(Circular) (Six-base) MAPSORT of: pFN21AA0790.seq Check: 5114 from: 1 to: 8446 pFN21AA0790 8446 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5427 6256 278 Size: 53 83 186 936 3891 829 2468 Fragments arranged by size: 3891 2468 936 829 186 83 53 AccI GT'mk_AC Cuts at: 1937 4668 5789 1937 Size: 2731 1121 4594 Fragments arranged by size: 4594 2731 1121 Acc65I G'GTAC_C Cuts at: 5767 5767 Size: 8446 AclI AA'CG_TT Cuts at: 1563 2708 6573 6946 1563 Size: 1145 3865 373 3063 Fragments arranged by size: 3865 3063 1145 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2303 2883 3049 4358 4623 5334 5394 5596 Size: 671 580 166 1309 265 711 60 202 Cuts at: 5596 5639 6509 7893 8161 1632 Size: 43 870 1384 268 1917 Fragments arranged by size: 1917 1384 1309 870 711 671 580 268 265 202 166 60 43 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8249 AflIII A'CryG_T Cuts at: 1203 2232 2880 4386 7345 8322 1203 Size: 1029 648 1506 2959 977 1327 Fragments arranged by size: 2959 1506 1327 1029 977 648 AgeI A'CCGG_T Cuts at: 7277 7277 Size: 8446 AhdI GACnn_n'nnGTC Cuts at: 7175 7175 Size: 8446 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8446 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2217 2682 4677 1184 Size: 1033 465 1995 4953 Fragments arranged by size: 4953 1995 1033 465 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4873 4905 4873 Size: 32 8414 AlwNI CAG_nnn'CTG Cuts at: 2281 4153 4648 4757 5735 6189 7761 2281 Size: 1872 495 109 978 454 1572 2966 Fragments arranged by size: 2966 1872 1572 978 495 454 109 ApaI G_GGCC'C Cuts at: 4487 5024 5035 5240 4487 Size: 537 11 205 7693 Fragments arranged by size: 7693 537 205 11 ApaLI G'TGCA_C Cuts at: 2933 3686 6503 7659 2933 Size: 753 2817 1156 3720 Fragments arranged by size: 3720 2817 1156 753 ApoI r'AATT_y Cuts at: 1493 2890 4941 5755 5981 6133 8242 1493 Size: 1397 2051 814 226 152 2109 1697 Fragments arranged by size: 2109 2051 1697 1397 814 226 152 AseI AT'TA_AT Cuts at: 160 1032 7000 160 Size: 872 5968 1606 Fragments arranged by size: 5968 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8446 AvaI C'yCGr_G Cuts at: 1870 1943 2029 2048 2054 2066 3194 3436 4170 Size: 73 86 19 6 12 1128 242 734 Cuts at: 4170 5198 5268 5414 5771 1870 Size: 1028 70 146 357 4545 Fragments arranged by size: 4545 1128 1028 734 357 242 146 86 73 70 19 12 6 BamHI G'GATC_C Cuts at: 4118 5776 6143 4118 Size: 1658 367 6421 Fragments arranged by size: 6421 1658 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4898 5146 5591 5767 7222 Size: 325 217 627 3111 248 445 176 1455 Cuts at: 7222 618 Size: 1842 Fragments arranged by size: 3111 1842 1455 627 445 325 248 217 176 BanII G_rGCy'C Cuts at: 1391 2049 2055 2067 2073 2097 3563 4263 4487 Size: 658 6 12 6 24 1466 700 224 Cuts at: 4487 4745 5024 5035 5196 5240 5765 1391 Size: 258 279 11 161 44 525 4072 Fragments arranged by size: 4072 1466 700 658 525 279 258 224 161 44 24 12 11 6 6 BbeI G_GCGC'C Cuts at: 5150 5150 Size: 8446 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2424 2529 3527 3855 4023 4622 928 Size: 971 525 105 998 328 168 599 4752 Fragments arranged by size: 4752 998 971 599 525 328 168 105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6637 6671 1903 Size: 34 4700 34 3678 Fragments arranged by size: 4700 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2165 6338 7548 2165 Size: 4173 1210 3063 Fragments arranged by size: 4173 3063 1210 BclI T'GATC_A Cuts at: 1548 2604 1548 Size: 1056 7390 BfrBI ATG'CAT Cuts at: 4676 4676 Size: 8446 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5182 7057 136 Size: 107 122 71 631 605 3510 1875 1525 Fragments arranged by size: 3510 1875 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8441 8441 Size: 8446 BlpI GC'TnA_GC Cuts at: 4551 4806 4920 5863 4551 Size: 255 114 943 7134 Fragments arranged by size: 7134 943 255 114 Bme1580I G_kGCm'C Cuts at: 1790 2937 3690 4487 4901 5024 5035 5240 5269 Size: 1147 753 797 414 123 11 205 29 Cuts at: 5269 6507 7663 1790 Size: 1238 1156 2573 Fragments arranged by size: 2573 1238 1156 1147 797 753 414 205 123 29 11 BmgBI CAC'GTC Cuts at: 1318 2146 2233 2313 3006 3838 4389 4561 1318 Size: 828 87 80 693 832 551 172 5203 Fragments arranged by size: 5203 832 828 693 551 172 87 80 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2180 3699 4442 Size: 416 53 83 399 24 763 1519 743 Cuts at: 4442 5019 5034 6177 7135 442 Size: 577 15 1143 958 1753 Fragments arranged by size: 1753 1519 1143 958 763 743 577 416 399 83 53 24 15 BmtI G_CTAG'C Cuts at: 1056 2307 4233 1056 Size: 1251 1926 5269 Fragments arranged by size: 5269 1926 1251 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2143 3451 4079 4675 2143 Size: 1308 628 596 5914 Fragments arranged by size: 5914 1308 628 596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3001 4028 4499 4670 4757 4836 4868 5198 Size: 1762 1027 471 171 87 79 32 330 Cuts at: 5198 7106 1239 Size: 1908 2579 Fragments arranged by size: 2579 1908 1762 1027 471 330 171 87 79 32 Bpu10I CC'TnA_GC Cuts at: 2043 3203 2043 Size: 1160 7286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2618 3494 3713 3719 6571 7436 7734 2618 Size: 876 219 6 2852 865 298 3330 Fragments arranged by size: 3330 2852 876 865 298 219 6 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3197 4466 5263 7109 882 Size: 617 1698 1269 797 1846 2219 Fragments arranged by size: 2219 1846 1698 1269 797 617 BsaAI yAC'GTr Cuts at: 493 1204 2881 3832 6237 493 Size: 711 1677 951 2405 2702 Fragments arranged by size: 2702 2405 1677 951 711 BsaBI GATnn'nnATC Cuts at: 2153 8440 2153 Size: 6287 2159 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3883 4841 5147 5424 Size: 53 83 186 936 2350 958 306 277 Cuts at: 5424 6253 6635 7270 275 Size: 829 382 635 1451 Fragments arranged by size: 2350 1451 958 936 829 635 382 306 277 186 83 53 BsaWI w'CCGG_w Cuts at: 2089 6879 7277 7551 7698 8109 2089 Size: 4790 398 274 147 411 2426 Fragments arranged by size: 4790 2426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2511 2541 2867 2897 2992 Size: 30 165 30 1108 30 326 30 95 Cuts at: 2992 3022 3291 3321 5324 5354 1178 Size: 30 269 30 2003 30 4270 Fragments arranged by size: 4270 2003 1108 326 269 165 95 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2264 2579 2777 2888 3869 4025 4367 5348 Size: 1078 315 198 111 981 156 342 981 Cuts at: 5348 5690 1186 Size: 342 3942 Fragments arranged by size: 3942 1078 981 981 342 342 315 198 156 111 BseYI C'CCAG_C Cuts at: 2649 2855 3903 4262 4363 4641 5113 5233 7649 Size: 206 1048 359 101 278 472 120 2416 Cuts at: 7649 8145 2649 Size: 496 2950 Fragments arranged by size: 2950 2416 1048 496 472 359 278 206 120 101 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3242 5291 5405 1163 Size: 573 1506 2049 114 4204 Fragments arranged by size: 4204 2049 1506 573 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4623 5896 6657 7685 1540 Size: 275 186 2622 1273 761 1028 2301 Fragments arranged by size: 2622 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2880 2937 3563 3587 3690 3843 3954 5196 5223 Size: 57 626 24 103 153 111 1242 27 Cuts at: 5223 6507 6592 7663 2880 Size: 1284 85 1071 3663 Fragments arranged by size: 3663 1284 1242 1071 626 153 111 103 85 57 27 24 BsmI GAATG_Cn' Cuts at: 3456 4935 5957 6050 3456 Size: 1479 1022 93 5852 Fragments arranged by size: 5852 1479 1022 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3012 3374 5530 3012 Size: 362 2156 5928 Fragments arranged by size: 5928 2156 362 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2055 2067 2073 2097 2880 2937 Size: 399 259 6 12 6 24 783 57 Cuts at: 2937 3563 3587 3690 3843 3954 4263 4487 4745 Size: 626 24 103 153 111 309 224 258 Cuts at: 4745 4901 5024 5035 5196 5223 5240 5269 5765 Size: 156 123 11 161 27 17 29 496 Cuts at: 5765 6507 6592 7663 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 626 496 399 309 259 258 224 161 156 153 123 111 103 85 57 29 27 24 24 17 12 11 6 6 BspEI T'CCGG_A Cuts at: 8109 8109 Size: 8446 BspHI T'CATG_A Cuts at: 6335 8065 6335 Size: 1730 6716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3227 5801 844 Size: 2383 2574 3489 Fragments arranged by size: 3489 2574 2383 BsrBI CCG'CTC Cuts at: 1394 1823 2569 3162 3570 5419 6333 1394 Size: 429 746 593 408 1849 914 3507 Fragments arranged by size: 3507 1849 914 746 593 429 408 BsrDI GCAATG_nn' Cuts at: 65 1248 4307 4676 6941 7115 65 Size: 1183 3059 369 2265 174 1396 Fragments arranged by size: 3059 2265 1396 1183 369 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2655 2896 5588 5663 7090 7277 1700 Size: 110 845 241 2692 75 1427 187 2869 Fragments arranged by size: 2869 2692 1427 845 241 187 110 75 BsrGI T'GTAC_A Cuts at: 96 3309 96 Size: 3213 5233 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8446 BssSI C'ACGA_G Cuts at: 4868 6506 7518 4868 Size: 1638 1012 5796 Fragments arranged by size: 5796 1638 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4561 4930 794 Size: 3767 369 4310 Fragments arranged by size: 4310 3767 369 BstBI TT'CG_AA Cuts at: 7250 7250 Size: 8446 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8446 BstXI CCAn_nnnn'nTGG Cuts at: 3025 3109 3916 3025 Size: 84 807 7555 Fragments arranged by size: 7555 807 84 BstYI r'GATC_y Cuts at: 1972 3922 4118 5278 5445 5776 6143 6528 6545 Size: 1950 196 1160 167 331 367 385 17 Cuts at: 6545 7997 8083 8095 8441 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1950 1452 1160 385 367 346 331 196 167 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5736 5736 Size: 8446 BtgI C'CryG_G Cuts at: 513 1065 2004 3208 3774 5016 5604 5742 513 Size: 552 939 1204 566 1242 588 138 3217 Fragments arranged by size: 3217 1242 1204 939 588 566 552 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2574 487 Size: 683 56 1348 6359 Fragments arranged by size: 6359 1348 683 56 BtsI GCAGTG_nn' Cuts at: 4080 4201 5962 6756 6776 4080 Size: 121 1761 794 20 5750 Fragments arranged by size: 5750 1761 794 121 20 ClaI AT'CG_AT Cuts at: 1554 2181 3753 5322 1554 Size: 627 1572 1569 4678 Fragments arranged by size: 4678 1572 1569 627 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8411 DraI TTT'AAA Cuts at: 5750 5750 Size: 8446 DraIII CAC_nnn'GTG Cuts at: 4412 5227 4412 Size: 815 7631 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2764 4664 7453 809 Size: 507 1448 1900 2789 1802 Fragments arranged by size: 2789 1900 1802 1448 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2687 3043 5356 Size: 54 1425 50 275 18 857 356 2313 Cuts at: 5356 5893 6782 8411 8 Size: 537 889 1629 43 Fragments arranged by size: 2313 1629 1425 889 857 537 356 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5893 1537 Size: 275 4081 4090 Fragments arranged by size: 4090 4081 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2334 2403 3639 4633 5720 6376 1353 Size: 981 69 1236 994 1087 656 3423 Fragments arranged by size: 3423 1236 1087 994 981 656 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2905 3456 7033 7407 7553 387 Size: 1064 138 1316 551 3577 374 146 1280 Fragments arranged by size: 3577 1316 1280 1064 551 374 146 138 EcoICRI GAG'CTC Cuts at: 3561 5194 3561 Size: 1633 6813 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2883 3001 3049 4028 4358 4499 Size: 393 671 580 118 48 979 330 141 Cuts at: 4499 4623 4670 4757 4836 4868 5198 5334 5394 Size: 124 47 87 79 32 330 136 60 Cuts at: 5394 5596 5639 6509 7106 7893 8161 1239 Size: 202 43 870 597 787 268 1524 Fragments arranged by size: 1524 979 870 787 671 597 580 393 330 330 268 202 141 136 124 118 87 79 60 48 47 43 32 EcoNI CCTnn'n_nnAGG Cuts at: 3170 3657 5136 5657 3170 Size: 487 1479 521 5959 Fragments arranged by size: 5959 1479 521 487 EcoO109I rG'GnC_Cy Cuts at: 2278 3085 3198 3780 4042 4150 4483 4484 5021 Size: 807 113 582 262 108 333 1 537 Cuts at: 5021 5031 5032 5732 2278 Size: 10 1 700 4992 Fragments arranged by size: 4992 807 700 582 537 333 262 113 108 10 1 1 EcoRI G'AATT_C Cuts at: 2890 5755 6133 2890 Size: 2865 378 5203 Fragments arranged by size: 5203 2865 378 EcoRV GAT'ATC Cuts at: 3748 3748 Size: 8446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2363 2363 Size: 8446 FspI TGC'GCA Cuts at: 6952 6952 Size: 8446 HaeII r_GCGC'y Cuts at: 1678 2364 5150 7593 1678 Size: 686 2786 2443 2531 Fragments arranged by size: 2786 2531 2443 686 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2705 2737 2897 2929 2978 Size: 32 135 32 1133 32 160 32 49 Cuts at: 2978 3010 3707 3735 3739 3767 7087 7119 7161 Size: 32 697 28 4 28 3320 32 42 Cuts at: 7161 7193 1373 Size: 32 2626 Fragments arranged by size: 3320 2626 1133 697 160 135 49 42 32 32 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2370 4141 4669 5790 6036 669 Size: 988 281 432 1771 528 1121 246 3079 Fragments arranged by size: 3079 1771 1121 988 528 432 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8446 HpaI GTT'AAC Cuts at: 6036 6036 Size: 8446 KasI G'GCGC_C Cuts at: 5146 5146 Size: 8446 KpnI G_GTAC'C Cuts at: 5771 5771 Size: 8446 MfeI C'AATT_G Cuts at: 2610 6045 2610 Size: 3435 5011 MluI A'CGCG_T Cuts at: 8322 8322 Size: 8446 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2253 3606 3866 7560 7744 1305 Size: 948 1353 260 3694 184 2007 Fragments arranged by size: 3694 2007 1353 948 260 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2689 10 Size: 54 1425 343 857 5767 Fragments arranged by size: 5767 1425 857 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3830 6404 6763 6922 518 Size: 648 2664 2574 359 159 2042 Fragments arranged by size: 2664 2574 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2024 2323 5361 5606 5862 6539 7687 7932 Size: 418 299 3038 245 256 677 1148 245 Cuts at: 7932 1606 Size: 2120 Fragments arranged by size: 3038 2120 1148 677 418 299 256 245 245 NaeI GCC'GGC Cuts at: 5590 5665 5590 Size: 75 8371 NarI GG'CG_CC Cuts at: 5147 5147 Size: 8446 NcoI C'CATG_G Cuts at: 513 1065 2004 3774 5016 5742 513 Size: 552 939 1770 1242 726 3217 Fragments arranged by size: 3217 1770 1242 939 726 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8446 NgoMIV G'CCGG_C Cuts at: 5588 5663 5588 Size: 75 8371 NheI G'CTAG_C Cuts at: 1052 2303 4229 1052 Size: 1251 1926 5269 Fragments arranged by size: 5269 1926 1251 NotI GC'GGCC_GC Cuts at: 5893 5893 Size: 8446 NsiI A_TGCA'T Cuts at: 4678 4678 Size: 8446 NspI r_CATG'y Cuts at: 1132 1229 3958 5804 7349 1132 Size: 97 2729 1846 1545 2229 Fragments arranged by size: 2729 2229 1846 1545 97 PasI CC'CwG_GG Cuts at: 1348 4894 1348 Size: 3546 4900 PciI A'CATG_T Cuts at: 7345 7345 Size: 8446 PflMI CCAn_nnn'nTGG Cuts at: 1108 2281 4495 4522 4648 4894 5495 1108 Size: 1173 2214 27 126 246 601 4059 Fragments arranged by size: 4059 2214 1173 601 246 126 27 PfoI T'CCnGG_A Cuts at: 2157 2901 3927 4813 5214 2157 Size: 744 1026 886 401 5389 Fragments arranged by size: 5389 1026 886 744 401 PmeI GTTT'AAAC Cuts at: 5750 5750 Size: 8446 PmlI CAC'GTG Cuts at: 2881 2881 Size: 8446 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6515 6547 8028 8060 6515 Size: 32 1481 32 6901 Fragments arranged by size: 6901 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2278 3198 3780 4042 2278 Size: 920 582 262 6682 Fragments arranged by size: 6682 920 582 262 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8446 PsiI TTA'TAA Cuts at: 6016 6016 Size: 8446 PspOMI G'GGCC_C Cuts at: 4483 5020 5031 5236 4483 Size: 537 11 205 7693 Fragments arranged by size: 7693 537 205 11 PstI C_TGCA'G Cuts at: 830 3224 5707 5798 830 Size: 2394 2483 91 3478 Fragments arranged by size: 3478 2483 2394 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8446 PvuII CAG'CTG Cuts at: 2024 2323 2024 Size: 299 8147 RsrII CG'GwC_CG Cuts at: 2913 2913 Size: 8446 SacI G_AGCT'C Cuts at: 3563 5196 3563 Size: 1633 6813 SacII CC_GC'GG Cuts at: 5607 5607 Size: 8446 SalI G'TCGA_C Cuts at: 1936 4667 5788 1936 Size: 2731 1121 4594 Fragments arranged by size: 4594 2731 1121 SanDI GG'GwC_CC Cuts at: 3198 4042 3198 Size: 844 7602 SapI GCTCTTCn'nnn_ Cuts at: 3639 3639 Size: 8446 SbfI CC_TGCA'GG Cuts at: 5798 5798 Size: 8446 ScaI AGT'ACT Cuts at: 6694 8406 6694 Size: 1712 6734 SexAI A'CCwGG_T Cuts at: 4408 4408 Size: 8446 SfcI C'TryA_G Cuts at: 826 1045 2356 2427 3220 3525 5703 5794 6929 Size: 219 1311 71 793 305 2178 91 1135 Cuts at: 6929 7610 7801 826 Size: 681 191 1471 Fragments arranged by size: 2178 1471 1311 1135 793 681 305 219 191 91 71 SfoI GGC'GCC Cuts at: 5148 5148 Size: 8446 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8446 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8446 SmaI CCC'GGG Cuts at: 1872 2031 5270 5773 1872 Size: 159 3239 503 4545 Fragments arranged by size: 4545 3239 503 159 SmlI C'TyrA_G Cuts at: 820 1017 1943 2633 3509 3728 3734 6550 7451 Size: 197 926 690 876 219 6 2816 901 Cuts at: 7451 7713 820 Size: 262 1553 Fragments arranged by size: 2816 1553 926 901 876 690 262 219 197 6 SnaBI TAC'GTA Cuts at: 493 6237 493 Size: 5744 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8446 SphI G_CATG'C Cuts at: 1132 5804 1132 Size: 4672 3774 SspI AAT'ATT Cuts at: 5 52 6370 5 Size: 47 6318 2081 Fragments arranged by size: 6318 2081 47 StuI AGG'CCT Cuts at: 1517 5131 1517 Size: 3614 4832 StyI C'CwwG_G Cuts at: 513 1065 2004 3774 5016 5742 5885 513 Size: 552 939 1770 1242 726 143 3074 Fragments arranged by size: 3074 1770 1242 939 726 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2156 3787 4355 5523 5561 6473 6490 Size: 1255 576 1631 568 1168 38 912 17 Cuts at: 6490 6643 6828 7273 325 Size: 153 185 445 1498 Fragments arranged by size: 1631 1498 1255 1168 912 576 568 445 185 153 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3309 6692 8404 Size: 274 80 33 51 1443 1332 3383 1712 Cuts at: 8404 96 Size: 138 Fragments arranged by size: 3383 1712 1443 1332 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2992 3024 3291 3323 3691 3723 8406 8438 2992 Size: 32 267 32 368 32 4683 32 3000 Fragments arranged by size: 4683 3000 368 267 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8446 XbaI T'CTAG_A Cuts at: 5281 5782 5281 Size: 501 7945 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4154 4154 Size: 8446 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8446 XmaI C'CCGG_G Cuts at: 1870 2029 5268 5771 1870 Size: 159 3239 503 4545 Fragments arranged by size: 4545 3239 503 159 XmnI GAAnn'nnTTC Cuts at: 3534 6575 3534 Size: 3041 5405 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5425 6254 276 Size: 53 83 186 936 3891 829 2468 Fragments arranged by size: 3891 2468 936 829 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BsiWI BstZ17I FseI FspAI NruI PacI PspXI PsrI SfiI SrfI SwaI