Restriction map for
pFN21AA0783
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoT22I FseI HaeII Hin1I
KasI KpnI KspI MaeII MluI
MroI MslI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AocI 2512 CCTGAGG CC^T[ACGT]AGG
AseI 285 ATTAAT AT^TAAT
AsnI 285 ATTAAT AT^TAAT
AvaII 948 GGACC G^G[AT]CC
AviII 1883 TGCGCA TGC^GCA
BalI 2363 TGGCCA TGG^CCA
BamHI 2265 GGATCC G^GATCC
BanI 1309 GGTGCC G^G[CT][GA]CC
BanII 933 GAGCTC G[GA]GC[CT]^C
BclI 2205 TGATCA T^GATCA
BcnI 1616 CCCGG CC^[GC]GG
BglII 670 AGATCT A^GATCT
BsgI 1386 GTGCAG GTGCAG(16/14)
BspMI 24 GCAGGT ACCTGC(4/8)
BsrDI 761 GCAATG GCAATG(2/0)
BsrFI 2088 ACCGGC [GA]^CCGG[CT]
BssSI 2478 CACGAG CACGAG(-5/-1)
BstBI 2024 TTCGAA TT^CGAA
BstXI 538 CCAAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2512 CCTGAGG CC^T[ACGT]AGG
CfoI 1884 GCGC GCG^C
Cfr10I 2088 ACCGGC [GA]^CCGG[CT]
Cfr13I 948 GGACC G^G[ACGT]CC
DraI 91 TTTAAA TTT^AAA
EaeI 2363 TGGCCA [CT]^GGCC[GA]
Eco81I 2512 CCTGAGG CC^T[ACGT]AGG
EcoRV 1771 GATATC GAT^ATC
FauI 731 GCGGG CCCGC(4/6)
FbaI 2205 TGATCA T^GATCA
FspI 1883 TGCGCA TGC^GCA
HgaI 994 GACGC GACGC(5/10)
HhaI 1884 GCGC GCG^C
HinP1I 1884 GCGC G^CGC
HincII 478 GTTAAC GT[CT]^[GA]AC
HindII 478 GTTAAC GT[CT]^[GA]AC
HpaI 478 GTTAAC GTT^AAC
MfeI 1020 CAATTG C^AATTG
MluNI 2363 TGGCCA TGG^CCA
MscI 2363 TGGCCA TGG^CCA
MspA1I 1442 CAGCGG C[AC]G^C[GT]G
MunI 1020 CAATTG C^AATTG
NciI 1616 CCCGG CC^[GC]GG
NspI 1491 ACATGC [GA]CATG^[CT]
NspV 2024 TTCGAA TT^CGAA
PacI 286 TTAATTAA TTAAT^TAA
PshBI 285 ATTAAT AT^TAAT
SacI 933 GAGCTC GAGCT^C
SapI 592 GAAGAGC GCTCTTC(1/4)
Sau96I 948 GGACC G^G[ACGT]CC
SfuI 2024 TTCGAA TT^CGAA
SmlI 913 CTTGAG C^T[CT][GA]AG
SpeI 1798 ACTAGT A^CTAGT
SspI 190 AATATT AAT^ATT
StuI 1670 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 10 enzymes) #
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BseRI 2270 CTCCTC GAGGAG(10/8)
18 GAGGAG
Cac8I 533 GCGAGC GC[ACGT]^[ACGT]GC
2100 GCGAGC
DsaI 587 CCATGG C^C[GA][CT]GG
2366 CCATGG
HaeIII 1671 GGCC GG^CC
2364 GGCC
HapII 1617 CCGG C^CGG
2089 CCGG
HindIII 1957 AAGCTT A^AGCTT
2502 AAGCTT
HpaII 1617 CCGG C^CGG
2089 CCGG
MspI 1617 CCGG C^CGG
2089 CCGG
NcoI 587 CCATGG C^CATGG
2366 CCATGG
ScaI 264 AGTACT AGT^ACT
2169 AGTACT
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaBI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HinfI
HphI ItaI Ksp632I MaeI MaeIII
MamI MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI