(Circular) (Six-base) MAPSORT of: pFN21AA0675.seq Check: 3651 from: 1 to: 8329 pFN21AA0675 8329 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6139 278 Size: 53 83 186 936 4603 2468 Fragments arranged by size: 4603 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3705 4102 4731 5672 1937 Size: 1768 397 629 941 4594 Fragments arranged by size: 4594 1768 941 629 397 Acc65I G'GTAC_C Cuts at: 5650 5650 Size: 8329 AclI AA'CG_TT Cuts at: 1563 6456 6829 1563 Size: 4893 373 3063 Fragments arranged by size: 4893 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 6392 7776 8044 1632 Size: 4760 1384 268 1917 Fragments arranged by size: 4760 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8132 AflIII A'CryG_T Cuts at: 1203 5552 7228 8205 1203 Size: 4349 1676 977 1327 Fragments arranged by size: 4349 1676 1327 977 AgeI A'CCGG_T Cuts at: 7160 7160 Size: 8329 AhdI GACnn_n'nnGTC Cuts at: 7058 7058 Size: 8329 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8329 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4842 1184 Size: 3658 4671 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4142 4174 4142 Size: 32 8297 AlwNI CAG_nnn'CTG Cuts at: 2474 5045 6072 7644 2474 Size: 2571 1027 1572 3159 Fragments arranged by size: 3159 2571 1572 1027 ApaLI G'TGCA_C Cuts at: 6386 7542 6386 Size: 1156 7173 ApoI r'AATT_y Cuts at: 1493 2024 2483 2520 2606 2871 2893 3009 3055 Size: 531 459 37 86 265 22 116 46 Cuts at: 3055 3131 5507 5591 5638 5864 6016 8125 1493 Size: 76 2376 84 47 226 152 2109 1697 Fragments arranged by size: 2376 2109 1697 531 459 265 226 152 116 86 84 76 47 46 37 22 AseI AT'TA_AT Cuts at: 160 1032 2187 2391 3903 6883 160 Size: 872 1155 204 1512 2980 1606 Fragments arranged by size: 2980 1606 1512 1155 872 204 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8329 AvaI C'yCGr_G Cuts at: 1870 1943 4972 5654 1870 Size: 73 3029 682 4545 Fragments arranged by size: 4545 3029 682 73 AvrII C'CTAG_G Cuts at: 3709 3709 Size: 8329 BamHI G'GATC_C Cuts at: 5659 6026 5659 Size: 367 7962 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5650 7105 618 Size: 325 217 627 3863 1455 1842 Fragments arranged by size: 3863 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4208 5008 5035 5648 1391 Size: 2817 800 27 613 4072 Fragments arranged by size: 4072 2817 800 613 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4001 4035 4158 4912 928 Size: 971 2102 34 123 754 4345 Fragments arranged by size: 4345 2102 971 754 123 34 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6520 6554 1903 Size: 34 4583 34 3678 Fragments arranged by size: 4583 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2913 6221 7431 2913 Size: 3308 1210 3811 Fragments arranged by size: 3811 3308 1210 BclI T'GATC_A Cuts at: 1548 2917 1548 Size: 1369 6960 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4751 6940 136 Size: 107 122 71 631 605 3079 2189 1525 Fragments arranged by size: 3079 2189 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2701 3955 4496 8324 2701 Size: 1254 541 3828 2706 Fragments arranged by size: 3828 2706 1254 541 BlpI GC'TnA_GC Cuts at: 4207 5746 4207 Size: 1539 6790 Bme1580I G_kGCm'C Cuts at: 1790 6390 7546 1790 Size: 4600 1156 2573 Fragments arranged by size: 4600 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2558 1318 Size: 1240 7089 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3921 4866 5061 Size: 416 53 83 399 24 2504 945 195 Cuts at: 5061 5242 6060 7018 442 Size: 181 818 958 1753 Fragments arranged by size: 2504 1753 958 945 818 416 399 195 181 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8329 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2453 3694 4222 2453 Size: 1241 528 6560 Fragments arranged by size: 6560 1241 528 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3944 4830 5386 5457 6989 1239 Size: 2705 886 556 71 1532 2579 Fragments arranged by size: 2705 2579 1532 886 556 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4852 6454 7319 7617 4852 Size: 1602 865 298 5564 Fragments arranged by size: 5564 1602 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3569 6992 882 Size: 617 2070 3423 2219 Fragments arranged by size: 3423 2219 2070 617 BsaAI yAC'GTr Cuts at: 493 1204 4549 6120 493 Size: 711 3345 1571 2702 Fragments arranged by size: 3345 2702 1571 711 BsaBI GATnn'nnATC Cuts at: 2504 3690 8323 2504 Size: 1186 4633 2510 Fragments arranged by size: 4633 2510 1186 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6136 6518 7153 275 Size: 53 83 186 936 4603 382 635 1451 Fragments arranged by size: 4603 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6762 7160 7434 7581 7992 6762 Size: 398 274 147 411 7099 Fragments arranged by size: 7099 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4828 4858 5043 5073 1178 Size: 30 165 30 3425 30 185 30 4434 Fragments arranged by size: 4434 3425 185 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3249 4851 1186 Size: 2063 1602 4664 Fragments arranged by size: 4664 2063 1602 BseYI C'CCAG_C Cuts at: 5346 5606 7532 8028 5346 Size: 260 1926 496 5647 Fragments arranged by size: 5647 1926 496 260 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2860 3432 5091 5584 1163 Size: 573 1124 572 1659 493 3908 Fragments arranged by size: 3908 1659 1124 573 572 493 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5779 6540 7568 1540 Size: 275 186 3778 761 1028 2301 Fragments arranged by size: 3778 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2578 6390 6475 7546 2578 Size: 3812 85 1071 3361 Fragments arranged by size: 3812 3361 1071 85 BsmI GAATG_Cn' Cuts at: 4747 4775 5840 5933 4747 Size: 28 1065 93 7143 Fragments arranged by size: 7143 1065 93 28 BsmBI CGTCTCn'nnnn_ Cuts at: 4987 5046 4987 Size: 59 8270 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2578 4208 5008 5035 5648 6390 6475 Size: 399 788 1630 800 27 613 742 85 Cuts at: 6475 7546 1391 Size: 1071 2174 Fragments arranged by size: 2174 1630 1071 800 788 742 613 399 85 27 BspEI T'CCGG_A Cuts at: 7992 7992 Size: 8329 BspHI T'CATG_A Cuts at: 5097 5458 6218 7948 5097 Size: 361 760 1730 5478 Fragments arranged by size: 5478 1730 760 361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5063 5684 844 Size: 4219 621 3489 Fragments arranged by size: 4219 3489 621 BsrBI CCG'CTC Cuts at: 1394 1823 6216 1394 Size: 429 4393 3507 Fragments arranged by size: 4393 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2990 4152 6824 6998 65 Size: 1183 1742 1162 2672 174 1396 Fragments arranged by size: 2672 1742 1396 1183 1162 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5610 6973 7160 1700 Size: 110 3800 1363 187 2869 Fragments arranged by size: 3800 2869 1363 187 110 BsrGI T'GTAC_A Cuts at: 96 2957 96 Size: 2861 5468 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8329 BssSI C'ACGA_G Cuts at: 6389 7401 6389 Size: 1012 7317 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8329 BstBI TT'CG_AA Cuts at: 7133 7133 Size: 8329 BstEII G'GTnAC_C Cuts at: 1184 5600 1184 Size: 4416 3913 BstYI r'GATC_y Cuts at: 1972 2662 2701 3955 4496 5659 6026 6411 6428 Size: 690 39 1254 541 1163 367 385 17 Cuts at: 6428 7880 7966 7978 8324 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1254 1163 690 541 385 367 346 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 4781 4781 Size: 8329 BtgI C'CryG_G Cuts at: 513 1065 2004 5531 513 Size: 552 939 3527 3311 Fragments arranged by size: 3527 3311 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2584 487 Size: 683 56 1358 6232 Fragments arranged by size: 6232 1358 683 56 BtsI GCAGTG_nn' Cuts at: 5845 6639 6659 5845 Size: 794 20 7515 Fragments arranged by size: 7515 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8329 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2187 2222 529 Size: 35 1623 35 6636 Fragments arranged by size: 6636 1623 35 35 DraI TTT'AAA Cuts at: 2698 2898 3129 3435 5633 2698 Size: 200 231 306 2198 5394 Fragments arranged by size: 5394 2198 306 231 200 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7336 809 Size: 507 6020 1802 Fragments arranged by size: 6020 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4993 5776 6665 Size: 54 1425 50 275 18 3163 783 889 Cuts at: 6665 8294 8 Size: 1629 43 Fragments arranged by size: 3163 1629 1425 889 783 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5776 1537 Size: 275 3964 4090 Fragments arranged by size: 4090 3964 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3804 4053 5421 5433 6259 1353 Size: 2451 249 1368 12 826 3423 Fragments arranged by size: 3423 2451 1368 826 249 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6916 7290 7436 387 Size: 1064 138 5327 374 146 1280 Fragments arranged by size: 5327 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3944 4830 5386 5457 6392 6989 7776 Size: 393 2312 886 556 71 935 597 787 Cuts at: 7776 8044 1239 Size: 268 1524 Fragments arranged by size: 2312 1524 935 886 787 597 556 393 268 71 EcoO109I rG'GnC_Cy Cuts at: 3915 5387 3915 Size: 1472 6857 EcoRI G'AATT_C Cuts at: 5591 5638 6016 5591 Size: 47 378 7904 Fragments arranged by size: 7904 378 47 EcoRV GAT'ATC Cuts at: 3084 3535 3084 Size: 451 7878 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2245 2366 2723 3296 4499 2245 Size: 121 357 573 1203 6075 Fragments arranged by size: 6075 1203 573 357 121 FspI TGC'GCA Cuts at: 6835 6835 Size: 8329 HaeII r_GCGC'y Cuts at: 1678 5045 7476 1678 Size: 3367 2431 2531 Fragments arranged by size: 3367 2531 2431 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3105 3137 3316 3348 3547 Size: 32 135 32 1533 32 179 32 199 Cuts at: 3547 3579 3902 3934 5453 5485 6970 7002 7044 Size: 32 323 32 1519 32 1485 32 42 Cuts at: 7044 7076 1373 Size: 32 2626 Fragments arranged by size: 2626 1533 1519 1485 323 199 179 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2102 2149 5673 5919 669 Size: 988 281 164 47 3524 246 3079 Fragments arranged by size: 3524 3079 988 281 246 164 47 HindIII A'AGCT_T Cuts at: 748 2617 3164 748 Size: 1869 547 5913 Fragments arranged by size: 5913 1869 547 HpaI GTT'AAC Cuts at: 2149 5919 2149 Size: 3770 4559 KpnI G_GTAC'C Cuts at: 5654 5654 Size: 8329 MfeI C'AATT_G Cuts at: 5928 5928 Size: 8329 MluI A'CGCG_T Cuts at: 8205 8205 Size: 8329 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4718 5582 7443 7627 1305 Size: 3413 864 1861 184 2007 Fragments arranged by size: 3413 2007 1861 864 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4995 10 Size: 54 1425 343 3163 3344 Fragments arranged by size: 3344 3163 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3291 3435 6287 6646 6805 518 Size: 648 2125 144 2852 359 159 2042 Fragments arranged by size: 2852 2125 2042 648 359 159 144 MspA1I CmG'CkG Cuts at: 1606 4211 5745 6422 7570 7815 1606 Size: 2605 1534 677 1148 245 2120 Fragments arranged by size: 2605 2120 1534 1148 677 245 NaeI GCC'GGC Cuts at: 5612 5612 Size: 8329 NcoI C'CATG_G Cuts at: 513 1065 2004 5531 513 Size: 552 939 3527 3311 Fragments arranged by size: 3527 3311 939 552 NdeI CA'TA_TG Cuts at: 387 4042 387 Size: 3655 4674 NgoMIV G'CCGG_C Cuts at: 5610 5610 Size: 8329 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8329 NotI GC'GGCC_GC Cuts at: 5776 5776 Size: 8329 NspI r_CATG'y Cuts at: 1132 1229 5556 5687 7232 1132 Size: 97 4327 131 1545 2229 Fragments arranged by size: 4327 2229 1545 131 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8329 PciI A'CATG_T Cuts at: 5552 7228 5552 Size: 1676 6653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8329 PfoI T'CCnGG_A Cuts at: 3960 3960 Size: 8329 PmeI GTTT'AAAC Cuts at: 5633 5633 Size: 8329 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 6398 6430 7911 7943 3563 Size: 32 2803 32 1481 32 3949 Fragments arranged by size: 3949 2803 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3915 5387 3915 Size: 1472 6857 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8329 PsiI TTA'TAA Cuts at: 2817 3174 3425 4109 4278 5899 2817 Size: 357 251 684 169 1621 5247 Fragments arranged by size: 5247 1621 684 357 251 169 PstI C_TGCA'G Cuts at: 830 3505 4966 5681 830 Size: 2675 1461 715 3478 Fragments arranged by size: 3478 2675 1461 715 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8329 PvuII CAG'CTG Cuts at: 4211 4211 Size: 8329 SalI G'TCGA_C Cuts at: 1936 5671 1936 Size: 3735 4594 SapI GCTCTTCn'nnn_ Cuts at: 4053 5433 4053 Size: 1380 6949 SbfI CC_TGCA'GG Cuts at: 5681 5681 Size: 8329 ScaI AGT'ACT Cuts at: 6577 8289 6577 Size: 1712 6617 SfcI C'TryA_G Cuts at: 826 1045 2667 3501 3773 4162 4224 4962 5677 Size: 219 1622 834 272 389 62 738 715 Cuts at: 5677 6812 7493 7684 826 Size: 1135 681 191 1471 Fragments arranged by size: 1622 1471 1135 834 738 715 681 389 272 219 191 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8329 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8329 SmaI CCC'GGG Cuts at: 1872 4974 5656 1872 Size: 3102 682 4545 Fragments arranged by size: 4545 3102 682 SmlI C'TyrA_G Cuts at: 820 1017 1943 4831 6433 7334 7596 820 Size: 197 926 2888 1602 901 262 1553 Fragments arranged by size: 2888 1602 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4549 6120 493 Size: 4056 1571 2702 Fragments arranged by size: 4056 2702 1571 SpeI A'CTAG_T Cuts at: 152 152 Size: 8329 SphI G_CATG'C Cuts at: 1132 5687 1132 Size: 4555 3774 SspI AAT'ATT Cuts at: 5 52 2395 3237 6253 5 Size: 47 2343 842 3016 2081 Fragments arranged by size: 3016 2343 2081 842 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8329 StyI C'CwwG_G Cuts at: 513 1065 2004 3622 3709 5531 5768 513 Size: 552 939 1618 87 1822 237 3074 Fragments arranged by size: 3074 1822 1618 939 552 237 87 SwaI ATTT'AAAT Cuts at: 3435 3435 Size: 8329 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3130 3329 3466 4885 6356 6373 6526 Size: 1255 1550 199 137 1419 1471 17 153 Cuts at: 6526 6711 7156 325 Size: 185 445 1498 Fragments arranged by size: 1550 1498 1471 1419 1255 445 199 185 153 137 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2957 3855 4791 Size: 274 80 33 51 1443 980 898 936 Cuts at: 4791 6575 8287 96 Size: 1784 1712 138 Fragments arranged by size: 1784 1712 1443 980 936 898 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5043 5075 5274 5306 8289 8321 5043 Size: 32 199 32 2983 32 5051 Fragments arranged by size: 5051 2983 199 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4034 1316 Size: 2718 5611 XbaI T'CTAG_A Cuts at: 4636 5665 4636 Size: 1029 7300 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2393 3334 5361 2393 Size: 941 2027 5361 Fragments arranged by size: 5361 2027 941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8329 XmaI C'CCGG_G Cuts at: 1870 4972 5654 1870 Size: 3102 682 4545 Fragments arranged by size: 4545 3102 682 XmnI GAAnn'nnTTC Cuts at: 2301 2885 4710 6458 2301 Size: 584 1825 1748 4172 Fragments arranged by size: 4172 1825 1748 584 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6137 276 Size: 53 83 186 936 4603 2468 Fragments arranged by size: 4603 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI