(Circular) (Six-base) MAPSORT of: pFN21AA0676.seq Check: 4413 from: 1 to: 8455 pFN21AA0676 8455 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4096 4096 Size: 8455 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6265 278 Size: 53 83 186 936 4729 2468 Fragments arranged by size: 4729 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2214 4068 5580 5798 1937 Size: 277 1854 1512 218 4594 Fragments arranged by size: 4594 1854 1512 277 218 Acc65I G'GTAC_C Cuts at: 5776 5776 Size: 8455 AclI AA'CG_TT Cuts at: 1563 6582 6955 1563 Size: 5019 373 3063 Fragments arranged by size: 5019 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2450 2855 3392 3542 5285 6518 7902 8170 Size: 818 405 537 150 1743 1233 1384 268 Cuts at: 8170 1632 Size: 1917 Fragments arranged by size: 1917 1743 1384 1233 818 537 405 268 150 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8258 AflIII A'CryG_T Cuts at: 1203 2612 5112 5457 7354 8331 1203 Size: 1409 2500 345 1897 977 1327 Fragments arranged by size: 2500 1897 1409 1327 977 345 AgeI A'CCGG_T Cuts at: 7286 7286 Size: 8455 AhdI GACnn_n'nnGTC Cuts at: 5458 7184 5458 Size: 1726 6729 AleI CACnn'nnGTG Cuts at: 1166 3896 4245 1166 Size: 2730 349 5376 Fragments arranged by size: 5376 2730 349 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4263 1184 Size: 3079 5376 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5696 5728 5696 Size: 32 8423 AlwNI CAG_nnn'CTG Cuts at: 2296 2496 2761 3367 3982 4147 4375 5143 5238 Size: 200 265 606 615 165 228 768 95 Cuts at: 5238 5431 5614 6198 7770 2296 Size: 193 183 584 1572 2981 Fragments arranged by size: 2981 1572 768 615 606 584 265 228 200 193 183 165 95 ApaLI G'TGCA_C Cuts at: 6512 7668 6512 Size: 1156 7299 ApoI r'AATT_y Cuts at: 1493 4322 5764 5990 6142 8251 1493 Size: 2829 1442 226 152 2109 1697 Fragments arranged by size: 2829 2109 1697 1442 226 152 AseI AT'TA_AT Cuts at: 160 1032 7009 160 Size: 872 5977 1606 Fragments arranged by size: 5977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8455 AvaI C'yCGr_G Cuts at: 1870 1943 2405 2645 2853 3599 3689 5780 1870 Size: 73 462 240 208 746 90 2091 4545 Fragments arranged by size: 4545 2091 746 462 240 208 90 73 AvrII C'CTAG_G Cuts at: 2798 2798 Size: 8455 BamHI G'GATC_C Cuts at: 5785 6152 5785 Size: 367 8088 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2135 3346 5573 5776 7231 Size: 325 217 627 348 1211 2227 203 1455 Cuts at: 7231 618 Size: 1842 Fragments arranged by size: 2227 1842 1455 1211 627 348 325 217 203 BanII G_rGCy'C Cuts at: 1391 2018 2682 3910 3939 4302 4805 5417 5495 Size: 627 664 1228 29 363 503 612 78 Cuts at: 5495 5774 1391 Size: 279 4072 Fragments arranged by size: 4072 1228 664 627 612 503 363 279 78 29 BbeI G_GCGC'C Cuts at: 3350 3350 Size: 8455 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2117 3252 4350 5130 5514 928 Size: 971 218 1135 1098 780 384 3869 Fragments arranged by size: 3869 1135 1098 971 780 384 218 BbvCI CC'TCA_GC Cuts at: 3314 4031 3314 Size: 717 7738 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3680 3714 4991 5025 6646 6680 1903 Size: 34 1743 34 1277 34 1621 34 3678 Fragments arranged by size: 3678 1743 1621 1277 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 6347 7557 3539 Size: 2808 1210 4437 Fragments arranged by size: 4437 2808 1210 BclI T'GATC_A Cuts at: 1548 4698 1548 Size: 3150 5305 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2171 2744 2809 Size: 107 122 71 631 605 499 573 65 Cuts at: 2809 3419 4829 5165 7066 136 Size: 610 1410 336 1901 1525 Fragments arranged by size: 1901 1525 1410 631 610 605 573 499 336 122 107 71 65 BglII A'GATC_T Cuts at: 4885 8450 4885 Size: 3565 4890 BlpI GC'TnA_GC Cuts at: 2012 2424 3612 4256 5872 2012 Size: 412 1188 644 1616 4595 Fragments arranged by size: 4595 1616 1188 644 412 Bme1580I G_kGCm'C Cuts at: 1790 2138 2167 2514 3514 6516 7672 1790 Size: 348 29 347 1000 3002 1156 2573 Fragments arranged by size: 3002 2573 1156 1000 348 347 29 BmgBI CAC'GTC Cuts at: 1318 2146 1318 Size: 828 7627 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2272 4522 6186 Size: 416 53 83 399 24 855 2250 1664 Cuts at: 6186 7144 442 Size: 958 1753 Fragments arranged by size: 2250 1753 1664 958 855 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4506 1056 Size: 3450 5005 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4242 4873 4242 Size: 631 7824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2143 2273 2639 2801 3061 3170 3979 4484 Size: 904 130 366 162 260 109 809 505 Cuts at: 4484 4586 4847 5763 7115 1239 Size: 102 261 916 1352 2579 Fragments arranged by size: 2579 1352 916 904 809 505 366 261 260 162 130 109 102 Bpu10I CC'TnA_GC Cuts at: 3314 4031 3314 Size: 717 7738 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4748 4899 5663 6580 7445 7743 4748 Size: 151 764 917 865 298 5460 Fragments arranged by size: 5460 917 865 764 298 151 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2705 2835 3663 5313 7118 882 Size: 617 1206 130 828 1650 1805 2219 Fragments arranged by size: 2219 1805 1650 1206 828 617 130 BsaAI yAC'GTr Cuts at: 493 1204 6246 493 Size: 711 5042 2702 Fragments arranged by size: 5042 2702 711 BsaBI GATnn'nnATC Cuts at: 2975 4890 8449 2975 Size: 1915 3559 2981 Fragments arranged by size: 3559 2981 1915 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2089 2850 2939 3347 Size: 53 83 186 936 556 761 89 408 Cuts at: 3347 4128 6262 6644 7279 275 Size: 781 2134 382 635 1451 Fragments arranged by size: 2134 1451 936 781 761 635 556 408 382 186 89 83 53 BsaWI w'CCGG_w Cuts at: 4575 4990 6888 7286 7560 7707 8118 4575 Size: 415 1898 398 274 147 411 4912 Fragments arranged by size: 4912 1898 415 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3897 3927 4972 5002 5696 Size: 30 165 30 2494 30 1045 30 694 Cuts at: 5696 5726 1178 Size: 30 3907 Fragments arranged by size: 3907 2494 1045 694 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 3026 3261 3455 3551 3671 3845 3848 Size: 850 990 235 194 96 120 174 3 Cuts at: 3848 4218 4426 4849 4863 4973 4980 4985 5264 Size: 370 208 423 14 110 7 5 279 Cuts at: 5264 1186 Size: 4377 Fragments arranged by size: 4377 990 850 423 370 279 235 208 194 174 120 110 96 14 7 5 3 BseYI C'CCAG_C Cuts at: 2569 3089 3265 3301 3715 4147 4183 4550 4792 Size: 520 176 36 414 432 36 367 242 Cuts at: 4792 5308 5424 7658 8154 2569 Size: 516 116 2234 496 2870 Fragments arranged by size: 2870 2234 520 516 496 432 414 367 242 176 116 36 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2615 2911 2939 3185 3659 4540 Size: 573 438 441 296 28 246 474 881 Cuts at: 4540 1163 Size: 5078 Fragments arranged by size: 5078 881 573 474 441 438 296 246 28 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4540 5905 6666 7694 1540 Size: 275 186 2539 1365 761 1028 2301 Fragments arranged by size: 2539 2301 1365 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2682 3696 3813 3939 4302 4773 6516 6601 7672 Size: 1014 117 126 363 471 1743 85 1071 Cuts at: 7672 2682 Size: 3465 Fragments arranged by size: 3465 1743 1071 1014 471 363 126 117 85 BsmI GAATG_Cn' Cuts at: 5966 6059 5966 Size: 93 8362 BsmBI CGTCTCn'nnnn_ Cuts at: 2388 5039 2388 Size: 2651 5804 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2138 2167 2514 2682 3514 3696 Size: 399 228 120 29 347 168 832 182 Cuts at: 3696 3813 3910 3939 4302 4773 4805 5417 5495 Size: 117 97 29 363 471 32 612 78 Cuts at: 5495 5774 6516 6601 7672 1391 Size: 279 742 85 1071 2174 Fragments arranged by size: 2174 1071 832 742 612 471 399 363 347 279 228 182 168 120 117 97 85 78 32 29 29 BspEI T'CCGG_A Cuts at: 4990 8118 4990 Size: 3128 5327 BspHI T'CATG_A Cuts at: 6344 8074 6344 Size: 1730 6725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2388 3681 4096 5810 844 Size: 1544 1293 415 1714 3489 Fragments arranged by size: 3489 1714 1544 1293 415 BsrBI CCG'CTC Cuts at: 1394 1823 2059 2905 3681 4731 6342 1394 Size: 429 236 846 776 1050 1611 3507 Fragments arranged by size: 3507 1611 1050 846 776 429 236 BsrDI GCAATG_nn' Cuts at: 65 1248 3795 5507 6950 7124 65 Size: 1183 2547 1712 1443 174 1396 Fragments arranged by size: 2547 1712 1443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4535 7099 7286 1700 Size: 110 2725 2564 187 2869 Fragments arranged by size: 2869 2725 2564 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8455 BssHII G'CGCG_C Cuts at: 1925 5561 1925 Size: 3636 4819 BssSI C'ACGA_G Cuts at: 2129 2588 3940 6515 7527 2129 Size: 459 1352 2575 1012 3057 Fragments arranged by size: 3057 2575 1352 1012 459 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8455 BstBI TT'CG_AA Cuts at: 3930 5011 7259 3930 Size: 1081 2248 5126 Fragments arranged by size: 5126 2248 1081 BstEII G'GTnAC_C Cuts at: 1184 3049 1184 Size: 1865 6590 BstXI CCAn_nnnn'nTGG Cuts at: 3241 3901 4498 3241 Size: 660 597 7198 Fragments arranged by size: 7198 660 597 BstYI r'GATC_y Cuts at: 1972 3171 4885 4891 5056 5610 5785 6152 6537 Size: 1199 1714 6 165 554 175 367 385 Cuts at: 6537 6554 8006 8092 8104 8450 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1714 1452 1199 554 385 367 346 175 165 86 17 12 6 Bsu36I CC'TnA_GG Cuts at: 2457 3041 3273 4334 2457 Size: 584 232 1061 6578 Fragments arranged by size: 6578 1061 584 232 BtgI C'CryG_G Cuts at: 513 1065 2098 3396 4056 4741 5130 5163 513 Size: 552 1033 1298 660 685 389 33 3805 Fragments arranged by size: 3805 1298 1033 685 660 552 389 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2344 4281 5140 487 Size: 683 56 1118 1937 859 3802 Fragments arranged by size: 3802 1937 1118 859 683 56 BtsI GCAGTG_nn' Cuts at: 2603 3832 5971 6765 6785 2603 Size: 1229 2139 794 20 4273 Fragments arranged by size: 4273 2139 1229 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8420 DraI TTT'AAA Cuts at: 5759 5759 Size: 8455 DraIII CAC_nnn'GTG Cuts at: 2934 2934 Size: 8455 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2210 7462 809 Size: 507 894 5252 1802 Fragments arranged by size: 5252 1802 894 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2736 2745 Size: 54 1425 50 275 18 204 702 9 Cuts at: 2745 2810 2914 2965 3648 4116 4404 4533 4537 Size: 65 104 51 683 468 288 129 4 Cuts at: 4537 5166 5902 6791 8420 8 Size: 629 736 889 1629 43 Fragments arranged by size: 1629 1425 889 736 702 683 629 468 288 275 204 129 104 65 54 51 50 43 18 9 4 EagI C'GGCC_G Cuts at: 1537 1812 4537 5902 1537 Size: 275 2725 1365 4090 Fragments arranged by size: 4090 2725 1365 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2361 2645 2687 3383 3569 3927 4031 4452 Size: 1008 284 42 696 186 358 104 421 Cuts at: 4452 4929 5654 6385 1353 Size: 477 725 731 3423 Fragments arranged by size: 3423 1008 731 725 696 477 421 358 284 186 104 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5355 7042 7416 7562 387 Size: 1064 138 3766 1687 374 146 1280 Fragments arranged by size: 3766 1687 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2680 3937 4300 2680 Size: 1257 363 6835 Fragments arranged by size: 6835 1257 363 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2143 2273 2450 2639 2801 2855 3061 Size: 393 511 130 177 189 162 54 206 Cuts at: 3061 3170 3392 3542 3979 4484 4586 4847 5285 Size: 109 222 150 437 505 102 261 438 Cuts at: 5285 5763 6518 7115 7902 8170 1239 Size: 478 755 597 787 268 1524 Fragments arranged by size: 1524 787 755 597 511 505 478 438 437 393 268 261 222 206 189 177 162 150 130 109 102 54 EcoNI CCTnn'n_nnAGG Cuts at: 3044 3044 Size: 8455 EcoO109I rG'GnC_Cy Cuts at: 2401 2791 3364 3403 3573 3979 4468 4546 4747 Size: 390 573 39 170 406 489 78 201 Cuts at: 4747 5140 2401 Size: 393 5716 Fragments arranged by size: 5716 573 489 406 393 390 201 170 78 39 EcoRI G'AATT_C Cuts at: 5764 6142 5764 Size: 378 8077 EcoRV GAT'ATC Cuts at: 2323 2323 Size: 8455 FseI GG_CCGG'CC Cuts at: 4539 4539 Size: 8455 FspI TGC'GCA Cuts at: 3339 6961 3339 Size: 3622 4833 HaeII r_GCGC'y Cuts at: 1678 3350 4056 7602 1678 Size: 1672 706 3546 2531 Fragments arranged by size: 3546 2531 1672 706 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3577 3609 4844 4876 4972 Size: 32 135 32 2005 32 1235 32 96 Cuts at: 4972 5004 5041 5073 5444 5476 5667 5699 7096 Size: 32 37 32 371 32 191 32 1397 Cuts at: 7096 7128 7170 7202 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2005 1397 1235 371 191 135 96 42 37 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2542 4069 4210 5581 5799 6045 Size: 988 281 604 1527 141 1371 218 246 Cuts at: 6045 669 Size: 3079 Fragments arranged by size: 3079 1527 1371 988 604 281 246 218 141 HindIII A'AGCT_T Cuts at: 748 748 Size: 8455 HpaI GTT'AAC Cuts at: 6045 6045 Size: 8455 KasI G'GCGC_C Cuts at: 3346 3346 Size: 8455 KpnI G_GTAC'C Cuts at: 5780 5780 Size: 8455 MfeI C'AATT_G Cuts at: 6054 6054 Size: 8455 MluI A'CGCG_T Cuts at: 2612 8331 2612 Size: 5719 2736 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3006 3264 7569 7753 1305 Size: 1701 258 4305 184 2007 Fragments arranged by size: 4305 2007 1701 258 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 2738 2747 2967 3650 Size: 54 1425 343 204 702 9 220 683 Cuts at: 3650 5168 10 Size: 1518 3297 Fragments arranged by size: 3297 1518 1425 702 683 343 220 204 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2657 3896 4094 4178 4245 5462 6413 Size: 648 1491 1239 198 84 67 1217 951 Cuts at: 6413 6772 6931 518 Size: 359 159 2042 Fragments arranged by size: 2042 1491 1239 1217 951 648 359 198 159 84 67 MspA1I CmG'CkG Cuts at: 1606 2758 4144 5312 5400 5428 5871 6548 7696 Size: 1152 1386 1168 88 28 443 677 1148 Cuts at: 7696 7941 1606 Size: 245 2120 Fragments arranged by size: 2120 1386 1168 1152 1148 677 443 245 88 28 NaeI GCC'GGC Cuts at: 4537 4537 Size: 8455 NarI GG'CG_CC Cuts at: 3347 3347 Size: 8455 NcoI C'CATG_G Cuts at: 513 1065 3396 5130 513 Size: 552 2331 1734 3838 Fragments arranged by size: 3838 2331 1734 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8455 NgoMIV G'CCGG_C Cuts at: 4535 4535 Size: 8455 NheI G'CTAG_C Cuts at: 1052 4502 1052 Size: 3450 5005 NotI GC'GGCC_GC Cuts at: 5902 5902 Size: 8455 NspI r_CATG'y Cuts at: 1132 1229 3490 3874 4666 5116 5461 5813 7358 Size: 97 2261 384 792 450 345 352 1545 Cuts at: 7358 1132 Size: 2229 Fragments arranged by size: 2261 2229 1545 792 450 384 352 345 97 PasI CC'CwG_GG Cuts at: 1348 2389 2895 3361 4623 4905 5232 1348 Size: 1041 506 466 1262 282 327 4571 Fragments arranged by size: 4571 1262 1041 506 466 327 282 PciI A'CATG_T Cuts at: 5112 5457 7354 5112 Size: 345 1897 6213 Fragments arranged by size: 6213 1897 345 PflMI CCAn_nnn'nTGG Cuts at: 1108 4393 5383 1108 Size: 3285 990 4180 Fragments arranged by size: 4180 3285 990 PfoI T'CCnGG_A Cuts at: 2778 4122 4596 4674 2778 Size: 1344 474 78 6559 Fragments arranged by size: 6559 1344 474 78 PmeI GTTT'AAAC Cuts at: 5759 5759 Size: 8455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5696 5728 6524 6556 8037 8069 5696 Size: 32 796 32 1481 32 6082 Fragments arranged by size: 6082 1481 796 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2401 3403 3979 4468 4546 4747 5140 2401 Size: 1002 576 489 78 201 393 5716 Fragments arranged by size: 5716 1002 576 489 393 201 78 PshAI GACnn'nnGTC Cuts at: 1613 3056 1613 Size: 1443 7012 PsiI TTA'TAA Cuts at: 6025 6025 Size: 8455 PstI C_TGCA'G Cuts at: 830 2085 2385 2492 4107 4157 4282 5317 5552 Size: 1255 300 107 1615 50 125 1035 235 Cuts at: 5552 5807 830 Size: 255 3478 Fragments arranged by size: 3478 1615 1255 1035 300 255 235 125 107 50 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8455 PvuII CAG'CTG Cuts at: 2758 4144 5312 5428 2758 Size: 1386 1168 116 5785 Fragments arranged by size: 5785 1386 1168 116 SacI G_AGCT'C Cuts at: 2682 3939 4302 2682 Size: 1257 363 6835 Fragments arranged by size: 6835 1257 363 SalI G'TCGA_C Cuts at: 1936 4067 5579 5797 1936 Size: 2131 1512 218 4594 Fragments arranged by size: 4594 2131 1512 218 SanDI GG'GwC_CC Cuts at: 4546 4546 Size: 8455 SapI GCTCTTCn'nnn_ Cuts at: 2645 2687 3383 3927 4452 4929 2645 Size: 42 696 544 525 477 6171 Fragments arranged by size: 6171 696 544 525 477 42 SbfI CC_TGCA'GG Cuts at: 4107 5807 4107 Size: 1700 6755 ScaI AGT'ACT Cuts at: 4511 6703 8415 4511 Size: 2192 1712 4551 Fragments arranged by size: 4551 2192 1712 SfcI C'TryA_G Cuts at: 826 1045 2081 2381 2488 4103 4153 4278 5313 Size: 219 1036 300 107 1615 50 125 1035 Cuts at: 5313 5548 5803 6938 7619 7810 826 Size: 235 255 1135 681 191 1471 Fragments arranged by size: 1615 1471 1135 1036 1035 681 300 255 235 219 191 125 107 50 SfiI GGCCn_nnn'nGGCC Cuts at: 2744 2809 2744 Size: 65 8390 SfoI GGC'GCC Cuts at: 3348 3348 Size: 8455 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8455 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8455 SmaI CCC'GGG Cuts at: 1872 2407 3601 5782 1872 Size: 535 1194 2181 4545 Fragments arranged by size: 4545 2181 1194 535 SmlI C'TyrA_G Cuts at: 820 1017 1943 4763 4914 5678 6559 7460 7722 Size: 197 926 2820 151 764 881 901 262 Cuts at: 7722 820 Size: 1553 Fragments arranged by size: 2820 1553 926 901 881 764 262 197 151 SnaBI TAC'GTA Cuts at: 493 6246 493 Size: 5753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8455 SphI G_CATG'C Cuts at: 1132 3874 5813 1132 Size: 2742 1939 3774 Fragments arranged by size: 3774 2742 1939 SspI AAT'ATT Cuts at: 5 52 6379 5 Size: 47 6327 2081 Fragments arranged by size: 6327 2081 47 StuI AGG'CCT Cuts at: 1517 3839 5546 1517 Size: 2322 1707 4426 Fragments arranged by size: 4426 2322 1707 StyI C'CwwG_G Cuts at: 513 1065 2798 3396 3406 3414 3423 3919 4392 Size: 552 1733 598 10 8 9 496 473 Cuts at: 4392 5022 5130 5894 513 Size: 630 108 764 3074 Fragments arranged by size: 3074 1733 764 630 598 552 496 473 108 10 9 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 2590 6482 6499 6652 6837 7282 Size: 1255 458 552 3892 17 153 185 445 Cuts at: 7282 325 Size: 1498 Fragments arranged by size: 3892 1498 1255 552 458 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3639 4509 6701 Size: 274 80 33 51 1443 1662 870 2192 Cuts at: 6701 8413 96 Size: 1712 138 Fragments arranged by size: 2192 1712 1662 1443 870 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3220 3252 3897 3929 8415 8447 3220 Size: 32 645 32 4486 32 3228 Fragments arranged by size: 4486 3228 645 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2296 5632 1316 Size: 980 3336 4139 Fragments arranged by size: 4139 3336 980 XbaI T'CTAG_A Cuts at: 5791 5791 Size: 8455 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 4180 4414 4459 5161 5738 2167 Size: 2013 234 45 702 577 4884 Fragments arranged by size: 4884 2013 702 577 234 45 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8455 XmaI C'CCGG_G Cuts at: 1870 2405 3599 5780 1870 Size: 535 1194 2181 4545 Fragments arranged by size: 4545 2181 1194 535 XmnI GAAnn'nnTTC Cuts at: 3377 5204 6584 3377 Size: 1827 1380 5248 Fragments arranged by size: 5248 1827 1380 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6263 276 Size: 53 83 186 936 4729 2468 Fragments arranged by size: 4729 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BfrBI BsiWI BstZ17I FalI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SrfI SwaI