Restriction map for
pFN21AA0767
(Linear) (Six-base) MAPSORT of: KIAA0767.seq Check: 3428 from: 1 to: 1734
KIAA0767.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AatII G_ACGT'C
Cuts at: 0 914 1734
Size: 914 820
AccI GT'mk_AC
Cuts at: 0 692 1612 1734
Size: 692 920 122
Fragments arranged by size:
920 692 122
Acc65I G'GTAC_C
Cuts at: 0 117 1734
Size: 117 1617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 415 1734
Size: 133 282 1319
Fragments arranged by size:
1319 282 133
AfeI AGC'GCT
Cuts at: 0 1213 1734
Size: 1213 521
AflIII A'CryG_T
Cuts at: 0 695 1734
Size: 695 1039
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 529 561 1734
Size: 529 32 1173
Fragments arranged by size:
1173 529 32
AlwNI CAG_nnn'CTG
Cuts at: 0 339 483 1011 1720 1734
Size: 339 144 528 709 14
Fragments arranged by size:
709 528 339 144 14
ApaLI G'TGCA_C
Cuts at: 0 1654 1734
Size: 1654 80
AvaI C'yCGr_G
Cuts at: 0 134 553 1137 1734
Size: 134 419 584 597
Fragments arranged by size:
597 584 419 134
BanI G'GyrC_C
Cuts at: 0 117 631 1645 1734
Size: 117 514 1014 89
Fragments arranged by size:
1014 514 117 89
BanII G_rGCy'C
Cuts at: 0 293 854 1734
Size: 293 561 880
Fragments arranged by size:
880 561 293
BbsI GAAGACnn'nnnn_
Cuts at: 0 878 1734
Size: 878 856
BbvCI CC'TCA_GC
Cuts at: 0 151 1707 1734
Size: 151 1556 27
Fragments arranged by size:
1556 151 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 812 1734
Size: 812 922
BglI GCCn_nnn'nGGC
Cuts at: 0 1039 1734
Size: 1039 695
BglII A'GATC_T
Cuts at: 0 1346 1734
Size: 1346 388
BlpI GC'TnA_GC
Cuts at: 0 422 1734
Size: 422 1312
Bme1580I G_kGCm'C
Cuts at: 0 839 1658 1734
Size: 839 819 76
Fragments arranged by size:
839 819 76
BmgBI CAC'GTC
Cuts at: 0 698 1734
Size: 698 1036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 979 1197 1200 1734
Size: 391 588 218 3 534
Fragments arranged by size:
588 534 391 218 3
Bpu10I CC'TnA_GC
Cuts at: 0 4 151 586 1590 1707 1734
Size: 4 147 435 1004 117 27
Fragments arranged by size:
1004 435 147 117 27 4
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1189 1734
Size: 607 582 545
Fragments arranged by size:
607 582 545
BsaI GGTCTCn'nnnn_
Cuts at: 0 1669 1734
Size: 1669 65
BsaAI yAC'GTr
Cuts at: 0 531 1266 1734
Size: 531 735 468
Fragments arranged by size:
735 531 468
BsaHI Gr'CG_yC
Cuts at: 0 911 1304 1615 1734
Size: 911 393 311 119
Fragments arranged by size:
911 393 311 119
BsaWI w'CCGG_w
Cuts at: 0 209 293 542 1424 1734
Size: 209 84 249 882 310
Fragments arranged by size:
882 310 249 209 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 670 700 1734
Size: 670 30 1034
Fragments arranged by size:
1034 670 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 301 424 571 1194 1312 1734
Size: 160 141 123 147 623 118 422
Fragments arranged by size:
623 422 160 147 141 123 118
BseYI C'CCAG_C
Cuts at: 0 743 1270 1734
Size: 743 527 464
Fragments arranged by size:
743 527 464
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1734
Size: 403 1331
BsiEI CG_ry'CG
Cuts at: 0 1283 1311 1734
Size: 1283 28 423
Fragments arranged by size:
1283 423 28
BsiHKAI G_wGCw'C
Cuts at: 0 293 1463 1658 1734
Size: 293 1170 195 76
Fragments arranged by size:
1170 293 195 76
BsmI GAATG_Cn'
Cuts at: 0 97 732 1734
Size: 97 635 1002
Fragments arranged by size:
1002 635 97
BsmBI CGTCTCn'nnnn_
Cuts at: 0 609 1734
Size: 609 1125
Bsp1286I G_dGCh'C
Cuts at: 0 293 839 854 1463 1658 1734
Size: 293 546 15 609 195 76
Fragments arranged by size:
609 546 293 195 76 15
BspEI T'CCGG_A
Cuts at: 0 293 1734
Size: 293 1441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 587 1734
Size: 587 1147
BsrBI CCG'CTC
Cuts at: 0 351 516 1635 1734
Size: 351 165 1119 99
Fragments arranged by size:
1119 351 165 99
BsrDI GCAATG_nn'
Cuts at: 0 462 704 1734
Size: 462 242 1030
Fragments arranged by size:
1030 462 242
BsrFI r'CCGG_y
Cuts at: 0 639 1278 1282 1306 1734
Size: 639 639 4 24 428
Fragments arranged by size:
639 639 428 24 4
BsrGI T'GTAC_A
Cuts at: 0 719 1734
Size: 719 1015
BssHII G'CGCG_C
Cuts at: 0 1146 1734
Size: 1146 588
BssSI C'ACGA_G
Cuts at: 0 1342 1636 1734
Size: 1342 294 98
Fragments arranged by size:
1342 294 98
BstAPI GCAn_nnn'nTGC
Cuts at: 0 381 390 1734
Size: 381 9 1344
Fragments arranged by size:
1344 381 9
BstBI TT'CG_AA
Cuts at: 0 1490 1734
Size: 1490 244
BstEII G'GTnAC_C
Cuts at: 0 244 1734
Size: 244 1490
BstYI r'GATC_y
Cuts at: 0 1346 1734
Size: 1346 388
BtgI C'CryG_G
Cuts at: 0 121 854 1079 1436 1649 1734
Size: 121 733 225 357 213 85
Fragments arranged by size:
733 357 225 213 121 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 786 1734
Size: 786 948
DraI TTT'AAA
Cuts at: 0 1128 1734
Size: 1128 606
DrdI GACnn_nn'nnGTC
Cuts at: 0 1251 1734
Size: 1251 483
EaeI y'GGCC_r
Cuts at: 0 185 1280 1734
Size: 185 1095 454
Fragments arranged by size:
1095 454 185
EagI C'GGCC_G
Cuts at: 0 1280 1734
Size: 1280 454
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 375 831 1734
Size: 375 456 903
Fragments arranged by size:
903 456 375
EcoICRI GAG'CTC
Cuts at: 0 291 1734
Size: 291 1443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 391 415 979 1197 1200 1734
Size: 133 258 24 564 218 3 534
Fragments arranged by size:
564 534 258 218 133 24 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1515 1734
Size: 1515 219
EcoO109I rG'GnC_Cy
Cuts at: 0 156 173 1508 1569 1734
Size: 156 17 1335 61 165
Fragments arranged by size:
1335 165 156 61 17
FseI GG_CCGG'CC
Cuts at: 0 1286 1734
Size: 1286 448
HaeII r_GCGC'y
Cuts at: 0 471 593 1024 1215 1223 1404 1734
Size: 471 122 431 191 8 181 330
Fragments arranged by size:
471 431 330 191 181 122 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 228 260 670 702 1338 1370 1734
Size: 228 32 410 32 636 32 364
Fragments arranged by size:
636 410 364 228 32 32 32
HincII GTy'rAC
Cuts at: 0 602 1613 1734
Size: 602 1011 121
Fragments arranged by size:
1011 602 121
HpaI GTT'AAC
Cuts at: 0 602 1734
Size: 602 1132
KpnI G_GTAC'C
Cuts at: 0 121 1734
Size: 121 1613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1734
Size: 701 1033
MscI TGG'CCA
Cuts at: 0 187 1734
Size: 187 1547
MslI CAynn'nnrTG
Cuts at: 0 1661 1734
Size: 1661 73
MspA1I CmG'CkG
Cuts at: 0 123 357 900 1377 1711 1734
Size: 123 234 543 477 334 23
Fragments arranged by size:
543 477 334 234 123 23
NaeI GCC'GGC
Cuts at: 0 1284 1734
Size: 1284 450
NcoI C'CATG_G
Cuts at: 0 1649 1734
Size: 1649 85
NgoMIV G'CCGG_C
Cuts at: 0 1282 1734
Size: 1282 452
PflMI CCAn_nnn'nTGG
Cuts at: 0 812 1734
Size: 812 922
PfoI T'CCnGG_A
Cuts at: 0 197 956 1734
Size: 197 759 778
Fragments arranged by size:
778 759 197
PpuMI rG'GwC_Cy
Cuts at: 0 156 173 1508 1569 1734
Size: 156 17 1335 61 165
Fragments arranged by size:
1335 165 156 61 17
PshAI GACnn'nnGTC
Cuts at: 0 1307 1734
Size: 1307 427
PstI C_TGCA'G
Cuts at: 0 500 1244 1734
Size: 500 744 490
Fragments arranged by size:
744 500 490
PvuII CAG'CTG
Cuts at: 0 900 1734
Size: 900 834
SacI G_AGCT'C
Cuts at: 0 293 1734
Size: 293 1441
SacII CC_GC'GG
Cuts at: 0 124 1734
Size: 124 1610
SalI G'TCGA_C
Cuts at: 0 1611 1734
Size: 1611 123
SanDI GG'GwC_CC
Cuts at: 0 156 173 1734
Size: 156 17 1561
Fragments arranged by size:
1561 156 17
SfcI C'TryA_G
Cuts at: 0 496 1240 1734
Size: 496 744 494
Fragments arranged by size:
744 496 494
SmaI CCC'GGG
Cuts at: 0 136 1734
Size: 136 1598
SmlI C'TyrA_G
Cuts at: 0 622 1204 1734
Size: 622 582 530
Fragments arranged by size:
622 582 530
SnaBI TAC'GTA
Cuts at: 0 1266 1734
Size: 1266 468
StuI AGG'CCT
Cuts at: 0 1669 1734
Size: 1669 65
StyI C'CwwG_G
Cuts at: 0 1649 1734
Size: 1649 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1639 1734
Size: 1639 95
TatI w'GTAC_w
Cuts at: 0 719 1562 1691 1734
Size: 719 843 129 43
Fragments arranged by size:
843 719 129 43
Tth111I GACn'n_nGTC
Cuts at: 0 882 1251 1734
Size: 882 369 483
Fragments arranged by size:
882 483 369
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 454 1734
Size: 454 1280
XmaI C'CCGG_G
Cuts at: 0 134 1734
Size: 134 1600
XmnI GAAnn'nnTTC
Cuts at: 0 505 934 976 1734
Size: 505 429 42 758
Fragments arranged by size:
758 505 429 42
ZraI GAC'GTC
Cuts at: 0 912 1734
Size: 912 822
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AloI AlwNI
ApaLI AvaI BanI BanII BbsI BbvCI BciVI BglI
BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI DraI DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII HpaI
KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
PflMI PfoI PpuMI PshAI PstI PvuII SacI SacII
SalI SanDI SfcI SmaI SmlI SnaBI StuI StyI
TaqII TatI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AgeI AhdI AleI AlfI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BclI BfrBI BmrI BmtI BplI BsaBI BsiWI
BspHI BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraIII
EarI EcoRI EcoRV FalI FspI FspAI HindIII KasI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SpeI SphI SrfI
SspI SwaI TstI XbaI XhoI