(Circular) (Six-base) MAPSORT of: pFN21AA0521.seq Check: 4127 from: 1 to: 7750 pFN21AA0521 7750 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3856 3856 Size: 7750 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5040 5560 278 Size: 53 83 186 936 3504 520 2468 Fragments arranged by size: 3504 2468 936 520 186 83 53 AccI GT'mk_AC Cuts at: 1937 4058 5093 1937 Size: 2121 1035 4594 Fragments arranged by size: 4594 2121 1035 Acc65I G'GTAC_C Cuts at: 5071 5071 Size: 7750 AclI AA'CG_TT Cuts at: 1563 5877 6250 1563 Size: 4314 373 3063 Fragments arranged by size: 4314 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2396 2654 2975 2993 3337 3956 4787 5813 Size: 764 258 321 18 344 619 831 1026 Cuts at: 5813 7197 7465 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1026 831 764 619 344 321 268 258 18 AfeI AGC'GCT Cuts at: 4172 4739 4172 Size: 567 7183 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7553 AflIII A'CryG_T Cuts at: 1203 4061 6649 7626 1203 Size: 2858 2588 977 1327 Fragments arranged by size: 2858 2588 1327 977 AgeI A'CCGG_T Cuts at: 6581 6581 Size: 7750 AhdI GACnn_n'nnGTC Cuts at: 2143 2315 2956 6479 2143 Size: 172 641 3523 3414 Fragments arranged by size: 3523 3414 641 172 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7750 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4035 1184 Size: 2851 4899 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2396 2428 4028 4060 2396 Size: 32 1600 32 6086 Fragments arranged by size: 6086 1600 32 32 AlwNI CAG_nnn'CTG Cuts at: 3619 3949 4207 4777 4780 4883 5493 7065 3619 Size: 330 258 570 3 103 610 1572 4304 Fragments arranged by size: 4304 1572 610 570 330 258 103 3 ApaI G_GGCC'C Cuts at: 2395 3351 4499 2395 Size: 956 1148 5646 Fragments arranged by size: 5646 1148 956 ApaLI G'TGCA_C Cuts at: 2345 5807 6963 2345 Size: 3462 1156 3132 Fragments arranged by size: 3462 3132 1156 ApoI r'AATT_y Cuts at: 1493 2117 2684 5059 5285 5437 7546 1493 Size: 624 567 2375 226 152 2109 1697 Fragments arranged by size: 2375 2109 1697 624 567 226 152 AseI AT'TA_AT Cuts at: 160 1032 6304 160 Size: 872 5272 1606 Fragments arranged by size: 5272 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7750 AvaI C'yCGr_G Cuts at: 1870 1943 3525 3783 4500 4550 4897 5075 1870 Size: 73 1582 258 717 50 347 178 4545 Fragments arranged by size: 4545 1582 717 347 258 178 73 50 BamHI G'GATC_C Cuts at: 3939 5080 5447 3939 Size: 1141 367 6242 Fragments arranged by size: 6242 1141 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3877 5071 6526 618 Size: 325 217 627 2090 1194 1455 1842 Fragments arranged by size: 2090 1842 1455 1194 627 325 217 BanII G_rGCy'C Cuts at: 1391 2048 2395 2985 3275 3351 3851 4499 4733 Size: 657 347 590 290 76 500 648 234 Cuts at: 4733 4751 4841 4904 5069 1391 Size: 18 90 63 165 4072 Fragments arranged by size: 4072 657 648 590 500 347 290 234 165 90 76 63 18 BbeI G_GCGC'C Cuts at: 3881 3881 Size: 7750 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2847 2952 3006 3053 3516 3666 5040 Size: 971 948 105 54 47 463 150 1374 Cuts at: 5040 928 Size: 3638 Fragments arranged by size: 3638 1374 971 948 463 150 105 54 47 BbvCI CC'TCA_GC Cuts at: 2204 2204 Size: 7750 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5941 5975 1903 Size: 34 4004 34 3678 Fragments arranged by size: 4004 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5642 6852 5642 Size: 1210 6540 BclI T'GATC_A Cuts at: 1548 2697 3225 1548 Size: 1149 528 6073 Fragments arranged by size: 6073 1149 528 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 3299 6361 Size: 107 122 71 631 605 380 1247 3062 Cuts at: 6361 136 Size: 1525 Fragments arranged by size: 3062 1525 1247 631 605 380 122 107 71 BglII A'GATC_T Cuts at: 3474 7745 3474 Size: 4271 3479 BlpI GC'TnA_GC Cuts at: 4622 4750 4835 5167 4622 Size: 128 85 332 7205 Fragments arranged by size: 7205 332 128 85 Bme1580I G_kGCm'C Cuts at: 1790 2349 2395 3351 4499 4611 4955 5811 6967 Size: 559 46 956 1148 112 344 856 1156 Cuts at: 6967 1790 Size: 2573 Fragments arranged by size: 2573 1156 1148 956 856 559 344 112 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7750 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2780 5481 6439 Size: 416 53 83 399 24 1363 2701 958 Cuts at: 6439 442 Size: 1753 Fragments arranged by size: 2701 1753 1363 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7750 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4831 4951 4831 Size: 120 7630 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2272 2371 2483 2537 3047 3503 3938 4100 Size: 1033 99 112 54 510 456 435 162 Cuts at: 4100 4154 4229 4292 4313 4346 4397 4511 6410 Size: 54 75 63 21 33 51 114 1899 Cuts at: 6410 1239 Size: 2579 Fragments arranged by size: 2579 1899 1033 510 456 435 162 114 112 99 75 63 54 54 51 33 21 Bpu10I CC'TnA_GC Cuts at: 2204 2204 Size: 7750 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2348 2477 2957 3566 4379 4925 5875 6740 7038 Size: 129 480 609 813 546 950 865 298 Cuts at: 7038 2348 Size: 3060 Fragments arranged by size: 3060 950 865 813 609 546 480 298 129 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2017 3551 3692 6413 882 Size: 617 518 1534 141 2721 2219 Fragments arranged by size: 2721 2219 1534 617 518 141 BsaAI yAC'GTr Cuts at: 493 1204 2353 5541 493 Size: 711 1149 3188 2702 Fragments arranged by size: 3188 2702 1149 711 BsaBI GATnn'nnATC Cuts at: 7744 7744 Size: 7750 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2892 3878 5022 5037 Size: 53 83 186 936 1359 986 1144 15 Cuts at: 5037 5557 5939 6574 275 Size: 520 382 635 1451 Fragments arranged by size: 1451 1359 1144 986 936 635 520 382 186 83 53 15 BsaWI w'CCGG_w Cuts at: 2532 6183 6581 6855 7002 7413 2532 Size: 3651 398 274 147 411 2869 Fragments arranged by size: 3651 2869 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 3257 3287 3441 Size: 30 165 30 993 30 831 30 154 Cuts at: 3441 3471 4028 4058 4907 4937 1178 Size: 30 557 30 849 30 3991 Fragments arranged by size: 3991 993 849 831 557 165 154 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2221 2417 2534 3221 3353 3356 4133 1186 Size: 1035 196 117 687 132 3 777 4803 Fragments arranged by size: 4803 1035 777 687 196 132 117 3 BseYI C'CCAG_C Cuts at: 2031 3519 3634 3954 4173 4344 4449 4469 4740 Size: 1488 115 320 219 171 105 20 271 Cuts at: 4740 4975 6953 7449 2031 Size: 235 1978 496 2332 Fragments arranged by size: 2332 1978 1488 496 320 271 235 219 171 115 105 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2762 3703 4679 1163 Size: 573 1026 941 976 4234 Fragments arranged by size: 4234 1026 976 941 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4656 5200 5961 6989 1540 Size: 275 186 2655 544 761 1028 2301 Fragments arranged by size: 2655 2301 1028 761 544 275 186 BsiHKAI G_wGCw'C Cuts at: 2349 3275 3851 4751 4814 5811 5896 6967 2349 Size: 926 576 900 63 997 85 1071 3132 Fragments arranged by size: 3132 1071 997 926 900 576 85 63 BsmI GAATG_Cn' Cuts at: 2751 5261 5354 2751 Size: 2510 93 5147 Fragments arranged by size: 5147 2510 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2459 4001 2459 Size: 1542 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2349 2395 2985 3275 3351 3851 Size: 399 258 301 46 590 290 76 500 Cuts at: 3851 4499 4611 4733 4751 4814 4841 4904 4955 Size: 648 112 122 18 63 27 63 51 Cuts at: 4955 5069 5811 5896 6967 1391 Size: 114 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 648 590 500 399 301 290 258 122 114 112 85 76 63 63 51 46 27 18 BspEI T'CCGG_A Cuts at: 7413 7413 Size: 7750 BspHI T'CATG_A Cuts at: 5639 7369 5639 Size: 1730 6020 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2466 3522 3856 4080 5105 844 Size: 1622 1056 334 224 1025 3489 Fragments arranged by size: 3489 1622 1056 1025 334 224 BsrBI CCG'CTC Cuts at: 1394 1823 3421 4033 4090 4267 4282 4330 4366 Size: 429 1598 612 57 177 15 48 36 Cuts at: 4366 4830 5637 1394 Size: 464 807 3507 Fragments arranged by size: 3507 1598 807 612 464 429 177 57 48 36 15 BsrDI GCAATG_nn' Cuts at: 65 1248 6245 6419 65 Size: 1183 4997 174 1396 Fragments arranged by size: 4997 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4587 4993 6394 6581 1700 Size: 110 2777 406 1401 187 2869 Fragments arranged by size: 2869 2777 1401 406 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7750 BssHII G'CGCG_C Cuts at: 1925 4123 4221 4419 1925 Size: 2198 98 198 5256 Fragments arranged by size: 5256 2198 198 98 BssSI C'ACGA_G Cuts at: 2231 3268 3397 4160 5810 6822 2231 Size: 1037 129 763 1650 1012 3159 Fragments arranged by size: 3159 1650 1037 1012 763 129 BstAPI GCAn_nnn'nTGC Cuts at: 794 2353 3039 4777 4780 794 Size: 1559 686 1738 3 3764 Fragments arranged by size: 3764 1738 1559 686 3 BstBI TT'CG_AA Cuts at: 6554 6554 Size: 7750 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7750 BstXI CCAn_nnnn'nTGG Cuts at: 3916 4681 3916 Size: 765 6985 BstYI r'GATC_y Cuts at: 1972 2185 3474 3939 4668 5080 5447 5832 5849 Size: 213 1289 465 729 412 367 385 17 Cuts at: 5849 7301 7387 7399 7745 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1289 729 465 412 385 367 346 213 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2927 2927 Size: 7750 BtgI C'CryG_G Cuts at: 513 1065 2262 3538 4353 513 Size: 552 1197 1276 815 3910 Fragments arranged by size: 3910 1276 1197 815 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3230 4617 487 Size: 683 56 2004 1387 3620 Fragments arranged by size: 3620 2004 1387 683 56 BtsI GCAGTG_nn' Cuts at: 2533 4988 5266 6060 6080 2533 Size: 2455 278 794 20 4203 Fragments arranged by size: 4203 2455 794 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7750 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7715 DraI TTT'AAA Cuts at: 2115 5054 2115 Size: 2939 4811 DraIII CAC_nnn'GTG Cuts at: 2353 2353 Size: 7750 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2897 6757 809 Size: 507 1581 3860 1802 Fragments arranged by size: 3860 1802 1581 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2227 2431 2632 Size: 54 1425 50 275 18 397 204 201 Cuts at: 2632 3198 3489 3588 3649 4440 4593 4653 4995 Size: 566 291 99 61 791 153 60 342 Cuts at: 4995 5197 6086 7715 8 Size: 202 889 1629 43 Fragments arranged by size: 1629 1425 889 791 566 397 342 291 275 204 202 201 153 99 61 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4653 5197 1537 Size: 275 2841 544 4090 Fragments arranged by size: 4090 2841 544 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2444 3360 5680 1353 Size: 1091 916 2320 3423 Fragments arranged by size: 3423 2320 1091 916 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3671 6337 6711 6857 387 Size: 1064 138 2082 2666 374 146 1280 Fragments arranged by size: 2666 2082 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3273 3849 4749 3273 Size: 576 900 6274 Fragments arranged by size: 6274 900 576 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 2371 2396 2483 2537 2654 2975 Size: 393 640 99 25 87 54 117 321 Cuts at: 2975 2993 3047 3337 3503 3938 3956 4100 4154 Size: 18 54 290 166 435 18 144 54 Cuts at: 4154 4229 4292 4313 4346 4397 4511 4787 5813 Size: 75 63 21 33 51 114 276 1026 Cuts at: 5813 6410 7197 7465 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1026 787 640 597 435 393 321 290 276 268 166 144 117 114 99 87 75 63 54 54 54 51 33 25 21 18 18 EcoNI CCTnn'n_nnAGG Cuts at: 2203 2399 2519 2849 2203 Size: 196 120 330 7104 Fragments arranged by size: 7104 330 196 120 EcoO109I rG'GnC_Cy Cuts at: 2027 2391 2392 3347 3348 3547 3780 3797 4495 Size: 364 1 955 1 199 233 17 698 Cuts at: 4495 4496 2027 Size: 1 5281 Fragments arranged by size: 5281 955 698 364 233 199 17 1 1 1 EcoRI G'AATT_C Cuts at: 5059 5437 5059 Size: 378 7372 EcoRV GAT'ATC Cuts at: 2875 3073 2875 Size: 198 7552 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2336 3023 2336 Size: 687 7063 FspI TGC'GCA Cuts at: 6256 6256 Size: 7750 HaeII r_GCGC'y Cuts at: 1678 2503 2926 3064 3234 3645 3881 4174 4189 Size: 825 423 138 170 411 236 293 15 Cuts at: 4189 4300 4306 4375 4549 4575 4629 4741 5013 Size: 111 6 69 174 26 54 112 272 Cuts at: 5013 6897 1678 Size: 1884 2531 Fragments arranged by size: 2531 1884 825 423 411 293 272 236 174 170 138 112 111 69 54 26 15 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2129 2161 2204 Size: 32 135 32 427 32 98 32 43 Cuts at: 2204 2236 2301 2333 2864 2896 2942 2974 4907 Size: 32 65 32 531 32 46 32 1933 Cuts at: 4907 4939 6391 6423 6465 6497 1373 Size: 32 1452 32 42 32 2626 Fragments arranged by size: 2626 1933 1452 531 427 135 98 65 46 43 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4059 5094 5340 669 Size: 988 281 2121 1035 246 3079 Fragments arranged by size: 3079 2121 1035 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7750 HpaI GTT'AAC Cuts at: 5340 5340 Size: 7750 KasI G'GCGC_C Cuts at: 3877 3877 Size: 7750 KpnI G_GTAC'C Cuts at: 5075 5075 Size: 7750 MfeI C'AATT_G Cuts at: 5349 5349 Size: 7750 MluI A'CGCG_T Cuts at: 4061 7626 4061 Size: 3565 4185 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3903 6864 7048 1305 Size: 2598 2961 184 2007 Fragments arranged by size: 2961 2598 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2634 3200 4595 10 Size: 54 1425 343 802 566 1395 3165 Fragments arranged by size: 3165 1425 1395 802 566 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5708 6067 6226 518 Size: 648 4542 359 159 2042 Fragments arranged by size: 4542 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2132 3013 3442 3937 3958 4015 4114 4153 Size: 526 881 429 495 21 57 99 39 Cuts at: 4153 4618 4777 4913 5166 5843 6991 7236 1606 Size: 465 159 136 253 677 1148 245 2120 Fragments arranged by size: 2120 1148 881 677 526 495 465 429 253 245 159 136 99 57 39 21 NaeI GCC'GGC Cuts at: 4589 4995 4589 Size: 406 7344 NarI GG'CG_CC Cuts at: 3878 3878 Size: 7750 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7198 NdeI CA'TA_TG Cuts at: 387 387 Size: 7750 NgoMIV G'CCGG_C Cuts at: 4587 4993 4587 Size: 406 7344 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7750 NotI GC'GGCC_GC Cuts at: 5197 5197 Size: 7750 NspI r_CATG'y Cuts at: 1132 1229 3007 3037 4513 5108 6653 1132 Size: 97 1778 30 1476 595 1545 2229 Fragments arranged by size: 2229 1778 1545 1476 595 97 30 PasI CC'CwG_GG Cuts at: 1348 2057 1348 Size: 709 7041 PciI A'CATG_T Cuts at: 6649 6649 Size: 7750 PflMI CCAn_nnn'nTGG Cuts at: 1108 4207 4883 1108 Size: 3099 676 3975 Fragments arranged by size: 3975 3099 676 PfoI T'CCnGG_A Cuts at: 2249 3405 3915 4026 2249 Size: 1156 510 111 5973 Fragments arranged by size: 5973 1156 510 111 PmeI GTTT'AAAC Cuts at: 5054 5054 Size: 7750 PmlI CAC'GTG Cuts at: 2353 2353 Size: 7750 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2396 2428 4028 4060 4814 4846 5819 5851 7332 Size: 32 1600 32 754 32 973 32 1481 Cuts at: 7332 7364 2396 Size: 32 2782 Fragments arranged by size: 2782 1600 1481 973 754 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2027 3547 2027 Size: 1520 6230 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7750 PsiI TTA'TAA Cuts at: 5320 5320 Size: 7750 PspOMI G'GGCC_C Cuts at: 2391 3347 4495 2391 Size: 956 1148 5646 Fragments arranged by size: 5646 1148 956 PstI C_TGCA'G Cuts at: 830 2400 2412 3567 3629 4056 4146 4233 5102 Size: 1570 12 1155 62 427 90 87 869 Cuts at: 5102 830 Size: 3478 Fragments arranged by size: 3478 1570 1155 869 427 90 87 62 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7750 PvuII CAG'CTG Cuts at: 2132 3442 3958 4153 4618 4777 4913 2132 Size: 1310 516 195 465 159 136 4969 Fragments arranged by size: 4969 1310 516 465 195 159 136 SacI G_AGCT'C Cuts at: 3275 3851 4751 3275 Size: 576 900 6274 Fragments arranged by size: 6274 900 576 SalI G'TCGA_C Cuts at: 1936 4057 5092 1936 Size: 2121 1035 4594 Fragments arranged by size: 4594 2121 1035 SanDI GG'GwC_CC Cuts at: 2027 3547 2027 Size: 1520 6230 SbfI CC_TGCA'GG Cuts at: 2400 3567 5102 2400 Size: 1167 1535 5048 Fragments arranged by size: 5048 1535 1167 ScaI AGT'ACT Cuts at: 5998 7710 5998 Size: 1712 6038 SfcI C'TryA_G Cuts at: 826 1045 2396 2408 3563 3625 4052 4142 4229 Size: 219 1351 12 1155 62 427 90 87 Cuts at: 4229 5098 6233 6914 7105 826 Size: 869 1135 681 191 1471 Fragments arranged by size: 1471 1351 1155 1135 869 681 427 219 191 90 87 62 12 SfoI GGC'GCC Cuts at: 3879 3879 Size: 7750 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7750 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7750 SmaI CCC'GGG Cuts at: 1872 5077 1872 Size: 3205 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2363 2492 2972 3581 4394 4940 Size: 197 926 420 129 480 609 813 546 Cuts at: 4940 5854 6755 7017 820 Size: 914 901 262 1553 Fragments arranged by size: 1553 926 914 901 813 609 546 480 420 262 197 129 SnaBI TAC'GTA Cuts at: 493 5541 493 Size: 5048 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7750 SphI G_CATG'C Cuts at: 1132 3037 4513 5108 1132 Size: 1905 1476 595 3774 Fragments arranged by size: 3774 1905 1476 595 SspI AAT'ATT Cuts at: 5 52 5674 5 Size: 47 5622 2081 Fragments arranged by size: 5622 2081 47 StuI AGG'CCT Cuts at: 1517 2855 3531 4310 1517 Size: 1338 676 779 4957 Fragments arranged by size: 4957 1338 779 676 StyI C'CwwG_G Cuts at: 513 1065 2895 4695 5013 5189 513 Size: 552 1830 1800 318 176 3074 Fragments arranged by size: 3074 1830 1800 552 318 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3107 5777 5794 5947 6132 6577 325 Size: 1255 1527 2670 17 153 185 445 1498 Fragments arranged by size: 2670 1527 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2382 5996 7708 Size: 274 80 33 51 1443 405 3614 1712 Cuts at: 7708 96 Size: 138 Fragments arranged by size: 3614 1712 1443 405 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3441 3473 7710 7742 3441 Size: 32 4237 32 3449 Fragments arranged by size: 4237 3449 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3949 4860 1316 Size: 2633 911 4206 Fragments arranged by size: 4206 2633 911 XbaI T'CTAG_A Cuts at: 5086 5086 Size: 7750 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7750 XmaI C'CCGG_G Cuts at: 1870 5075 1870 Size: 3205 4545 XmnI GAAnn'nnTTC Cuts at: 4043 5879 4043 Size: 1836 5914 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5038 5558 276 Size: 53 83 186 936 3504 520 2468 Fragments arranged by size: 3504 2468 936 520 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI