(Circular) (Six-base) MAPSORT of: pFN21AA0810.seq Check: 4584 from: 1 to: 7141 pFN21AA0810 7141 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3178 3178 Size: 7141 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4951 278 Size: 53 83 186 936 3415 2468 Fragments arranged by size: 3415 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4397 4484 1937 Size: 2460 87 4594 Fragments arranged by size: 4594 2460 87 Acc65I G'GTAC_C Cuts at: 2886 4113 4462 2886 Size: 1227 349 5565 Fragments arranged by size: 5565 1227 349 AclI AA'CG_TT Cuts at: 1563 5268 5641 1563 Size: 3705 373 3063 Fragments arranged by size: 3705 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2079 2273 3143 5204 6588 6856 1632 Size: 447 194 870 2061 1384 268 1917 Fragments arranged by size: 2061 1917 1384 870 447 268 194 AfeI AGC'GCT Cuts at: 2242 2242 Size: 7141 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6944 AflIII A'CryG_T Cuts at: 1203 2025 2067 2322 3096 3565 6040 7017 1203 Size: 822 42 255 774 469 2475 977 1327 Fragments arranged by size: 2475 1327 977 822 774 469 255 42 AgeI A'CCGG_T Cuts at: 5972 5972 Size: 7141 AhdI GACnn_n'nnGTC Cuts at: 5870 5870 Size: 7141 AleI CACnn'nnGTG Cuts at: 1166 3222 1166 Size: 2056 5085 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2609 3888 1184 Size: 1425 1279 4437 Fragments arranged by size: 4437 1425 1279 AlwNI CAG_nnn'CTG Cuts at: 2565 2764 2855 3133 3262 3499 3685 3760 4270 Size: 199 91 278 129 237 186 75 510 Cuts at: 4270 4324 4884 6456 2565 Size: 54 560 1572 3250 Fragments arranged by size: 3250 1572 560 510 278 237 199 186 129 91 75 54 ApaI G_GGCC'C Cuts at: 2625 2625 Size: 7141 ApaLI G'TGCA_C Cuts at: 5198 6354 5198 Size: 1156 5985 ApoI r'AATT_y Cuts at: 1493 4450 4676 4828 6937 1493 Size: 2957 226 152 2109 1697 Fragments arranged by size: 2957 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5695 160 Size: 872 4663 1606 Fragments arranged by size: 4663 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 AvaI C'yCGr_G Cuts at: 1870 1943 2618 3270 3340 4466 1870 Size: 73 675 652 70 1126 4545 Fragments arranged by size: 4545 1126 675 652 73 70 BamHI G'GATC_C Cuts at: 4020 4471 4838 4020 Size: 451 367 6323 Fragments arranged by size: 6323 451 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2219 2231 2685 2886 2937 Size: 325 217 627 432 12 454 201 51 Cuts at: 2937 4113 4462 5917 618 Size: 1176 349 1455 1842 Fragments arranged by size: 1842 1455 1176 627 454 432 349 325 217 201 51 12 BanII G_rGCy'C Cuts at: 1391 2625 4460 1391 Size: 1234 1835 4072 Fragments arranged by size: 4072 1835 1234 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3578 928 Size: 971 1679 4491 Fragments arranged by size: 4491 1679 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2687 2721 2934 2968 5332 5366 1903 Size: 34 750 34 213 34 2364 34 3678 Fragments arranged by size: 3678 2364 750 213 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5033 6243 5033 Size: 1210 5931 BclI T'GATC_A Cuts at: 1548 4288 1548 Size: 2740 4401 BfrBI ATG'CAT Cuts at: 3064 3064 Size: 7141 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5752 136 Size: 107 122 71 631 605 4080 1525 Fragments arranged by size: 4080 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7136 7136 Size: 7141 BlpI GC'TnA_GC Cuts at: 4558 4558 Size: 7141 Bme1580I G_kGCm'C Cuts at: 1790 2625 5202 6358 1790 Size: 835 2577 1156 2573 Fragments arranged by size: 2577 2573 1156 835 BmgBI CAC'GTC Cuts at: 1318 2340 2593 3097 3616 1318 Size: 1022 253 504 519 4843 Fragments arranged by size: 4843 1022 519 504 253 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2035 3061 4390 Size: 416 53 83 399 24 618 1026 1329 Cuts at: 4390 4872 5830 442 Size: 482 958 1753 Fragments arranged by size: 1753 1329 1026 958 618 482 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7141 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2823 2877 3503 3545 4184 4292 5801 1239 Size: 1584 54 626 42 639 108 1509 2579 Fragments arranged by size: 2579 1584 1509 639 626 108 54 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 5266 6131 6429 3982 Size: 1284 865 298 4694 Fragments arranged by size: 4694 1284 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3005 3735 5804 882 Size: 617 1506 730 2069 2219 Fragments arranged by size: 2219 2069 1506 730 617 BsaAI yAC'GTr Cuts at: 493 1204 2070 2323 3568 3872 4284 4932 493 Size: 711 866 253 1245 304 412 648 2702 Fragments arranged by size: 2702 1245 866 711 648 412 304 253 BsaBI GATnn'nnATC Cuts at: 2472 7135 2472 Size: 4663 2478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2349 3845 4948 5330 Size: 53 83 186 936 816 1496 1103 382 Cuts at: 5330 5965 275 Size: 635 1451 Fragments arranged by size: 1496 1451 1103 936 816 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5574 5972 6246 6393 6804 5574 Size: 398 274 147 411 5911 Fragments arranged by size: 5911 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 1178 Size: 30 165 30 1134 30 5752 Fragments arranged by size: 5752 1134 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7141 BseYI C'CCAG_C Cuts at: 2872 4089 4147 6344 6840 2872 Size: 1217 58 2197 496 3173 Fragments arranged by size: 3173 2197 1217 496 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2760 1163 Size: 573 1024 5544 Fragments arranged by size: 5544 1024 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2707 4591 5352 6380 1540 Size: 275 186 706 1884 761 1028 2301 Fragments arranged by size: 2301 1884 1028 761 706 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 2598 3516 4170 5202 5287 6358 2124 Size: 474 918 654 1032 85 1071 2907 Fragments arranged by size: 2907 1071 1032 918 654 474 85 BsmI GAATG_Cn' Cuts at: 4652 4745 4652 Size: 93 7048 BsmBI CGTCTCn'nnnn_ Cuts at: 2108 3315 2108 Size: 1207 5934 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2598 2625 3516 4170 4460 5202 Size: 399 334 474 27 891 654 290 742 Cuts at: 5202 5287 6358 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 891 742 654 474 399 334 290 85 27 BspEI T'CCGG_A Cuts at: 6804 6804 Size: 7141 BspHI T'CATG_A Cuts at: 5030 6760 5030 Size: 1730 5411 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2323 2732 2987 3178 3753 4496 844 Size: 1479 409 255 191 575 743 3489 Fragments arranged by size: 3489 1479 743 575 409 255 191 BsrBI CCG'CTC Cuts at: 1394 1823 2264 5028 1394 Size: 429 441 2764 3507 Fragments arranged by size: 3507 2764 441 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5636 5810 65 Size: 1183 4388 174 1396 Fragments arranged by size: 4388 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3575 5785 5972 1700 Size: 110 1765 2210 187 2869 Fragments arranged by size: 2869 2210 1765 187 110 BsrGI T'GTAC_A Cuts at: 96 2028 96 Size: 1932 5209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7141 BssSI C'ACGA_G Cuts at: 5201 6213 5201 Size: 1012 6129 BstAPI GCAn_nnn'nTGC Cuts at: 794 2565 2764 3133 3499 3733 3760 794 Size: 1771 199 369 366 234 27 4175 Fragments arranged by size: 4175 1771 369 366 234 199 27 BstBI TT'CG_AA Cuts at: 2944 5945 2944 Size: 3001 4140 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7141 BstXI CCAn_nnnn'nTGG Cuts at: 3221 3221 Size: 7141 BstYI r'GATC_y Cuts at: 1972 3756 4020 4471 4838 5223 5240 6692 6778 Size: 1784 264 451 367 385 17 1452 86 Cuts at: 6778 6790 7136 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1784 1452 451 385 367 346 264 86 17 12 BstZ17I GTA'TAC Cuts at: 4398 4398 Size: 7141 BtgI C'CryG_G Cuts at: 513 1065 2004 2150 2610 2829 3158 3220 3816 Size: 552 939 146 460 219 329 62 596 Cuts at: 3816 513 Size: 3838 Fragments arranged by size: 3838 939 596 552 460 329 219 146 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6402 Fragments arranged by size: 6402 683 56 BtsI GCAGTG_nn' Cuts at: 4657 5451 5471 4657 Size: 794 20 6327 Fragments arranged by size: 6327 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7141 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7106 DraI TTT'AAA Cuts at: 4099 4445 4099 Size: 346 6795 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2809 3620 4226 6148 809 Size: 507 1493 811 606 1922 1802 Fragments arranged by size: 1922 1802 1493 811 606 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2265 2826 Size: 54 1425 50 275 18 348 87 561 Cuts at: 2826 4588 5477 7106 8 Size: 1762 889 1629 43 Fragments arranged by size: 1762 1629 1425 889 561 348 275 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4588 1537 Size: 275 2776 4090 Fragments arranged by size: 4090 2776 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2987 5071 1353 Size: 1634 2084 3423 Fragments arranged by size: 3423 2084 1634 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5728 6102 6248 387 Size: 1064 138 4139 374 146 1280 Fragments arranged by size: 4139 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2273 2823 2877 3143 3503 3545 Size: 393 447 194 550 54 266 360 42 Cuts at: 3545 4184 4292 5204 5801 6588 6856 1239 Size: 639 108 912 597 787 268 1524 Fragments arranged by size: 1524 912 787 639 597 550 447 393 360 268 266 194 108 54 42 EcoO109I rG'GnC_Cy Cuts at: 2715 4321 2715 Size: 1606 5535 EcoRI G'AATT_C Cuts at: 4450 4828 4450 Size: 378 6763 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4283 4283 Size: 7141 FspI TGC'GCA Cuts at: 2768 5647 2768 Size: 2879 4262 HaeII r_GCGC'y Cuts at: 1678 2244 3297 3891 4004 4212 6288 1678 Size: 566 1053 594 113 208 2076 2531 Fragments arranged by size: 2531 2076 1053 594 566 208 113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4286 4318 5782 5814 5856 Size: 32 135 32 2714 32 1464 32 42 Cuts at: 5856 5888 1373 Size: 32 2626 Fragments arranged by size: 2714 2626 1464 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4485 4731 669 Size: 988 281 2547 246 3079 Fragments arranged by size: 3079 2547 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7141 HpaI GTT'AAC Cuts at: 4731 4731 Size: 7141 KpnI G_GTAC'C Cuts at: 2890 4117 4466 2890 Size: 1227 349 5565 Fragments arranged by size: 5565 1227 349 MfeI C'AATT_G Cuts at: 4740 4740 Size: 7141 MluI A'CGCG_T Cuts at: 7017 7017 Size: 7141 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6255 6439 1305 Size: 4950 184 2007 Fragments arranged by size: 4950 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2180 10 Size: 54 1425 343 348 4971 Fragments arranged by size: 4971 1425 348 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3219 3222 5099 5458 5617 518 Size: 648 2053 3 1877 359 159 2042 Fragments arranged by size: 2053 2042 1877 648 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2229 2612 3076 3523 3558 3682 3751 4027 Size: 623 383 464 447 35 124 69 276 Cuts at: 4027 4557 5234 6382 6627 1606 Size: 530 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 623 530 464 447 383 276 245 124 69 35 NcoI C'CATG_G Cuts at: 513 1065 2004 3220 513 Size: 552 939 1216 4434 Fragments arranged by size: 4434 1216 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7141 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7141 NotI GC'GGCC_GC Cuts at: 4588 4588 Size: 7141 NsiI A_TGCA'T Cuts at: 3066 3066 Size: 7141 NspI r_CATG'y Cuts at: 1132 1229 2029 2193 2575 2739 3064 4499 6044 Size: 97 800 164 382 164 325 1435 1545 Cuts at: 6044 1132 Size: 2229 Fragments arranged by size: 2229 1545 1435 800 382 325 164 164 97 PasI CC'CwG_GG Cuts at: 1348 2197 4108 1348 Size: 849 1911 4381 Fragments arranged by size: 4381 1911 849 PciI A'CATG_T Cuts at: 2025 6040 2025 Size: 4015 3126 PflMI CCAn_nnn'nTGG Cuts at: 1108 4357 1108 Size: 3249 3892 PfoI T'CCnGG_A Cuts at: 2798 2798 Size: 7141 PmeI GTTT'AAAC Cuts at: 4445 4445 Size: 7141 PmlI CAC'GTG Cuts at: 2323 3568 3872 2323 Size: 1245 304 5592 Fragments arranged by size: 5592 1245 304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5210 5242 6723 6755 5210 Size: 32 1481 32 5596 Fragments arranged by size: 5596 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2715 2715 Size: 7141 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7141 PsiI TTA'TAA Cuts at: 4711 4711 Size: 7141 PspOMI G'GGCC_C Cuts at: 2621 2621 Size: 7141 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2048 2080 3958 3990 2048 Size: 32 1878 32 5199 Fragments arranged by size: 5199 1878 32 32 PstI C_TGCA'G Cuts at: 830 2379 2561 2570 2760 3138 3267 3690 3750 Size: 1549 182 9 190 378 129 423 60 Cuts at: 3750 3756 4493 830 Size: 6 737 3478 Fragments arranged by size: 3478 1549 737 423 378 190 182 129 60 9 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7141 PvuII CAG'CTG Cuts at: 3523 3558 3682 3751 3523 Size: 35 124 69 6913 Fragments arranged by size: 6913 124 69 35 SacII CC_GC'GG Cuts at: 2613 2613 Size: 7141 SalI G'TCGA_C Cuts at: 1936 4483 1936 Size: 2547 4594 SanDI GG'GwC_CC Cuts at: 2715 2715 Size: 7141 SbfI CC_TGCA'GG Cuts at: 2379 4493 2379 Size: 2114 5027 ScaI AGT'ACT Cuts at: 3967 5389 7101 3967 Size: 1422 1712 4007 Fragments arranged by size: 4007 1712 1422 SexAI A'CCwGG_T Cuts at: 2933 4116 2933 Size: 1183 5958 SfcI C'TryA_G Cuts at: 826 1045 2375 2557 2566 2756 3134 3254 3263 Size: 219 1330 182 9 190 378 120 9 Cuts at: 3263 3686 3746 3752 4489 5624 6305 6496 826 Size: 423 60 6 737 1135 681 191 1471 Fragments arranged by size: 1471 1330 1135 737 681 423 378 219 191 190 182 120 60 9 9 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7141 SmaI CCC'GGG Cuts at: 1872 2620 4468 1872 Size: 748 1848 4545 Fragments arranged by size: 4545 1848 748 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 5245 6146 6408 820 Size: 197 926 2018 1284 901 262 1553 Fragments arranged by size: 2018 1553 1284 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4932 493 Size: 4439 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7141 SphI G_CATG'C Cuts at: 1132 2193 2575 3064 4499 1132 Size: 1061 382 489 1435 3774 Fragments arranged by size: 3774 1435 1061 489 382 SspI AAT'ATT Cuts at: 5 52 5065 5 Size: 47 5013 2081 Fragments arranged by size: 5013 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7141 StyI C'CwwG_G Cuts at: 513 1065 2004 3220 4215 4580 513 Size: 552 939 1216 995 365 3074 Fragments arranged by size: 3074 1216 995 939 552 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2207 3304 3788 4161 5168 5185 5338 Size: 1255 627 1097 484 373 1007 17 153 Cuts at: 5338 5523 5968 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1097 1007 627 484 445 373 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2028 3965 5387 Size: 274 80 33 51 1443 51 1937 1422 Cuts at: 5387 7099 96 Size: 1712 138 Fragments arranged by size: 1937 1712 1443 1422 274 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3767 3799 7101 7133 3767 Size: 32 3302 32 3775 Fragments arranged by size: 3775 3302 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4183 1316 Size: 2867 4274 XbaI T'CTAG_A Cuts at: 4477 4477 Size: 7141 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7141 XmaI C'CCGG_G Cuts at: 1870 2618 4466 1870 Size: 748 1848 4545 Fragments arranged by size: 4545 1848 748 XmnI GAAnn'nnTTC Cuts at: 3405 5270 3405 Size: 1865 5276 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4949 276 Size: 53 83 186 936 3415 2468 Fragments arranged by size: 3415 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI RsrII SacI SapI SfiI SfoI SrfI SwaI XcmI