Restriction map for
pFN21AA0734
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# NO CUT ENZYMES ( 66 enzymes) #
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AccIII AclI AcsI ApoI AscI
AseI AsnI Asp700I BbeI BbrPI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspLU11I BssHII BstBI BstXI
ClaI CpoI DraI DrdI EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KasI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI TfiI Tsp509I XbaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 454 GGTACC G^GTACC
AflII 3132 CTTAAG C^TTAAG
AflIII 2315 ACGTGT A^C[GA][CT]GT
AgeI 3026 ACCGGT A^CCGGT
AhdI 3319 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 704 GTGCAC G^TGCAC
AocI 2907 CCTGAGG CC^T[ACGT]AGG
ApaLI 704 GTGCAC G^TGCAC
Asp718I 454 GGTACC G^GTACC
AspEI 3319 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1804 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3363 CCTAGG C^CTAGG
BcgI 56 GCACCGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1157 TGATCA T^GATCA
BfaI 3364 CTAG C^TAG
BfrI 3132 CTTAAG C^TTAAG
BlnI 3363 CCTAGG C^CTAGG
BsaAI 579 CACGTA [CT]AC^GT[GA]
BsaWI 3026 ACCGGT [AT]^CCGG[AT]
BsmBI 2814 CGTCTC CGTCTC(1/5)
BsrDI 1689 CATTGC GCAATG(2/0)
BssSI 2434 CTCGTG CACGAG(-5/-1)
Bst1107I 3232 GTATAC GTA^TAC
BstZ17I 3232 GTATAC GTA^TAC
Bsu36I 2907 CCTGAGG CC^T[ACGT]AGG
DraIII 2569 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2381 CGGCCG C^GGCCG
Eam1105I 3319 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2381 CGGCCG C^GGCCG
Eco52I 2381 CGGCCG C^GGCCG
Eco81I 2907 CCTGAGG CC^T[ACGT]AGG
EcoNI 2186 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1157 TGATCA T^GATCA
KpnI 454 GGTACC GGTAC^C
KspI 3101 CCGCGG CCGC^GG
MaeI 3364 CTAG C^TAG
NaeI 3443 GCCGGC GCC^GGC
NcoI 458 CCATGG C^CATGG
NgoMIV 3443 GCCGGC G^CCGGC
PaeR7I 319 CTCGAG C^TCGAG
PflFI 1804 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3026 ACCGGT A^CCGGT
PshAI 1744 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3101 CCGCGG CCGC^GG
SphI 659 GCATGC GCATG^C
StuI 2456 AGGCCT AGG^CCT
Tth111I 1804 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 319 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AatII 1876 GACGTC GACGT^C
2812 GACGTC
Aor51HI 719 AGCGCT AGC^GCT
2018 AGCGCT
AviII 332 TGCGCA TGC^GCA
2438 TGCGCA
BamHI 366 GGATCC G^GATCC
1733 GGATCC
BbsI 3210 GAAGAC GAAGAC(2/6)
1879 GTCTTC
BciVI 1173 GGATAC GTATCC(6/5)
898 GTATCC
BglII 1655 AGATCT A^GATCT
3463 AGATCT
BpuAI 3210 GAAGAC GAAGAC(2/6)
1879 GTCTTC
BsaI 3 GAGACC GGTCTC(1/5)
2221 GGTCTC
BsiEI 1083 CGACCG CG[GA][CT]^CG
2381 CGGCCG
Bsp1407I 431 TGTACA T^GTACA
2622 TGTACA
BspHI 885 TCATGA T^CATGA
2681 TCATGA
BsrBI 2052 CCGCTC CCGCTC(-3/-3)
2677 CCGCTC
BsrGI 431 TGTACA T^GTACA
2622 TGTACA
Eco47III 719 AGCGCT AGC^GCT
2018 AGCGCT
FspI 332 TGCGCA TGC^GCA
2438 TGCGCA
HincII 108 GTCGAC GT[CT]^[GA]AC
1081 GTCGAC
HindII 108 GTCGAC GT[CT]^[GA]AC
1081 GTCGAC
MseI 125 TTAA T^TAA
3133 TTAA
PflMI 1274 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1593 CCACCGCCTGG
RcaI 885 TCATGA T^CATGA
2681 TCATGA
SalI 108 GTCGAC G^TCGAC
1081 GTCGAC
SapI 792 GAAGAGC GCTCTTC(1/4)
1635 GCTCTTC
SexAI 470 ACCAGGT A^CC[AT]GGT
1142 ACCTGGT
SmaI 8 CCCGGG CCC^GGG
241 CCCGGG
SspBI 431 TGTACA T^GTACA
2622 TGTACA
Tru9I 125 TTAA T^TAA
3133 TTAA
Van91I 1274 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1593 CCACCGCCTGG
XmaCI 8 CCCGGG C^CCGGG
241 CCCGGG
XmaI 8 CCCGGG C^CCGGG
241 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XhoII