Restriction map for
pFN21AA0521
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# NO CUT ENZYMES ( 81 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AscI AseI AsnI Asp718I AviII
AvrII BcgI BciVI BfrI BlnI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EcoO65I EcoRI EcoT22I
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MroI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SapI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 3031 GACGTC GACGT^C
AccI 2052 GTCGAC GT^[AC][GT]AC
AflIII 2056 ACGCGT A^C[GA][CT]GT
Alw44I 340 GTGCAC G^TGCAC
AocI 921 CCTCAGG CC^T[ACGT]AGG
ApaLI 340 GTGCAC G^TGCAC
Asp700I 2034 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1934 GGATCC G^GATCC
BanI 1872 GGCGCC G^G[CT][GA]CC
BbeI 1872 GGCGCC GGCGC^C
BbrPI 346 CACGTG CAC^GTG
BbvCI 198 GCTGAGG CCTCAGC(-5/-2)
BfaI 1456 CTAG C^TAG
BglII 1469 AGATCT A^GATCT
BsaAI 346 CACGTG [CT]AC^GT[GA]
BsaWI 527 ACCGGA [AT]^CCGG[AT]
BsiEI 2648 CGGCCG CG[GA][CT]^CG
BsmI 746 GCATTC GAATGC(1/-1)
Bsu36I 921 CCTCAGG CC^T[ACGT]AGG
DraI 108 TTTAAA TTT^AAA
DraIII 343 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 886 GACGCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2648 CGGCCG C^GGCCG
EclXI 2648 CGGCCG C^GGCCG
Eco52I 2648 CGGCCG C^GGCCG
Eco81I 921 CCTCAGG CC^T[ACGT]AGG
HincII 2052 GTCGAC GT[CT]^[GA]AC
HindII 2052 GTCGAC GT[CT]^[GA]AC
KasI 1872 GGCGCC G^GCGCC
MaeI 1456 CTAG C^TAG
MluI 2056 ACGCGT A^CGCGT
NarI 1872 GGCGCC GG^CGCC
PmaCI 346 CACGTG CAC^GTG
PmlI 346 CACGTG CAC^GTG
SalI 2052 GTCGAC G^TCGAC
SfoI 1872 GGCGCC GGC^GCC
XmnI 2034 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcsI 112 AAATTT [GA]^AATT[CT]
679 AAATTT
Aor51HI 2165 AGCGCT AGC^GCT
2732 AGCGCT
ApoI 112 AAATTT [GA]^AATT[CT]
679 AAATTT
AspI 1941 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2852 GACACAGTC
BclI 692 TGATCA T^GATCA
1220 TGATCA
BglI 41 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1288 GCCTGGATGGC
BsmBI 460 GAGACG CGTCTC(1/5)
2002 GAGACG
BsrFI 2582 GCCGGC [GA]^CCGG[CT]
2988 GCCGGC
BstXI 1904 CCACCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2669 CCACCAAGCTGG
Cfr10I 2582 GCCGGC [GA]^CCGG[CT]
2988 GCCGGC
EarI 433 CTCTTC CTCTTC(1/4)
1360 GAAGAG
Eco47III 2165 AGCGCT AGC^GCT
2732 AGCGCT
EcoRV 868 GATATC GAT^ATC
1066 GATATC
FbaI 692 TGATCA T^GATCA
1220 TGATCA
Ksp632I 433 CTCTTC CTCTTC(1/4)
1360 GAAGAG
MseI 109 TTAA T^TAA
116 TTAA
NaeI 2582 GCCGGC GCC^GGC
2988 GCCGGC
NgoMIV 2582 GCCGGC G^CCGGC
2988 GCCGGC
PflFI 1941 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2852 GACACAGTC
PflMI 2196 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2872 CCAGACCCTGG
PpuMI 21 GGGTCCC [GA]G^G[AT]CC[CT]
1541 GGGACCC
SbfI 390 CCTGCAGG CCTGCA^GG
1557 CCTGCAGG
SphI 1028 GCATGC GCATG^C
2504 GCATGC
Sse8387I 390 CCTGCAGG CCTGCA^GG
1557 CCTGCAGG
Tru9I 109 TTAA T^TAA
116 TTAA
Tth111I 1941 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2852 GACACAGTC
Van91I 2196 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2872 CCAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI ApaI AspEI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII