(Circular) (Six-base) MAPSORT of: pFN21AA0734.seq Check: 157 from: 1 to: 8266 pFN21AA0734 8266 bp With 182 enzymes: SgfI * March 28, 2012 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2140 2146 2506 3231 2140 Size: 6 360 725 7175 Fragments arranged by size: 7175 725 360 6 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3885 4821 6076 278 Size: 53 83 186 936 2349 936 1255 2468 Fragments arranged by size: 2468 2349 1255 936 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2114 3087 5238 5609 1937 Size: 177 973 2151 371 4594 Fragments arranged by size: 4594 2151 973 371 177 Acc65I G'GTAC_C Cuts at: 2459 5587 2459 Size: 3128 5138 AclI AA'CG_TT Cuts at: 1563 6393 6766 1563 Size: 4830 373 3063 Fragments arranged by size: 4830 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2309 2632 2969 3638 3728 3812 4133 4958 Size: 677 323 337 669 90 84 321 825 Cuts at: 4958 6329 7713 7981 1632 Size: 1371 1384 268 1917 Fragments arranged by size: 1917 1384 1371 825 677 669 337 323 321 268 90 84 AfeI AGC'GCT Cuts at: 2726 4025 2726 Size: 1299 6967 AflII C'TTAA_G Cuts at: 820 1017 5137 820 Size: 197 4120 3949 Fragments arranged by size: 4120 3949 197 AflIII A'CryG_T Cuts at: 1203 4320 7165 8142 1203 Size: 3117 2845 977 1327 Fragments arranged by size: 3117 2845 1327 977 AgeI A'CCGG_T Cuts at: 5031 7097 5031 Size: 2066 6200 AhdI GACnn_n'nnGTC Cuts at: 5329 6995 5329 Size: 1666 6600 AleI CACnn'nnGTG Cuts at: 1166 2792 1166 Size: 1626 6640 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2241 1184 Size: 1057 7209 AlwNI CAG_nnn'CTG Cuts at: 3285 3550 3784 4220 4297 4546 4801 5083 6009 Size: 265 234 436 77 249 255 282 926 Cuts at: 6009 7581 3285 Size: 1572 3970 Fragments arranged by size: 3970 1572 926 436 282 265 255 249 234 77 ApaI G_GGCC'C Cuts at: 2039 2471 4503 5171 2039 Size: 432 2032 668 5134 Fragments arranged by size: 5134 2032 668 432 ApaLI G'TGCA_C Cuts at: 2709 6323 7479 2709 Size: 3614 1156 3496 Fragments arranged by size: 3614 3496 1156 ApoI r'AATT_y Cuts at: 1493 5575 5801 5953 8062 1493 Size: 4082 226 152 2109 1697 Fragments arranged by size: 4082 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6820 160 Size: 872 5788 1606 Fragments arranged by size: 5788 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8266 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2246 2324 3759 3982 4502 5591 Size: 73 70 233 78 1435 223 520 1089 Cuts at: 5591 1870 Size: 4545 Fragments arranged by size: 4545 1435 1089 520 233 223 78 73 70 AvrII C'CTAG_G Cuts at: 5368 5368 Size: 8266 BamHI G'GATC_C Cuts at: 2371 3738 5596 5963 2371 Size: 1367 1858 367 4674 Fragments arranged by size: 4674 1858 1367 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2459 2525 3045 3070 Size: 325 217 627 273 399 66 520 25 Cuts at: 3070 4301 5587 7042 618 Size: 1231 1286 1455 1842 Fragments arranged by size: 1842 1455 1286 1231 627 520 399 325 273 217 66 25 BanII G_rGCy'C Cuts at: 1391 2039 2395 2471 2706 3273 3300 3340 3959 Size: 648 356 76 235 567 27 40 619 Cuts at: 3959 3990 3999 4182 4440 4503 5171 5461 5585 Size: 31 9 183 258 63 668 290 124 Cuts at: 5585 1391 Size: 4072 Fragments arranged by size: 4072 668 648 619 567 356 290 258 235 183 124 76 63 40 31 27 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3877 5222 928 Size: 971 1978 1345 3972 Fragments arranged by size: 3972 1978 1345 971 BbvCI CC'TCA_GC Cuts at: 3924 4124 4235 5477 3924 Size: 200 111 1242 6713 Fragments arranged by size: 6713 1242 200 111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2050 2084 6457 6491 1903 Size: 34 113 34 4373 34 3678 Fragments arranged by size: 4373 3678 113 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2914 3172 6158 7368 2914 Size: 258 2986 1210 3812 Fragments arranged by size: 3812 2986 1210 258 BclI T'GATC_A Cuts at: 1548 3162 1548 Size: 1614 6652 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2234 2952 5373 Size: 107 122 71 631 605 562 718 2421 Cuts at: 5373 6877 136 Size: 1504 1525 Fragments arranged by size: 2421 1525 1504 718 631 605 562 122 107 71 BglII A'GATC_T Cuts at: 3660 5468 8261 3660 Size: 1808 2793 3665 Fragments arranged by size: 3665 2793 1808 BlpI GC'TnA_GC Cuts at: 2501 3233 3299 3953 4529 5073 5683 2501 Size: 732 66 654 576 544 610 5084 Fragments arranged by size: 5084 732 654 610 576 544 66 Bme1580I G_kGCm'C Cuts at: 1790 2039 2063 2165 2471 2713 4304 4503 5171 Size: 249 24 102 306 242 1591 199 668 Cuts at: 5171 6327 7483 1790 Size: 1156 1156 2573 Fragments arranged by size: 2573 1591 1156 1156 668 306 249 242 199 102 24 BmgBI CAC'GTC Cuts at: 1318 4321 1318 Size: 3003 5263 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3472 5997 6955 Size: 416 53 83 399 24 2055 2525 958 Cuts at: 6955 442 Size: 1753 Fragments arranged by size: 2525 2055 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8266 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3130 3292 3314 3997 3130 Size: 162 22 683 7399 Fragments arranged by size: 7399 683 162 22 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2110 2495 3100 3125 3262 3464 3626 3929 Size: 871 385 605 25 137 202 162 303 Cuts at: 3929 3992 4004 4180 4196 4766 4778 4877 4925 Size: 63 12 176 16 570 12 99 48 Cuts at: 4925 5042 5561 6926 1239 Size: 117 519 1365 2579 Fragments arranged by size: 2579 1365 871 605 570 519 385 303 202 176 162 137 117 99 63 48 25 16 12 12 Bpu10I CC'TnA_GC Cuts at: 2144 3924 4124 4235 4290 4745 5045 5477 2144 Size: 1780 200 111 55 455 300 432 4933 Fragments arranged by size: 4933 1780 455 432 300 200 111 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2555 3140 5516 6391 7256 7554 2555 Size: 585 2376 875 865 298 3267 Fragments arranged by size: 3267 2376 875 865 585 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2002 4232 6929 882 Size: 617 503 2230 2697 2219 Fragments arranged by size: 2697 2230 2219 617 503 BsaAI yAC'GTr Cuts at: 493 1204 2586 6057 493 Size: 711 1382 3471 2702 Fragments arranged by size: 3471 2702 1382 711 BsaBI GATnn'nnATC Cuts at: 8260 8260 Size: 8266 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2691 3084 3810 3882 Size: 53 83 186 936 1158 393 726 72 Cuts at: 3882 4188 4818 5041 5127 5376 6073 6455 7090 Size: 306 630 223 86 249 697 382 635 Cuts at: 7090 275 Size: 1451 Fragments arranged by size: 1451 1158 936 726 697 635 630 393 382 306 249 223 186 86 83 72 53 BsaWI w'CCGG_w Cuts at: 5031 6699 7097 7371 7518 7929 5031 Size: 1668 398 274 147 411 5368 Fragments arranged by size: 5368 1668 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2420 2450 4178 4193 4208 Size: 30 165 30 1017 30 1728 15 15 Cuts at: 4208 4223 4751 4781 1178 Size: 15 528 30 4663 Fragments arranged by size: 4663 1728 1017 528 165 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2107 2420 2441 2504 2552 3281 3306 3512 Size: 921 313 21 63 48 729 25 206 Cuts at: 3512 3941 3962 4183 4769 4958 5494 1186 Size: 429 21 221 586 189 536 3958 Fragments arranged by size: 3958 921 729 586 536 429 313 221 206 189 63 48 25 21 21 BseYI C'CCAG_C Cuts at: 2375 2668 3433 4297 4305 4454 4801 5164 5348 Size: 293 765 864 8 149 347 363 184 Cuts at: 5348 5420 7469 7965 2375 Size: 72 2049 496 2676 Fragments arranged by size: 2676 2049 864 765 496 363 347 293 184 149 72 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 2174 2855 2941 3152 3314 3359 Size: 573 300 138 681 86 211 162 45 Cuts at: 3359 3394 4519 4940 5084 5213 5423 1163 Size: 35 1125 421 144 129 210 4006 Fragments arranged by size: 4006 1125 681 573 421 300 211 210 162 144 138 129 86 45 35 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 4389 5716 6477 7505 1540 Size: 275 186 1090 1298 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1298 1090 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2544 2713 2832 2861 3258 3300 3999 4440 Size: 399 169 119 29 397 42 699 441 Cuts at: 4440 4515 6327 6412 7483 2145 Size: 75 1812 85 1071 2928 Fragments arranged by size: 2928 1812 1071 699 441 399 397 169 119 85 75 42 29 BsmI GAATG_Cn' Cuts at: 5777 5870 5777 Size: 93 8173 BsmBI CGTCTCn'nnnn_ Cuts at: 4825 4825 Size: 8266 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2063 2145 2165 2395 2471 2544 Size: 399 249 24 82 20 230 76 73 Cuts at: 2544 2706 2713 2832 2861 3258 3273 3300 3340 Size: 162 7 119 29 397 15 27 40 Cuts at: 3340 3959 3990 3999 4182 4304 4440 4503 4515 Size: 619 31 9 183 122 136 63 12 Cuts at: 4515 5171 5461 5585 6327 6412 7483 1391 Size: 656 290 124 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 656 619 399 397 290 249 230 183 162 136 124 122 119 85 82 76 73 63 40 31 29 27 24 20 15 12 9 7 BspEI T'CCGG_A Cuts at: 7929 7929 Size: 8266 BspHI T'CATG_A Cuts at: 2890 4686 6155 7885 2890 Size: 1796 1469 1730 3271 Fragments arranged by size: 3271 1796 1730 1469 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2058 2140 2146 2506 2952 3231 3373 3416 Size: 1214 82 6 360 446 279 142 43 Cuts at: 3416 3438 5121 5621 844 Size: 22 1683 500 3489 Fragments arranged by size: 3489 1683 1214 500 446 360 279 142 82 43 22 6 BsrBI CCG'CTC Cuts at: 1394 1823 4059 4684 6153 1394 Size: 429 2236 625 1469 3507 Fragments arranged by size: 3507 2236 1469 625 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3693 6761 6935 65 Size: 1183 2445 3068 174 1396 Fragments arranged by size: 3068 2445 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4388 5031 5448 6910 7097 1700 Size: 110 2578 643 417 1462 187 2869 Fragments arranged by size: 2869 2578 1462 643 417 187 110 BsrGI T'GTAC_A Cuts at: 96 2436 4627 96 Size: 2340 2191 3735 Fragments arranged by size: 3735 2340 2191 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8266 BssSI C'ACGA_G Cuts at: 4439 6326 7338 4439 Size: 1887 1012 5367 Fragments arranged by size: 5367 1887 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2027 3550 4220 794 Size: 1233 1523 670 4840 Fragments arranged by size: 4840 1523 1233 670 BstBI TT'CG_AA Cuts at: 7070 7070 Size: 8266 BstEII G'GTnAC_C Cuts at: 1184 2045 2100 2785 1184 Size: 861 55 685 6665 Fragments arranged by size: 6665 861 685 55 BstYI r'GATC_y Cuts at: 1972 2371 3660 3738 5468 5596 5963 6348 6365 Size: 399 1289 78 1730 128 367 385 17 Cuts at: 6365 7817 7903 7915 8261 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1730 1452 1289 399 385 367 346 128 86 78 17 12 BstZ17I GTA'TAC Cuts at: 5239 5239 Size: 8266 Bsu36I CC'TnA_GG Cuts at: 4913 4913 Size: 8266 BtgI C'CryG_G Cuts at: 513 1065 2229 2463 4158 4182 4494 4674 5088 Size: 552 1164 234 1695 24 312 180 414 Cuts at: 5088 5106 513 Size: 18 3673 Fragments arranged by size: 3673 1695 1164 552 414 312 234 180 24 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7527 Fragments arranged by size: 7527 683 56 BtsI GCAGTG_nn' Cuts at: 2347 3139 3479 4218 4404 5782 6576 6596 2347 Size: 792 340 739 186 1378 794 20 4017 Fragments arranged by size: 4017 1378 794 792 739 340 186 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8266 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2737 2772 529 Size: 35 2173 35 6023 Fragments arranged by size: 6023 2173 35 35 DraI TTT'AAA Cuts at: 5570 5570 Size: 8266 DraIII CAC_nnn'GTG Cuts at: 4579 4579 Size: 8266 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7273 809 Size: 507 5957 1802 Fragments arranged by size: 5957 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 3183 3366 Size: 54 1425 50 275 18 798 555 183 Cuts at: 3366 4045 4312 4386 4481 5085 5713 6602 8231 Size: 679 267 74 95 604 628 889 1629 Cuts at: 8231 8 Size: 43 Fragments arranged by size: 1629 1425 889 798 679 628 604 555 275 267 183 95 74 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4386 5713 1537 Size: 275 2574 1327 4090 Fragments arranged by size: 4090 2574 1327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2792 2843 3462 3647 3704 3863 4343 5255 Size: 1439 51 619 185 57 159 480 912 Cuts at: 5255 6196 1353 Size: 941 3423 Fragments arranged by size: 3423 1439 941 912 619 480 185 159 57 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2162 3511 5573 6853 7227 7373 Size: 1064 138 573 1349 2062 1280 374 146 Cuts at: 7373 387 Size: 1280 Fragments arranged by size: 2062 1349 1280 1280 1064 573 374 146 138 EcoICRI GAG'CTC Cuts at: 3298 3997 4438 3298 Size: 699 441 7126 Fragments arranged by size: 7126 699 441 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2309 2495 2632 2969 3100 3125 Size: 393 478 199 186 137 337 131 25 Cuts at: 3125 3262 3464 3626 3638 3728 3812 3929 3992 Size: 137 202 162 12 90 84 117 63 Cuts at: 3992 4004 4133 4180 4196 4766 4778 4877 4925 Size: 12 129 47 16 570 12 99 48 Cuts at: 4925 4958 5042 5561 6329 6926 7713 7981 1239 Size: 33 84 519 768 597 787 268 1524 Fragments arranged by size: 1524 787 768 597 570 519 478 393 337 268 202 199 186 162 137 137 131 129 117 99 90 84 84 63 48 47 33 25 16 12 12 12 EcoNI CCTnn'n_nnAGG Cuts at: 4195 4195 Size: 8266 EcoO109I rG'GnC_Cy Cuts at: 2191 2235 2329 2431 2468 2494 2578 2635 3011 Size: 44 94 102 37 26 84 57 376 Cuts at: 3011 4471 4499 4543 4762 4861 5016 5205 2191 Size: 1460 28 44 219 99 155 189 5252 Fragments arranged by size: 5252 1460 376 219 189 155 102 99 94 84 57 44 44 37 28 26 EcoRI G'AATT_C Cuts at: 5575 5953 5575 Size: 378 7888 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2848 2848 Size: 8266 FspI TGC'GCA Cuts at: 2339 4445 6772 2339 Size: 2106 2327 3833 Fragments arranged by size: 3833 2327 2106 HaeII r_GCGC'y Cuts at: 1678 2211 2728 3784 4027 4412 4802 7413 1678 Size: 533 517 1056 243 385 390 2611 2531 Fragments arranged by size: 2611 2531 1056 533 517 390 385 243 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2888 2920 4043 4075 4178 Size: 32 135 32 1316 32 1123 32 103 Cuts at: 4178 4210 5315 5347 6907 6939 6981 7013 1373 Size: 32 1105 32 1560 32 42 32 2626 Fragments arranged by size: 2626 1560 1316 1123 1105 135 103 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2115 3088 5610 5856 669 Size: 988 281 177 973 2522 246 3079 Fragments arranged by size: 3079 2522 988 973 281 246 177 HindIII A'AGCT_T Cuts at: 748 748 Size: 8266 HpaI GTT'AAC Cuts at: 5856 5856 Size: 8266 KpnI G_GTAC'C Cuts at: 2463 5591 2463 Size: 3128 5138 MfeI C'AATT_G Cuts at: 5865 5865 Size: 8266 MluI A'CGCG_T Cuts at: 8142 8142 Size: 8266 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3231 3657 3670 7380 7564 1305 Size: 1926 426 13 3710 184 2007 Fragments arranged by size: 3710 2007 1926 426 184 13 MscI TGG'CCA Cuts at: 10 64 1489 1832 2630 4314 4483 10 Size: 54 1425 343 798 1684 169 3793 Fragments arranged by size: 3793 1684 1425 798 343 169 54 MslI CAynn'nnrTG Cuts at: 518 1166 2792 3030 5312 6224 6583 6742 518 Size: 648 1626 238 2282 912 359 159 2042 Fragments arranged by size: 2282 2042 1626 912 648 359 238 159 MspA1I CmG'CkG Cuts at: 1606 2033 2869 3093 3285 3364 3547 3593 3907 Size: 427 836 224 192 79 183 46 314 Cuts at: 3907 4533 5080 5108 5682 6359 7507 7752 1606 Size: 626 547 28 574 677 1148 245 2120 Fragments arranged by size: 2120 1148 836 677 626 574 547 427 314 245 224 192 183 79 46 28 NaeI GCC'GGC Cuts at: 5450 5450 Size: 8266 NcoI C'CATG_G Cuts at: 513 1065 2463 513 Size: 552 1398 6316 Fragments arranged by size: 6316 1398 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8266 NgoMIV G'CCGG_C Cuts at: 5448 5448 Size: 8266 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8266 NotI GC'GGCC_GC Cuts at: 5713 5713 Size: 8266 NspI r_CATG'y Cuts at: 1132 1229 2278 2668 3454 5624 7169 1132 Size: 97 1049 390 786 2170 1545 2229 Fragments arranged by size: 2229 2170 1545 1049 786 390 97 PasI CC'CwG_GG Cuts at: 1348 2195 1348 Size: 847 7419 PciI A'CATG_T Cuts at: 7165 7165 Size: 8266 PflMI CCAn_nnn'nTGG Cuts at: 1108 3285 3604 1108 Size: 2177 319 5770 Fragments arranged by size: 5770 2177 319 PfoI T'CCnGG_A Cuts at: 3063 4245 3063 Size: 1182 7084 PmeI GTTT'AAAC Cuts at: 5570 5570 Size: 8266 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6335 6367 7848 7880 6335 Size: 32 1481 32 6721 Fragments arranged by size: 6721 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2191 2329 2635 3011 5016 5205 2191 Size: 138 306 376 2005 189 5252 Fragments arranged by size: 5252 2005 376 306 189 138 PshAI GACnn'nnGTC Cuts at: 1613 3753 1613 Size: 2140 6126 PsiI TTA'TAA Cuts at: 5836 5836 Size: 8266 PspOMI G'GGCC_C Cuts at: 2035 2467 4499 5167 2035 Size: 432 2032 668 5134 Fragments arranged by size: 5134 2032 668 432 PspXI vC'TCGA_Gb Cuts at: 2324 2324 Size: 8266 PstI C_TGCA'G Cuts at: 830 3546 3639 4797 5618 830 Size: 2716 93 1158 821 3478 Fragments arranged by size: 3478 2716 1158 821 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8266 PvuII CAG'CTG Cuts at: 2869 3285 3364 3547 3907 4533 2869 Size: 416 79 183 360 626 6602 Fragments arranged by size: 6602 626 416 360 183 79 SacI G_AGCT'C Cuts at: 3300 3999 4440 3300 Size: 699 441 7126 Fragments arranged by size: 7126 699 441 SacII CC_GC'GG Cuts at: 5109 5109 Size: 8266 SalI G'TCGA_C Cuts at: 1936 2113 3086 5608 1936 Size: 177 973 2522 4594 Fragments arranged by size: 4594 2522 973 177 SapI GCTCTTCn'nnn_ Cuts at: 2792 3647 2792 Size: 855 7411 SbfI CC_TGCA'GG Cuts at: 5618 5618 Size: 8266 ScaI AGT'ACT Cuts at: 6514 8226 6514 Size: 1712 6554 SexAI A'CCwGG_T Cuts at: 2475 3147 2475 Size: 672 7594 SfcI C'TryA_G Cuts at: 826 1045 3421 3542 3635 3838 4793 4834 5614 Size: 219 2376 121 93 203 955 41 780 Cuts at: 5614 6749 7430 7621 826 Size: 1135 681 191 1471 Fragments arranged by size: 2376 1471 1135 955 780 681 219 203 191 121 93 41 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8266 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8266 SmaI CCC'GGG Cuts at: 1872 2015 2248 5593 1872 Size: 143 233 3345 4545 Fragments arranged by size: 4545 3345 233 143 SmlI C'TyrA_G Cuts at: 820 1017 1943 2324 2534 3155 5137 5531 6370 Size: 197 926 381 210 621 1982 394 839 Cuts at: 6370 7271 7533 820 Size: 901 262 1553 Fragments arranged by size: 1982 1553 926 901 839 621 394 381 262 210 197 SnaBI TAC'GTA Cuts at: 493 6057 493 Size: 5564 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8266 SphI G_CATG'C Cuts at: 1132 2668 5624 1132 Size: 1536 2956 3774 Fragments arranged by size: 3774 2956 1536 SspI AAT'ATT Cuts at: 5 52 6190 5 Size: 47 6138 2081 Fragments arranged by size: 6138 2081 47 StuI AGG'CCT Cuts at: 1517 4463 1517 Size: 2946 5320 StyI C'CwwG_G Cuts at: 513 1065 2463 2574 2631 5368 5705 513 Size: 552 1398 111 57 2737 337 3074 Fragments arranged by size: 3074 2737 1398 552 337 111 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2088 3925 4246 4319 4793 6293 6310 Size: 1255 508 1837 321 73 474 1500 17 Cuts at: 6310 6463 6648 7093 325 Size: 153 185 445 1498 Fragments arranged by size: 1837 1500 1498 1255 508 474 445 321 185 153 73 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2436 4627 6512 Size: 274 80 33 51 1443 459 2191 1885 Cuts at: 6512 8224 96 Size: 1712 138 Fragments arranged by size: 2191 1885 1712 1443 459 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4193 4225 4751 4783 4949 4981 8226 8258 4193 Size: 32 526 32 166 32 3245 32 4201 Fragments arranged by size: 4201 3245 526 166 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3812 1316 Size: 2496 5770 XbaI T'CTAG_A Cuts at: 5602 5602 Size: 8266 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2519 2623 3967 2519 Size: 104 1344 6818 Fragments arranged by size: 6818 1344 104 XhoI C'TCGA_G Cuts at: 1943 2324 1943 Size: 381 7885 XmaI C'CCGG_G Cuts at: 1870 2013 2246 5591 1870 Size: 143 233 3345 4545 Fragments arranged by size: 4545 3345 233 143 XmnI GAAnn'nnTTC Cuts at: 6395 6395 Size: 8266 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3883 4819 6074 276 Size: 53 83 186 936 2349 936 1255 2468 Fragments arranged by size: 2468 2349 1255 936 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BfrBI BsiWI BstXI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SanDI SfiI SfoI SrfI SwaI