Restriction map for
pFN21AA0456
(Circular) (Six-base) MAPSORT of: pFN21AA0456.seq Check: 4661 from: 1 to: 7918
pFN21AA0456 7918 bp
With 182 enzymes: SgfI *
January 26, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2012 5728 278
Size: 53 83 186 936 476 3716 2468
Fragments arranged by size:
3716 2468 936 476 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4071 5195 5261 1937
Size: 2134 1124 66 4594
Fragments arranged by size:
4594 2134 1124 66
Acc65I G'GTAC_C
Cuts at: 5239 5239
Size: 7918
AclI AA'CG_TT
Cuts at: 1563 2640 6045 6418 1563
Size: 1077 3405 373 3063
Fragments arranged by size:
3405 3063 1077 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2756 3719 4449 4754 5981 7365 7633 1632
Size: 1124 963 730 305 1227 1384 268 1917
Fragments arranged by size:
1917 1384 1227 1124 963 730 305 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7721
AflIII A'CryG_T
Cuts at: 1203 2508 2659 6817 7794 1203
Size: 1305 151 4158 977 1327
Fragments arranged by size:
4158 1327 1305 977 151
AgeI A'CCGG_T
Cuts at: 6749 6749
Size: 7918
AhdI GACnn_n'nnGTC
Cuts at: 3976 6647 3976
Size: 2671 5247
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7918
AlwNI CAG_nnn'CTG
Cuts at: 4532 5661 7233 4532
Size: 1129 1572 5217
Fragments arranged by size:
5217 1572 1129
ApaI G_GGCC'C
Cuts at: 3942 4067 3942
Size: 125 7793
ApaLI G'TGCA_C
Cuts at: 5975 7131 5975
Size: 1156 6762
ApoI r'AATT_y
Cuts at: 1493 2021 3576 5227 5453 5605 7714 1493
Size: 528 1555 1651 226 152 2109 1697
Fragments arranged by size:
2109 1697 1651 1555 528 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6472 160
Size: 872 5440 1606
Fragments arranged by size:
5440 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7918
AvaI C'yCGr_G
Cuts at: 1870 1943 4236 5015 5243 1870
Size: 73 2293 779 228 4545
Fragments arranged by size:
4545 2293 779 228 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2049 2082 2049
Size: 33 7885
BamHI G'GATC_C
Cuts at: 3593 5248 5615 3593
Size: 1655 367 5896
Fragments arranged by size:
5896 1655 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5239 6694 618
Size: 325 217 627 3452 1455 1842
Fragments arranged by size:
3452 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2484 2675 2878 3799 3942 4067 4194 4941
Size: 1093 191 203 921 143 125 127 747
Cuts at: 4941 4980 5022 5237 1391
Size: 39 42 215 4072
Fragments arranged by size:
4072 1093 921 747 215 203 191 143
127 125 42 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2419 2504 2788 3004 3176 4143 4772
Size: 971 520 85 284 216 172 967 629
Cuts at: 4772 5131 928
Size: 359 3715
Fragments arranged by size:
3715 971 967 629 520 359 284 216
172 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4187 4221 6109 6143 1903
Size: 34 2250 34 1888 34 3678
Fragments arranged by size:
3678 2250 1888 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 5810 7020 3548
Size: 2262 1210 4446
Fragments arranged by size:
4446 2262 1210
BclI T'GATC_A
Cuts at: 1548 2833 4005 1548
Size: 1285 1172 5461
Fragments arranged by size:
5461 1285 1172
BfrBI ATG'CAT
Cuts at: 2789 2789
Size: 7918
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6529 136
Size: 107 122 71 631 605 4857 1525
Fragments arranged by size:
4857 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7913 7913
Size: 7918
BlpI GC'TnA_GC
Cuts at: 2082 4574 5335 2082
Size: 2492 761 4665
Fragments arranged by size:
4665 2492 761
Bme1580I G_kGCm'C
Cuts at: 1790 3079 3942 4067 4698 5979 7135 1790
Size: 1289 863 125 631 1281 1156 2573
Fragments arranged by size:
2573 1289 1281 1156 863 631 125
BmgBI CAC'GTC
Cuts at: 1318 4918 4971 1318
Size: 3600 53 4265
Fragments arranged by size:
4265 3600 53
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4461 4949 5649
Size: 416 53 83 399 24 3044 488 700
Cuts at: 5649 6607 442
Size: 958 1753
Fragments arranged by size:
3044 1753 958 700 488 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4620 1056
Size: 3564 4354
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 7918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1998 2135 2225 2271 3398 3487 4079 4966
Size: 759 137 90 46 1127 89 592 887
Cuts at: 4966 6578 1239
Size: 1612 2579
Fragments arranged by size:
2579 1612 1127 887 759 592 137 90
89 46
Bpu10I CC'TnA_GC
Cuts at: 4833 4833
Size: 7918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4991 6043 6908 7206 4991
Size: 1052 865 298 5703
Fragments arranged by size:
5703 1052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3286 6581 882
Size: 617 1787 3295 2219
Fragments arranged by size:
3295 2219 1787 617
BsaAI yAC'GTr
Cuts at: 493 1204 3994 4222 5709 493
Size: 711 2790 228 1487 2702
Fragments arranged by size:
2790 2702 1487 711 228
BsaBI GATnn'nnATC
Cuts at: 4491 7912 4491
Size: 3421 4497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 5725 6107 6742
Size: 53 83 186 936 476 3716 382 635
Cuts at: 6742 275
Size: 1451
Fragments arranged by size:
3716 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2664 3540 3807 4542 4761 6351 6749 7023 7170
Size: 876 267 735 219 1590 398 274 147
Cuts at: 7170 7581 2664
Size: 411 3001
Fragments arranged by size:
3001 1590 876 735 411 398 274 267
219 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3239 3269 4583 4613 1178
Size: 30 165 30 1836 30 1314 30 4483
Fragments arranged by size:
4483 1836 1314 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2582 2666 2683 3334 3651 4095 4585
Size: 1111 285 84 17 651 317 444 490
Cuts at: 4585 4588 4994 1186
Size: 3 406 4110
Fragments arranged by size:
4110 1111 651 490 444 406 317 285
84 17 3
BseYI C'CCAG_C
Cuts at: 3066 3078 3305 3653 4635 4998 5048 7121 7617
Size: 12 227 348 982 363 50 2073 496
Cuts at: 7617 3066
Size: 3367
Fragments arranged by size:
3367 2073 982 496 363 348 227 50
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3785 4976 1163
Size: 573 2049 1191 4105
Fragments arranged by size:
4105 2049 1191 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5368 6129 7157 1540
Size: 275 186 3367 761 1028 2301
Fragments arranged by size:
3367 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2083 2484 2536 2675 3799 4167 5979 6064 7135
Size: 401 52 139 1124 368 1812 85 1071
Cuts at: 7135 2083
Size: 2866
Fragments arranged by size:
2866 1812 1124 1071 401 368 139 85
52
BsmI GAATG_Cn'
Cuts at: 2953 5429 5522 2953
Size: 2476 93 5349
Fragments arranged by size:
5349 2476 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2016 2016
Size: 7918
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2484 2536 2675 2878 3079 3799
Size: 399 293 401 52 139 203 201 720
Cuts at: 3799 3942 4067 4167 4194 4698 4941 4980 5022
Size: 143 125 100 27 504 243 39 42
Cuts at: 5022 5237 5979 6064 7135 1391
Size: 215 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 720 504 401 399 293
243 215 203 201 143 139 125 100
85 52 42 39 27
BspEI T'CCGG_A
Cuts at: 2664 3807 4542 4761 7581 2664
Size: 1143 735 219 2820 3001
Fragments arranged by size:
3001 2820 1143 735 219
BspHI T'CATG_A
Cuts at: 3294 3751 3849 4644 5807 7537 3294
Size: 457 98 795 1163 1730 3675
Fragments arranged by size:
3675 1730 1163 795 457 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3792 5273 844
Size: 2948 1481 3489
Fragments arranged by size:
3489 2948 1481
BsrBI CCG'CTC
Cuts at: 1394 1823 4451 5805 1394
Size: 429 2628 1354 3507
Fragments arranged by size:
3507 2628 1354 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3309 4203 4827 6413 6587 65
Size: 1183 2061 894 624 1586 174 1396
Fragments arranged by size:
2061 1586 1396 1183 894 624 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2604 4308 6562 6749 1700
Size: 110 794 1704 2254 187 2869
Fragments arranged by size:
2869 2254 1704 794 187 110
BsrGI T'GTAC_A
Cuts at: 96 2389 2511 3000 96
Size: 2293 122 489 5014
Fragments arranged by size:
5014 2293 489 122
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7918
BssSI C'ACGA_G
Cuts at: 4171 5978 6990 4171
Size: 1807 1012 5099
Fragments arranged by size:
5099 1807 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2140 2786 794
Size: 1346 646 5926
Fragments arranged by size:
5926 1346 646
BstBI TT'CG_AA
Cuts at: 6722 6722
Size: 7918
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7918
BstXI CCAn_nnnn'nTGG
Cuts at: 4057 4057
Size: 7918
BstYI r'GATC_y
Cuts at: 1972 2073 3593 4758 5248 5615 6000 6017 7469
Size: 101 1520 1165 490 367 385 17 1452
Cuts at: 7469 7555 7567 7913 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1520 1452 1165 490 385 367 346
101 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4821 4821
Size: 7918
BtgI C'CryG_G
Cuts at: 513 1065 2631 3258 3276 4567 4812 513
Size: 552 1566 627 18 1291 245 3619
Fragments arranged by size:
3619 1566 1291 627 552 245 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4191 5041 487
Size: 683 56 2965 850 3364
Fragments arranged by size:
3364 2965 850 683 56
BtsI GCAGTG_nn'
Cuts at: 2123 4903 5434 6228 6248 2123
Size: 2780 531 794 20 3793
Fragments arranged by size:
3793 2780 794 531 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7883
DraI TTT'AAA
Cuts at: 2422 5222 2422
Size: 2800 5118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4823 6925 809
Size: 507 3507 2102 1802
Fragments arranged by size:
3507 2102 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2440 2634
Size: 54 1425 50 275 18 399 211 194
Cuts at: 2634 5365 6254 7883 8
Size: 2731 889 1629 43
Fragments arranged by size:
2731 1629 1425 889 399 275 211 194
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5365 1537
Size: 275 3553 4090
Fragments arranged by size:
4090 3553 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3153 3737 3891 5848 1353
Size: 1800 584 154 1957 3423
Fragments arranged by size:
3423 1957 1800 584 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2570 6505 6879 7025 387
Size: 1064 138 981 3935 374 146 1280
Fragments arranged by size:
3935 1280 1064 981 374 146 138
EcoICRI GAG'CTC
Cuts at: 2482 2673 3797 2482
Size: 191 1124 6603
Fragments arranged by size:
6603 1124 191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2135 2225 2271 2756 3398 3487
Size: 393 366 137 90 46 485 642 89
Cuts at: 3487 3719 4079 4449 4754 4966 5981 6578 7365
Size: 232 360 370 305 212 1015 597 787
Cuts at: 7365 7633 1239
Size: 268 1524
Fragments arranged by size:
1524 1015 787 642 597 485 393 370
366 360 305 268 232 212 137 90
89 46
EcoO109I rG'GnC_Cy
Cuts at: 2146 2470 3643 3939 4063 4064 5011 2146
Size: 324 1173 296 124 1 947 5053
Fragments arranged by size:
5053 1173 947 324 296 124 1
EcoRI G'AATT_C
Cuts at: 5227 5605 5227
Size: 378 7540
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3724 3763 3724
Size: 39 7879
FspI TGC'GCA
Cuts at: 6424 6424
Size: 7918
HaeII r_GCGC'y
Cuts at: 1678 2989 5040 7065 1678
Size: 1311 2051 2025 2531
Fragments arranged by size:
2531 2051 2025 1311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 3962 3994 4583
Size: 32 135 32 493 32 1865 32 589
Cuts at: 4583 4615 6559 6591 6633 6665 1373
Size: 32 1944 32 42 32 2626
Fragments arranged by size:
2626 1944 1865 589 493 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2293 5173 5262 5508 669
Size: 988 281 355 2880 89 246 3079
Fragments arranged by size:
3079 2880 988 355 281 246 89
HindIII A'AGCT_T
Cuts at: 748 3701 4792 748
Size: 2953 1091 3874
Fragments arranged by size:
3874 2953 1091
HpaI GTT'AAC
Cuts at: 5508 5508
Size: 7918
KpnI G_GTAC'C
Cuts at: 5243 5243
Size: 7918
MfeI C'AATT_G
Cuts at: 5517 5517
Size: 7918
MluI A'CGCG_T
Cuts at: 7794 7794
Size: 7918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4314 7032 7216 1305
Size: 3009 2718 184 2007
Fragments arranged by size:
3009 2718 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2231 10
Size: 54 1425 343 399 5697
Fragments arranged by size:
5697 1425 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2996 3362 3996 5876 6235 6394 518
Size: 648 1830 366 634 1880 359 159 2042
Fragments arranged by size:
2042 1880 1830 648 634 366 359 159
MspA1I CmG'CkG
Cuts at: 1606 3451 4282 4461 4778 5334 6011 7159 7404
Size: 1845 831 179 317 556 677 1148 245
Cuts at: 7404 1606
Size: 2120
Fragments arranged by size:
2120 1845 1148 831 677 556 317 245
179
NaeI GCC'GGC
Cuts at: 4310 4310
Size: 7918
NcoI C'CATG_G
Cuts at: 513 1065 3258 4567 4812 513
Size: 552 2193 1309 245 3619
Fragments arranged by size:
3619 2193 1309 552 245
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7918
NgoMIV G'CCGG_C
Cuts at: 4308 4308
Size: 7918
NheI G'CTAG_C
Cuts at: 1052 4616 1052
Size: 3564 4354
NotI GC'GGCC_GC
Cuts at: 5365 5365
Size: 7918
NsiI A_TGCA'T
Cuts at: 2791 2791
Size: 7918
NspI r_CATG'y
Cuts at: 1132 1229 2512 2663 5276 6821 1132
Size: 97 1283 151 2613 1545 2229
Fragments arranged by size:
2613 2229 1545 1283 151 97
PasI CC'CwG_GG
Cuts at: 1348 3436 4050 4604 1348
Size: 2088 614 554 4662
Fragments arranged by size:
4662 2088 614 554
PciI A'CATG_T
Cuts at: 2508 2659 6817 2508
Size: 151 4158 3609
Fragments arranged by size:
4158 3609 151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3676 4532 1108
Size: 2568 856 4494
Fragments arranged by size:
4494 2568 856
PfoI T'CCnGG_A
Cuts at: 2151 3198 2151
Size: 1047 6871
PmeI GTTT'AAAC
Cuts at: 5222 5222
Size: 7918
PmlI CAC'GTG
Cuts at: 3994 3994
Size: 7918
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3714 3746 5987 6019 7500 7532 3714
Size: 32 2241 32 1481 32 4100
Fragments arranged by size:
4100 2241 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 2470 3643 5011 2146
Size: 324 1173 1368 5053
Fragments arranged by size:
5053 1368 1173 324
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7918
PsiI TTA'TAA
Cuts at: 5488 5488
Size: 7918
PspOMI G'GGCC_C
Cuts at: 3938 4063 3938
Size: 125 7793
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2468 2500 2468
Size: 32 7886
PstI C_TGCA'G
Cuts at: 830 3126 3429 3789 5270 830
Size: 2296 303 360 1481 3478
Fragments arranged by size:
3478 2296 1481 360 303
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7918
PvuII CAG'CTG
Cuts at: 4461 4461
Size: 7918
SacI G_AGCT'C
Cuts at: 2484 2675 3799 2484
Size: 191 1124 6603
Fragments arranged by size:
6603 1124 191
SalI G'TCGA_C
Cuts at: 1936 5260 1936
Size: 3324 4594
SbfI CC_TGCA'GG
Cuts at: 3789 5270 3789
Size: 1481 6437
ScaI AGT'ACT
Cuts at: 2762 2804 5044 6166 7878 2762
Size: 42 2240 1122 1712 2802
Fragments arranged by size:
2802 2240 1712 1122 42
SexAI A'CCwGG_T
Cuts at: 2316 3972 2316
Size: 1656 6262
SfcI C'TryA_G
Cuts at: 826 1045 3122 3425 3563 3785 4072 5266 6401
Size: 219 2077 303 138 222 287 1194 1135
Cuts at: 6401 7082 7273 826
Size: 681 191 1471
Fragments arranged by size:
2077 1471 1194 1135 681 303 287 222
219 191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7918
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7918
SmaI CCC'GGG
Cuts at: 1872 5245 1872
Size: 3373 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5006 6022 6923 7185 820
Size: 197 926 3063 1016 901 262 1553
Fragments arranged by size:
3063 1553 1016 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5709 493
Size: 5216 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7918
SphI G_CATG'C
Cuts at: 1132 5276 1132
Size: 4144 3774
SspI AAT'ATT
Cuts at: 5 52 5842 5
Size: 47 5790 2081
Fragments arranged by size:
5790 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7918
StyI C'CwwG_G
Cuts at: 513 1065 2247 3258 3738 4567 4812 4932 5357
Size: 552 1182 1011 480 829 245 120 425
Cuts at: 5357 513
Size: 3074
Fragments arranged by size:
3074 1182 1011 829 552 480 425 245
120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5012 5945 5962 6115 6300 6745 325
Size: 1255 3432 933 17 153 185 445 1498
Fragments arranged by size:
3432 1498 1255 933 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2389 2511 2718
Size: 274 80 33 51 1443 412 122 207
Cuts at: 2718 2760 2802 3000 3246 4344 5042 5210 6164
Size: 42 42 198 246 1098 698 168 954
Cuts at: 6164 7876 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1098 954 698 412 274 246
207 198 168 138 122 80 51 42
42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2102 2134 3585 3617 7878 7910 2102
Size: 32 1451 32 4261 32 2110
Fragments arranged by size:
4261 2110 1451 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4373 1316
Size: 3057 4861
XbaI T'CTAG_A
Cuts at: 5254 5254
Size: 7918
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2295 2295
Size: 7918
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7918
XmaI C'CCGG_G
Cuts at: 1870 5243 1870
Size: 3373 4545
XmnI GAAnn'nnTTC
Cuts at: 4037 6047 4037
Size: 2010 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2010 5726 276
Size: 53 83 186 936 476 3716 2468
Fragments arranged by size:
3716 2468 936 476 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BsiWI
BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NarI
NruI PacI PspXI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI