Restriction map for
pFN21AA0596
(Linear) (Six-base) MAPSORT of: KIAA0596.seq Check: 300 from: 1 to: 4542
KIAA0596.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 1190 2864 4542
Size: 1190 1674 1678
Fragments arranged by size:
1678 1674 1190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1507 1564 2408 3065 4370 4542
Size: 1507 57 844 657 1305 172
Fragments arranged by size:
1507 1305 844 657 172 57
AflIII A'CryG_T
Cuts at: 0 482 4542
Size: 482 4060
AleI CACnn'nnGTG
Cuts at: 0 706 4542
Size: 706 3836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 356 4542
Size: 356 4186
AlwNI CAG_nnn'CTG
Cuts at: 0 148 1074 1377 1530 1686 2229 2533 2993
Size: 148 926 303 153 156 543 304 460
Cuts at: 2993 3017 3493 3533 4103 4116 4401 4479 4542
Size: 24 476 40 570 13 285 78 63
Fragments arranged by size:
926 570 543 476 460 304 303 285
156 153 148 78 63 40 24 13
ApaI G_GGCC'C
Cuts at: 0 3154 3252 3963 4094 4542
Size: 3154 98 711 131 448
Fragments arranged by size:
3154 711 448 131 98
ApaLI G'TGCA_C
Cuts at: 0 1513 3595 4542
Size: 1513 2082 947
Fragments arranged by size:
2082 1513 947
ApoI r'AATT_y
Cuts at: 0 601 2128 4542
Size: 601 1527 2414
Fragments arranged by size:
2414 1527 601
AvaI C'yCGr_G
Cuts at: 0 1195 2927 3665 4542
Size: 1195 1732 738 877
Fragments arranged by size:
1732 1195 877 738
AvrII C'CTAG_G
Cuts at: 0 3999 4063 4542
Size: 3999 64 479
Fragments arranged by size:
3999 479 64
BamHI G'GATC_C
Cuts at: 0 1428 4542
Size: 1428 3114
BanI G'GyrC_C
Cuts at: 0 898 2189 2309 3384 3421 3606 4048 4542
Size: 898 1291 120 1075 37 185 442 494
Fragments arranged by size:
1291 1075 898 494 442 185 120 37
BanII G_rGCy'C
Cuts at: 0 1291 1877 2200 2968 3068 3154 3252 3540
Size: 1291 586 323 768 100 86 98 288
Cuts at: 3540 3697 3882 3963 4094 4277 4542
Size: 157 185 81 131 183 265
Fragments arranged by size:
1291 768 586 323 288 265 185 183
157 131 100 98 86 81
BbeI G_GCGC'C
Cuts at: 0 2193 4542
Size: 2193 2349
BbsI GAAGACnn'nnnn_
Cuts at: 0 1852 3053 4180 4542
Size: 1852 1201 1127 362
Fragments arranged by size:
1852 1201 1127 362
BbvCI CC'TCA_GC
Cuts at: 0 103 814 1327 3110 4338 4542
Size: 103 711 513 1783 1228 204
Fragments arranged by size:
1783 1228 711 513 204 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2426 2460 3350 3384 4542
Size: 2426 34 890 34 1158
Fragments arranged by size:
2426 1158 890 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1040 2889 4542
Size: 1040 1849 1653
Fragments arranged by size:
1849 1653 1040
BclI T'GATC_A
Cuts at: 0 1751 4542
Size: 1751 2791
BfrBI ATG'CAT
Cuts at: 0 1417 4542
Size: 1417 3125
BglI GCCn_nnn'nGGC
Cuts at: 0 2751 4542
Size: 2751 1791
BglII A'GATC_T
Cuts at: 0 49 2829 4542
Size: 49 2780 1713
Fragments arranged by size:
2780 1713 49
Bme1580I G_kGCm'C
Cuts at: 0 335 653 1504 1517 2502 3154 3252 3599
Size: 335 318 851 13 985 652 98 347
Cuts at: 3599 3963 4051 4094 4110 4542
Size: 364 88 43 16 432
Fragments arranged by size:
985 851 652 432 364 347 335 318
98 88 43 16 13
BmgBI CAC'GTC
Cuts at: 0 360 755 4542
Size: 360 395 3787
Fragments arranged by size:
3787 395 360
BmrI ACTGGGnnnn_n'
Cuts at: 0 1226 2493 3247 3582 4542
Size: 1226 1267 754 335 960
Fragments arranged by size:
1267 1226 960 754 335
BmtI G_CTAG'C
Cuts at: 0 983 1618 4542
Size: 983 635 2924
Fragments arranged by size:
2924 983 635
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 118 4509 4542
Size: 118 4391 33
Fragments arranged by size:
4391 118 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 341 1031 1197 1418 1600 1823 2906
Size: 170 171 690 166 221 182 223 1083
Cuts at: 2906 3170 3227 3455 3562 4267 4504 4542
Size: 264 57 228 107 705 237 38
Fragments arranged by size:
1083 705 690 264 237 228 223 221
182 171 170 166 107 57 38
Bpu10I CC'TnA_GC
Cuts at: 0 103 666 814 937 1327 2194 3110 3680
Size: 103 563 148 123 390 867 916 570
Cuts at: 3680 4216 4338 4542
Size: 536 122 204
Fragments arranged by size:
916 867 570 563 536 390 204 148
123 122 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2734 3336 3806 4542
Size: 2734 602 470 736
Fragments arranged by size:
2734 736 602 470
BsaI GGTCTCn'nnnn_
Cuts at: 0 3366 4542
Size: 3366 1176
BsaAI yAC'GTr
Cuts at: 0 1836 4542
Size: 1836 2706
BsaBI GATnn'nnATC
Cuts at: 0 54 2209 4542
Size: 54 2155 2333
Fragments arranged by size:
2333 2155 54
BsaHI Gr'CG_yC
Cuts at: 0 2190 4542
Size: 2190 2352
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 385 415 1687 1717 1796 1826
Size: 91 30 264 30 1272 30 79 30
Cuts at: 1826 4542
Size: 2716
Fragments arranged by size:
2716 1272 264 91 79 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1242 1415 2061 3078 3151 3339 4357 4542
Size: 1242 173 646 1017 73 188 1018 185
Fragments arranged by size:
1242 1018 1017 646 188 185 173 73
BseYI C'CCAG_C
Cuts at: 0 132 198 657 675 1876 2452 2457 2526
Size: 132 66 459 18 1201 576 5 69
Cuts at: 2526 2567 3472 3479 3533 3705 3871 4093 4109
Size: 41 905 7 54 172 166 222 16
Cuts at: 4109 4361 4414 4542
Size: 252 53 128
Fragments arranged by size:
1201 905 576 459 252 222 172 166
132 128 69 66 54 53 41 18
16 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1834 2020 2825 2935 3277 4542
Size: 913 921 186 805 110 342 1265
Fragments arranged by size:
1265 921 913 805 342 186 110
BsiHKAI G_wGCw'C
Cuts at: 0 371 398 1291 1517 2200 2968 3038 3061
Size: 371 27 893 226 683 768 70 23
Cuts at: 3061 3441 3599 4277 4542
Size: 380 158 678 265
Fragments arranged by size:
893 768 683 678 380 371 265 226
158 70 27 23
BsmI GAATG_Cn'
Cuts at: 0 645 1026 3934 4198 4542
Size: 645 381 2908 264 344
Fragments arranged by size:
2908 645 381 344 264
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3520 3728 4542
Size: 3520 208 814
Fragments arranged by size:
3520 814 208
Bsp1286I G_dGCh'C
Cuts at: 0 335 371 398 653 1291 1504 1517 1877
Size: 335 36 27 255 638 213 13 360
Cuts at: 1877 2200 2502 2968 3038 3061 3068 3154 3252
Size: 323 302 466 70 23 7 86 98
Cuts at: 3252 3441 3540 3599 3697 3882 3963 4051 4094
Size: 189 99 59 98 185 81 88 43
Cuts at: 4094 4110 4277 4542
Size: 16 167 265
Fragments arranged by size:
638 466 360 335 323 302 265 255
213 189 185 167 99 98 98 88
86 81 70 59 43 36 27 23
16 13 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 935 1255 2847 3249 4542
Size: 935 320 1592 402 1293
Fragments arranged by size:
1592 1293 935 402 320
BsrBI CCG'CTC
Cuts at: 0 665 1448 4542
Size: 665 783 3094
Fragments arranged by size:
3094 783 665
BsrDI GCAATG_nn'
Cuts at: 0 974 1040 2024 3423 4542
Size: 974 66 984 1399 1119
Fragments arranged by size:
1399 1119 984 974 66
BsrFI r'CCGG_y
Cuts at: 0 1634 2306 2340 4542
Size: 1634 672 34 2202
Fragments arranged by size:
2202 1634 672 34
BsrGI T'GTAC_A
Cuts at: 0 1085 4542
Size: 1085 3457
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3017 4542
Size: 3017 1525
BstEII G'GTnAC_C
Cuts at: 0 114 4542
Size: 114 4428
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1134 3549 3762 4188 4542
Size: 1134 2415 213 426 354
Fragments arranged by size:
2415 1134 426 354 213
BstYI r'GATC_y
Cuts at: 0 49 1428 2018 2599 2610 2829 4542
Size: 49 1379 590 581 11 219 1713
Fragments arranged by size:
1713 1379 590 581 219 49 11
Bsu36I CC'TnA_GG
Cuts at: 0 759 2013 2073 2756 4542
Size: 759 1254 60 683 1786
Fragments arranged by size:
1786 1254 759 683 60
BtgI C'CryG_G
Cuts at: 0 2510 2891 3761 4187 4278 4514 4542
Size: 2510 381 870 426 91 236 28
Fragments arranged by size:
2510 870 426 381 236 91 28
BtsI GCAGTG_nn'
Cuts at: 0 278 551 578 849 1338 1709 1787 1944
Size: 278 273 27 271 489 371 78 157
Cuts at: 1944 2404 3655 3907 4258 4304 4427 4542
Size: 460 1251 252 351 46 123 115
Fragments arranged by size:
1251 489 460 371 351 278 273 271
252 157 123 115 78 46 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 824 859 2079 2114 4542
Size: 824 35 1220 35 2428
Fragments arranged by size:
2428 1220 824 35 35
DraI TTT'AAA
Cuts at: 0 1962 4542
Size: 1962 2580
DraIII CAC_nnn'GTG
Cuts at: 0 1836 1839 1975 4317 4542
Size: 1836 3 136 2342 225
Fragments arranged by size:
2342 1836 225 136 3
DrdI GACnn_nn'nnGTC
Cuts at: 0 475 546 4542
Size: 475 71 3996
Fragments arranged by size:
3996 475 71
EaeI y'GGCC_r
Cuts at: 0 380 2087 2100 2231 2669 3227 3551 3764
Size: 380 1707 13 131 438 558 324 213
Cuts at: 3764 4542
Size: 778
Fragments arranged by size:
1707 778 558 438 380 324 213 131
13
EarI CTCTTCn'nnn_
Cuts at: 0 1009 2553 2937 3095 3119 3128 3297 4138
Size: 1009 1544 384 158 24 9 169 841
Cuts at: 4138 4389 4542
Size: 251 153
Fragments arranged by size:
1544 1009 841 384 251 169 158 153
24 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 748 1258 4542
Size: 748 510 3284
Fragments arranged by size:
3284 748 510
EcoICRI GAG'CTC
Cuts at: 0 1289 2198 2966 4275 4542
Size: 1289 909 768 1309 267
Fragments arranged by size:
1309 1289 909 768 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 341 1031 1197 1418 1507 1564 1600
Size: 170 171 690 166 221 89 57 36
Cuts at: 1600 1823 2408 2906 3065 3170 3227 3455 3562
Size: 223 585 498 159 105 57 228 107
Cuts at: 3562 4267 4370 4504 4542
Size: 705 103 134 38
Fragments arranged by size:
705 690 585 498 228 223 221 171
170 166 159 134 107 105 103 89
57 57 38 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3647 4219 4542
Size: 3647 572 323
Fragments arranged by size:
3647 572 323
EcoO109I rG'GnC_Cy
Cuts at: 0 99 1122 1374 2316 2548 2649 2709 3151
Size: 99 1023 252 942 232 101 60 442
Cuts at: 3151 3248 3249 3261 3639 3960 4141 4476 4542
Size: 97 1 12 378 321 181 335 66
Fragments arranged by size:
1023 942 442 378 335 321 252 232
181 101 99 97 66 60 12 1
EcoRV GAT'ATC
Cuts at: 0 3773 4542
Size: 3773 769
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 135 2413 4002 4542
Size: 135 2278 1589 540
Fragments arranged by size:
2278 1589 540 135
FspI TGC'GCA
Cuts at: 0 70 1793 4542
Size: 70 1723 2749
Fragments arranged by size:
2749 1723 70
HaeII r_GCGC'y
Cuts at: 0 437 2193 2568 4542
Size: 437 1756 375 1974
Fragments arranged by size:
1974 1756 437 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 24 56 91 123 163 195 1041 1073
Size: 24 32 35 32 40 32 846 32
Cuts at: 1073 1681 1713 2056 2088 2211 2243 2330 2362
Size: 608 32 343 32 123 32 87 32
Cuts at: 2362 3070 3102 3500 3532 3663 3695 4366 4398
Size: 708 32 398 32 131 32 671 32
Cuts at: 4398 4542
Size: 144
Fragments arranged by size:
846 708 671 608 398 343 144 131
123 87 40 35 32 32 32 32
32 32 32 32 32 32 32 32
24
HincII GTy'rAC
Cuts at: 0 17 480 4542
Size: 17 463 4062
Fragments arranged by size:
4062 463 17
KasI G'GCGC_C
Cuts at: 0 2189 4542
Size: 2189 2353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1406 4333 4542
Size: 542 864 2927 209
Fragments arranged by size:
2927 864 542 209
MscI TGG'CCA
Cuts at: 0 2089 2102 2671 3229 3553 3766 4542
Size: 2089 13 569 558 324 213 776
Fragments arranged by size:
2089 776 569 558 324 213 13
MslI CAynn'nnrTG
Cuts at: 0 401 508 706 859 1354 1867 1996 2122
Size: 401 107 198 153 495 513 129 126
Cuts at: 2122 2158 2182 2215 4542
Size: 36 24 33 2327
Fragments arranged by size:
2327 513 495 401 198 153 129 126
107 36 33 24
MspA1I CmG'CkG
Cuts at: 0 1757 1880 2036 2174 2253 2622 3017 3109
Size: 1757 123 156 138 79 369 395 92
Cuts at: 3109 3533 3709 4237 4263 4280 4542
Size: 424 176 528 26 17 262
Fragments arranged by size:
1757 528 424 395 369 262 176 156
138 123 92 79 26 17
NarI GG'CG_CC
Cuts at: 0 2190 4542
Size: 2190 2352
NcoI C'CATG_G
Cuts at: 0 2891 3761 4187 4542
Size: 2891 870 426 355
Fragments arranged by size:
2891 870 426 355
NheI G'CTAG_C
Cuts at: 0 979 1614 4542
Size: 979 635 2928
Fragments arranged by size:
2928 979 635
NsiI A_TGCA'T
Cuts at: 0 1419 4542
Size: 1419 3123
NspI r_CATG'y
Cuts at: 0 339 4542
Size: 339 4203
PasI CC'CwG_GG
Cuts at: 0 4447 4542
Size: 4447 95
PflMI CCAn_nnn'nTGG
Cuts at: 0 963 1650 2832 3712 4317 4542
Size: 963 687 1182 880 605 225
Fragments arranged by size:
1182 963 880 687 605 225
PfoI T'CCnGG_A
Cuts at: 0 265 2334 2519 3890 4542
Size: 265 2069 185 1371 652
Fragments arranged by size:
2069 1371 652 265 185
PmlI CAC'GTG
Cuts at: 0 1836 4542
Size: 1836 2706
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4523 4542
Size: 4523 19
PpuMI rG'GwC_Cy
Cuts at: 0 99 1122 2548 2649 3261 4542
Size: 99 1023 1426 101 612 1281
Fragments arranged by size:
1426 1281 1023 612 101 99
PshAI GACnn'nnGTC
Cuts at: 0 1601 4542
Size: 1601 2941
PspOMI G'GGCC_C
Cuts at: 0 3150 3248 3959 4090 4542
Size: 3150 98 711 131 452
Fragments arranged by size:
3150 711 452 131 98
PstI C_TGCA'G
Cuts at: 0 392 585 2538 2648 3638 4542
Size: 392 193 1953 110 990 904
Fragments arranged by size:
1953 990 904 392 193 110
PvuII CAG'CTG
Cuts at: 0 1757 1880 2036 2174 2622 3017 3109 3533
Size: 1757 123 156 138 448 395 92 424
Cuts at: 3533 3709 4263 4542
Size: 176 554 279
Fragments arranged by size:
1757 554 448 424 395 279 176 156
138 123 92
SacI G_AGCT'C
Cuts at: 0 1291 2200 2968 4277 4542
Size: 1291 909 768 1309 265
Fragments arranged by size:
1309 1291 909 768 265
SacII CC_GC'GG
Cuts at: 0 4281 4542
Size: 4281 261
SanDI GG'GwC_CC
Cuts at: 0 1122 4542
Size: 1122 3420
SapI GCTCTTCn'nnn_
Cuts at: 0 3095 4542
Size: 3095 1447
SbfI CC_TGCA'GG
Cuts at: 0 2648 4542
Size: 2648 1894
ScaI AGT'ACT
Cuts at: 0 310 4542
Size: 310 4232
SexAI A'CCwGG_T
Cuts at: 0 3884 4542
Size: 3884 658
SfcI C'TryA_G
Cuts at: 0 388 581 1675 2534 2644 3163 3634 4542
Size: 388 193 1094 859 110 519 471 908
Fragments arranged by size:
1094 908 859 519 471 388 193 110
SfoI GGC'GCC
Cuts at: 0 2191 4542
Size: 2191 2351
SmaI CCC'GGG
Cuts at: 0 2929 4542
Size: 2929 1613
SmlI C'TyrA_G
Cuts at: 0 2713 3351 3821 4542
Size: 2713 638 470 721
Fragments arranged by size:
2713 721 638 470
SspI AAT'ATT
Cuts at: 0 1917 4542
Size: 1917 2625
StuI AGG'CCT
Cuts at: 0 1219 4542
Size: 1219 3323
StyI C'CwwG_G
Cuts at: 0 110 119 2891 3761 3999 4063 4137 4187
Size: 110 9 2772 870 238 64 74 50
Cuts at: 4187 4542
Size: 355
Fragments arranged by size:
2772 870 355 238 110 74 64 50
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 795 1384 1925 2436 2859 4542
Size: 795 589 541 511 423 1683
Fragments arranged by size:
1683 795 589 541 511 423
TatI w'GTAC_w
Cuts at: 0 308 440 1085 1145 1667 1886 3312 4015
Size: 308 132 645 60 522 219 1426 703
Cuts at: 4015 4542
Size: 527
Fragments arranged by size:
1426 703 645 527 522 308 219 132
60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 128 160 1687 1719 1796 1828 2396 2428
Size: 128 32 1527 32 77 32 568 32
Cuts at: 2428 4542
Size: 2114
Fragments arranged by size:
2114 1527 568 128 77 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 117 2293 2436 3759 4084 4318 4542
Size: 117 2176 143 1323 325 234 224
Fragments arranged by size:
2176 1323 325 234 224 143 117
XmaI C'CCGG_G
Cuts at: 0 2927 4542
Size: 2927 1615
XmnI GAAnn'nnTTC
Cuts at: 0 2396 4542
Size: 2396 2146
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI NheI
NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SanDI SapI
SbfI ScaI SexAI SfcI SfoI SmaI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI BaeI BlpI BsaWI BsiEI
BsiWI BspEI BspHI BssHII BssSI BstBI BstZ17I BtgZI
ClaI EagI EcoRI FseI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NotI NruI PacI
PciI PmeI PsiI PspXI PsrI PvuI RsrII SalI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XhoI ZraI