Restriction map for
pFN21AA0581
(Linear) (Six-base) MAPSORT of: KIAA0581.seq Check: 1500 from: 1 to: 3648
KIAA0581.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2679 3417 3648
Size: 2679 738 231
Fragments arranged by size:
2679 738 231
AccI GT'mk_AC
Cuts at: 0 2372 3648
Size: 2372 1276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2018 3648
Size: 2018 1630
AflII C'TTAA_G
Cuts at: 0 475 751 3648
Size: 475 276 2897
Fragments arranged by size:
2897 475 276
AflIII A'CryG_T
Cuts at: 0 434 950 1804 1806 3648
Size: 434 516 854 2 1842
Fragments arranged by size:
1842 854 516 434 2
AleI CACnn'nnGTG
Cuts at: 0 313 3648
Size: 313 3335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2498 3648
Size: 2498 1150
AlwNI CAG_nnn'CTG
Cuts at: 0 2349 2659 3045 3405 3568 3648
Size: 2349 310 386 360 163 80
Fragments arranged by size:
2349 386 360 310 163 80
ApaLI G'TGCA_C
Cuts at: 0 22 3648
Size: 22 3626
ApoI r'AATT_y
Cuts at: 0 79 691 1382 1667 1753 2263 2861 3457
Size: 79 612 691 285 86 510 598 596
Cuts at: 3457 3487 3631 3648
Size: 30 144 17
Fragments arranged by size:
691 612 598 596 510 285 144 86
79 30 17
AseI AT'TA_AT
Cuts at: 0 980 3648
Size: 980 2668
AvaI C'yCGr_G
Cuts at: 0 3475 3648
Size: 3475 173
BanI G'GyrC_C
Cuts at: 0 73 287 2667 3648
Size: 73 214 2380 981
Fragments arranged by size:
2380 981 214 73
BanII G_rGCy'C
Cuts at: 0 12 1319 1496 3648
Size: 12 1307 177 2152
Fragments arranged by size:
2152 1307 177 12
BbeI G_GCGC'C
Cuts at: 0 291 3648
Size: 291 3357
BbsI GAAGACnn'nnnn_
Cuts at: 0 571 953 1694 3289 3530 3648
Size: 571 382 741 1595 241 118
Fragments arranged by size:
1595 741 571 382 241 118
BbvCI CC'TCA_GC
Cuts at: 0 1832 3648
Size: 1832 1816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 63 97 2281 2315 3648
Size: 63 34 2184 34 1333
Fragments arranged by size:
2184 1333 63 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2112 3648
Size: 2112 1536
BclI T'GATC_A
Cuts at: 0 305 726 2949 3648
Size: 305 421 2223 699
Fragments arranged by size:
2223 699 421 305
BfrBI ATG'CAT
Cuts at: 0 448 3648
Size: 448 3200
BglI GCCn_nnn'nGGC
Cuts at: 0 1270 3648
Size: 1270 2378
BglII A'GATC_T
Cuts at: 0 2685 3648
Size: 2685 963
BlpI GC'TnA_GC
Cuts at: 0 1780 2471 3648
Size: 1780 691 1177
Fragments arranged by size:
1780 1177 691
Bme1580I G_kGCm'C
Cuts at: 0 26 76 3648
Size: 26 50 3572
Fragments arranged by size:
3572 50 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 661 2588 3648
Size: 661 1927 1060
Fragments arranged by size:
1927 1060 661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 476 608 1364 1464 1730 2552 2558
Size: 313 163 132 756 100 266 822 6
Cuts at: 2558 2636 3457 3579 3648
Size: 78 821 122 69
Fragments arranged by size:
822 821 756 313 266 163 132 122
100 78 69 6
Bpu10I CC'TnA_GC
Cuts at: 0 1832 3648
Size: 1832 1816
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 49 593 2898 3648
Size: 22 27 544 2305 750
Fragments arranged by size:
2305 750 544 27 22
BsaAI yAC'GTr
Cuts at: 0 259 1807 2088 3648
Size: 259 1548 281 1560
Fragments arranged by size:
1560 1548 281 259
BsaHI Gr'CG_yC
Cuts at: 0 288 3648
Size: 288 3360
BsaWI w'CCGG_w
Cuts at: 0 3296 3648
Size: 3296 352
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1459 1489 1728 1758 3648
Size: 1459 30 239 30 1890
Fragments arranged by size:
1890 1459 239 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1020 1467 1980 3292 3616 3626 3632 3648
Size: 1020 447 513 1312 324 10 6 16
Fragments arranged by size:
1312 1020 513 447 324 16 10 6
BseYI C'CCAG_C
Cuts at: 0 2938 3606 3648
Size: 2938 668 42
Fragments arranged by size:
2938 668 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3340 3514 3648
Size: 3340 174 134
Fragments arranged by size:
3340 174 134
BsiHKAI G_wGCw'C
Cuts at: 0 26 1058 3648
Size: 26 1032 2590
Fragments arranged by size:
2590 1032 26
BsmI GAATG_Cn'
Cuts at: 0 1851 3648
Size: 1851 1797
Bsp1286I G_dGCh'C
Cuts at: 0 12 26 76 1058 1319 1496 3648
Size: 12 14 50 982 261 177 2152
Fragments arranged by size:
2152 982 261 177 50 14 12
BspHI T'CATG_A
Cuts at: 0 1961 3648
Size: 1961 1687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 428 1840 2679 3417 3648
Size: 428 1412 839 738 231
Fragments arranged by size:
1412 839 738 428 231
BsrGI T'GTAC_A
Cuts at: 0 632 3648
Size: 632 3016
BssSI C'ACGA_G
Cuts at: 0 331 355 3648
Size: 331 24 3293
Fragments arranged by size:
3293 331 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 30 3045 3648
Size: 30 3015 603
Fragments arranged by size:
3015 603 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1021 3648
Size: 1021 2627
BstYI r'GATC_y
Cuts at: 0 192 762 2685 3401 3648
Size: 192 570 1923 716 247
Fragments arranged by size:
1923 716 570 247 192
Bsu36I CC'TnA_GG
Cuts at: 0 140 3648
Size: 140 3508
BtgI C'CryG_G
Cuts at: 0 1131 3648
Size: 1131 2517
EaeI y'GGCC_r
Cuts at: 0 2 377 1011 1604 2271 2302 3648
Size: 2 375 634 593 667 31 1346
Fragments arranged by size:
1346 667 634 593 375 31 2
EarI CTCTTCn'nnn_
Cuts at: 0 1106 1358 1843 1927 3134 3269 3648
Size: 1106 252 485 84 1207 135 379
Fragments arranged by size:
1207 1106 485 379 252 135 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1288 2895 3648
Size: 1288 1607 753
Fragments arranged by size:
1607 1288 753
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 476 608 1364 1464 1730 2018 2552
Size: 313 163 132 756 100 266 288 534
Cuts at: 2552 2558 2636 3457 3579 3648
Size: 6 78 821 122 69
Fragments arranged by size:
821 756 534 313 288 266 163 132
122 100 78 69 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 3648
Size: 67 3581
EcoO109I rG'GnC_Cy
Cuts at: 0 246 3648
Size: 246 3402
EcoRI G'AATT_C
Cuts at: 0 3487 3648
Size: 3487 161
HaeII r_GCGC'y
Cuts at: 0 291 3648
Size: 291 3357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 123 155 1483 1515 3458 3490
Size: 85 32 6 32 1328 32 1943 32
Cuts at: 3490 3602 3634 3648
Size: 112 32 14
Fragments arranged by size:
1943 1328 112 85 32 32 32 32
32 14 6
HincII GTy'rAC
Cuts at: 0 3125 3648
Size: 3125 523
HindIII A'AGCT_T
Cuts at: 0 2423 3648
Size: 2423 1225
KasI G'GCGC_C
Cuts at: 0 287 3648
Size: 287 3361
MfeI C'AATT_G
Cuts at: 0 1181 2476 3648
Size: 1181 1295 1172
Fragments arranged by size:
1295 1181 1172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1484 1844 3648
Size: 80 1404 360 1804
Fragments arranged by size:
1804 1404 360 80
MscI TGG'CCA
Cuts at: 0 379 1013 1606 2273 3648
Size: 379 634 593 667 1375
Fragments arranged by size:
1375 667 634 593 379
MslI CAynn'nnrTG
Cuts at: 0 313 2380 3648
Size: 313 2067 1268
Fragments arranged by size:
2067 1268 313
MspA1I CmG'CkG
Cuts at: 0 1009 3045 3648
Size: 1009 2036 603
Fragments arranged by size:
2036 1009 603
NarI GG'CG_CC
Cuts at: 0 288 3648
Size: 288 3360
NcoI C'CATG_G
Cuts at: 0 1131 3648
Size: 1131 2517
NdeI CA'TA_TG
Cuts at: 0 2406 3648
Size: 2406 1242
NsiI A_TGCA'T
Cuts at: 0 450 3648
Size: 450 3198
NspI r_CATG'y
Cuts at: 0 438 954 1488 3648
Size: 438 516 534 2160
Fragments arranged by size:
2160 534 516 438
PciI A'CATG_T
Cuts at: 0 434 950 3648
Size: 434 516 2698
Fragments arranged by size:
2698 516 434
PflMI CCAn_nnn'nTGG
Cuts at: 0 386 1884 3315 3648
Size: 386 1498 1431 333
Fragments arranged by size:
1498 1431 386 333
PfoI T'CCnGG_A
Cuts at: 0 2529 3308 3404 3648
Size: 2529 779 96 244
Fragments arranged by size:
2529 779 244 96
PmlI CAC'GTG
Cuts at: 0 1807 3648
Size: 1807 1841
PpuMI rG'GwC_Cy
Cuts at: 0 246 3648
Size: 246 3402
PshAI GACnn'nnGTC
Cuts at: 0 3130 3263 3648
Size: 3130 133 385
Fragments arranged by size:
3130 385 133
PstI C_TGCA'G
Cuts at: 0 1110 3428 3648
Size: 1110 2318 220
Fragments arranged by size:
2318 1110 220
PvuII CAG'CTG
Cuts at: 0 1009 3045 3648
Size: 1009 2036 603
Fragments arranged by size:
2036 1009 603
SapI GCTCTTCn'nnn_
Cuts at: 0 1843 3648
Size: 1843 1805
ScaI AGT'ACT
Cuts at: 0 1279 3648
Size: 1279 2369
SexAI A'CCwGG_T
Cuts at: 0 2795 3648
Size: 2795 853
SfcI C'TryA_G
Cuts at: 0 1106 1467 1941 3424 3648
Size: 1106 361 474 1483 224
Fragments arranged by size:
1483 1106 474 361 224
SfoI GGC'GCC
Cuts at: 0 289 3648
Size: 289 3359
SmlI C'TyrA_G
Cuts at: 0 37 64 475 608 751 2877 3475 3648
Size: 37 27 411 133 143 2126 598 173
Fragments arranged by size:
2126 598 411 173 143 133 37 27
StuI AGG'CCT
Cuts at: 0 1971 3648
Size: 1971 1677
StyI C'CwwG_G
Cuts at: 0 242 380 1131 2154 3648
Size: 242 138 751 1023 1494
Fragments arranged by size:
1494 1023 751 242 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1144 2628 3648
Size: 1144 1484 1020
Fragments arranged by size:
1484 1144 1020
TatI w'GTAC_w
Cuts at: 0 632 1277 3648
Size: 632 645 2371
Fragments arranged by size:
2371 645 632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1728 1760 1793 1825 1859 1891 2150 2182
Size: 1728 32 33 32 34 32 259 32
Cuts at: 2182 3648
Size: 1466
Fragments arranged by size:
1728 1466 259 34 33 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1746 3426 3603 3648
Size: 1746 1680 177 45
Fragments arranged by size:
1746 1680 177 45
XhoI C'TCGA_G
Cuts at: 0 3475 3648
Size: 3475 173
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HincII HindIII KasI MfeI MmeI MscI MslI
MspA1I NarI NcoI NdeI NsiI NspI PciI PflMI
PfoI PmlI PpuMI PshAI PstI PvuII SapI ScaI
SexAI SfcI SfoI SmlI StuI StyI TaqII TatI
TstI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AloI ApaI
AscI AsiSI AvrII BaeI BamHI BmgBI BmrI BmtI
BsaI BsaBI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI
BsrFI BssHII BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FalI
FseI FspI FspAI HpaI KpnI MluI NaeI NgoMIV
NheI NotI NruI PacI PasI PmeI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XmaI XmnI
ZraI