(Circular) (Six-base) MAPSORT of: pFN21AA0596.seq Check: 1262 from: 1 to: 9247 pFN21AA0596 9247 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7057 278 Size: 53 83 186 936 5521 2468 Fragments arranged by size: 5521 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3195 4869 6590 1937 Size: 1258 1674 1721 4594 Fragments arranged by size: 4594 1721 1674 1258 Acc65I G'GTAC_C Cuts at: 6568 6568 Size: 9247 AclI AA'CG_TT Cuts at: 1563 7374 7747 1563 Size: 5811 373 3063 Fragments arranged by size: 5811 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3512 3569 4413 5070 6375 7310 8694 8962 Size: 1880 57 844 657 1305 935 1384 268 Cuts at: 8962 1632 Size: 1917 Fragments arranged by size: 1917 1880 1384 1305 935 844 657 268 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9050 AflIII A'CryG_T Cuts at: 1203 2487 8146 9123 1203 Size: 1284 5659 977 1327 Fragments arranged by size: 5659 1327 1284 977 AgeI A'CCGG_T Cuts at: 8078 8078 Size: 9247 AhdI GACnn_n'nnGTC Cuts at: 7976 7976 Size: 9247 AleI CACnn'nnGTG Cuts at: 1166 2711 1166 Size: 1545 7702 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2361 1184 Size: 1177 8070 AlwNI CAG_nnn'CTG Cuts at: 2153 3079 3382 3535 3691 4234 4538 4998 5022 Size: 926 303 153 156 543 304 460 24 Cuts at: 5022 5498 5538 6108 6121 6406 6484 6990 8562 Size: 476 40 570 13 285 78 506 1572 Cuts at: 8562 2153 Size: 2838 Fragments arranged by size: 2838 1572 926 570 543 506 476 460 304 303 285 156 153 78 40 24 13 ApaI G_GGCC'C Cuts at: 5159 5257 5968 6099 5159 Size: 98 711 131 8307 Fragments arranged by size: 8307 711 131 98 ApaLI G'TGCA_C Cuts at: 3518 5600 7304 8460 3518 Size: 2082 1704 1156 4305 Fragments arranged by size: 4305 2082 1704 1156 ApoI r'AATT_y Cuts at: 1493 2606 4133 6556 6782 6934 9043 1493 Size: 1113 1527 2423 226 152 2109 1697 Fragments arranged by size: 2423 2109 1697 1527 1113 226 152 AseI AT'TA_AT Cuts at: 160 1032 7801 160 Size: 872 6769 1606 Fragments arranged by size: 6769 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9247 AvaI C'yCGr_G Cuts at: 1870 1943 3200 4932 5670 6572 1870 Size: 73 1257 1732 738 902 4545 Fragments arranged by size: 4545 1732 1257 902 738 73 AvrII C'CTAG_G Cuts at: 6004 6068 6004 Size: 64 9183 BamHI G'GATC_C Cuts at: 3433 6577 6944 3433 Size: 3144 367 5736 Fragments arranged by size: 5736 3144 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2903 4194 4314 5389 5426 Size: 325 217 627 1116 1291 120 1075 37 Cuts at: 5426 5611 6053 6568 8023 618 Size: 185 442 515 1455 1842 Fragments arranged by size: 1842 1455 1291 1116 1075 627 515 442 325 217 185 120 37 BanII G_rGCy'C Cuts at: 1391 3296 3882 4205 4973 5073 5159 5257 5545 Size: 1905 586 323 768 100 86 98 288 Cuts at: 5545 5702 5887 5968 6099 6282 6566 1391 Size: 157 185 81 131 183 284 4072 Fragments arranged by size: 4072 1905 768 586 323 288 284 185 183 157 131 100 98 86 81 BbeI G_GCGC'C Cuts at: 4198 4198 Size: 9247 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3857 5058 6185 928 Size: 971 1958 1201 1127 3990 Fragments arranged by size: 3990 1958 1201 1127 971 BbvCI CC'TCA_GC Cuts at: 2108 2819 3332 5115 6343 2108 Size: 711 513 1783 1228 5012 Fragments arranged by size: 5012 1783 1228 711 513 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4431 4465 5355 5389 7438 7472 1903 Size: 34 2494 34 890 34 2049 34 3678 Fragments arranged by size: 3678 2494 2049 890 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3045 4894 7139 8349 3045 Size: 1849 2245 1210 3943 Fragments arranged by size: 3943 2245 1849 1210 BclI T'GATC_A Cuts at: 1548 3756 1548 Size: 2208 7039 BfrBI ATG'CAT Cuts at: 3422 3422 Size: 9247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4756 7858 136 Size: 107 122 71 631 605 3084 3102 1525 Fragments arranged by size: 3102 3084 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2054 4834 9242 2054 Size: 2780 4408 2059 Fragments arranged by size: 4408 2780 2059 BlpI GC'TnA_GC Cuts at: 6664 6664 Size: 9247 Bme1580I G_kGCm'C Cuts at: 1790 2340 2658 3509 3522 4507 5159 5257 5604 Size: 550 318 851 13 985 652 98 347 Cuts at: 5604 5968 6056 6099 6115 7308 8464 1790 Size: 364 88 43 16 1193 1156 2573 Fragments arranged by size: 2573 1193 1156 985 851 652 550 364 347 318 98 88 43 16 13 BmgBI CAC'GTC Cuts at: 1318 2365 2760 1318 Size: 1047 395 7805 Fragments arranged by size: 7805 1047 395 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3231 4498 5252 Size: 416 53 83 399 24 1814 1267 754 Cuts at: 5252 5587 6978 7936 442 Size: 335 1391 958 1753 Fragments arranged by size: 1814 1753 1391 1267 958 754 416 399 335 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2988 3623 1056 Size: 1932 635 6680 Fragments arranged by size: 6680 1932 635 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2123 6514 2123 Size: 4391 4856 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2175 2346 3036 3202 3423 3605 3828 4911 Size: 936 171 690 166 221 182 223 1083 Cuts at: 4911 5175 5232 5460 5567 6272 6509 7907 1239 Size: 264 57 228 107 705 237 1398 2579 Fragments arranged by size: 2579 1398 1083 936 705 690 264 237 228 223 221 182 171 166 107 57 Bpu10I CC'TnA_GC Cuts at: 2108 2671 2819 2942 3332 4199 5115 5685 6221 Size: 563 148 123 390 867 916 570 536 Cuts at: 6221 6343 2108 Size: 122 5012 Fragments arranged by size: 5012 916 867 570 563 536 390 148 123 122 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4739 5341 5811 7372 8237 8535 4739 Size: 602 470 1561 865 298 5451 Fragments arranged by size: 5451 1561 865 602 470 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5371 7910 882 Size: 617 3872 2539 2219 Fragments arranged by size: 3872 2539 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3841 7038 493 Size: 711 2637 3197 2702 Fragments arranged by size: 3197 2702 2637 711 BsaBI GATnn'nnATC Cuts at: 2059 4214 9241 2059 Size: 2155 5027 2065 Fragments arranged by size: 5027 2155 2065 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4195 7054 7436 8071 Size: 53 83 186 936 2662 2859 382 635 Cuts at: 8071 275 Size: 1451 Fragments arranged by size: 2859 2662 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7680 8078 8352 8499 8910 7680 Size: 398 274 147 411 8017 Fragments arranged by size: 8017 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 2390 2420 3692 Size: 30 165 30 693 30 264 30 1272 Cuts at: 3692 3722 3801 3831 1178 Size: 30 79 30 6594 Fragments arranged by size: 6594 1272 693 264 165 79 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3247 3420 4066 5083 5156 5344 6362 1186 Size: 2061 173 646 1017 73 188 1018 4071 Fragments arranged by size: 4071 2061 1018 1017 646 188 173 73 BseYI C'CCAG_C Cuts at: 2137 2203 2662 2680 3881 4457 4462 4531 4572 Size: 66 459 18 1201 576 5 69 41 Cuts at: 4572 5477 5484 5538 5710 5876 6098 6114 6366 Size: 905 7 54 172 166 222 16 252 Cuts at: 6366 6419 8450 8946 2137 Size: 53 2031 496 2438 Fragments arranged by size: 2438 2031 1201 905 576 496 459 252 222 172 166 69 66 54 53 41 18 16 7 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2918 3839 4025 4830 4940 5282 1163 Size: 573 1182 921 186 805 110 342 5128 Fragments arranged by size: 5128 1182 921 805 573 342 186 110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6697 7458 8486 1540 Size: 275 186 4696 761 1028 2301 Fragments arranged by size: 4696 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2376 2403 3296 3522 4205 4973 5043 5066 5446 Size: 27 893 226 683 768 70 23 380 Cuts at: 5446 5604 6282 7308 7393 8464 2376 Size: 158 678 1026 85 1071 3159 Fragments arranged by size: 3159 1071 1026 893 768 683 678 380 226 158 85 70 27 23 BsmI GAATG_Cn' Cuts at: 2650 3031 5939 6203 6758 6851 2650 Size: 381 2908 264 555 93 5046 Fragments arranged by size: 5046 2908 555 381 264 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5525 5733 5525 Size: 208 9039 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2340 2376 2403 2658 3296 3509 3522 Size: 399 550 36 27 255 638 213 13 Cuts at: 3522 3882 4205 4507 4973 5043 5066 5073 5159 Size: 360 323 302 466 70 23 7 86 Cuts at: 5159 5257 5446 5545 5604 5702 5887 5968 6056 Size: 98 189 99 59 98 185 81 88 Cuts at: 6056 6099 6115 6282 6566 7308 7393 8464 1391 Size: 43 16 167 284 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 638 550 466 399 360 323 302 284 255 213 189 185 167 99 98 98 88 86 85 81 70 59 43 36 27 23 16 13 7 BspEI T'CCGG_A Cuts at: 8910 8910 Size: 9247 BspHI T'CATG_A Cuts at: 7136 8866 7136 Size: 1730 7517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2940 3260 4852 5254 6602 844 Size: 2096 320 1592 402 1348 3489 Fragments arranged by size: 3489 2096 1592 1348 402 320 BsrBI CCG'CTC Cuts at: 1394 1823 2670 3453 7134 1394 Size: 429 847 783 3681 3507 Fragments arranged by size: 3681 3507 847 783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2979 3045 4029 5428 7742 7916 65 Size: 1183 1731 66 984 1399 2314 174 1396 Fragments arranged by size: 2314 1731 1399 1396 1183 984 174 66 BsrFI r'CCGG_y Cuts at: 1700 1810 3639 4311 4345 7891 8078 1700 Size: 110 1829 672 34 3546 187 2869 Fragments arranged by size: 3546 2869 1829 672 187 110 34 BsrGI T'GTAC_A Cuts at: 96 3090 96 Size: 2994 6253 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9247 BssSI C'ACGA_G Cuts at: 7307 8319 7307 Size: 1012 8235 BstAPI GCAn_nnn'nTGC Cuts at: 794 5022 794 Size: 4228 5019 BstBI TT'CG_AA Cuts at: 8051 8051 Size: 9247 BstEII G'GTnAC_C Cuts at: 1184 2119 1184 Size: 935 8312 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3139 5554 5767 6193 2011 Size: 1128 2415 213 426 5065 Fragments arranged by size: 5065 2415 1128 426 213 BstYI r'GATC_y Cuts at: 1972 2054 3433 4023 4604 4615 4834 6577 6944 Size: 82 1379 590 581 11 219 1743 367 Cuts at: 6944 7329 7346 8798 8884 8896 9242 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1743 1452 1379 590 581 385 367 346 219 86 82 17 12 11 Bsu36I CC'TnA_GG Cuts at: 2764 4018 4078 4761 2764 Size: 1254 60 683 7250 Fragments arranged by size: 7250 1254 683 60 BtgI C'CryG_G Cuts at: 513 1065 2004 4515 4896 5766 6192 6283 6519 Size: 552 939 2511 381 870 426 91 236 Cuts at: 6519 513 Size: 3241 Fragments arranged by size: 3241 2511 939 870 552 426 381 236 91 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8508 Fragments arranged by size: 8508 683 56 BtsI GCAGTG_nn' Cuts at: 2283 2556 2583 2854 3343 3714 3792 3949 4409 Size: 273 27 271 489 371 78 157 460 Cuts at: 4409 5660 5912 6263 6309 6432 6763 7557 7577 Size: 1251 252 351 46 123 331 794 20 Cuts at: 7577 2283 Size: 3953 Fragments arranged by size: 3953 1251 794 489 460 371 351 331 273 271 252 157 123 78 46 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9247 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2829 2864 4084 4119 529 Size: 35 2265 35 1220 35 5657 Fragments arranged by size: 5657 2265 1220 35 35 35 DraI TTT'AAA Cuts at: 3967 6551 3967 Size: 2584 6663 DraIII CAC_nnn'GTG Cuts at: 3841 3844 3980 6322 3841 Size: 3 136 2342 6766 Fragments arranged by size: 6766 2342 136 3 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2480 2551 8254 809 Size: 507 1164 71 5703 1802 Fragments arranged by size: 5703 1802 1164 507 71 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2385 4092 4105 Size: 54 1425 50 275 18 555 1707 13 Cuts at: 4105 4236 4674 5232 5556 5769 6694 7583 9212 Size: 131 438 558 324 213 925 889 1629 Cuts at: 9212 8 Size: 43 Fragments arranged by size: 1707 1629 1425 925 889 558 555 438 324 275 213 131 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 6694 1537 Size: 275 4882 4090 Fragments arranged by size: 4882 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3014 4558 4942 5100 5124 5133 5302 6143 Size: 1661 1544 384 158 24 9 169 841 Cuts at: 6143 6394 7177 1353 Size: 251 783 3423 Fragments arranged by size: 3423 1661 1544 841 783 384 251 169 158 24 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2753 3263 7834 8208 8354 387 Size: 1064 138 1164 510 4571 374 146 1280 Fragments arranged by size: 4571 1280 1164 1064 510 374 146 138 EcoICRI GAG'CTC Cuts at: 3294 4203 4971 6280 3294 Size: 909 768 1309 6261 Fragments arranged by size: 6261 1309 909 768 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2175 2346 3036 3202 3423 3512 3569 Size: 393 543 171 690 166 221 89 57 Cuts at: 3569 3605 3828 4413 4911 5070 5175 5232 5460 Size: 36 223 585 498 159 105 57 228 Cuts at: 5460 5567 6272 6375 6509 7310 7907 8694 8962 Size: 107 705 103 134 801 597 787 268 Cuts at: 8962 1239 Size: 1524 Fragments arranged by size: 1524 801 787 705 690 597 585 543 498 393 268 228 223 221 171 166 159 134 107 105 103 89 57 57 36 EcoNI CCTnn'n_nnAGG Cuts at: 5652 6224 5652 Size: 572 8675 EcoO109I rG'GnC_Cy Cuts at: 2104 3127 3379 4321 4553 4654 4714 5156 5253 Size: 1023 252 942 232 101 60 442 97 Cuts at: 5253 5254 5266 5644 5965 6146 6481 2104 Size: 1 12 378 321 181 335 4870 Fragments arranged by size: 4870 1023 942 442 378 335 321 252 232 181 101 97 60 12 1 EcoRI G'AATT_C Cuts at: 6556 6934 6556 Size: 378 8869 EcoRV GAT'ATC Cuts at: 5778 5778 Size: 9247 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2140 4418 6007 2140 Size: 2278 1589 5380 Fragments arranged by size: 5380 2278 1589 FspI TGC'GCA Cuts at: 2075 3798 7753 2075 Size: 1723 3955 3569 Fragments arranged by size: 3955 3569 1723 HaeII r_GCGC'y Cuts at: 1678 2442 4198 4573 8394 1678 Size: 764 1756 375 3821 2531 Fragments arranged by size: 3821 2531 1756 764 375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2029 2061 2096 2128 2168 Size: 32 135 32 457 32 35 32 40 Cuts at: 2168 2200 3046 3078 3686 3718 4061 4093 4216 Size: 32 846 32 608 32 343 32 123 Cuts at: 4216 4248 4335 4367 5075 5107 5505 5537 5668 Size: 32 87 32 708 32 398 32 131 Cuts at: 5668 5700 6371 6403 7888 7920 7962 7994 1373 Size: 32 671 32 1485 32 42 32 2626 Fragments arranged by size: 2626 1485 846 708 671 608 457 398 343 135 131 123 87 42 40 35 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2022 2485 6591 6837 669 Size: 988 281 84 463 4106 246 3079 Fragments arranged by size: 4106 3079 988 463 281 246 84 HindIII A'AGCT_T Cuts at: 748 748 Size: 9247 HpaI GTT'AAC Cuts at: 6837 6837 Size: 9247 KasI G'GCGC_C Cuts at: 4194 4194 Size: 9247 KpnI G_GTAC'C Cuts at: 6572 6572 Size: 9247 MfeI C'AATT_G Cuts at: 6846 6846 Size: 9247 MluI A'CGCG_T Cuts at: 9123 9123 Size: 9247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2547 3411 6338 8361 8545 1305 Size: 1242 864 2927 2023 184 2007 Fragments arranged by size: 2927 2023 2007 1242 864 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4094 4107 4676 5234 5558 Size: 54 1425 343 2262 13 569 558 324 Cuts at: 5558 5771 10 Size: 213 3486 Fragments arranged by size: 3486 2262 1425 569 558 343 324 213 54 13 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2406 2513 2711 2864 3359 3872 Size: 648 843 397 107 198 153 495 513 Cuts at: 3872 4001 4127 4163 4187 4220 7205 7564 7723 Size: 129 126 36 24 33 2985 359 159 Cuts at: 7723 518 Size: 2042 Fragments arranged by size: 2985 2042 843 648 513 495 397 359 198 159 153 129 126 107 36 33 24 MspA1I CmG'CkG Cuts at: 1606 3762 3885 4041 4179 4258 4627 5022 5114 Size: 2156 123 156 138 79 369 395 92 Cuts at: 5114 5538 5714 6242 6268 6285 6663 7340 8488 Size: 424 176 528 26 17 378 677 1148 Cuts at: 8488 8733 1606 Size: 245 2120 Fragments arranged by size: 2156 2120 1148 677 528 424 395 378 369 245 176 156 138 123 92 79 26 17 NarI GG'CG_CC Cuts at: 4195 4195 Size: 9247 NcoI C'CATG_G Cuts at: 513 1065 2004 4896 5766 6192 513 Size: 552 939 2892 870 426 3568 Fragments arranged by size: 3568 2892 939 870 552 426 NdeI CA'TA_TG Cuts at: 387 387 Size: 9247 NheI G'CTAG_C Cuts at: 1052 2984 3619 1052 Size: 1932 635 6680 Fragments arranged by size: 6680 1932 635 NotI GC'GGCC_GC Cuts at: 6694 6694 Size: 9247 NsiI A_TGCA'T Cuts at: 3424 3424 Size: 9247 NspI r_CATG'y Cuts at: 1132 1229 2344 6605 8150 1132 Size: 97 1115 4261 1545 2229 Fragments arranged by size: 4261 2229 1545 1115 97 PasI CC'CwG_GG Cuts at: 1348 6452 1348 Size: 5104 4143 PciI A'CATG_T Cuts at: 8146 8146 Size: 9247 PflMI CCAn_nnn'nTGG Cuts at: 1108 2968 3655 4837 5717 6322 1108 Size: 1860 687 1182 880 605 4033 Fragments arranged by size: 4033 1860 1182 880 687 605 PfoI T'CCnGG_A Cuts at: 2270 4339 4524 5895 2270 Size: 2069 185 1371 5622 Fragments arranged by size: 5622 2069 1371 185 PmeI GTTT'AAAC Cuts at: 6551 6551 Size: 9247 PmlI CAC'GTG Cuts at: 3841 3841 Size: 9247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6528 6560 7316 7348 8829 8861 6528 Size: 32 756 32 1481 32 6914 Fragments arranged by size: 6914 1481 756 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 3127 4553 4654 5266 2104 Size: 1023 1426 101 612 6085 Fragments arranged by size: 6085 1426 1023 612 101 PshAI GACnn'nnGTC Cuts at: 1613 3606 1613 Size: 1993 7254 PsiI TTA'TAA Cuts at: 6817 6817 Size: 9247 PspOMI G'GGCC_C Cuts at: 5155 5253 5964 6095 5155 Size: 98 711 131 8307 Fragments arranged by size: 8307 711 131 98 PstI C_TGCA'G Cuts at: 830 2397 2590 4543 4653 5643 6599 830 Size: 1567 193 1953 110 990 956 3478 Fragments arranged by size: 3478 1953 1567 990 956 193 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9247 PvuII CAG'CTG Cuts at: 3762 3885 4041 4179 4627 5022 5114 5538 5714 Size: 123 156 138 448 395 92 424 176 Cuts at: 5714 6268 3762 Size: 554 6741 Fragments arranged by size: 6741 554 448 424 395 176 156 138 123 92 SacI G_AGCT'C Cuts at: 3296 4205 4973 6282 3296 Size: 909 768 1309 6261 Fragments arranged by size: 6261 1309 909 768 SacII CC_GC'GG Cuts at: 6286 6286 Size: 9247 SalI G'TCGA_C Cuts at: 1936 6589 1936 Size: 4653 4594 SanDI GG'GwC_CC Cuts at: 3127 3127 Size: 9247 SapI GCTCTTCn'nnn_ Cuts at: 5100 5100 Size: 9247 SbfI CC_TGCA'GG Cuts at: 4653 6599 4653 Size: 1946 7301 ScaI AGT'ACT Cuts at: 2315 7495 9207 2315 Size: 5180 1712 2355 Fragments arranged by size: 5180 2355 1712 SexAI A'CCwGG_T Cuts at: 5889 5889 Size: 9247 SfcI C'TryA_G Cuts at: 826 1045 2393 2586 3680 4539 4649 5168 5639 Size: 219 1348 193 1094 859 110 519 471 Cuts at: 5639 6595 7730 8411 8602 826 Size: 956 1135 681 191 1471 Fragments arranged by size: 1471 1348 1135 1094 956 859 681 519 471 219 193 191 110 SfoI GGC'GCC Cuts at: 4196 4196 Size: 9247 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9247 SmaI CCC'GGG Cuts at: 1872 4934 6574 1872 Size: 3062 1640 4545 Fragments arranged by size: 4545 3062 1640 SmlI C'TyrA_G Cuts at: 820 1017 1943 4718 5356 5826 7351 8252 8514 Size: 197 926 2775 638 470 1525 901 262 Cuts at: 8514 820 Size: 1553 Fragments arranged by size: 2775 1553 1525 926 901 638 470 262 197 SnaBI TAC'GTA Cuts at: 493 7038 493 Size: 6545 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9247 SphI G_CATG'C Cuts at: 1132 6605 1132 Size: 5473 3774 SspI AAT'ATT Cuts at: 5 52 3922 7171 5 Size: 47 3870 3249 2081 Fragments arranged by size: 3870 3249 2081 47 StuI AGG'CCT Cuts at: 1517 3224 1517 Size: 1707 7540 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 2124 4896 5766 6004 6068 Size: 552 939 111 9 2772 870 238 64 Cuts at: 6068 6142 6192 6686 513 Size: 74 50 494 3074 Fragments arranged by size: 3074 2772 939 870 552 494 238 111 74 64 50 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2800 3389 3930 4441 4864 7274 7291 Size: 1255 1220 589 541 511 423 2410 17 Cuts at: 7291 7444 7629 8074 325 Size: 153 185 445 1498 Fragments arranged by size: 2410 1498 1255 1220 589 541 511 445 423 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2313 2445 3090 Size: 274 80 33 51 1443 336 132 645 Cuts at: 3090 3150 3672 3891 5317 6020 7493 9205 96 Size: 60 522 219 1426 703 1473 1712 138 Fragments arranged by size: 1712 1473 1443 1426 703 645 522 336 274 219 138 132 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2133 2165 3692 3724 3801 3833 4401 4433 9207 Size: 32 1527 32 77 32 568 32 4774 Cuts at: 9207 9239 2133 Size: 32 2141 Fragments arranged by size: 4774 2141 1527 568 77 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9247 XbaI T'CTAG_A Cuts at: 6583 6583 Size: 9247 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2122 4298 4441 5764 6089 6323 2122 Size: 2176 143 1323 325 234 5046 Fragments arranged by size: 5046 2176 1323 325 234 143 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9247 XmaI C'CCGG_G Cuts at: 1870 4932 6572 1870 Size: 3062 1640 4545 Fragments arranged by size: 4545 3062 1640 XmnI GAAnn'nnTTC Cuts at: 4401 7376 4401 Size: 2975 6272 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7055 276 Size: 53 83 186 936 5521 2468 Fragments arranged by size: 5521 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BsiWI BstZ17I FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII SfiI SrfI SwaI