 5250 1320 2081 Fragments arranged by size: 5250 2081 1320 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8698 StyI C'CwwG_G Cuts at: 513 1065 2305 2715 3399 3556 3817 4008 4378 Size: 552 1240 410 684 157 261 191 370 Cuts at: 4378 4450 5178 6137 513 Size: 72 728 959 3074 Fragments arranged by size: 3074 1240 959 728 684 552 410 370 261 191 157 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2320 3287 4766 5267 6725 6742 6895 Size: 1255 740 967 1479 501 1458 17 153 Cuts at: 6895 7080 7525 325 Size: 185 445 1498 Fragments arranged by size: 1498 1479 1458 1255 967 740 501 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3489 4044 4224 Size: 274 80 33 51 1443 1512 555 180 Cuts at: 4224 6944 8656 96 Size: 2720 1712 138 Fragments arranged by size: 2720 1712 1512 1443 555 274 180 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3896 3928 4949 4981 8658 8690 3896 Size: 32 1021 32 3677 32 3904 Fragments arranged by size: 3904 3677 1021 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8698 XbaI T'CTAG_A Cuts at: 6034 6034 Size: 8698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3229 4316 4693 5224 3229 Size: 1087 377 531 6703 Fragments arranged by size: 6703 1087 531 377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8698 XmaI C'CCGG_G Cuts at: 1870 6023 1870 Size: 4153 4545 XmnI GAAnn'nnTTC Cuts at: 6827 6827 Size: 8698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2658 6506 276 Size: 53 83 186 936 1124 3848 2468 Fragments arranged by size: 3848 2468 1124 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FseI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI