Restriction map for
pFN21AA0734
(Linear) (Six-base) MAPSORT of: KIAA0734.seq Check: 9685 from: 1 to: 3561
KIAA0734.orf
With 182 enzymes: SgfI *
September 25, 2008 15:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 135 141 501 1226 3561
Size: 135 6 360 725 2335
Fragments arranged by size:
2335 725 360 135 6
AatII G_ACGT'C
Cuts at: 0 1880 2816 3561
Size: 1880 936 745
Fragments arranged by size:
1880 936 745
AccI GT'mk_AC
Cuts at: 0 109 1082 3233 3561
Size: 109 973 2151 328
Fragments arranged by size:
2151 973 328 109
Acc65I G'GTAC_C
Cuts at: 0 454 3561
Size: 454 3107
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 627 964 1633 1723 1807 2128 2953
Size: 304 323 337 669 90 84 321 825
Cuts at: 2953 3561
Size: 608
Fragments arranged by size:
825 669 608 337 323 321 304 90
84
AfeI AGC'GCT
Cuts at: 0 721 2020 3561
Size: 721 1299 1541
Fragments arranged by size:
1541 1299 721
AflII C'TTAA_G
Cuts at: 0 3132 3561
Size: 3132 429
AflIII A'CryG_T
Cuts at: 0 2315 3561
Size: 2315 1246
AgeI A'CCGG_T
Cuts at: 0 3026 3561
Size: 3026 535
AhdI GACnn_n'nnGTC
Cuts at: 0 3324 3561
Size: 3324 237
AleI CACnn'nnGTG
Cuts at: 0 787 3561
Size: 787 2774
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 236 3561
Size: 236 3325
AlwNI CAG_nnn'CTG
Cuts at: 0 1280 1545 1779 2215 2292 2541 2796 3078
Size: 1280 265 234 436 77 249 255 282
Cuts at: 3078 3561
Size: 483
Fragments arranged by size:
1280 483 436 282 265 255 249 234
77
ApaI G_GGCC'C
Cuts at: 0 34 466 2498 3166 3561
Size: 34 432 2032 668 395
Fragments arranged by size:
2032 668 432 395 34
ApaLI G'TGCA_C
Cuts at: 0 704 3561
Size: 704 2857
AvaI C'yCGr_G
Cuts at: 0 8 241 319 1754 1977 2497 3561
Size: 8 233 78 1435 223 520 1064
Fragments arranged by size:
1435 1064 520 233 223 78 8
AvrII C'CTAG_G
Cuts at: 0 3363 3561
Size: 3363 198
BamHI G'GATC_C
Cuts at: 0 366 1733 3561
Size: 366 1367 1828
Fragments arranged by size:
1828 1367 366
BanI G'GyrC_C
Cuts at: 0 55 454 520 1040 1065 2296 3561
Size: 55 399 66 520 25 1231 1265
Fragments arranged by size:
1265 1231 520 399 66 55 25
BanII G_rGCy'C
Cuts at: 0 34 390 466 701 1268 1295 1335 1954
Size: 34 356 76 235 567 27 40 619
Cuts at: 1954 1985 1994 2177 2435 2498 3166 3456 3561
Size: 31 9 183 258 63 668 290 105
Fragments arranged by size:
668 619 567 356 290 258 235 183
105 76 63 40 34 31 27 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1872 3217 3561
Size: 1872 1345 344
Fragments arranged by size:
1872 1345 344
BbvCI CC'TCA_GC
Cuts at: 0 1919 2119 2230 3472 3561
Size: 1919 200 111 1242 89
Fragments arranged by size:
1919 1242 200 111 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 45 79 3561
Size: 45 34 3482
Fragments arranged by size:
3482 45 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 909 1167 3561
Size: 909 258 2394
Fragments arranged by size:
2394 909 258
BclI T'GATC_A
Cuts at: 0 1157 3561
Size: 1157 2404
BglI GCCn_nnn'nGGC
Cuts at: 0 229 947 3368 3561
Size: 229 718 2421 193
Fragments arranged by size:
2421 718 229 193
BglII A'GATC_T
Cuts at: 0 1655 3463 3561
Size: 1655 1808 98
Fragments arranged by size:
1808 1655 98
BlpI GC'TnA_GC
Cuts at: 0 496 1228 1294 1948 2524 3068 3561
Size: 496 732 66 654 576 544 493
Fragments arranged by size:
732 654 576 544 496 493 66
Bme1580I G_kGCm'C
Cuts at: 0 34 58 160 466 708 2299 2498 3166
Size: 34 24 102 306 242 1591 199 668
Cuts at: 3166 3561
Size: 395
Fragments arranged by size:
1591 668 395 306 242 199 102 34
24
BmgBI CAC'GTC
Cuts at: 0 2316 3561
Size: 2316 1245
BmrI ACTGGGnnnn_n'
Cuts at: 0 1467 3561
Size: 1467 2094
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1125 1287 1309 1992 3561
Size: 1125 162 22 683 1569
Fragments arranged by size:
1569 1125 683 162 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 490 1095 1120 1257 1459 1621 1924
Size: 105 385 605 25 137 202 162 303
Cuts at: 1924 1987 1999 2175 2191 2761 2773 2872 2920
Size: 63 12 176 16 570 12 99 48
Cuts at: 2920 3037 3556 3561
Size: 117 519 5
Fragments arranged by size:
605 570 519 385 303 202 176 162
137 117 105 99 63 48 25 16
12 12 5
Bpu10I CC'TnA_GC
Cuts at: 0 139 1919 2119 2230 2285 2740 3040 3472
Size: 139 1780 200 111 55 455 300 432
Cuts at: 3472 3561
Size: 89
Fragments arranged by size:
1780 455 432 300 200 139 111 89
55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1135 3511 3561
Size: 550 585 2376 50
Fragments arranged by size:
2376 585 550 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 2227 3561
Size: 2227 1334
BsaAI yAC'GTr
Cuts at: 0 581 3561
Size: 581 2980
BsaHI Gr'CG_yC
Cuts at: 0 686 1079 1805 1877 2183 2813 3036 3122
Size: 686 393 726 72 306 630 223 86
Cuts at: 3122 3371 3561
Size: 249 190
Fragments arranged by size:
726 686 630 393 306 249 223 190
86 72
BsaWI w'CCGG_w
Cuts at: 0 3026 3561
Size: 3026 535
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 445 2173 2188 2203 2218 2746 2776
Size: 415 30 1728 15 15 15 528 30
Cuts at: 2776 3561
Size: 785
Fragments arranged by size:
1728 785 528 415 30 30 15 15
15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 102 415 436 499 547 1276 1301 1507
Size: 102 313 21 63 48 729 25 206
Cuts at: 1507 1936 1957 2178 2764 2953 3489 3561
Size: 429 21 221 586 189 536 72
Fragments arranged by size:
729 586 536 429 313 221 206 189
102 72 63 48 25 21 21
BseYI C'CCAG_C
Cuts at: 0 370 663 1428 2292 2300 2449 2796 3159
Size: 370 293 765 864 8 149 347 363
Cuts at: 3159 3343 3415 3561
Size: 184 72 146
Fragments arranged by size:
864 765 370 363 347 293 184 149
146 72 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 169 850 936 1147 1309 1354 1389
Size: 31 138 681 86 211 162 45 35
Cuts at: 1389 2514 2935 3079 3208 3418 3561
Size: 1125 421 144 129 210 143
Fragments arranged by size:
1125 681 421 211 210 162 144 143
138 129 86 45 35 31
BsiEI CG_ry'CG
Cuts at: 0 1086 2384 3561
Size: 1086 1298 1177
Fragments arranged by size:
1298 1177 1086
BsiHKAI G_wGCw'C
Cuts at: 0 140 539 708 827 856 1253 1295 1994
Size: 140 399 169 119 29 397 42 699
Cuts at: 1994 2435 2510 3561
Size: 441 75 1051
Fragments arranged by size:
1051 699 441 399 397 169 140 119
75 42 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2820 3561
Size: 2820 741
Bsp1286I G_dGCh'C
Cuts at: 0 34 58 140 160 390 466 539 701
Size: 34 24 82 20 230 76 73 162
Cuts at: 701 708 827 856 1253 1268 1295 1335 1954
Size: 7 119 29 397 15 27 40 619
Cuts at: 1954 1985 1994 2177 2299 2435 2498 2510 3166
Size: 31 9 183 122 136 63 12 656
Cuts at: 3166 3456 3561
Size: 290 105
Fragments arranged by size:
656 619 397 290 230 183 162 136
122 119 105 82 76 73 63 40
34 31 29 27 24 20 15 12
9 7
BspHI T'CATG_A
Cuts at: 0 885 2681 3561
Size: 885 1796 880
Fragments arranged by size:
1796 885 880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 135 141 501 947 1226 1368 1411
Size: 53 82 6 360 446 279 142 43
Cuts at: 1411 1433 3116 3561
Size: 22 1683 445
Fragments arranged by size:
1683 446 445 360 279 142 82 53
43 22 6
BsrBI CCG'CTC
Cuts at: 0 2054 2679 3561
Size: 2054 625 882
Fragments arranged by size:
2054 882 625
BsrDI GCAATG_nn'
Cuts at: 0 1688 3561
Size: 1688 1873
BsrFI r'CCGG_y
Cuts at: 0 2383 3026 3443 3561
Size: 2383 643 417 118
Fragments arranged by size:
2383 643 417 118
BsrGI T'GTAC_A
Cuts at: 0 431 2622 3561
Size: 431 2191 939
Fragments arranged by size:
2191 939 431
BssSI C'ACGA_G
Cuts at: 0 2434 3561
Size: 2434 1127
BstAPI GCAn_nnn'nTGC
Cuts at: 0 22 1545 2215 3561
Size: 22 1523 670 1346
Fragments arranged by size:
1523 1346 670 22
BstEII G'GTnAC_C
Cuts at: 0 40 95 780 3561
Size: 40 55 685 2781
Fragments arranged by size:
2781 685 55 40
BstYI r'GATC_y
Cuts at: 0 366 1655 1733 3463 3561
Size: 366 1289 78 1730 98
Fragments arranged by size:
1730 1289 366 98 78
BstZ17I GTA'TAC
Cuts at: 0 3234 3561
Size: 3234 327
Bsu36I CC'TnA_GG
Cuts at: 0 2908 3561
Size: 2908 653
BtgI C'CryG_G
Cuts at: 0 224 458 2153 2177 2489 2669 3083 3101
Size: 224 234 1695 24 312 180 414 18
Cuts at: 3101 3561
Size: 460
Fragments arranged by size:
1695 460 414 312 234 224 180 24
18
BtsI GCAGTG_nn'
Cuts at: 0 342 1134 1474 2213 2399 3561
Size: 342 792 340 739 186 1162
Fragments arranged by size:
1162 792 739 342 340 186
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 732 767 3561
Size: 732 35 2794
Fragments arranged by size:
2794 732 35
DraIII CAC_nnn'GTG
Cuts at: 0 2574 3561
Size: 2574 987
EaeI y'GGCC_r
Cuts at: 0 623 1178 1361 2040 2307 2381 2476 3080
Size: 623 555 183 679 267 74 95 604
Cuts at: 3080 3561
Size: 481
Fragments arranged by size:
679 623 604 555 481 267 183 95
74
EagI C'GGCC_G
Cuts at: 0 2381 3561
Size: 2381 1180
EarI CTCTTCn'nnn_
Cuts at: 0 787 838 1457 1642 1699 1858 2338 3250
Size: 787 51 619 185 57 159 480 912
Cuts at: 3250 3561
Size: 311
Fragments arranged by size:
912 787 619 480 311 185 159 57
51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 157 1506 3561
Size: 157 1349 2055
Fragments arranged by size:
2055 1349 157
EcoICRI GAG'CTC
Cuts at: 0 1293 1992 2433 3561
Size: 1293 699 441 1128
Fragments arranged by size:
1293 1128 699 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 304 490 627 964 1095 1120 1257
Size: 105 199 186 137 337 131 25 137
Cuts at: 1257 1459 1621 1633 1723 1807 1924 1987 1999
Size: 202 162 12 90 84 117 63 12
Cuts at: 1999 2128 2175 2191 2761 2773 2872 2920 2953
Size: 129 47 16 570 12 99 48 33
Cuts at: 2953 3037 3556 3561
Size: 84 519 5
Fragments arranged by size:
570 519 337 202 199 186 162 137
137 131 129 117 105 99 90 84
84 63 48 47 33 25 16 12
12 12 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2190 3561
Size: 2190 1371
EcoO109I rG'GnC_Cy
Cuts at: 0 186 230 324 426 463 489 573 630
Size: 186 44 94 102 37 26 84 57
Cuts at: 630 1006 2466 2494 2538 2757 2856 3011 3200
Size: 376 1460 28 44 219 99 155 189
Cuts at: 3200 3561
Size: 361
Fragments arranged by size:
1460 376 361 219 189 186 155 102
99 94 84 57 44 44 37 28
26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 843 3561
Size: 843 2718
FspI TGC'GCA
Cuts at: 0 334 2440 3561
Size: 334 2106 1121
Fragments arranged by size:
2106 1121 334
HaeII r_GCGC'y
Cuts at: 0 206 723 1779 2022 2407 2797 3561
Size: 206 517 1056 243 385 390 764
Fragments arranged by size:
1056 764 517 390 385 243 206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 883 915 2038 2070 2173 2205 3310 3342
Size: 883 32 1123 32 103 32 1105 32
Cuts at: 3342 3561
Size: 219
Fragments arranged by size:
1123 1105 883 219 103 32 32 32
32
HincII GTy'rAC
Cuts at: 0 110 1083 3561
Size: 110 973 2478
Fragments arranged by size:
2478 973 110
KpnI G_GTAC'C
Cuts at: 0 458 3561
Size: 458 3103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1226 1652 1665 3561
Size: 1226 426 13 1896
Fragments arranged by size:
1896 1226 426 13
MscI TGG'CCA
Cuts at: 0 625 2309 2478 3561
Size: 625 1684 169 1083
Fragments arranged by size:
1684 1083 625 169
MslI CAynn'nnrTG
Cuts at: 0 787 1025 3307 3561
Size: 787 238 2282 254
Fragments arranged by size:
2282 787 254 238
MspA1I CmG'CkG
Cuts at: 0 28 864 1088 1280 1359 1542 1588 1902
Size: 28 836 224 192 79 183 46 314
Cuts at: 1902 2528 3075 3103 3561
Size: 626 547 28 458
Fragments arranged by size:
836 626 547 458 314 224 192 183
79 46 28 28
NaeI GCC'GGC
Cuts at: 0 3445 3561
Size: 3445 116
NcoI C'CATG_G
Cuts at: 0 458 3561
Size: 458 3103
NgoMIV G'CCGG_C
Cuts at: 0 3443 3561
Size: 3443 118
NspI r_CATG'y
Cuts at: 0 273 663 1449 3561
Size: 273 390 786 2112
Fragments arranged by size:
2112 786 390 273
PasI CC'CwG_GG
Cuts at: 0 190 3561
Size: 190 3371
PflMI CCAn_nnn'nTGG
Cuts at: 0 1280 1599 3561
Size: 1280 319 1962
Fragments arranged by size:
1962 1280 319
PfoI T'CCnGG_A
Cuts at: 0 1058 2240 3561
Size: 1058 1182 1321
Fragments arranged by size:
1321 1182 1058
PpuMI rG'GwC_Cy
Cuts at: 0 186 324 630 1006 3011 3200 3561
Size: 186 138 306 376 2005 189 361
Fragments arranged by size:
2005 376 361 306 189 186 138
PshAI GACnn'nnGTC
Cuts at: 0 1748 3561
Size: 1748 1813
PspOMI G'GGCC_C
Cuts at: 0 30 462 2494 3162 3561
Size: 30 432 2032 668 399
Fragments arranged by size:
2032 668 432 399 30
PspXI vC'TCGA_Gb
Cuts at: 0 319 3561
Size: 319 3242
PstI C_TGCA'G
Cuts at: 0 1541 1634 2792 3561
Size: 1541 93 1158 769
Fragments arranged by size:
1541 1158 769 93
PvuII CAG'CTG
Cuts at: 0 864 1280 1359 1542 1902 2528 3561
Size: 864 416 79 183 360 626 1033
Fragments arranged by size:
1033 864 626 416 360 183 79
SacI G_AGCT'C
Cuts at: 0 1295 1994 2435 3561
Size: 1295 699 441 1126
Fragments arranged by size:
1295 1126 699 441
SacII CC_GC'GG
Cuts at: 0 3104 3561
Size: 3104 457
SalI G'TCGA_C
Cuts at: 0 108 1081 3561
Size: 108 973 2480
Fragments arranged by size:
2480 973 108
SapI GCTCTTCn'nnn_
Cuts at: 0 787 1642 3561
Size: 787 855 1919
Fragments arranged by size:
1919 855 787
SexAI A'CCwGG_T
Cuts at: 0 470 1142 3561
Size: 470 672 2419
Fragments arranged by size:
2419 672 470
SfcI C'TryA_G
Cuts at: 0 1416 1537 1630 1833 2788 2829 3561
Size: 1416 121 93 203 955 41 732
Fragments arranged by size:
1416 955 732 203 121 93 41
SmaI CCC'GGG
Cuts at: 0 10 243 3561
Size: 10 233 3318
Fragments arranged by size:
3318 233 10
SmlI C'TyrA_G
Cuts at: 0 319 529 1150 3132 3526 3561
Size: 319 210 621 1982 394 35
Fragments arranged by size:
1982 621 394 319 210 35
SphI G_CATG'C
Cuts at: 0 663 3561
Size: 663 2898
StuI AGG'CCT
Cuts at: 0 2458 3561
Size: 2458 1103
StyI C'CwwG_G
Cuts at: 0 458 569 626 3363 3561
Size: 458 111 57 2737 198
Fragments arranged by size:
2737 458 198 111 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 83 1920 2241 2314 2788 3561
Size: 83 1837 321 73 474 773
Fragments arranged by size:
1837 773 474 321 83 73
TatI w'GTAC_w
Cuts at: 0 431 2622 3561
Size: 431 2191 939
Fragments arranged by size:
2191 939 431
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2188 2220 2746 2778 2944 2976 3561
Size: 2188 32 526 32 166 32 585
Fragments arranged by size:
2188 585 526 166 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1807 3561
Size: 1807 1754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 514 618 1962 3561
Size: 514 104 1344 1599
Fragments arranged by size:
1599 1344 514 104
XhoI C'TCGA_G
Cuts at: 0 319 3561
Size: 319 3242
XmaI C'CCGG_G
Cuts at: 0 8 241 3561
Size: 8 233 3320
Fragments arranged by size:
3320 233 8
ZraI GAC'GTC
Cuts at: 0 1878 2814 3561
Size: 1878 936 747
Fragments arranged by size:
1878 936 747
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AvaI
AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII
SalI SapI SexAI SfcI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AclI AloI ApoI AscI AseI AsiSI BaeI BbeI
BfrBI BmtI BsaBI BsiWI BsmI BspEI BssHII BstBI
BstXI BtgZI ClaI DraI DrdI EcoRI EcoRV FseI
FspAI HindIII HpaI KasI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PsiI PsrI PvuI RsrII SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI XmnI