(Circular) (Six-base) MAPSORT of: pFN21AA0461.seq Check: 9003 from: 1 to: 8776 pFN21AA0461 8776 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2734 4193 2734 Size: 1459 7317 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6586 278 Size: 53 83 186 936 5050 2468 Fragments arranged by size: 5050 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6119 1937 Size: 4182 4594 Acc65I G'GTAC_C Cuts at: 2420 6097 2420 Size: 3677 5099 AclI AA'CG_TT Cuts at: 1563 6903 7276 1563 Size: 5340 373 3063 Fragments arranged by size: 5340 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3027 3464 3740 4557 5723 5936 5991 6839 Size: 1395 437 276 817 1166 213 55 848 Cuts at: 6839 8223 8491 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1395 1384 1166 848 817 437 276 268 213 55 AfeI AGC'GCT Cuts at: 5807 5807 Size: 8776 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8579 AflIII A'CryG_T Cuts at: 1203 3024 7675 8652 1203 Size: 1821 4651 977 1327 Fragments arranged by size: 4651 1821 1327 977 AgeI A'CCGG_T Cuts at: 7607 7607 Size: 8776 AhdI GACnn_n'nnGTC Cuts at: 7505 7505 Size: 8776 AleI CACnn'nnGTG Cuts at: 1166 3905 1166 Size: 2739 6037 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4397 5534 1184 Size: 3213 1137 4426 Fragments arranged by size: 4426 3213 1137 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3078 3110 4979 5011 3078 Size: 32 1869 32 6843 Fragments arranged by size: 6843 1869 32 32 AlwNI CAG_nnn'CTG Cuts at: 3137 5714 6519 8091 3137 Size: 2577 805 1572 3822 Fragments arranged by size: 3822 2577 1572 805 ApaLI G'TGCA_C Cuts at: 6833 7989 6833 Size: 1156 7620 ApoI r'AATT_y Cuts at: 1493 3301 4267 5625 6085 6311 6463 8572 1493 Size: 1808 966 1358 460 226 152 2109 1697 Fragments arranged by size: 2109 1808 1697 1358 966 460 226 152 AseI AT'TA_AT Cuts at: 160 1032 7330 160 Size: 872 6298 1606 Fragments arranged by size: 6298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8776 AvaI C'yCGr_G Cuts at: 1870 1943 3924 4194 5475 5697 6101 1870 Size: 73 1981 270 1281 222 404 4545 Fragments arranged by size: 4545 1981 1281 404 270 222 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3177 3210 5235 5268 3177 Size: 33 2025 33 6685 Fragments arranged by size: 6685 2025 33 33 BamHI G'GATC_C Cuts at: 6106 6473 6106 Size: 367 8409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2420 3765 3899 5079 6097 Size: 325 217 627 633 1345 134 1180 1018 Cuts at: 6097 7552 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1345 1180 1018 633 627 325 217 134 BanII G_rGCy'C Cuts at: 1391 4244 4694 6095 1391 Size: 2853 450 1401 4072 Fragments arranged by size: 4072 2853 1401 450 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2904 3718 4018 5934 5987 928 Size: 971 1005 814 300 1916 53 3717 Fragments arranged by size: 3717 1916 1005 971 814 300 53 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6967 7001 1903 Size: 34 5030 34 3678 Fragments arranged by size: 5030 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3580 3682 3961 5236 6668 7878 3580 Size: 102 279 1275 1432 1210 4478 Fragments arranged by size: 4478 1432 1275 1210 279 102 BclI T'GATC_A Cuts at: 1548 4215 4675 1548 Size: 2667 460 5649 Fragments arranged by size: 5649 2667 460 BfrBI ATG'CAT Cuts at: 3743 5646 3743 Size: 1903 6873 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2506 5336 5844 Size: 107 122 71 631 605 834 2830 508 Cuts at: 5844 7387 136 Size: 1543 1525 Fragments arranged by size: 2830 1543 1525 834 631 605 508 122 107 71 BglII A'GATC_T Cuts at: 3534 5043 5226 6061 8771 3534 Size: 1509 183 835 2710 3539 Fragments arranged by size: 3539 2710 1509 835 183 BlpI GC'TnA_GC Cuts at: 4530 5255 6193 4530 Size: 725 938 7113 Fragments arranged by size: 7113 938 725 Bme1580I G_kGCm'C Cuts at: 1790 2201 5300 6837 7993 1790 Size: 411 3099 1537 1156 2573 Fragments arranged by size: 3099 2573 1537 1156 411 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2419 2572 2654 Size: 416 53 83 399 24 1002 153 82 Cuts at: 2654 2933 3084 4688 6507 7465 442 Size: 279 151 1604 1819 958 1753 Fragments arranged by size: 1819 1753 1604 1002 958 416 399 279 153 151 83 82 53 24 BmtI G_CTAG'C Cuts at: 1056 2705 4718 1056 Size: 1649 2013 5114 Fragments arranged by size: 5114 2013 1649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2652 2936 3557 3621 3902 4127 7436 1239 Size: 1413 284 621 64 281 225 3309 2579 Fragments arranged by size: 3309 2579 1413 621 284 281 225 64 Bpu10I CC'TnA_GC Cuts at: 4555 4710 4555 Size: 155 8621 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4396 6901 7766 8064 4396 Size: 2505 865 298 5108 Fragments arranged by size: 5108 2505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4982 6017 7439 882 Size: 617 3483 1035 1422 2219 Fragments arranged by size: 3483 2219 1422 1035 617 BsaAI yAC'GTr Cuts at: 493 1204 3394 4156 6567 493 Size: 711 2190 762 2411 2702 Fragments arranged by size: 2702 2411 2190 762 711 BsaBI GATnn'nnATC Cuts at: 8770 8770 Size: 8776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6583 6965 7600 275 Size: 53 83 186 936 5050 382 635 1451 Fragments arranged by size: 5050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4446 7209 7607 7881 8028 8439 4446 Size: 2763 398 274 147 411 4783 Fragments arranged by size: 4783 2763 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2030 2060 3555 3585 4748 Size: 30 165 30 627 30 1495 30 1163 Cuts at: 4748 4778 4979 5009 5453 5483 1178 Size: 30 201 30 444 30 4471 Fragments arranged by size: 4471 1495 1163 627 444 201 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2054 2057 3631 3994 4461 5000 5900 Size: 865 3 3 1574 363 467 539 900 Cuts at: 5900 5921 1186 Size: 21 4041 Fragments arranged by size: 4041 1574 900 865 539 467 363 21 3 3 BseYI C'CCAG_C Cuts at: 2583 2603 3291 3971 4525 4561 4842 4956 5605 Size: 20 688 680 554 36 281 114 649 Cuts at: 5605 5758 7979 8475 2583 Size: 153 2221 496 2884 Fragments arranged by size: 2884 2221 688 680 649 554 496 281 153 114 36 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4132 4279 1163 Size: 573 2396 147 5660 Fragments arranged by size: 5660 2396 573 147 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6226 6987 8015 1540 Size: 275 186 4225 761 1028 2301 Fragments arranged by size: 4225 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6837 6922 7993 6837 Size: 85 1071 7620 Fragments arranged by size: 7620 1071 85 BsmI GAATG_Cn' Cuts at: 2251 3743 5149 5284 6287 6380 2251 Size: 1492 1406 135 1003 93 4647 Fragments arranged by size: 4647 1492 1406 1003 135 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 4244 4694 5300 6095 6837 6922 Size: 399 411 2043 450 606 795 742 85 Cuts at: 6922 7993 1391 Size: 1071 2174 Fragments arranged by size: 2174 2043 1071 795 742 606 450 411 399 85 BspEI T'CCGG_A Cuts at: 8439 8439 Size: 8776 BspHI T'CATG_A Cuts at: 3372 6665 8395 3372 Size: 3293 1730 3753 Fragments arranged by size: 3753 3293 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2734 4193 6131 844 Size: 1890 1459 1938 3489 Fragments arranged by size: 3489 1938 1890 1459 BsrBI CCG'CTC Cuts at: 1394 1823 6663 1394 Size: 429 4840 3507 Fragments arranged by size: 4840 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2349 2736 2794 3745 7271 7445 65 Size: 1183 1101 387 58 951 3526 174 1396 Fragments arranged by size: 3526 1396 1183 1101 951 387 174 58 BsrFI r'CCGG_y Cuts at: 1700 1810 3801 4092 7420 7607 1700 Size: 110 1991 291 3328 187 2869 Fragments arranged by size: 3328 2869 1991 291 187 110 BsrGI T'GTAC_A Cuts at: 96 2332 5167 96 Size: 2236 2835 3705 Fragments arranged by size: 3705 2835 2236 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8776 BssSI C'ACGA_G Cuts at: 4428 6836 7848 4428 Size: 2408 1012 5356 Fragments arranged by size: 5356 2408 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3798 5144 5714 794 Size: 3004 1346 570 3856 Fragments arranged by size: 3856 3004 1346 570 BstBI TT'CG_AA Cuts at: 2557 7580 2557 Size: 5023 3753 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8776 BstXI CCAn_nnnn'nTGG Cuts at: 2153 2846 2960 3880 4252 4345 2153 Size: 693 114 920 372 93 6584 Fragments arranged by size: 6584 920 693 372 114 93 BstYI r'GATC_y Cuts at: 1972 3534 4122 4367 5043 5226 6061 6106 6473 Size: 1562 588 245 676 183 835 45 367 Cuts at: 6473 6858 6875 8327 8413 8425 8771 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1562 1452 835 676 588 385 367 346 245 183 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 3309 5127 3309 Size: 1818 6958 BtgI C'CryG_G Cuts at: 513 1065 2004 2054 4232 4385 513 Size: 552 939 50 2178 153 4904 Fragments arranged by size: 4904 2178 939 552 153 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3234 4341 5522 487 Size: 683 56 2008 1107 1181 3741 Fragments arranged by size: 3741 2008 1181 1107 683 56 BtsI GCAGTG_nn' Cuts at: 2622 2736 3787 4159 5266 5604 6292 7086 7106 Size: 114 1051 372 1107 338 688 794 20 Cuts at: 7106 2622 Size: 4292 Fragments arranged by size: 4292 1107 1051 794 688 372 338 114 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8741 DraI TTT'AAA Cuts at: 4264 6080 4264 Size: 1816 6960 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7783 809 Size: 507 6467 1802 Fragments arranged by size: 6467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2541 2796 4335 Size: 54 1425 50 275 18 711 255 1539 Cuts at: 4335 4420 6223 7112 8741 8 Size: 85 1803 889 1629 43 Fragments arranged by size: 1803 1629 1539 1425 889 711 275 255 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6223 1537 Size: 275 4411 4090 Fragments arranged by size: 4411 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2935 3160 3767 4055 4232 4533 4923 5159 Size: 1582 225 607 288 177 301 390 236 Cuts at: 5159 5550 5967 6706 1353 Size: 391 417 739 3423 Fragments arranged by size: 3423 1582 739 607 417 391 390 301 288 236 225 177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 7363 7737 7883 387 Size: 1064 138 435 5339 374 146 1280 Fragments arranged by size: 5339 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2652 2936 3027 3464 3557 3621 3740 Size: 393 1020 284 91 437 93 64 119 Cuts at: 3740 3902 4127 4557 5723 5936 5991 6839 7436 Size: 162 225 430 1166 213 55 848 597 Cuts at: 7436 8223 8491 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1166 1020 848 787 597 437 430 393 284 268 225 213 162 119 93 91 64 55 EcoNI CCTnn'n_nnAGG Cuts at: 5126 5126 Size: 8776 EcoRI G'AATT_C Cuts at: 6085 6463 6085 Size: 378 8398 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5537 5537 Size: 8776 FspI TGC'GCA Cuts at: 7282 7282 Size: 8776 HaeII r_GCGC'y Cuts at: 1678 3978 5809 7923 1678 Size: 2300 1831 2114 2531 Fragments arranged by size: 2531 2300 2114 1831 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2164 2180 2196 2677 Size: 32 135 32 576 16 16 16 481 Cuts at: 2677 2709 4207 4239 4703 4735 4748 4780 5213 Size: 32 1498 32 464 32 13 32 433 Cuts at: 5213 5245 5453 5485 5518 5550 6014 6046 7417 Size: 32 208 32 33 32 464 32 1371 Cuts at: 7417 7449 7491 7523 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1498 1371 576 481 464 464 433 208 135 42 33 32 32 32 32 32 32 32 32 32 32 32 32 16 16 16 13 HincII GTy'rAC Cuts at: 669 1657 1938 5472 6120 6366 669 Size: 988 281 3534 648 246 3079 Fragments arranged by size: 3534 3079 988 648 281 246 HindIII A'AGCT_T Cuts at: 748 3009 3842 4775 748 Size: 2261 833 933 4749 Fragments arranged by size: 4749 2261 933 833 HpaI GTT'AAC Cuts at: 6366 6366 Size: 8776 KpnI G_GTAC'C Cuts at: 2424 6101 2424 Size: 3677 5099 MfeI C'AATT_G Cuts at: 5979 6375 5979 Size: 396 8380 MluI A'CGCG_T Cuts at: 8652 8652 Size: 8776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2028 2425 2889 3772 7890 8074 1305 Size: 723 397 464 883 4118 184 2007 Fragments arranged by size: 4118 2007 883 723 464 397 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2798 4337 4422 10 Size: 54 1425 343 966 1539 85 4364 Fragments arranged by size: 4364 1539 1425 966 343 85 54 MslI CAynn'nnrTG Cuts at: 518 1166 3905 5522 6734 7093 7252 518 Size: 648 2739 1617 1212 359 159 2042 Fragments arranged by size: 2739 2042 1617 1212 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2129 2901 3880 3997 4529 4639 4762 4823 Size: 523 772 979 117 532 110 123 61 Cuts at: 4823 5743 6192 6869 8017 8262 1606 Size: 920 449 677 1148 245 2120 Fragments arranged by size: 2120 1148 979 920 772 677 532 523 449 245 123 117 110 61 NaeI GCC'GGC Cuts at: 3803 4094 3803 Size: 291 8485 NcoI C'CATG_G Cuts at: 513 1065 2004 2054 513 Size: 552 939 50 7235 Fragments arranged by size: 7235 939 552 50 NdeI CA'TA_TG Cuts at: 387 3381 3579 387 Size: 2994 198 5584 Fragments arranged by size: 5584 2994 198 NgoMIV G'CCGG_C Cuts at: 3801 4092 3801 Size: 291 8485 NheI G'CTAG_C Cuts at: 1052 2701 4714 1052 Size: 1649 2013 5114 Fragments arranged by size: 5114 2013 1649 NotI GC'GGCC_GC Cuts at: 6223 6223 Size: 8776 NsiI A_TGCA'T Cuts at: 3745 5648 3745 Size: 1903 6873 NspI r_CATG'y Cuts at: 1132 1229 3028 4104 5404 5521 5721 5881 6134 Size: 97 1799 1076 1300 117 200 160 253 Cuts at: 6134 7679 1132 Size: 1545 2229 Fragments arranged by size: 2229 1799 1545 1300 1076 253 200 160 117 97 PasI CC'CwG_GG Cuts at: 1348 2833 4885 1348 Size: 1485 2052 5239 Fragments arranged by size: 5239 2052 1485 PciI A'CATG_T Cuts at: 3024 7675 3024 Size: 4651 4125 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8776 PfoI T'CCnGG_A Cuts at: 2952 5238 2952 Size: 2286 6490 PmeI GTTT'AAAC Cuts at: 6080 6080 Size: 8776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4979 5011 6845 6877 8358 8390 4979 Size: 32 1834 32 1481 32 5365 Fragments arranged by size: 5365 1834 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2908 1613 Size: 1295 7481 PsiI TTA'TAA Cuts at: 2103 6346 2103 Size: 4243 4533 PspXI vC'TCGA_Gb Cuts at: 5475 5475 Size: 8776 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4956 4988 4956 Size: 32 8744 PstI C_TGCA'G Cuts at: 830 2731 3810 5289 5599 6128 830 Size: 1901 1079 1479 310 529 3478 Fragments arranged by size: 3478 1901 1479 1079 529 310 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8776 PvuII CAG'CTG Cuts at: 2129 3880 4529 4762 4823 5743 2129 Size: 1751 649 233 61 920 5162 Fragments arranged by size: 5162 1751 920 649 233 61 SalI G'TCGA_C Cuts at: 1936 6118 1936 Size: 4182 4594 SapI GCTCTTCn'nnn_ Cuts at: 4232 4533 4232 Size: 301 8475 SbfI CC_TGCA'GG Cuts at: 6128 6128 Size: 8776 ScaI AGT'ACT Cuts at: 4788 7024 8736 4788 Size: 2236 1712 4828 Fragments arranged by size: 4828 2236 1712 SexAI A'CCwGG_T Cuts at: 2764 2764 Size: 8776 SfcI C'TryA_G Cuts at: 826 1045 2088 2727 3806 4177 4644 5285 5371 Size: 219 1043 639 1079 371 467 641 86 Cuts at: 5371 5440 5595 5614 6124 7259 7940 8131 826 Size: 69 155 19 510 1135 681 191 1471 Fragments arranged by size: 1471 1135 1079 1043 681 641 639 510 467 371 219 191 155 86 69 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8776 SmaI CCC'GGG Cuts at: 1872 6103 1872 Size: 4231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4194 4411 5475 6880 7781 8043 Size: 197 926 2251 217 1064 1405 901 262 Cuts at: 8043 820 Size: 1553 Fragments arranged by size: 2251 1553 1405 1064 926 901 262 217 197 SnaBI TAC'GTA Cuts at: 493 4156 6567 493 Size: 3663 2411 2702 Fragments arranged by size: 3663 2702 2411 SpeI A'CTAG_T Cuts at: 152 2261 2404 152 Size: 2109 143 6524 Fragments arranged by size: 6524 2109 143 SphI G_CATG'C Cuts at: 1132 5521 6134 1132 Size: 4389 613 3774 Fragments arranged by size: 4389 3774 613 SspI AAT'ATT Cuts at: 5 52 2289 3364 6700 5 Size: 47 2237 1075 3336 2081 Fragments arranged by size: 3336 2237 2081 1075 47 StuI AGG'CCT Cuts at: 1517 2227 4880 1517 Size: 710 2653 5413 Fragments arranged by size: 5413 2653 710 StyI C'CwwG_G Cuts at: 513 1065 2004 2054 3822 3903 5309 6215 513 Size: 552 939 50 1768 81 1406 906 3074 Fragments arranged by size: 3074 1768 1406 939 906 552 81 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4449 4715 4936 5750 6041 6803 6820 Size: 1255 2869 266 221 814 291 762 17 Cuts at: 6820 6973 7158 7603 325 Size: 153 185 445 1498 Fragments arranged by size: 2869 1498 1255 814 762 445 291 266 221 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2332 4786 5167 Size: 274 80 33 51 1443 355 2454 381 Cuts at: 5167 7022 8734 96 Size: 1855 1712 138 Fragments arranged by size: 2454 1855 1712 1443 381 355 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3555 3587 4066 4098 5297 5329 5338 5370 8736 Size: 32 479 32 1199 32 9 32 3366 Cuts at: 8736 8768 3555 Size: 32 3563 Fragments arranged by size: 3563 3366 1199 479 32 32 32 32 32 9 Tth111I GACn'n_nGTC Cuts at: 1316 2593 6028 1316 Size: 1277 3435 4064 Fragments arranged by size: 4064 3435 1277 XbaI T'CTAG_A Cuts at: 4897 6112 4897 Size: 1215 7561 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2830 2830 Size: 8776 XhoI C'TCGA_G Cuts at: 1943 4194 5475 1943 Size: 2251 1281 5244 Fragments arranged by size: 5244 2251 1281 XmaI C'CCGG_G Cuts at: 1870 6101 1870 Size: 4231 4545 XmnI GAAnn'nnTTC Cuts at: 3498 3725 5994 6905 3498 Size: 227 2269 911 5369 Fragments arranged by size: 5369 2269 911 227 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6584 276 Size: 53 83 186 936 5050 2468 Fragments arranged by size: 5050 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI