(Circular) (Six-base) MAPSORT of: pFN21AA0318.seq Check: 6421 from: 1 to: 7861 pFN21AA0318 7861 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3201 3201 Size: 7861 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5671 278 Size: 53 83 186 936 4135 2468 Fragments arranged by size: 4135 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5204 1937 Size: 3267 4594 Acc65I G'GTAC_C Cuts at: 5182 5182 Size: 7861 AclI AA'CG_TT Cuts at: 1563 3573 5988 6361 1563 Size: 2010 2415 373 3063 Fragments arranged by size: 3063 2415 2010 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3636 5924 7308 7576 1632 Size: 2004 2288 1384 268 1917 Fragments arranged by size: 2288 2004 1917 1384 268 AfeI AGC'GCT Cuts at: 2323 4432 2323 Size: 2109 5752 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7664 AflIII A'CryG_T Cuts at: 1203 6760 7737 1203 Size: 5557 977 1327 Fragments arranged by size: 5557 1327 977 AgeI A'CCGG_T Cuts at: 6692 6692 Size: 7861 AhdI GACnn_n'nnGTC Cuts at: 2710 6590 2710 Size: 3880 3981 AleI CACnn'nnGTG Cuts at: 1166 2517 3647 3992 1166 Size: 1351 1130 345 5035 Fragments arranged by size: 5035 1351 1130 345 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3122 3906 1184 Size: 1938 784 5139 Fragments arranged by size: 5139 1938 784 AlwNI CAG_nnn'CTG Cuts at: 2035 2059 3740 4385 4526 4613 5604 7176 2035 Size: 24 1681 645 141 87 991 1572 2720 Fragments arranged by size: 2720 1681 1572 991 645 141 87 24 ApaI G_GGCC'C Cuts at: 3938 3938 Size: 7861 ApaLI G'TGCA_C Cuts at: 3148 3891 5918 7074 3148 Size: 743 2027 1156 3935 Fragments arranged by size: 3935 2027 1156 743 ApoI r'AATT_y Cuts at: 1493 2473 5170 5396 5548 7657 1493 Size: 980 2697 226 152 2109 1697 Fragments arranged by size: 2697 2109 1697 980 226 152 AseI AT'TA_AT Cuts at: 160 1032 6415 160 Size: 872 5383 1606 Fragments arranged by size: 5383 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7861 AvaI C'yCGr_G Cuts at: 1870 1943 2169 2500 3752 3781 4023 5186 1870 Size: 73 226 331 1252 29 242 1163 4545 Fragments arranged by size: 4545 1252 1163 331 242 226 73 29 BamHI G'GATC_C Cuts at: 5191 5558 5191 Size: 367 7494 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2674 2968 3763 3986 4160 Size: 325 217 627 887 294 795 223 174 Cuts at: 4160 4535 5182 6637 618 Size: 375 647 1455 1842 Fragments arranged by size: 1842 1455 887 795 647 627 375 325 294 223 217 174 BanII G_rGCy'C Cuts at: 1391 2652 2961 3759 3938 4548 5180 1391 Size: 1261 309 798 179 610 632 4072 Fragments arranged by size: 4072 1261 798 632 610 309 179 BbeI G_GCGC'C Cuts at: 4164 4164 Size: 7861 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2892 4069 928 Size: 971 993 1177 4720 Fragments arranged by size: 4720 1177 993 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2828 2862 6052 6086 1903 Size: 34 50 34 807 34 3190 34 3678 Fragments arranged by size: 3678 3190 807 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5753 6963 5753 Size: 1210 6651 BclI T'GATC_A Cuts at: 1548 1548 Size: 7861 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3553 4028 6472 Size: 107 122 71 631 605 1881 475 2444 Cuts at: 6472 136 Size: 1525 Fragments arranged by size: 2444 1881 1525 631 605 475 122 107 71 BglII A'GATC_T Cuts at: 2444 3216 7856 2444 Size: 772 4640 2449 Fragments arranged by size: 4640 2449 772 BlpI GC'TnA_GC Cuts at: 2574 5278 2574 Size: 2704 5157 Bme1580I G_kGCm'C Cuts at: 1790 2679 3152 3895 3915 3938 3989 4708 5034 Size: 889 473 743 20 23 51 719 326 Cuts at: 5034 5922 7078 1790 Size: 888 1156 2573 Fragments arranged by size: 2573 1156 889 888 743 719 473 326 51 23 20 BmgBI CAC'GTC Cuts at: 1318 2863 3172 3265 4347 1318 Size: 1545 309 93 1082 4832 Fragments arranged by size: 4832 1545 1082 309 93 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5592 6550 442 Size: 416 53 83 399 24 4175 958 1753 Fragments arranged by size: 4175 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7861 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2669 2669 Size: 7861 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2801 3425 3547 3695 3840 3941 4125 Size: 959 603 624 122 148 145 101 184 Cuts at: 4125 4485 6521 1239 Size: 360 2036 2579 Fragments arranged by size: 2579 2036 959 624 603 360 184 148 145 122 101 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4346 5088 5986 6851 7149 4346 Size: 742 898 865 298 5058 Fragments arranged by size: 5058 898 865 742 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3523 3526 3778 4482 4741 6524 882 Size: 617 2024 3 252 704 259 1783 2219 Fragments arranged by size: 2219 2024 1783 704 617 259 252 3 BsaAI yAC'GTr Cuts at: 493 1204 5652 493 Size: 711 4448 2702 Fragments arranged by size: 4448 2702 711 BsaBI GATnn'nnATC Cuts at: 2443 7855 2443 Size: 5412 2449 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3545 4128 4161 4849 Size: 53 83 186 936 2012 583 33 688 Cuts at: 4849 5668 6050 6685 275 Size: 819 382 635 1451 Fragments arranged by size: 2012 1451 936 819 688 635 583 382 186 83 53 33 BsaWI w'CCGG_w Cuts at: 2426 6294 6692 6966 7113 7524 2426 Size: 3868 398 274 147 411 2763 Fragments arranged by size: 3868 2763 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2381 2411 2792 2822 3086 Size: 30 165 30 978 30 381 30 264 Cuts at: 3086 3116 4280 4310 1178 Size: 30 1164 30 4729 Fragments arranged by size: 4729 1164 978 381 264 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2642 3296 3299 4136 4168 4292 4301 4304 Size: 1456 654 3 837 32 124 9 3 Cuts at: 4304 4634 4637 4775 1186 Size: 330 3 138 4272 Fragments arranged by size: 4272 1456 837 654 330 138 124 32 9 3 3 3 BseYI C'CCAG_C Cuts at: 2215 2258 2731 2952 3040 3225 3463 3494 3959 Size: 43 473 221 88 185 238 31 465 Cuts at: 3959 4337 4378 7064 7560 2215 Size: 378 41 2686 496 2516 Fragments arranged by size: 2686 2516 496 473 465 378 238 221 185 88 43 41 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2720 3073 3128 3774 1163 Size: 573 984 353 55 646 5250 Fragments arranged by size: 5250 984 646 573 353 55 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2346 3648 3950 5311 6072 7100 Size: 275 186 345 1302 302 1361 761 1028 Cuts at: 7100 1540 Size: 2301 Fragments arranged by size: 2301 1361 1302 1028 761 345 302 275 186 BsiHKAI G_wGCw'C Cuts at: 2652 2793 3152 3759 3846 3895 4548 5922 6007 Size: 141 359 607 87 49 653 1374 85 Cuts at: 6007 7078 2652 Size: 1071 3435 Fragments arranged by size: 3435 1374 1071 653 607 359 141 87 85 49 BsmI GAATG_Cn' Cuts at: 5372 5465 5372 Size: 93 7768 BsmBI CGTCTCn'nnnn_ Cuts at: 4218 4218 Size: 7861 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2652 2679 2793 2961 3152 3759 3846 Size: 399 862 27 114 168 191 607 87 Cuts at: 3846 3895 3915 3938 3989 4548 4708 5034 5180 Size: 49 20 23 51 559 160 326 146 Cuts at: 5180 5922 6007 7078 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 862 742 607 559 399 326 191 168 160 146 114 87 85 51 49 27 23 20 BspEI T'CCGG_A Cuts at: 7524 7524 Size: 7861 BspHI T'CATG_A Cuts at: 2280 5750 7480 2280 Size: 3470 1730 2661 Fragments arranged by size: 3470 2661 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 2220 3201 3796 4047 5216 844 Size: 1325 51 981 595 251 1169 3489 Fragments arranged by size: 3489 1325 1169 981 595 251 51 BsrBI CCG'CTC Cuts at: 1394 1823 4588 5748 1394 Size: 429 2765 1160 3507 Fragments arranged by size: 3507 2765 1160 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6356 6530 65 Size: 1183 5108 174 1396 Fragments arranged by size: 5108 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3578 6505 6692 1700 Size: 110 1768 2927 187 2869 Fragments arranged by size: 2927 2869 1768 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7861 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7861 BssSI C'ACGA_G Cuts at: 5921 6933 5921 Size: 1012 6849 BstAPI GCAn_nnn'nTGC Cuts at: 794 2035 3740 794 Size: 1241 1705 4915 Fragments arranged by size: 4915 1705 1241 BstBI TT'CG_AA Cuts at: 6665 6665 Size: 7861 BstEII G'GTnAC_C Cuts at: 1184 3499 1184 Size: 2315 5546 BstXI CCAn_nnnn'nTGG Cuts at: 2689 2979 4018 2689 Size: 290 1039 6532 Fragments arranged by size: 6532 1039 290 BstYI r'GATC_y Cuts at: 1972 2423 2444 3216 4557 5191 5558 5943 5960 Size: 451 21 772 1341 634 367 385 17 Cuts at: 5960 7412 7498 7510 7856 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1341 772 634 451 385 367 346 86 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2250 3389 3630 3648 3723 3930 4017 Size: 552 1185 1139 241 18 75 207 87 Cuts at: 4017 4219 4423 4690 513 Size: 202 204 267 3684 Fragments arranged by size: 3684 1185 1139 552 267 241 207 204 202 87 75 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7122 Fragments arranged by size: 7122 683 56 BtsI GCAGTG_nn' Cuts at: 3116 3152 5377 6171 6191 3116 Size: 36 2225 794 20 4786 Fragments arranged by size: 4786 2225 794 36 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7861 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7826 DraI TTT'AAA Cuts at: 4636 5165 4636 Size: 529 7332 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5069 6868 809 Size: 507 3753 1799 1802 Fragments arranged by size: 3753 1802 1799 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2659 3369 3645 Size: 54 1425 50 275 18 829 710 276 Cuts at: 3645 4044 5308 6197 7826 8 Size: 399 1264 889 1629 43 Fragments arranged by size: 1629 1425 1264 889 829 710 399 276 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3645 5308 1537 Size: 275 1833 1663 4090 Fragments arranged by size: 4090 1833 1663 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 4509 4536 5135 5791 1353 Size: 768 2388 27 599 656 3423 Fragments arranged by size: 3423 2388 768 656 599 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2389 3211 3697 6448 6822 6968 Size: 1064 138 800 822 486 2751 374 146 Cuts at: 6968 387 Size: 1280 Fragments arranged by size: 2751 1280 1064 822 800 486 374 146 138 EcoICRI GAG'CTC Cuts at: 2650 3757 4546 2650 Size: 1107 789 5965 Fragments arranged by size: 5965 1107 789 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2801 3425 3547 3636 3695 3840 Size: 393 566 603 624 122 89 59 145 Cuts at: 3840 3941 4125 4485 5924 6521 7308 7576 1239 Size: 101 184 360 1439 597 787 268 1524 Fragments arranged by size: 1524 1439 787 624 603 597 566 393 360 268 184 145 122 101 89 59 EcoNI CCTnn'n_nnAGG Cuts at: 3278 3278 Size: 7861 EcoO109I rG'GnC_Cy Cuts at: 2056 2173 2853 3458 3851 3935 4446 2056 Size: 117 680 605 393 84 511 5471 Fragments arranged by size: 5471 680 605 511 393 117 84 EcoRI G'AATT_C Cuts at: 2473 5170 5548 2473 Size: 2697 378 4786 Fragments arranged by size: 4786 2697 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2428 3445 5093 2428 Size: 1017 1648 5196 Fragments arranged by size: 5196 1648 1017 FspI TGC'GCA Cuts at: 6367 6367 Size: 7861 HaeII r_GCGC'y Cuts at: 1678 2325 4164 4434 7008 1678 Size: 647 1839 270 2574 2531 Fragments arranged by size: 2574 2531 1839 647 270 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2696 2728 2778 Size: 32 135 32 809 32 283 32 50 Cuts at: 2778 2792 2810 2824 2874 2906 4756 4788 5075 Size: 14 18 14 50 32 1850 32 287 Cuts at: 5075 5107 6502 6534 6576 6608 1373 Size: 32 1395 32 42 32 2626 Fragments arranged by size: 2626 1850 1395 809 287 283 135 50 50 42 32 32 32 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 5205 5451 669 Size: 988 281 3267 246 3079 Fragments arranged by size: 3267 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7861 HpaI GTT'AAC Cuts at: 5451 5451 Size: 7861 KasI G'GCGC_C Cuts at: 4160 4160 Size: 7861 KpnI G_GTAC'C Cuts at: 5186 5186 Size: 7861 MfeI C'AATT_G Cuts at: 5460 5460 Size: 7861 MluI A'CGCG_T Cuts at: 7737 7737 Size: 7861 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2022 2706 6975 7159 1305 Size: 717 684 4269 184 2007 Fragments arranged by size: 4269 2007 717 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2661 3371 10 Size: 54 1425 343 829 710 4500 Fragments arranged by size: 4500 1425 829 710 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2517 2823 2977 3104 3647 3992 5819 Size: 648 1351 306 154 127 543 345 1827 Cuts at: 5819 6178 6337 518 Size: 359 159 2042 Fragments arranged by size: 2042 1827 1351 648 543 359 345 306 159 154 127 MspA1I CmG'CkG Cuts at: 1606 2035 3403 4312 4341 5277 5954 7102 7347 Size: 429 1368 909 29 936 677 1148 245 Cuts at: 7347 1606 Size: 2120 Fragments arranged by size: 2120 1368 1148 936 909 677 429 245 29 NarI GG'CG_CC Cuts at: 4161 4161 Size: 7861 NcoI C'CATG_G Cuts at: 513 1065 4017 4219 513 Size: 552 2952 202 4155 Fragments arranged by size: 4155 2952 552 202 NdeI CA'TA_TG Cuts at: 387 387 Size: 7861 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7861 NotI GC'GGCC_GC Cuts at: 5308 5308 Size: 7861 NspI r_CATG'y Cuts at: 1132 1229 3919 5219 6764 1132 Size: 97 2690 1300 1545 2229 Fragments arranged by size: 2690 2229 1545 1300 97 PasI CC'CwG_GG Cuts at: 1348 3712 1348 Size: 2364 5497 PciI A'CATG_T Cuts at: 6760 6760 Size: 7861 PflMI CCAn_nnn'nTGG Cuts at: 1108 2059 1108 Size: 951 6910 PfoI T'CCnGG_A Cuts at: 2796 3524 2796 Size: 728 7133 PmeI GTTT'AAAC Cuts at: 5165 5165 Size: 7861 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2618 2650 3272 3304 4367 4399 5930 5962 7443 Size: 32 622 32 1063 32 1531 32 1481 Cuts at: 7443 7475 2618 Size: 32 3004 Fragments arranged by size: 3004 1531 1481 1063 622 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2173 2853 3458 3851 4446 2173 Size: 680 605 393 595 5588 Fragments arranged by size: 5588 680 605 595 393 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7861 PsiI TTA'TAA Cuts at: 5431 5431 Size: 7861 PspOMI G'GGCC_C Cuts at: 3934 3934 Size: 7861 PstI C_TGCA'G Cuts at: 830 2040 2103 3147 3457 3736 4390 4618 5213 Size: 1210 63 1044 310 279 654 228 595 Cuts at: 5213 830 Size: 3478 Fragments arranged by size: 3478 1210 1044 654 595 310 279 228 63 PvuI CG_AT'CG Cuts at: 2001 2346 2001 Size: 345 7516 PvuII CAG'CTG Cuts at: 2035 4312 2035 Size: 2277 5584 SacI G_AGCT'C Cuts at: 2652 3759 4548 2652 Size: 1107 789 5965 Fragments arranged by size: 5965 1107 789 SalI G'TCGA_C Cuts at: 1936 5203 1936 Size: 3267 4594 SanDI GG'GwC_CC Cuts at: 2853 3851 2853 Size: 998 6863 SapI GCTCTTCn'nnn_ Cuts at: 4536 4536 Size: 7861 SbfI CC_TGCA'GG Cuts at: 3457 5213 3457 Size: 1756 6105 ScaI AGT'ACT Cuts at: 6109 7821 6109 Size: 1712 6149 SfcI C'TryA_G Cuts at: 826 1045 2036 2099 2530 3143 3256 3453 3732 Size: 219 991 63 431 613 113 197 279 Cuts at: 3732 4386 4614 5209 6344 7025 7216 826 Size: 654 228 595 1135 681 191 1471 Fragments arranged by size: 1471 1135 991 681 654 613 595 431 279 228 219 197 191 113 63 SfiI GGCCn_nnn'nGGCC Cuts at: 4028 4028 Size: 7861 SfoI GGC'GCC Cuts at: 4162 4162 Size: 7861 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7861 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7861 SmaI CCC'GGG Cuts at: 1872 2171 4025 5188 1872 Size: 299 1854 1163 4545 Fragments arranged by size: 4545 1854 1163 299 SmlI C'TyrA_G Cuts at: 820 1017 1943 4361 5103 5965 6866 7128 820 Size: 197 926 2418 742 862 901 262 1553 Fragments arranged by size: 2418 1553 926 901 862 742 262 197 SnaBI TAC'GTA Cuts at: 493 5652 493 Size: 5159 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7861 SphI G_CATG'C Cuts at: 1132 5219 1132 Size: 4087 3774 SspI AAT'ATT Cuts at: 5 52 5785 5 Size: 47 5733 2081 Fragments arranged by size: 5733 2081 47 StuI AGG'CCT Cuts at: 1517 3428 3875 5025 1517 Size: 1911 447 1150 4353 Fragments arranged by size: 4353 1911 1150 447 StyI C'CwwG_G Cuts at: 513 1065 2109 3681 4011 4017 4219 5300 513 Size: 552 1044 1572 330 6 202 1081 3074 Fragments arranged by size: 3074 1572 1081 1044 552 330 202 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2207 2569 3973 5888 5905 6058 6243 Size: 1255 627 362 1404 1915 17 153 185 Cuts at: 6243 6688 325 Size: 445 1498 Fragments arranged by size: 1915 1498 1404 1255 627 445 362 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6107 7819 96 Size: 274 80 33 51 1443 4130 1712 138 Fragments arranged by size: 4130 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2131 2163 5128 5160 7821 7853 2131 Size: 32 2965 32 2661 32 2139 Fragments arranged by size: 2965 2661 2139 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3296 1316 Size: 1980 5881 XbaI T'CTAG_A Cuts at: 5197 5197 Size: 7861 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7861 XmaI C'CCGG_G Cuts at: 1870 2169 4023 5186 1870 Size: 299 1854 1163 4545 Fragments arranged by size: 4545 1854 1163 299 XmnI GAAnn'nnTTC Cuts at: 5990 5990 Size: 7861 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5669 276 Size: 53 83 186 936 4135 2468 Fragments arranged by size: 4135 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SrfI SwaI XcmI