(Circular) (Six-base) MAPSORT of: pFN21AA0783.seq Check: 940 from: 1 to: 7369 pFN21AA0783 7369 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2020 2020 Size: 7369 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5179 278 Size: 53 83 186 936 3643 2468 Fragments arranged by size: 3643 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4712 1937 Size: 2775 4594 Acc65I G'GTAC_C Cuts at: 4690 4690 Size: 7369 AclI AA'CG_TT Cuts at: 1563 5496 5869 1563 Size: 3933 373 3063 Fragments arranged by size: 3933 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2142 2208 3611 3708 4617 4650 5432 6816 Size: 510 66 1403 97 909 33 782 1384 Cuts at: 6816 7084 1632 Size: 268 1917 Fragments arranged by size: 1917 1403 1384 909 782 510 268 97 66 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7172 AflIII A'CryG_T Cuts at: 1203 6268 7245 1203 Size: 5065 977 1327 Fragments arranged by size: 5065 1327 977 AgeI A'CCGG_T Cuts at: 6200 6200 Size: 7369 AhdI GACnn_n'nnGTC Cuts at: 6098 6098 Size: 7369 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7369 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3406 3654 1184 Size: 2222 248 4899 Fragments arranged by size: 4899 2222 248 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3198 3230 3198 Size: 32 7337 AlwNI CAG_nnn'CTG Cuts at: 2120 2426 3098 3203 5112 6684 2120 Size: 306 672 105 1909 1572 2805 Fragments arranged by size: 2805 1909 1572 672 306 105 ApaLI G'TGCA_C Cuts at: 5426 6582 5426 Size: 1156 6213 ApoI r'AATT_y Cuts at: 1493 2251 3183 3900 4430 4678 4904 5056 7165 Size: 758 932 717 530 248 226 152 2109 Cuts at: 7165 1493 Size: 1697 Fragments arranged by size: 2109 1697 932 758 717 530 248 226 152 AseI AT'TA_AT Cuts at: 160 1032 2291 5923 160 Size: 872 1259 3632 1606 Fragments arranged by size: 3632 1606 1259 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7369 AvaI C'yCGr_G Cuts at: 1870 1943 4694 1870 Size: 73 2751 4545 Fragments arranged by size: 4545 2751 73 BamHI G'GATC_C Cuts at: 4270 4699 5066 4270 Size: 429 367 6573 Fragments arranged by size: 6573 429 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3314 4690 6145 618 Size: 325 217 627 1527 1376 1455 1842 Fragments arranged by size: 1842 1527 1455 1376 627 325 217 BanII G_rGCy'C Cuts at: 1391 2942 4688 1391 Size: 1551 1746 4072 Fragments arranged by size: 4072 1746 1551 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2754 3345 4564 928 Size: 971 855 591 1219 3733 Fragments arranged by size: 3733 1219 971 855 591 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5560 5594 1903 Size: 34 3623 34 3678 Fragments arranged by size: 3678 3623 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5261 6471 5261 Size: 1210 6159 BclI T'GATC_A Cuts at: 1548 4210 1548 Size: 2662 4707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5980 136 Size: 107 122 71 631 605 4308 1525 Fragments arranged by size: 4308 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2675 7364 2675 Size: 4689 2680 BlpI GC'TnA_GC Cuts at: 4786 4786 Size: 7369 Bme1580I G_kGCm'C Cuts at: 1790 3232 5430 6586 1790 Size: 1442 2198 1156 2573 Fragments arranged by size: 2573 2198 1442 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7369 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3369 5100 6058 Size: 416 53 83 399 24 1952 1731 958 Cuts at: 6058 442 Size: 1753 Fragments arranged by size: 1952 1753 1731 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7369 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3094 3094 Size: 7369 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2956 3264 4656 6029 1239 Size: 1717 308 1392 1373 2579 Fragments arranged by size: 2579 1717 1392 1373 308 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2939 5494 6359 6657 2939 Size: 2555 865 298 3651 Fragments arranged by size: 3651 2555 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6032 882 Size: 617 4533 2219 Fragments arranged by size: 4533 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5160 493 Size: 711 3956 2702 Fragments arranged by size: 3956 2702 711 BsaBI GATnn'nnATC Cuts at: 2680 2775 4215 7363 2680 Size: 95 1440 3148 2686 Fragments arranged by size: 3148 2686 1440 95 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5176 5558 6193 275 Size: 53 83 186 936 3643 382 635 1451 Fragments arranged by size: 3643 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5802 6200 6474 6621 7032 5802 Size: 398 274 147 411 6139 Fragments arranged by size: 6139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2458 2488 1178 Size: 30 165 30 1055 30 6059 Fragments arranged by size: 6059 1055 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 4266 1186 Size: 852 2228 4289 Fragments arranged by size: 4289 2228 852 BseYI C'CCAG_C Cuts at: 6572 7068 6572 Size: 496 6873 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3412 1163 Size: 573 1676 5120 Fragments arranged by size: 5120 1676 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4819 5580 6608 1540 Size: 275 186 2818 761 1028 2301 Fragments arranged by size: 2818 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2942 3421 4111 4325 5430 5515 6586 2942 Size: 479 690 214 1105 85 1071 3725 Fragments arranged by size: 3725 1105 1071 690 479 214 85 BsmI GAATG_Cn' Cuts at: 4880 4973 4880 Size: 93 7276 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2942 3232 3421 4111 4325 4688 5430 Size: 399 1152 290 189 690 214 363 742 Cuts at: 5430 5515 6586 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1152 1071 742 690 399 363 290 214 189 85 BspEI T'CCGG_A Cuts at: 7032 7032 Size: 7369 BspHI T'CATG_A Cuts at: 5258 6988 5258 Size: 1730 5639 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2020 4724 844 Size: 1176 2704 3489 Fragments arranged by size: 3489 2704 1176 BsrBI CCG'CTC Cuts at: 1394 1823 5256 1394 Size: 429 3433 3507 Fragments arranged by size: 3507 3433 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2773 5864 6038 65 Size: 1183 1525 3091 174 1396 Fragments arranged by size: 3091 1525 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4093 6013 6200 1700 Size: 110 2283 1920 187 2869 Fragments arranged by size: 2869 2283 1920 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7369 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7369 BssSI C'ACGA_G Cuts at: 4483 5429 6441 4483 Size: 946 1012 5411 Fragments arranged by size: 5411 1012 946 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7369 BstBI TT'CG_AA Cuts at: 4030 6173 4030 Size: 2143 5226 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7369 BstXI CCAn_nnnn'nTGG Cuts at: 2550 2550 Size: 7369 BstYI r'GATC_y Cuts at: 1972 2675 3469 4270 4699 5066 5451 5468 6920 Size: 703 794 801 429 367 385 17 1452 Cuts at: 6920 7006 7018 7364 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 801 794 703 429 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4518 4518 Size: 7369 BtgI C'CryG_G Cuts at: 513 1065 2004 2592 4371 513 Size: 552 939 588 1779 3511 Fragments arranged by size: 3511 1779 939 588 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6630 Fragments arranged by size: 6630 683 56 BtsI GCAGTG_nn' Cuts at: 3724 3849 4325 4354 4885 5679 5699 3724 Size: 125 476 29 531 794 20 5394 Fragments arranged by size: 5394 794 531 476 125 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7369 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7334 DraI TTT'AAA Cuts at: 2098 4673 2098 Size: 2575 4794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6376 809 Size: 507 5060 1802 Fragments arranged by size: 5060 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4368 4816 5705 Size: 54 1425 50 275 18 2538 448 889 Cuts at: 5705 7334 8 Size: 1629 43 Fragments arranged by size: 2538 1629 1425 889 448 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4816 1537 Size: 275 3004 4090 Fragments arranged by size: 4090 3004 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2592 2813 2918 3450 3518 4170 5299 1353 Size: 1239 221 105 532 68 652 1129 3423 Fragments arranged by size: 3423 1239 1129 652 532 221 105 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3467 5956 6330 6476 387 Size: 1064 138 1878 2489 374 146 1280 Fragments arranged by size: 2489 1878 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2940 2940 Size: 7369 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2208 2956 3264 3611 3708 4617 Size: 393 510 66 748 308 347 97 909 Cuts at: 4617 4650 4656 5432 6029 6816 7084 1239 Size: 33 6 776 597 787 268 1524 Fragments arranged by size: 1524 909 787 776 748 597 510 393 347 308 268 97 66 33 6 EcoRI G'AATT_C Cuts at: 4678 5056 4678 Size: 378 6991 EcoRV GAT'ATC Cuts at: 3778 3778 Size: 7369 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2261 3452 3980 2261 Size: 1191 528 5650 Fragments arranged by size: 5650 1191 528 FspI TGC'GCA Cuts at: 3890 5875 3890 Size: 1985 5384 FspAI rTGC'GCAy Cuts at: 3890 3890 Size: 7369 HaeII r_GCGC'y Cuts at: 1678 6516 1678 Size: 4838 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2573 2605 2869 Size: 32 135 32 428 32 541 32 264 Cuts at: 2869 2901 3403 3435 4147 4179 6010 6042 6084 Size: 32 502 32 712 32 1831 32 42 Cuts at: 6084 6116 1373 Size: 32 2626 Fragments arranged by size: 2626 1831 712 541 502 428 264 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2485 4713 4959 669 Size: 988 281 547 2228 246 3079 Fragments arranged by size: 3079 2228 988 547 281 246 HindIII A'AGCT_T Cuts at: 748 3962 4507 748 Size: 3214 545 3610 Fragments arranged by size: 3610 3214 545 HpaI GTT'AAC Cuts at: 2485 4959 2485 Size: 2474 4895 KpnI G_GTAC'C Cuts at: 4694 4694 Size: 7369 MfeI C'AATT_G Cuts at: 3025 4968 3025 Size: 1943 5426 MluI A'CGCG_T Cuts at: 7245 7245 Size: 7369 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2083 6483 6667 1305 Size: 778 4400 184 2007 Fragments arranged by size: 4400 2007 778 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4370 10 Size: 54 1425 343 2538 3009 Fragments arranged by size: 3009 2538 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5327 5686 5845 518 Size: 648 4161 359 159 2042 Fragments arranged by size: 4161 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3449 4785 5462 6610 6855 1606 Size: 1843 1336 677 1148 245 2120 Fragments arranged by size: 2120 1843 1336 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2592 4371 513 Size: 552 939 588 1779 3511 Fragments arranged by size: 3511 1779 939 588 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7369 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7369 NotI GC'GGCC_GC Cuts at: 4816 4816 Size: 7369 NspI r_CATG'y Cuts at: 1132 1229 3500 4727 6272 1132 Size: 97 2271 1227 1545 2229 Fragments arranged by size: 2271 2229 1545 1227 97 PacI TTA_AT'TAA Cuts at: 2295 2295 Size: 7369 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7369 PciI A'CATG_T Cuts at: 6268 6268 Size: 7369 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7369 PfoI T'CCnGG_A Cuts at: 3241 3241 Size: 7369 PmeI GTTT'AAAC Cuts at: 4673 4673 Size: 7369 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5438 5470 6951 6983 5438 Size: 32 1481 32 5824 Fragments arranged by size: 5824 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7369 PsiI TTA'TAA Cuts at: 4939 4939 Size: 7369 PstI C_TGCA'G Cuts at: 830 2260 3856 4174 4721 830 Size: 1430 1596 318 547 3478 Fragments arranged by size: 3478 1596 1430 547 318 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7369 SacI G_AGCT'C Cuts at: 2942 2942 Size: 7369 SalI G'TCGA_C Cuts at: 1936 4711 1936 Size: 2775 4594 SapI GCTCTTCn'nnn_ Cuts at: 2592 2592 Size: 7369 SbfI CC_TGCA'GG Cuts at: 4721 4721 Size: 7369 ScaI AGT'ACT Cuts at: 2271 4176 5617 7329 2271 Size: 1905 1441 1712 2311 Fragments arranged by size: 2311 1905 1712 1441 SfcI C'TryA_G Cuts at: 826 1045 2176 2256 2520 2655 3545 3852 4170 Size: 219 1131 80 264 135 890 307 318 Cuts at: 4170 4717 5852 6533 6724 826 Size: 547 1135 681 191 1471 Fragments arranged by size: 1471 1135 1131 890 681 547 318 307 264 219 191 135 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7369 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7369 SmaI CCC'GGG Cuts at: 1872 4696 1872 Size: 2824 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2918 5473 6374 6636 820 Size: 197 926 975 2555 901 262 1553 Fragments arranged by size: 2555 1553 975 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5160 493 Size: 4667 2702 SpeI A'CTAG_T Cuts at: 152 3803 152 Size: 3651 3718 SphI G_CATG'C Cuts at: 1132 4727 1132 Size: 3595 3774 SspI AAT'ATT Cuts at: 5 52 2197 5293 5 Size: 47 2145 3096 2081 Fragments arranged by size: 3096 2145 2081 47 StuI AGG'CCT Cuts at: 1517 3677 1517 Size: 2160 5209 StyI C'CwwG_G Cuts at: 513 1065 2004 2592 3116 3318 3686 4113 4371 Size: 552 939 588 524 202 368 427 258 Cuts at: 4371 4590 4808 513 Size: 219 218 3074 Fragments arranged by size: 3074 939 588 552 524 427 368 258 219 218 202 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4126 5396 5413 5566 5751 6196 325 Size: 1255 2546 1270 17 153 185 445 1498 Fragments arranged by size: 2546 1498 1270 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2269 3770 4174 Size: 274 80 33 51 1443 292 1501 404 Cuts at: 4174 5615 7327 96 Size: 1441 1712 138 Fragments arranged by size: 1712 1501 1443 1441 404 292 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2458 2490 7329 7361 2458 Size: 32 4839 32 2466 Fragments arranged by size: 4839 2466 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7369 XbaI T'CTAG_A Cuts at: 4705 4705 Size: 7369 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7369 XmaI C'CCGG_G Cuts at: 1870 4694 1870 Size: 2824 4545 XmnI GAAnn'nnTTC Cuts at: 3012 3963 4542 5498 3012 Size: 951 579 956 4883 Fragments arranged by size: 4883 956 951 579 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5177 276 Size: 53 83 186 936 3643 2468 Fragments arranged by size: 3643 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I FseI KasI NaeI NarI NgoMIV NruI NsiI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI