Restriction map for
pFN21AA0291
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BcgI BciVI
BclI BfrI BlnI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MfeI MluI MseI MunI NaeI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccIII 1494 TCCGGA T^CCGGA
AhdI 933 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2089 GTGCAC G^TGCAC
ApaLI 2089 GTGCAC G^TGCAC
AspEI 933 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2541 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 607 GGATCC G^GATCC
BbrPI 1927 CACGTG CAC^GTG
BbvCI 1138 GCTGAGG CCTCAGC(-5/-2)
BlpI 2905 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2905 GCTCAGC GC^T[ACGT]AGC
BsaBI 855 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1494 TCCGGA T^CCGGA
BsmI 513 GCATTC GAATGC(1/-1)
BspEI 1494 TCCGGA T^CCGGA
BsrDI 509 CATTGC GCAATG(2/0)
BstAPI 169 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2905 GCTCAGC GC^T[ACGT]AGC
Eam1105I 933 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MamI 855 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1494 TCCGGA T^CCGGA
NheI 106 GCTAGC G^CTAGC
PflFI 2541 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 474 CCACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1927 CACGTG CAC^GTG
PmlI 1927 CACGTG CAC^GTG
SbfI 2376 CCTGCAGG CCTGCA^GG
SexAI 799 ACCAGGT A^CC[AT]GGT
SfiI 1602 GGCCCTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2376 CCTGCAGG CCTGCA^GG
StuI 1610 AGGCCT AGG^CCT
Tsp509I 2971 AATT ^AATT
Tth111I 2541 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 474 CCACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BalI 911 TGGCCA TGG^CCA
1145 TGGCCA
BfaI 107 CTAG C^TAG
2578 CTAG
BglII 1049 AGATCT A^GATCT
2159 AGATCT
BsaAI 709 TACGTG [CT]AC^GT[GA]
1927 CACGTG
BsaWI 1494 TCCGGA [AT]^CCGG[AT]
2974 TCCGGT
BsgI 130 CTGCAC GTGCAG(16/14)
283 GTGCAG
BsmBI 298 GAGACG CGTCTC(1/5)
2344 GAGACG
BspMI 653 ACCTGC ACCTGC(4/8)
2910 GCAGGT
BsrFI 592 ACCGGC [GA]^CCGG[CT]
2036 ACCGGC
BssSI 2092 CACGAG CACGAG(-5/-1)
1258 CTCGTG
Cfr10I 592 ACCGGC [GA]^CCGG[CT]
2036 ACCGGC
DraIII 475 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
847 CACAAAGTG
EarI 832 CTCTTC CTCTTC(1/4)
1029 CTCTTC
EcoNI 1191 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2502 CCTCCTGAAGG
Ksp632I 832 CTCTTC CTCTTC(1/4)
1029 CTCTTC
MaeI 107 CTAG C^TAG
2578 CTAG
MluNI 911 TGGCCA TGG^CCA
1145 TGGCCA
MscI 911 TGGCCA TGG^CCA
1145 TGGCCA
NcoI 56 CCATGG C^CATGG
914 CCATGG
SacI 1426 GAGCTC GAGCT^C
1456 GAGCTC
Tsp45I 2410 GTGAC ^GT[GC]AC
2657 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TaqI TseI TspRI TthHB8I XcmI
XhoII