(Circular) (Six-base) MAPSORT of: pFN21AA0772.seq Check: 3190 from: 1 to: 6109 pFN21AA0772 6109 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2684 2684 Size: 6109 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3452 1937 Size: 1515 4594 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2173 2228 4172 5556 5824 1632 Size: 541 55 1944 1384 268 1917 Fragments arranged by size: 1944 1917 1384 541 268 55 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 2307 5008 5985 1203 Size: 1104 2701 977 1327 Fragments arranged by size: 2701 1327 1104 977 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3068 1184 Size: 1884 4225 AlwNI CAG_nnn'CTG Cuts at: 2347 3852 5424 2347 Size: 1505 1572 3032 Fragments arranged by size: 3032 1572 1505 ApaLI G'TGCA_C Cuts at: 4166 5322 4166 Size: 1156 4953 ApoI r'AATT_y Cuts at: 1493 2021 2136 3382 3418 3644 3796 5905 1493 Size: 528 115 1246 36 226 152 2109 1697 Fragments arranged by size: 2109 1697 1246 528 226 152 115 36 AseI AT'TA_AT Cuts at: 160 1032 2453 4663 160 Size: 872 1421 2210 1606 Fragments arranged by size: 2210 1606 1421 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 2613 3434 1870 Size: 73 670 821 4545 Fragments arranged by size: 4545 821 670 73 BamHI G'GATC_C Cuts at: 3439 3806 3439 Size: 367 5742 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2618 3430 4885 618 Size: 325 217 627 831 812 1455 1842 Fragments arranged by size: 1842 1455 831 812 627 325 217 BanII G_rGCy'C Cuts at: 1391 2613 3428 1391 Size: 1222 815 4072 Fragments arranged by size: 4072 1222 815 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2976 3158 928 Size: 971 1077 182 3879 Fragments arranged by size: 3879 1077 971 182 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4300 4334 1903 Size: 34 2363 34 3678 Fragments arranged by size: 3678 2363 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4720 136 Size: 107 122 71 631 605 3048 1525 Fragments arranged by size: 3048 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6104 6104 Size: 6109 BlpI GC'TnA_GC Cuts at: 3526 3526 Size: 6109 Bme1580I G_kGCm'C Cuts at: 1790 4170 5326 1790 Size: 2380 1156 2573 Fragments arranged by size: 2573 2380 1156 BmgBI CAC'GTC Cuts at: 1318 2995 3007 1318 Size: 1677 12 4420 Fragments arranged by size: 4420 1677 12 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2118 2392 3348 Size: 416 53 83 399 24 701 274 956 Cuts at: 3348 3840 4798 442 Size: 492 958 1753 Fragments arranged by size: 1753 958 956 701 492 416 399 274 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2137 2139 2137 Size: 2 6107 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 2369 2651 3079 3155 3266 4769 1239 Size: 1007 123 282 428 76 111 1503 2579 Fragments arranged by size: 2579 1503 1007 428 282 123 111 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2109 2564 4234 5099 5397 2109 Size: 455 1670 865 298 2821 Fragments arranged by size: 2821 1670 865 455 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2540 3017 4772 882 Size: 617 1041 477 1755 2219 Fragments arranged by size: 2219 1755 1041 617 477 BsaAI yAC'GTr Cuts at: 493 1204 2308 3900 493 Size: 711 1104 1592 2702 Fragments arranged by size: 2702 1592 1104 711 BsaBI GATnn'nnATC Cuts at: 2249 2482 6103 2249 Size: 233 3621 2255 Fragments arranged by size: 3621 2255 233 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3916 4298 4933 275 Size: 53 83 186 936 2383 382 635 1451 Fragments arranged by size: 2383 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4542 4940 5214 5361 5772 4542 Size: 398 274 147 411 4879 Fragments arranged by size: 4879 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2294 2312 2324 2342 3281 Size: 30 165 30 891 18 12 18 939 Cuts at: 3281 3311 1178 Size: 30 3976 Fragments arranged by size: 3976 939 891 165 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2947 3263 3302 1186 Size: 1761 316 39 3993 Fragments arranged by size: 3993 1761 316 39 BseYI C'CCAG_C Cuts at: 2340 5312 5808 2340 Size: 2972 496 2641 Fragments arranged by size: 2972 2641 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3050 1163 Size: 573 1314 4222 Fragments arranged by size: 4222 1314 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3559 4320 5348 1540 Size: 275 186 1558 761 1028 2301 Fragments arranged by size: 2301 1558 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 4170 4255 5326 2307 Size: 1863 85 1071 3090 Fragments arranged by size: 3090 1863 1071 85 BsmI GAATG_Cn' Cuts at: 3620 3713 3620 Size: 93 6016 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2307 2613 3428 4170 4255 5326 1391 Size: 399 517 306 815 742 85 1071 2174 Fragments arranged by size: 2174 1071 815 742 517 399 306 85 BspEI T'CCGG_A Cuts at: 5772 5772 Size: 6109 BspHI T'CATG_A Cuts at: 3998 5728 3998 Size: 1730 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2684 3022 3464 844 Size: 1840 338 442 3489 Fragments arranged by size: 3489 1840 442 338 BsrBI CCG'CTC Cuts at: 1394 1823 3241 3277 3379 3996 1394 Size: 429 1418 36 102 617 3507 Fragments arranged by size: 3507 1418 617 429 102 36 BsrDI GCAATG_nn' Cuts at: 65 1248 4604 4778 65 Size: 1183 3356 174 1396 Fragments arranged by size: 3356 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2161 2411 4753 4940 1700 Size: 110 351 250 2342 187 2869 Fragments arranged by size: 2869 2342 351 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 4169 5181 4169 Size: 1012 5097 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6109 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 2939 1184 Size: 1755 4354 BstXI CCAn_nnnn'nTGG Cuts at: 2629 2753 2629 Size: 124 5985 BstYI r'GATC_y Cuts at: 1972 2896 3217 3439 3806 4191 4208 5660 5746 Size: 924 321 222 367 385 17 1452 86 Cuts at: 5746 5758 6104 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 924 385 367 346 321 222 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2557 3165 3193 513 Size: 552 1492 608 28 3429 Fragments arranged by size: 3429 1492 608 552 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2139 487 Size: 683 56 913 4457 Fragments arranged by size: 4457 913 683 56 BtsI GCAGTG_nn' Cuts at: 3625 4419 4439 3625 Size: 794 20 5295 Fragments arranged by size: 5295 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6074 DraI TTT'AAA Cuts at: 2742 2779 3413 2742 Size: 37 634 5438 Fragments arranged by size: 5438 634 37 DraIII CAC_nnn'GTG Cuts at: 2815 2815 Size: 6109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3222 3556 4445 Size: 54 1425 50 275 18 1392 334 889 Cuts at: 4445 6074 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1392 889 334 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3556 1537 Size: 275 1744 4090 Fragments arranged by size: 4090 1744 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4039 1353 Size: 2686 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2621 4696 5070 5216 387 Size: 1064 138 1032 2075 374 146 1280 Fragments arranged by size: 2075 1280 1064 1032 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2228 2246 2369 2651 3079 3155 Size: 393 541 55 18 123 282 428 76 Cuts at: 3155 3266 4172 4769 5556 5824 1239 Size: 111 906 597 787 268 1524 Fragments arranged by size: 1524 906 787 597 541 428 393 282 268 123 111 76 55 18 EcoNI CCTnn'n_nnAGG Cuts at: 2951 2951 Size: 6109 EcoO109I rG'GnC_Cy Cuts at: 2118 2325 3342 2118 Size: 207 1017 4885 Fragments arranged by size: 4885 1017 207 EcoRI G'AATT_C Cuts at: 2021 2136 3418 3796 2021 Size: 115 1282 378 4334 Fragments arranged by size: 4334 1282 378 115 EcoRV GAT'ATC Cuts at: 3403 3403 Size: 6109 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2869 2869 Size: 6109 FspI TGC'GCA Cuts at: 4615 4615 Size: 6109 HaeII r_GCGC'y Cuts at: 1678 5256 1678 Size: 3578 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2607 2639 2957 2989 4750 Size: 32 135 32 1035 32 318 32 1761 Cuts at: 4750 4782 4824 4856 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1761 1035 318 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3453 3699 669 Size: 988 281 1515 246 3079 Fragments arranged by size: 3079 1515 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6109 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 3708 3708 Size: 6109 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3414 5223 5407 1305 Size: 2109 1809 184 2007 Fragments arranged by size: 2109 2007 1809 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3224 10 Size: 54 1425 343 1392 2895 Fragments arranged by size: 2895 1425 1392 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 2763 4067 4426 4585 518 Size: 648 897 700 1304 359 159 2042 Fragments arranged by size: 2042 1304 897 700 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2284 3195 3525 4202 5350 5595 1606 Size: 678 911 330 677 1148 245 2120 Fragments arranged by size: 2120 1148 911 678 677 330 245 NaeI GCC'GGC Cuts at: 2163 2163 Size: 6109 NcoI C'CATG_G Cuts at: 513 1065 2557 513 Size: 552 1492 4065 Fragments arranged by size: 4065 1492 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NgoMIV G'CCGG_C Cuts at: 2161 2161 Size: 6109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 3467 5012 1132 Size: 97 2238 1545 2229 Fragments arranged by size: 2238 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6109 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 2347 1108 Size: 1239 4870 PfoI T'CCnGG_A Cuts at: 2975 2975 Size: 6109 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PmlI CAC'GTG Cuts at: 2308 2308 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3342 3342 Size: 6109 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6109 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PstI C_TGCA'G Cuts at: 830 3461 830 Size: 2631 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 SacII CC_GC'GG Cuts at: 3196 3196 Size: 6109 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SanDI GG'GwC_CC Cuts at: 3342 3342 Size: 6109 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SfcI C'TryA_G Cuts at: 826 1045 3457 4592 5273 5464 826 Size: 219 2412 1135 681 191 1471 Fragments arranged by size: 2412 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 3436 1872 Size: 1564 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2124 2579 4213 5114 5376 820 Size: 197 926 181 455 1634 901 262 1553 Fragments arranged by size: 1634 1553 926 901 455 262 197 181 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3467 1132 Size: 2335 3774 SspI AAT'ATT Cuts at: 5 52 4033 5 Size: 47 3981 2081 Fragments arranged by size: 3981 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6109 StyI C'CwwG_G Cuts at: 513 1065 2557 3282 3548 513 Size: 552 1492 725 266 3074 Fragments arranged by size: 3074 1492 725 552 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3178 4136 4153 4306 4491 4936 325 Size: 1255 1598 958 17 153 185 445 1498 Fragments arranged by size: 1598 1498 1255 958 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 4355 6067 Size: 274 80 33 51 1443 318 2060 1712 Cuts at: 6067 96 Size: 138 Fragments arranged by size: 2060 1712 1443 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2312 2344 2870 2902 3281 3313 6069 6101 2312 Size: 32 526 32 379 32 2756 32 2320 Fragments arranged by size: 2756 2320 526 379 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6109 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6109 XmaI C'CCGG_G Cuts at: 1870 3434 1870 Size: 1564 4545 XmnI GAAnn'nnTTC Cuts at: 4238 4238 Size: 6109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI